Abstract:
:The role of glycosylation in membrane expression and secretion of IgM and IgA was investigated in murine lymphoma and hybridoma cell lines, derived from I.29 tumor, which synthesize IgM or IgA with identical variable regions. Tunicamycin, a selective inhibitor of N-linked glycosylation, prevented the membrane expression of both isotypes, as demonstrated by immunofluorescence, radioiodination and endogenous labeling experiments. Selective immunoprecipitation and immunochemical analysis of membrane, intracellular and secreted molecules permitted us to determine the amount of membrane heavy chain externalized in the presence or absence of tunicamycin. Id 150 and Id 43, two I.29-derived hybridomas secreting IgA and IgM respectively, were differently affected by tunicamycin. While secretion of IgM was inhibited to greater than 95%, no inhibition of secretion of non-glycosylated IgA could be detected in Id 150 cells. These results indicate that different requirements for glycosylation exist in the biosynthetic pathways of immunoglobulin isotypes, and suggest that distinct intracellular transport systems may operate for membrane and secreted alpha-chains.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Sitia R,Rubartelli A,Hämmerling Udoi
10.1016/0161-5890(84)90023-3subject
Has Abstractpub_date
1984-08-01 00:00:00pages
709-19issue
8eissn
0161-5890issn
1872-9142journal_volume
21pub_type
杂志文章abstract::Mice deficient for SPI-group ETS transcription factors PU.1 or SPI-B fail to generate lymphocytes or do not mount normal antibody mediated immune responses, respectively. PU.1 expression is restricted to B-, T-lymphocytes and macrophages, while SPI-B is expressed in B- and T-lymphocytes. SPI-C is an ETS transcription ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(03)00032-4
更新日期:2003-06-01 00:00:00
abstract::The transcription factor NF-kappaB plays critical roles in immune and inflammatory responses. Here we show that filarial parasitic sheath proteins cause activation of NF-kappaB in the airway epithelial HEp-2 cell line. This activation was transient and saturable, and involved degradation of the cytoplasmic inhibitor p...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(00)00039-0
更新日期:2000-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
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更新日期:2018-08-01 00:00:00
abstract::Emerging evidence has indicated that vascular endothelial cells (ECs) not only form the barrier between blood and the vessel wall but also serve as conditional innate immune cells. Our previous study found that SIRT1, a class III histone deacetylase, inhibits the inflammatory response in ECs. Recent studies revealed t...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.07.018
更新日期:2016-09-01 00:00:00
abstract::The fifth component of human complement (C5), under physiological salt conditions, bound firmly to phenyl-Sepharose. This indicated the presence of hydrophobic sites on C5. These sites may play an important role in C5 haemolytic activity since this activity was inhibited by chaotropic ions (Mg2+, SCN-, I-) at relative...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90031-4
更新日期:1988-04-01 00:00:00
abstract::Humanization of rodent mAbs by CDR-grafting (also called "reshaping") is now a standard procedure for reducing immunogenicity and recruiting human effector functions. However, the design of the humanized mAb can sometimes prove circuitous. Attempts were made to humanize L-25, a mouse antibody against the human alpha-4...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(99)00087-5
更新日期:1999-08-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.08.015
更新日期:2009-10-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(95)00059-n
更新日期:1995-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(00)00043-2
更新日期:2000-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.09.015
更新日期:2011-10-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.11.006
更新日期:2010-02-01 00:00:00
abstract::Antibodies that recognize Xenopus class II molecules have been developed. Mouse monoclonal antibodies were prepared by immunizing BALB/c mice with frog MHC antigens that had been partially purified with alloantisera, and by immunizing mouse spleen cells in vitro with activated Xenopus T lymphocytes. In addition, five ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(90)90170-5
更新日期:1990-05-01 00:00:00
abstract::This paper describes additional structural analyses of the p24 cell surface molecule recognized by monoclonal antibody BA-2. Since BA-2 is broadly reactive with a variety of normal and malignant lymphohematopoietic and nonlymphohematopoietic cells, we examined the structure of p24 expressed on different cell types. Tr...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90007-0
更新日期:1985-10-01 00:00:00
abstract::We investigated the structure and expression of immunoglobulin genes in the pufferfish, Takifugu rubripes, a highly prized and economically important fish species. The cDNA fragment that partially encodes the constant region of the IgM heavy chain was isolated in these animals by RACE using degenerate primers after wh...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.06.034
更新日期:2005-01-01 00:00:00
abstract::Crotoxin (Ctx) is the main lethal component of Crotalus durissus terrificus venom. It is a neurotoxin, composed of two subunits associated by noncovalent interactions, the non-toxic acid subunit (CA), named Crotapotin, and the basic subunit (CB), with phospholipase A2 (PLA2) activity. Employing the SPOT synthesis tech...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.01.012
更新日期:2020-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90118-0
更新日期:1987-12-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(86)90074-x
更新日期:1986-08-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.09.017
更新日期:2019-12-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.09.012
更新日期:2017-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90113-0
更新日期:1985-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(94)90068-x
更新日期:1994-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.10.013
更新日期:2016-12-01 00:00:00
abstract::Serotonin release from rat basophilic leukemia (RBL) cells, sensitized with a DNP-binding monoclonal IgE, was stimulated with solid surface (polystyrene)-bound DNP-amino acids. The stimulatory potency of DNP-amino acids was dependent on the structure of amino acid attached to DNP. Generally, DNP-amino acids with high ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(91)90134-6
更新日期:1991-06-01 00:00:00
abstract::Natural resistance to infection with intracellular parasites, such as Leishmania, Salmonella, and Mycobacterium, is controlled in mice by the expression of a single dominant gene locus designated Lsh/Ity/Bcg. Natural resistance-associated macrophage protein gene 1 (NRAMP1) was isolated as a candidate gene. NRAMP1 enco...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(96)00088-0
更新日期:1996-11-01 00:00:00
abstract::Two monoclonal antibodies, HH8 and HH9, have been established after immunization of mice with galactosyl-A glycolipid antigen having the terminal structure, Gal beta 1----3GalNAc alpha 1----3[Fuc alpha 1----2]Gal beta 1----R, which is the precursor for type 3 chain A (repetitive A) and type 3 chain H (A-associated H)....
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90068-5
更新日期:1988-02-01 00:00:00
abstract::The porcine complement proteins C3 and C4 have been isolated and then characterized using small-angle scattering methods. Within the limits of experimental errors, the porcine proteins are virtually identical with the corresponding human proteins as measured in terms of mol. wt, Mr and radius of gyration, R,: Mr(C3) =...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(91)90181-i
更新日期:1991-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.03.016
更新日期:2008-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.11.016
更新日期:2007-03-01 00:00:00
abstract:AIM:To study the role of complement receptor 1 (CR1) for binding of Escherichia coli (E. coli) to erythrocytes, for leukocyte phagocytosis, oxidative burst and complement activation in human whole blood from a CR1 deficient (CR1D) patient and healthy controls with low, medium and high CR1 numbers. METHODS:Alexa-labell...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.07.014
更新日期:2019-10-01 00:00:00
abstract::The MHC class II transactivator (CIITA) acts in the cell nucleus as the master regulator of MHC class II (MHC II) gene expression. It is important to study CIITA regulation in multiple myeloma since MHC expression is central to ability of myeloma cells to present antigen and to the ability of the immune system to reco...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.01.009
更新日期:2007-04-01 00:00:00