Abstract:
:The DNA vaccine based on the glycoprotein gene of Infectious hematopoietic necrosis virus induces a non-specific anti-viral immune response and long-term specific immunity against IHNV. This study characterized gene expression responses associated with the early anti-viral response. Homozygous rainbow trout were injected intra-muscularly (I.M.) with vector DNA or the IHNV DNA vaccine. Gene expression in muscle tissue (I.M. site) was evaluated using a 16,008 feature salmon cDNA microarray. Eighty different genes were significantly modulated in the vector DNA group while 910 genes were modulated in the IHNV DNA vaccinate group relative to control group. Quantitative reverse-transcriptase PCR was used to examine expression of selected immune genes at the I.M. site and in other secondary tissues. In the localized response (I.M. site), the magnitudes of gene expression changes were much greater in the vaccinate group relative to the vector DNA group for the majority of genes analyzed. At secondary systemic sites (e.g. gill, kidney and spleen), type I IFN-related genes were up-regulated in only the IHNV DNA vaccinated group. The results presented here suggest that the IHNV DNA vaccine induces up-regulation of the type I IFN system across multiple tissues, which is the functional basis of early anti-viral immunity.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Purcell MK,Nichols KM,Winton JR,Kurath G,Thorgaard GH,Wheeler P,Hansen JD,Herwig RP,Park LKdoi
10.1016/j.molimm.2005.12.005subject
Has Abstractpub_date
2006-05-01 00:00:00pages
2089-106issue
13eissn
0161-5890issn
1872-9142pii
S0161-5890(05)00446-3journal_volume
43pub_type
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