Identification and characterization of a novel nanobody against human placental growth factor to modulate angiogenesis.


:Placental growth factor (PlGF), a member of vascular endothelial growth factors (VEGF) family, is considered as an important antigen associated with pathological conditions such as cancer cell growth, and metastasis. PlGF-targeting via nanobody (Nb) therefore could be beneficial to modulate these pathologies. In this work, phage-display and computational approach was employed to develop a high affinity PlGF-specific Nb. An Nb library was constructed against human recombinant PlGF (rPlGF). After panning on immobilized rPlGF the periplasmic-extract (PE) of individual colonies were screened by ELISA (PE-ELISA). The 3D structures of selected Nbs were then homology modeled and energy minimized using the AMBER force field. Binding score calculations were also assessed to reveal possible Nb-PlGF interactions. Via ELISA-based affinity/specificity determinations, the best-qualified Nb was further evaluated by proliferation, migration, 3D capillary formation, invasion assays and on Chick chorioallantoic membrane (CAM) model. An immune library of 1.5×107 individual Nb clones was constructed. By PE-ELISA 12 clones with strong signals were selected. Three out of 12 sequenced Nbs (Nb-C13, Nb-C18 and Nb-C62) showed high binding scores ranging between -378.7 and -461kcal/mol. Compared to a control Nb, Nb-C18 significantly inhibited proliferation, migration and the 3D-capillary formation of HUVEC cells (p<0.05) with an EC50 of 35nM, 42nM and 24nM and invasion of MDA-MB231was significantly suppressed (p<0.05) with an EC50 of57nM. The result of the CAM assay shows that Nb-C18 could inhibit the vascular formation in the chicken chorioallantoic membrane. This Nb can be used as anti-angiogenesis agent in future.


Mol Immunol


Molecular immunology


Arezumand R,Mahdian R,Zeinali S,Hassanzadeh-Ghassabeh G,Mansouri K,Khanahmad H,Namvar-Asl N,Rahimi H,Behdani M,Cohan RA,Eavazalipour M,Ramazani A,Muyldermans S




Has Abstract


2016-10-01 00:00:00












  • Interaction of calcium and Ro60: increase of antigenicity.

    abstract::The structural and functional integrity of the cell is largely maintained by protein-protein interactions. Recently, we demonstrated that multiple antigenic peptides (MAPs) constructed from 60 kDa Ro sequence could be used to show intramolecular and intermolecular protein-protein interaction within the 60 kDa Ro ribon...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Scofield RH,Kurien BT,Gross JK,Reed NB,Taylor AK,Dominguez N,Mehta P,Guthridge JM,Bachmann M

    更新日期:2004-07-01 00:00:00

  • TET mediated epigenetic regulation of iNKT cell lineage fate choice and function.

    abstract::During the last years, intensive research has shed light in the transcriptional networks that shape the invariant NKT (iNKT) cell lineage and guide the choices towards functionally distinct iNKT cell subsets (Constantinides and Bendelac, 2013; Engel and Kronenberg, 2014; Gapin, 2016; Kim et al., 2015). However, the ep...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Tsagaratou A

    更新日期:2018-09-01 00:00:00

  • Leucine-rich repeat-containing G-protein-coupled receptor 2 (LGR2) can regulate PO activity and AMP genes expression in Macrobrachium nipponense.

    abstract::Leucine-rich repeat-containing G-protein-coupled receptors (LGRs) form a subfamily of the large superfamily of G-protein-coupled receptors. LGRs can be divided into three groups. LGR2 from Drosophila melanogaster is involved in cuticle tanning (melanization and sclerotization). In this study, one LGR2 (MnLGR2) was ide...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Cao X,Zhang C,Zhang R,Wang K,Dai X,Huang X,Ren Q

    更新日期:2020-10-01 00:00:00

  • An increase in the number of recombinant molecules and other effects of the simultaneous allotype suppression of trans-chromosomal a VH and n Cmu Ig gene products.

    abstract::The concomitant effects of trans-chromosomal allotype suppression of both an a VH and an n Cmu locus allotype in multiheterozygous rabbits were investigated. For example of the expression of the a2 VH and n81 Cmu allotypes were suppressed in a multiheterozygous rabbit having the a1 chi-y-n81de12,15f73g74/a2 chi 32y33n...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Horng WJ,Roux KH,Gilman-Sachs A,Dray S

    更新日期:1982-01-01 00:00:00

  • A phage antibody to the active site of human placental alkaline phosphatase with higher affinity to the enzyme-substrate complex.

    abstract::Selection of specific antibodies from large repertoires is of importance in generating antibodies to specific structural determinants and in studying structure-function relationships. Alkaline phosphatase (AP) has several isozymes with various degrees of homology and a range of common synthetic substrates. We have pre...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Jain V,Saini D,Goswami P,Sinha S

