Abstract：
:In the present study, we show that the treatment of Epstein-Barr virus (EBV) latently infected Raji cells with TPA/SB caused the cell growth arrest. The Zta-positive cells were predominantly enriched in G0/G1 phase of cell cycle. When Zta expression reached a maximal level, a fraction of Zta expressing cell population reentered S phase. Analysis of the expression pattern of a key set of cell cycle regulators revealed that the expression of Zta and Rta substantially interfered with the cell cycle regulatory machinery in Raji cells, strongly inhibiting the expression of Rb and p53 and inducing the expression of E2F1. Down-regulation of Rb was further demonstrated to be mediated by proteasomal degradation, and p53 and p21 affected at transcription level. The data indicate that both Zta and Rta promote entry into S phase of Raji cells. The important roles of Zta and Rta in EBV lytic reactivation were also demonstrated. Our finding suggests that these two transcriptional activators may act synergistically to govern the expression of downstream early and late genes as well as cellular genes and initiation of lytic cycle and manipulation of cell cycle regulatory mechanisms require the joint and interactive contributions of Rta and Zta.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Guo Q,Qian L,Guo L,Shi M,Chen C,Lv X,Yu M,Hu M,Jiang G,Guo Ndoi
10.1016/j.molimm.2010.02.017subject
Has Abstractpub_date
2010-05-01 00:00:00pages
1783-92issue
9eissn
0161-5890issn
1872-9142pii
S0161-5890(10)00067-2journal_volume
47pub_type
杂志文章abstract：:Antibodies that recognize Xenopus class II molecules have been developed. Mouse monoclonal antibodies were prepared by immunizing BALB/c mice with frog MHC antigens that had been partially purified with alloantisera, and by immunizing mouse spleen cells in vitro with activated Xenopus T lymphocytes. In addition, five ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90170-5
更新日期：1990-05-01 00:00:00
abstract：:Clonal idiotypic immunoglobulins of follicular lymphomas can be isolated by somatic fusion procedures. Idiotypic IgMs (Id-IgM) were isolated from two patients and used to immunise a strain of mice, deficient in mouse antibody production and engineered with yeast artificial chromosomes (YAC) containing fragments of the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.03.035
更新日期：2004-07-01 00:00:00
abstract：:The A/J murine hybridoma cell line 40-150 secretes antidigoxin antibodies with high affinity for digoxin. A first-order spontaneous mutant (40-150 A2.4) produces antibodies containing a mutation at heavy chain position 94 resulting in reduced affinity for digoxin. A second-order mutant (40-150 A2.4 P.10) derived from ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90010-u
更新日期：1992-04-01 00:00:00
abstract：:Mannan-binding lectin (MBL) is a plasma protein of the innate immune system with the ability to initiate antimicrobial and inflammatory actions. MBL deficiency is common. More than 10% of the general population may, depending on definition, be classified as MBL deficient, underlining the redundancy of the immune syste...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2005.06.018
更新日期：2006-01-01 00:00:00
abstract：:The complete cDNA sequences of two bovine IgGs were obtained by RT-PCR cloning. The first-strand cDNA was prepared from an animal homozygous (A2/A2) for IgG2a; the resulting sequences of the two bovine IgGs reported here were identified as IgG2a(A2) and IgG1. These sequences, and their deduced amino acid sequences, ar...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00107-7
更新日期：1996-02-01 00:00:00
abstract：:Peptide fragments of pertussis toxin subunit 1 (PT-S1) have been synthesized in order to investigate their antigenic and immunogenic activity, and to evaluate their possible use as components of a new vaccine. Two peptides (sequence 73-82, EAERAGRGTG and sequence 107-116, YVDTYGDNAG) were selected for their predictabl...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90025-4
更新日期：1989-01-01 00:00:00
abstract：:Pulmonary surfactant proteins, SP-A and SP-D, are carbohydrate pattern recognition molecules of innate immunity, which significantly enhance phagocytosis and killing of Aspergillus fumigatus, a pathogenic fungus, by neutrophils and macrophages. The present study examined the susceptibility of immunosuppressed SP-A gen...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.02.027
更新日期：2010-06-01 00:00:00
abstract：:The expression levels of three commonly used housekeeping genes, EF1-alpha, RPS20 and Beta-Actin, were examined in seven different tissues and leucocytes from non-stimulated Atlantic salmon (Salmo salar L.). The tissues analysed by quantitative real-time PCR were gill, liver, intestine, muscle, spleen, head kidney leu...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.07.009
