Abstract：
:The T cell receptor (TCR) detects the presence of infectious pathogens and activates numerous intracellular signaling pathways. Protein tyrosine phosphorylation and ubiquitination serve as key regulatory mechanisms downstream of the TCR. Negative regulation of TCR signaling pathways is important in controlling the immune response, and the Suppressor of TCR Signaling proteins (Sts-1 and Sts-2) have been shown to function as critical negative regulators of TCR signaling. Although their mechanism of action has yet to be fully uncovered, it is known that the Sts proteins possess intrinsic phosphatase activity. Here, we demonstrate that Sts-1 and Sts-2 are instrumental in down-modulating proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination. Specifically, both naïve and activated T cells derived from genetically engineered mice that lack the Sts proteins display strikingly elevated levels of tyrosine phosphorylated, ubiquitinated proteins following TCR stimulation. The accumulation of the dually modified proteins is transient, and in activated T cells but not naïve T cells is significantly enhanced by co-receptor engagement. Our observations hint at a novel regulatory mechanism downstream of the T cell receptor.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Carpino N,Chen Y,Nassar N,Oh HWdoi
10.1016/j.molimm.2009.08.015subject
Has Abstractpub_date
2009-10-01 00:00:00pages
3224-31issue
16eissn
0161-5890issn
1872-9142pii
S0161-5890(09)00679-8journal_volume
46pub_type
杂志文章abstract：:Major histocompatibility complex (MHC) class II molecules are cell surface glycoproteins and are known to display processed antigens on the surface of antigen presenting cells (APC). Within the APC, the loading of processed antigenic peptides to MHC class II molecules is known to take place in the endosomal compartmen...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00030-i
更新日期：1995-06-01 00:00:00
abstract：:Invertebrate cells lack the p53 recombination checkpoint but contain mobile DNA sequences that transpose by a mechanism in part shared with excision of the V(D)J recombination signal sequences (RSS). In this work, inversion, deletion, and duplication of sequences associated with an invertebrate C. elegans Tc6 element ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(99)00034-6
更新日期：1999-05-01 00:00:00
abstract：:Twelve C57BL/6J hybridoma clones, 9, 2 and 1 from mice immunized with stearyl-isomaltotetraose, stearyl-isomaltopentaose and stearyl-isomaltohexaose respectively were characterized. Seven produced IgA and 5 IgM. The specificities and sizes of their combining sites were determined by quantitative precipitin and precipi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90105-1
更新日期：1985-09-01 00:00:00
abstract：:Leprosy is a bacterial disease caused by M. leprae. Its clinical spectrum reflects the host's immune response to the M. leprae and provide an ideal model to investigate the host pathogen interaction and immunological dysregulation. Tregs are high in leprosy patients and responsible for immune suppression of the host b...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.01.008
更新日期：2017-03-01 00:00:00
abstract：:Twenty-seven murine monoclonal anti-hen egg-white lysozyme (HEL) antibodies were tested with a panel of nine antigens; eight avian lysozymes and human lysozyme. The antibodies were arranged into 10 groups based on their antigen specificity and cross-reactivity. Antigenic determinants recognized by each group of antibo...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90081-2
更新日期：1987-02-01 00:00:00
abstract：:In two recent articles published in Molecular Immunology, Steere and colleagues continue their search for an autoimmune mechanism of arthritis in patients who have failed short-course antibiotic therapy for Borrelia burgdorferi, the spirochetal agent of Lyme disease. As in previous attempts, the authors fail to elucid...
