Abstract:
:REV1 plays a key role in vertebrate translesion synthesis. Although its deoxycytidyl transferase activity is dispensable for tolerance of DNA damage caused by a number of mutagens, its extreme C terminus, which interacts with other translesion polymerases and PCNA, is essential. By examining immunoglobulin diversification in the genetically tractable chicken cell line DT40 we show that the generation of non-templated point mutations from C/G to G/C does require the catalytic activity of REV1. This provides the first clear evidence that the catalytic activity of REV1 is utilised in vivo in higher eukaryotes and is involved in immunoglobulin diversification. Although rev1 DT40 cells incorporate few point mutations, a mutant lacking the C terminus of REV1 exhibits a similar level to that seen in wild-type cells. Thus, the polymerase selection or stabilisation role of REV1 does not appear to play a major role in the bypass of AID-dependent abasic sites.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Ross AL,Sale JEdoi
10.1016/j.molimm.2005.09.017subject
Has Abstractpub_date
2006-04-01 00:00:00pages
1587-94issue
10eissn
0161-5890issn
1872-9142pii
S0161-5890(05)00352-4journal_volume
43pub_type
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