更新日期：2006-03-01 00:00:00
abstract：:Attempts to develop peptide vaccines, based on a limited number of peptides face two problems: HLA polymorphism and the high mutation rate of viral epitopes. We have developed a new genomic method that ensures maximal coverage and thus maximal applicability of the peptide vaccine. The same method also promises a large...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.06.003
更新日期：2007-02-01 00:00:00
abstract：:An antimicrobial polypeptide was purified from an acidified gill extract of Pacific oyster (Crassostrea gigas) by C(18) reversed-phase HPLC. The purified polypeptide had a molecular weight of 8471Da containing 74 amino acid residues. Comparison of the obtained N-terminal sequences with those of others revealed that it...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.07.003
更新日期：2013-01-01 00:00:00
abstract：:The S100A12 gene belongs to the S100 family of genes, which are specific to vertebrates. It is involved in many inflammatory diseases of human and has been considered as a powerful diagnostic gene. In the present study, we identified the porcine S100A12 (pS100A12) gene, provided evidence that pS100A12 is located on ch...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.01.024
更新日期：2010-04-01 00:00:00
abstract：:The effect of fragment B of staphylococcal protein A on the binding of rabbit IgG to human granulocytes has been examined. When rabbit IgG sensitized sheep erythrocytes (EA) were preincubated with increasing amounts of fragment B, a dose dependent inhibition of rosette formation between the human granulocytes and the ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90206-1
更新日期：1982-03-01 00:00:00
abstract：:Since secretion of IgE antibodies is known to be blocked by tunicamycin, the first aim of the present study was to determine at which step of glycosylation or processing secretion was restored. For this purpose, murine hybridoma cells secreting an anti beta-lactoglobulin IgE were incubated either in the presence of in...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90187-8
更新日期：1987-08-01 00:00:00
abstract：:Members of the genus Ebolavirus cause fulminating outbreaks of disease in human and non-human primate populations with a mortality rate up to 90%. To facilitate rapid detection of these pathogens in clinical and environmental samples, robust reagents capable of providing sensitive and specific detection are required. ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.06.437
更新日期：2011-09-01 00:00:00
abstract：:ASF1a (anti-silencing function 1a), an evolutionarily conserved protein and a histone chaperone, is required for a variety of chromatin-mediated cellular processes. However, the function of ASF1a in innate immune response remains unclear. Here, we find that ASF1a is induced in Vesicular Stomatitis Virus (VSV)-infected...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.08.008
更新日期：2016-10-01 00:00:00
abstract：:the SC5b-8 complex of human complement is a hydrophilic molecule of mol. wt 800,000-850,000 that is seen in the electron microscope as an elongated, straight or curved structure of 50-55 nm total length and 8-9 nm width. Tryptic attack on the fluid-phase complex exposes lipid-binding surfaces on the molecule. The tryp...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90327-3
更新日期：1982-09-01 00:00:00
abstract：:The myobacterial factors and the associated mechanism by which Mycobacterium tuberculosis (Mtb) evades the host immune surveillance system remain widely unexplored. Here, we found that overexpressing Rv1016c, a mannosylated protein of M. tuberculosis in BCG (rBCG-Rv1016c) led to increased virulence of the recombined B...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.02.021
更新日期：2019-05-01 00:00:00
abstract：:The fifth component of human complement (C5), under physiological salt conditions, bound firmly to phenyl-Sepharose. This indicated the presence of hydrophobic sites on C5. These sites may play an important role in C5 haemolytic activity since this activity was inhibited by chaotropic ions (Mg2+, SCN-, I-) at relative...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90031-4
更新日期：1988-04-01 00:00:00
abstract：:Two recombinant mouse mammary tumor virus (MMTV) subunit vaccines have been constructed, in which linear sequences of the envelope gp 52 glycoprotein (EP3) or the superantigen (SAg) have been fused to single or multiple repeats of a T-cell epitope (P30) from tetanus toxin. Histidine tags or glutathione-S-transferase (...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(96)00068-5