journal_title：Molecular immunology
pub_type： 评论,信件
doi：10.1016/j.molimm.2008.02.015
更新日期：2008-06-01 00:00:00
abstract：BACKGROUND:A T cell subtype with the CD4+CD25-FoxP3+ phenotype was recently described. We aimed to investigate the frequency of these cells and their ability to produce cytokines in tumor-draining lymph nodes from patients with breast cancer (BC). MATERIALS AND METHODS:Mononuclear cells from lymph nodes of 20 patients...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.10.007
更新日期：2019-12-01 00:00:00
abstract：:The alternative pathway of complement is receiving increasing attention as a therapeutic target because of recent findings in several animal models that support its essential role in tissue injury and disease pathogenesis. Although the contribution of alternative pathway activation to serum complement activation in vi...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2004.03.012
更新日期：2004-06-01 00:00:00
abstract：:T cell responses are determined by the environment in which antigen is encountered. In the absence of proper costimulation, anergizing stimuli induce the activation of a specific program of gene expression. Proteins encoded by these genes impose a state of functional unresponsiveness in anergic T cells through the act...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.09.029
更新日期：2009-02-01 00:00:00
abstract：:The Fc region of IgG antibodies is crucial for binding to Fc receptors expressed on the surfaces of various immune leukocytes and eliciting therapeutic effector functions such as clearance of antibody-opsonized tumor cells. Despite abrogated Fc gamma receptor (FcγR) binding and therapeutic effector function in the abs...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.07.007
更新日期：2019-10-01 00:00:00
abstract：:The mechanisms of each subset of immune cells contributing to the pathogenesis of viral hepatitis remain incompletely understood. In this study, we examined the role of liver CD4(-) CD8(-) (double negative, DN) T cells during murine hepatitis virus strain 3 (MHV-3)-induced hepatitis in C3H/HeJ mice. We demonstrate tha...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.05.017
更新日期：2012-10-01 00:00:00
abstract：:The high affinity receptor for IgE (FcvarepsilonRI) is constitutivelly expressed on the surface of mast cells and basophils as a multimeric complex. Upon antigen ligation to FcvarepsilonRI-bound IgE molecules, the receptor complex transduces intracellular signals leading to the release of preformed and newly synthesis...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2010.06.003
更新日期：2010-09-01 00:00:00
abstract：:B cells play a critical role in immune responses both in physiological and pathological conditions, and microRNAs have been shown to play important roles in regulating B cell proliferation and function. MiR-146a has been shown to modulate T cell immunity, but its function in regulating B cell response remains partiall...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.10.023
更新日期：2017-12-01 00:00:00
abstract：:Despite the fact that receptors for complement on T-cells have been described many years ago the function remains unclear as is the role of complement in the T-cell response. In this review we will evaluate how the accumulated wisdom concur with the current concepts of the adaptive T-cell response. ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2005.06.027
更新日期：2006-01-01 00:00:00
abstract：:Membrane immunoglobulins are integral proteins on B cell surfaces that bind foreign antigens and are critically involved in the regulation of the immune response. Based upon the model of serum IgG, it has been assumed that membrane immunoglobulins are essentially four chain disulfide-linked structures of the form H2L2...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90451-g
更新日期：1993-12-01 00:00:00
abstract：:Four different proteases were screened for their capability of selectively digesting murine monoclonal IgGl to obtain active F(ab)2. For the screening, a series of five different mouse monoclonal antibodies (IgGl, k) was used, recognizing different tumor-associated antigens and currently used for radioimmunoimaging st...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90088-2
更新日期：1991-01-01 00:00:00
abstract：:The peptide editor HLA-DM (DM) catalyzes the exchange of peptides bound to MHC class II molecules within antigen presenting cells by generating a "peptide-receptive" MHC class II conformation (MHC(receptive)) to which peptides readily bind and rapidly unbind. While recent work has uncovered the determinants of DM reco...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.07.001
更新日期：2009-09-01 00:00:00
abstract：:The discovery of a human myeloma protein comprising a kappa L-chain with an increased mol. wt of 30,000) (Bouvet et. al., 1980) prompted investigations on the incidence of such heavier L-chains among other human myeloma proteins. In 105 samples examined, 34 were found to have L-chains heavier than normal (23,000-24,00...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90021-4
