Abstract:
:The binding of a T cell-presented peptide to MHC class II alpha,beta chains occurs as a concurrent process with the release of the associated invariant chain (Ii) by cathepsin B. Ii was digested by cathepsin B from solubilized, MHC class II alpha,beta,Ii complexes in the presence of N-hydroxysuccinimidyl-4-azidobenzoate-conjugated, 125I-labeled, influenza virus matrix (18-29) peptide. The peptide was crosslinked where it became bound. This HLA-DR1-restricted peptide bound about three times more efficiently to class II alpha,beta chains of DR1-positive B cells when present during cathepsin B digestion of Ii than when added afterward, also at pH 5.0. Binding was competed by similarly DR-restricted peptides. Cathepsin D cleaved Ii but did not enhance peptide binding. However, a trace level of cathepsin D, added to the assay for peptide binding in the presence of cathepsin B, further enhanced peptide binding about three times. These experiments support an hypothesis for the staged release of Ii fragments by cathepsin D and cathepsin B, catalyzing at one point the insertion of a peptide into the antigen binding site formed by class II alpha and beta chains.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Daibata M,Xu M,Humphreys RE,Reyes VEdoi
10.1016/0161-5890(94)90122-8subject
Has Abstractpub_date
1994-03-01 00:00:00pages
255-60issue
4eissn
0161-5890issn
1872-9142journal_volume
31pub_type
杂志文章abstract::Electron microscopy of specimens of C9 tilted through 90 degrees visualized this protein to be a globular ellipsoid with dimensions of 77 x 70 x 52 A. To check the congruence of this observation with physical properties of the molecule, hydrodynamic parameters for C9 were determined. From this work a frictional ratio ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90156-6
更新日期:1993-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(95)00068-2
更新日期:1995-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(91)90181-i
更新日期:1991-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.molimm.2016.02.008
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.04.003
更新日期:2013-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.molimm.2008.02.020
更新日期:2008-06-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.01.001
更新日期:2012-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2007-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90083-4
更新日期:1983-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.molimm.2009.11.002
更新日期:2010-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.08.004
更新日期:2007-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2004.06.019
更新日期:2005-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.12.010
更新日期:2021-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.09.029
更新日期:2009-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(84)90023-3
更新日期:1984-08-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.11.011
更新日期:2008-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0161-5890(02)00019-6
更新日期:2002-05-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2012-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2018-09-01 00:00:00
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journal_title:Molecular immunology
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更新日期:2011-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.12.005
更新日期:2006-05-01 00:00:00
abstract:OBJECTIVE:Nuclear components targeted by autoantibodies are a characteristic feature of the autoimmune disease systemic lupus erythematosus (SLE). The nucleosome, a major autoantigen, is released in patients with SLE as a result of a disturbed apoptosis and/or an insufficient clearance of apoptotic debris. During apopt...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.08.003
更新日期:2010-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.01.009
更新日期:2008-05-01 00:00:00
abstract::Although the lambda-bearing antibodies represent only 5% of the total mouse serum immunoglobulins, some antigens such as B1355 dextran (alpha (1-3)Dex), the 4-hydroxy-3-nitrophenyl acetyl (NP) and 2,4-dinitro or 2,4,6-trinitrophenyl (DNP/TNP) antigens can induce lambda-positive immune responses. In contrast to the lam...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90061-2
更新日期:1988-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2015.12.011
更新日期:2016-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.10.010
更新日期:2015-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(84)90074-9
更新日期:1984-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.03.008
更新日期:2009-06-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2003.09.001
更新日期:2003-12-01 00:00:00