    更新日期:2007-01-01 00:00:00

  • Isolation and partial characterization of bovine and equine factor D.

    abstract::Bovine and equine factor D were purified to apparent homogeneity as evidenced by a single protein staining band on 7.5-17.5% SDS-PAGE slab gels under both reducing and non-reducing conditions. An apparent mol. wt of 15,000 for bovine D and 22,500 for equine D were noted after SDS-PAGE gel analysis of both reduced and ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Blanchard DB,Leid RW

    更新日期:1984-10-01 00:00:00

  • Characterization of the heavy and light chain immunoglobulin variable region genes used in a set of anti-digoxin antibodies.

    abstract::Five murine A/J hybridomas (the 35-20 group) produce anti-digoxin antibodies that have homologous heavy and light chain immunoglobulin variable regions (VH and VL), yet differ from each other in fine specificity and affinity for digoxin and related cardiac glycosides [Mudgett-Hunter et al. Molec. Immun. 22, 477-488 (1...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Near RI,Haber E

    更新日期:1989-04-01 00:00:00

  • NF-kappaB is essential for induction of pro-inflammatory cytokine genes by filarial parasitic sheath proteins.

    abstract::The transcription factor NF-kappaB plays critical roles in immune and inflammatory responses. Here we show that filarial parasitic sheath proteins cause activation of NF-kappaB in the airway epithelial HEp-2 cell line. This activation was transient and saturable, and involved degradation of the cytoplasmic inhibitor p...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Narayanan K,Balakrishnan A,Miyamoto S

    更新日期:2000-02-01 00:00:00

  • Toll-like receptor 3 (TLR3) protects retinal pigmented epithelium (RPE) cells from oxidative stress through a STAT3-dependent mechanism.

    abstract::Toll-like receptors (TLRs) are essential receptors of the innate immune system and are first responders for protection against bacterial and viral pathogens. Recently, several TLRs have also been implicated in regulating cell death and survival in non-pathogen injuries such as stroke and oxidative stress. Investigatin...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Patel AK,Hackam AS

    更新日期:2013-06-01 00:00:00

  • Novel monoclonal antibodies to cyclosporine A: characterization and epitope mapping with cyclosporine analogs and cyclophilin.

    abstract::Monoclonal antibodies to cyclosporine A (Cs), a potent immunosuppressant, were generated in BALB/c mice using a novel antigen prepared by linking Cs to a protein carrier via a photoactive cross-linking reagent, 4-benzoylbenzoic acid (BBa). Twenty-two monoclonal anti-Cs antibodies were generated, using Cs-BBa-bovine se...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Cacalano NA,Aggarwal R,Quesniaux VF,Cleveland WL,Erlanger BF

    更新日期:1992-01-01 00:00:00

  • MBL2, FCN1, FCN2 and FCN3-The genes behind the initiation of the lectin pathway of complement.

    abstract::Mannose-binding lectin (MBL) and the ficolins (Ficolin-1, Ficolin-2 and Ficolin-3) are soluble collagen-like proteins that are involved in innate immune defence. They bind sugar structures or acetylated compounds present on microorganisms and on dying host cells and they initiate activation of the lectin complement pa...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Garred P,Honoré C,Ma YJ,Munthe-Fog L,Hummelshøj T

    更新日期:2009-09-01 00:00:00

  • Activation of NF-κB induced by TRIMCyp showing a discrepancy between owl monkey and northern pig-tailed macaque.

    abstract::TRIMCyp generated by retrotransposition of a cyclophilin A inserting into TRIM5 locus, has been identified in owl monkey and most of Old World monkeys (OWM). Owl monkey TRIMCyp (omTRIMCyp) inhibits HIV-1 infection by direct interaction with viral capsid and indirect innate immune induction, whereas most of TRIMCyps fr...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Zhu JW,Mu D,Liu FL,Luo MT,Luo RH,Zheng YT

    更新日期:2018-09-01 00:00:00

  • MD-2 regulates LPS-induced NLRP3 inflammasome activation and IL-1beta secretion by a MyD88/NF-κB-dependent pathway in alveolar macrophages cell line.

    abstract::Myeloid differentiation protein 2 (MD-2) is required in the recognition of lipopolysaccharide (LPS) by toll-like receptor 4 (TLR4), and participates in LPS-induced alveolar macrophage (AM) inflammation during acute lung injury (ALI). Activation of the NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflamm...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Luo M,Hu L,Li D,Wang Y,He Y,Zhu L,Ren W

    更新日期:2017-10-01 00:00:00

  • Methotrexate regulates the expression of glucocorticoid receptor alpha and beta isoforms in normal human peripheral mononuclear cells and human lymphocyte cell lines in vitro.