更新日期：1983-04-01 00:00:00
abstract：AIM:To study the role of complement receptor 1 (CR1) for binding of Escherichia coli (E. coli) to erythrocytes, for leukocyte phagocytosis, oxidative burst and complement activation in human whole blood from a CR1 deficient (CR1D) patient and healthy controls with low, medium and high CR1 numbers. METHODS:Alexa-labell...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.07.014
更新日期：2019-10-01 00:00:00
abstract：:Candida albicans is an opportunistic human-pathogenic fungus, which can cause superficial but also life-threatening invasive infections. The pH-regulated antigen 1 (Pra1) of C. albicans is a surface-associated and secreted protein highly expressed in the hyphal form. Pra1 can bind to complement receptor 3 (CD11b/CD18)...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.07.007
更新日期：2011-09-01 00:00:00
abstract：:The ragweed allergens Amb t 5 and Amb a 5 are among the smallest inhaled protein allergens known, containing a single, immunodominant T-cell epitope. In this study we analyzed the B-cell epitope structure of Amb t 5. The three-dimensional structures of Amb t 5 and Amb a 5 have been determined by NMR spectroscopy, prov...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(99)00054-1
更新日期：1998-06-01 00:00:00
abstract：:In the present study, we show that the treatment of Epstein-Barr virus (EBV) latently infected Raji cells with TPA/SB caused the cell growth arrest. The Zta-positive cells were predominantly enriched in G0/G1 phase of cell cycle. When Zta expression reached a maximal level, a fraction of Zta expressing cell population...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.02.017
更新日期：2010-05-01 00:00:00
abstract：:IL-10 plays a critical role in controlling inflammation and the anti-inflammatory functions of IL-10 are regulated based on its coordinated expression from various cellular sources, most notably T cells. Although nearly all CD4+ subpopulations can express IL-10, surprisingly little is known about the molecular mechani...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.09.009
更新日期：2010-11-01 00:00:00
abstract：:Recent studies have shown that aberrant activation of the complement system plays an important role in the pathogenesis of preeclampsia. There is evidence to suggest that aberrant activation of the complement system may already be present during the first trimester. Here, we performed a prospective study in which peri...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.03.021
更新日期：2020-04-16 00:00:00
abstract：:Deficiencies in the classical pathway of complement activation have some common features but show also great differences. Deficiencies of each of the components (C1q, C1s, C1r, C4 and C2) imply increased susceptibility to bacterial infections. They are also associated with increased risk to develop systemic lupus eryt...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2015.05.007
更新日期：2015-11-01 00:00:00
abstract：:The developmental regulation of antigen receptor gene transcription and recombination are mediated by cis regulatory elements. At the T cell receptor beta chain locus (TCRbeta), two DNase I hypersensitive sites within the Jbeta2-Cbeta2 intron contained binding sites for NF-kappaB and additional nuclear factors and wer...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(01)00031-1
更新日期：2001-01-01 00:00:00
abstract：:An attempt was made to obtain fragments containing the C gamma 2 region by selectively cleaving human Fc fragments prepared from pooled IgG at Met residues using cyanogen bromide. Based on the known locations of Met residues in the Fc regions of human IgG subclasses, fragments between Met 252 and 358, comprising the C...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90052-5
更新日期：1985-12-01 00:00:00
abstract：:We have designed a 39 amino acid peptide mimic of the conformation-dependent main immunogenic region (MIR) of the Torpedo acetylcholine receptor (TAChR) that joins three discontinuous segments of the Torpedo α-subunit, α(1-12), α(65-79), and α(110 - 115) with two GS linkers: This 39MIR-mimic was expressed in E. coli a...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.01.002
更新日期：2014-05-01 00:00:00
abstract：BACKGROUND:Ethanol feeding in mice activates complement via C1q binding to apoptotic cells in the liver; complement contributes to ethanol-induced inflammation and injury. Despite the critical role of C1q in ethanol-induced injury, the mechanism by which ethanol activates C1q remains poorly understood. Secretory IgM (s...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.02.008
更新日期：2016-04-01 00:00:00
abstract：:Regarding the role of micro RNAs (miRNA) in the proliferation and differentiation of T cells as well as the controversy around the role of bacteria in the pathogenesis of abdominal aortic aneurysm (AAA), the effects of Helicobacter pylori (Hp) and Lactobacillus acidophilus (La) were investigated in the induction of mi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.11.007
更新日期：2021-02-01 00:00:00