更新日期：1996-09-01 00:00:00
abstract：:Gene regulatory network (GRN) subcircuits have been described for cell fate progressions in animal development. The hallmark of these subcircuits is the integration of promoters, and positive- and negative-acting promoter binding proteins, such that an alteration in function of any one member of the defined subcircuit...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.07.035
更新日期：2009-02-01 00:00:00
abstract：:We designed the peptide (C3) mimetic carboxyl-terminus (Cterminus) of capsid protein of porcine circovirus type 2b (PCV2b-1A/1B) inducing humoral immunity and generating hybridomas. The positive reactivity of the mAbs to PCV2 capsid protein was demonstrated by Western blot assay. Those mAbs also showed positive signal...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.02.001
更新日期：2017-05-01 00:00:00
abstract：:The B1 molecule (CD20) is a phosphoprotein expressed only by B-lymphocytes. In this study, analysis of B1 immunoprecipitated from surface iodinated B-cell lines and B-lymphocytes has shown that there are several expressed forms of B1. A predominant species of Mr 33,000 represents 75-80% of the iodinated cell surface B...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90047-8
更新日期：1988-12-01 00:00:00
abstract：:Recent reports demonstrate that natural killer (NK) cells and dendritic cells (DC) support each other's activity in a positive feedback. We observed that activated NK cells induce the maturation of DCs into stable type-1 polarized DCs (DC1), characterized by up to 100-fold enhanced ability to produce IL-12p70 in respo...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2004.07.038
更新日期：2005-02-01 00:00:00
abstract：:The cancer immunosurveillance hypothesis has found strong experimental support in recent years. It is believed that cytotoxic lymphocytes are important effectors in this process. PKCtheta plays an essential role in proliferation, activation and survival of these cells, but also proliferation and survival of leukemic T...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.03.016
更新日期：2008-07-01 00:00:00
abstract：:We have previously shown that immunization with SIV-, SHIV-, or HA (influenza hemagglutinin)-virus-like particles (VLPs) elicits a strong humoral immune response in mice. However, little is known about the action VLPs exert on immune effector cells, including B cells. In this study, we found that all three types of VL...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.03.008
更新日期：2009-06-01 00:00:00
abstract：INTRODUCTION:Previous studies have suggested the importance of somatic mutations and arginine, asparagine and lysine residues in the complementarity determining regions (CDRs) of antiphospholipid antibodies (aPL) implicated in the pathogenesis of the antiphospholipid antibody syndrome. The relative contributions of the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(03)00100-7
更新日期：2003-09-01 00:00:00
abstract：:The T cell stimulatory activity of peptides is known to be associated with the cell surface stability and lifetime of the peptide-MHC (pepMHC) complex. In this report, soluble high-affinity T cell receptors (TCRs) that are specific for pepMHC complexes recognized by the mouse CD8+ clone 2C were used to monitor the cel...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.11.016
更新日期：2007-03-01 00:00:00
abstract：BACKGROUND:Smalotrophomonas maltophilia(S. maltophilia) is common in nosocomial infections. However, few studies have revealed the effect of S. maltophilia on cellular immunity in the host's immune system up to now. In clinical work, we accidentally discovered that S. maltophilia directly stimulated T cells to secrete ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.12.019
更新日期：2021-02-01 00:00:00
abstract：:HCA2 is a widely used monoclonal antibody, thought to be highly selective for HLA-A and -G heavy chains. We demonstrate here that it also shows affinity to HLA-B73 and HLA-E molecules on intact cells. By comparing the differences in the amino acid (AA) sequences of several HLA class I alleles that are either recognise...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00077-7
更新日期：1998-09-01 00:00:00
abstract：:Mycobacterium tuberculosis (M.tb) infects 8 million and kills 2.2 million people each year worldwide. M.tb modulates the immune response of the infected individual. Empirically, suppressor carbohydrates (SC) produced by CD8+ T cells in response to M.tb were found to induce a T helper 2 response rather than a protectiv...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.10.022
更新日期：2008-03-01 00:00:00