    abstract::MTX is an effective therapy for autoimmune-inflammatory diseases. The mechanisms that mediate these actions are not completely clear. It is accepted that many of these effects are mediated through the release of adenosine with the activation of the adenosine receptor A2. MTX is used as a steroid sparing agent. An impr...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Goecke IA,Alvarez C,Henríquez J,Salas K,Molina ML,Ferreira A,Gatica H

    更新日期:2007-03-01 00:00:00

  • Differential requirement of PKC-theta in the development and function of natural regulatory T cells.

    abstract::CD4+CD25+ natural Treg cells, which are developed in the thymus, migrate to the periphery to actively maintain self-tolerance. Similar to conventional T cells, TCR signals are critical for the development and activation of Treg cell inhibitory function. While PKC-theta-mediated TCR signals are required for the activat...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Gupta S,Manicassamy S,Vasu C,Kumar A,Shang W,Sun Z

    更新日期:2008-12-01 00:00:00

  • Interleukin-1 Receptor-Associated Kinase-1 (IRAK-1) functionally associates with PKCepsilon and VASP in the regulation of macrophage migration.

    abstract::Macrophage migration is mediated by complex cellular signaling processes and cytoskeleton re-arrangement. In particular, recent advances indicate that the innate immunity signaling process plays a key role in the regulation of macrophage migration. In this report, we have provided evidence demonstrating the involvemen...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Gan L,Li L

    更新日期:2010-03-01 00:00:00

  • Comprehensive gene expression profiling following DNA vaccination of rainbow trout against infectious hematopoietic necrosis virus.

    abstract::The DNA vaccine based on the glycoprotein gene of Infectious hematopoietic necrosis virus induces a non-specific anti-viral immune response and long-term specific immunity against IHNV. This study characterized gene expression responses associated with the early anti-viral response. Homozygous rainbow trout were injec...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Purcell MK,Nichols KM,Winton JR,Kurath G,Thorgaard GH,Wheeler P,Hansen JD,Herwig RP,Park LK

    更新日期:2006-05-01 00:00:00

  • Junctional diversification in the generation of the precursor of a discrete immune response.

    abstract::Phosphocholine (PC)-specific antibodies that arise in the mouse in response to Proteus morganii (PM) and use V1-DFL16.1-JH1 are characterized by a number of recurring mutations. Most striking is an invariant A for G substitution in codon 95 of VH which results in an asparagine instead of aspartate at that position. Be...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: George J,Sheehan KM,Brodeur PH,Claflin JL

    更新日期:1993-03-01 00:00:00

  • Infectious salmon anaemia virus (ISAV) isolates induce distinct gene expression responses in the Atlantic salmon (Salmo salar) macrophage/dendritic-like cell line TO, assessed using genomic techniques.

    abstract::Infectious salmon anaemia virus (ISAV) is a marine orthomyxovirus of significant interest not only as a cause of a fatal disease of farmed Atlantic salmon resulting in severe economic losses to the aquaculture industry, but also as the only poikilothermic orthomyxovirus. ISAV targets vascular endothelial cells and mac...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Workenhe ST,Hori TS,Rise ML,Kibenge MJ,Kibenge FS

    更新日期:2009-09-01 00:00:00

  • Molecular basis of allergen cross-reactivity: non-specific lipid transfer proteins from wheat flour and peach fruit as models.

    abstract::Peach non-specific lipid transfer protein (Pru p 3; nsLTP) has been characterized as the major food allergen in the adult Mediterranean population. Its wheat homologous protein, Tri a 14 has a relevant inhalant allergen in occupational baker's asthma. Different sensitization patterns to these allergens have been found...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Tordesillas L,Pacios LF,Palacin A,Quirce S,Armentia A,Barber D,Salcedo G,Diaz-Perales A

    更新日期:2009-12-01 00:00:00

  • A novel dendritic cell-targeted lentiviral vector, encoding Ag85A-ESAT6 fusion gene of Mycobacterium tuberculosis, could elicit potent cell-mediated immune responses in mice.

    abstract::Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb), leading to high mortality worldwide. It is well-established that cellular immunity plays a critical role to control Mtb infection. Dendritic Cells (DCs) are potent antigen presenting cells, which play an important role to prime cell...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Shakouri M,Moazzeni SM,Ghanei M,Arashkia A,Etemadzadeh MH,Azadmanesh K

    更新日期:2016-07-01 00:00:00

  • Cytokine profile of CD4+CD25-FoxP3+ T cells in tumor-draining lymph nodes from patients with breast cancer.

    abstract:BACKGROUND:A T cell subtype with the CD4+CD25-FoxP3+ phenotype was recently described. We aimed to investigate the frequency of these cells and their ability to produce cytokines in tumor-draining lymph nodes from patients with breast cancer (BC). MATERIALS AND METHODS:Mononuclear cells from lymph nodes of 20 patients...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Niakan A,Faghih Z,Talei AR,Ghaderi A

    更新日期:2019-12-01 00:00:00

  • Characterization of circulating and monocyte-derived dendritic cells in obese and diabetic patients.

    abstract::Dendritic cells (DCs) are suspected to be involved in the development of atherogenesis, but their role is still unclear. The aim of this study was to characterize circulating DCs and monocyte-derived DCs (Mo-DCs) of obese and diabetic patients (T2D), and to study their interaction with human coronary smooth muscle cel...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Musilli C,Paccosi S,Pala L,Gerlini G,Ledda F,Mugelli A,Rotella CM,Parenti A

    更新日期:2011-10-01 00:00:00

  • Establishment of an ex vivo model of monocytes-derived macrophages differentiated from peripheral blood mononuclear cells (PBMCs) from HIV-1 transgenic rats.

    abstract::It has been developed an HIV type 1 transgenic rat model (HIV-1 Tg Rat) which contains a gag-pol-deleted HIV-1 provirus regulated by the viral LTR promoter. Although it harbors a non infectious provirus, efficient viral expression occurs in different tissues and disease manifestations as well as immune-response altera...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Mazzucchelli R,Amadio M,Curreli S,Denaro F,Bemis K,Reid W,Bryant J,Riva A,Galli M,Zella D

    更新日期:2004-08-01 00:00:00

  • Notch activation in thymic epithelial cells induces development of thymic microenvironments.

    abstract::The development and maintenance of thymic microenvironments depends on sustained crosstalk signals derived from developing thymocytes. However, the molecular basis for the initial phase in the lymphoid dependent development of thymic epithelial cells (TECs) remains unclear. Here we show that similarly to regular thymo...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Masuda K,Germeraad WT,Satoh R,Itoi M,Ikawa T,Minato N,Katsura Y,van Ewijk W,Kawamoto H

    更新日期:2009-05-01 00:00:00

  • Cloning, expression and characterization of mugwort pollen allergen Art v 2, a pathogenesis-related protein from family group 1.

    abstract::Mugwort (Artemisia vulgaris) belongs to the Compositae family, and is one of the main causes of allergy in late summer and autumn. The aim of the study was to characterize the allergen Art v 2 from mugwort pollen. Skin prick tests, performed in 19 patients allergic to mugwort and 10 control patients, showed an Art v 2...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Arilla MC,Ibarrola I,Puente Y,Daza JC,Martínez A,Asturias JA

    更新日期:2007-07-01 00:00:00

  • Non-enzymic activation of the fifth component of human complement, by oxygen radicals. Some properties of the activation product, C5b-like C5.

    abstract::Purified human C5 was converted non-enzymically to an activated form as defined by its ability to participate in reactive lysis. This conversion occurred following exposure to systems that generate oxygen radicals, namely addition of H2O2 in the presence of ascorbic acid and iron or the addition of xanthine oxidase, a...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Vogt W,Damerau B,von Zabern I,Nolte R,Brunahl D

    更新日期:1989-12-01 00:00:00

  • MKK6 from pacific white shrimp Litopenaeus vannamei is responsive to bacterial and WSSV infection.

    abstract::p38 mitogen-actived protein kinases (MAPKs) broadly exist from yeast to mammals and participate in diverse cellular responses to various stimuli, whose activation can be induced by the MAPK kinase 6 (MKK6). In this study, a novel MKK6 homolog from Litopenaeus vannamei (LvMKK6) was cloned and characterized. The transcr...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Li H,Wang S,Qian Z,Wu Z,Lǚ K,Weng S,He J,Li C

    更新日期:2016-02-01 00:00:00

  • Correlation between the human and porcine complement system: a small-angle scattering study of cross immunity and methylamine-induced conformational changes of porcine C3 and C4 proteins.

    abstract::The porcine complement proteins C3 and C4 have been isolated and then characterized using small-angle scattering methods. Within the limits of experimental errors, the porcine proteins are virtually identical with the corresponding human proteins as measured in terms of mol. wt, Mr and radius of gyration, R,: Mr(C3) =...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Osterberg R,Malmensten B,Boive T,Nilsson U,Stigbrand T,Mortensen K

    更新日期:1991-09-01 00:00:00

  • Molecular cloning and characterization of CD9 cDNA from cartilaginous fish, red stingray, Dasyatis akajei.

    abstract::CD9 is a glycoprotein of the transmembrane 4 superfamily (TM4SF) and is involved in various cellular processes. In this study, we describe the isolation of the full-length cDNA encoding for CD9 molecule (daCD9) of red stingray, Dasyatis akajei. This 1252 bp cDNA was isolated from leukocyte cDNA library and contains 68...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Zhu J,Yan K,Lu L,Peng C,Zhou C,Chen S,Xie X,Dong M,Xu A

    更新日期:2006-04-01 00:00:00