Abstract:
:Two recombinant mouse mammary tumor virus (MMTV) subunit vaccines have been constructed, in which linear sequences of the envelope gp 52 glycoprotein (EP3) or the superantigen (SAg) have been fused to single or multiple repeats of a T-cell epitope (P30) from tetanus toxin. Histidine tags or glutathione-S-transferase (GST) sequences have been included in the recombinant peptides in order to facilitate their purification by affinity chromatography. The EP3 or SAg recombinant polypeptides with four, one, or no T-cell epitopes were expressed in Escherichia coli, purified and injected intramuscularly, with non-ionic block copolymers as an adjuvant, into BALB/c mice. Following one or two boosts, the B- and T-cell responses against the recombinant proteins were analysed. The addition of T-cell epitopes considerably increased the immunogenicity of the subunit vaccines. The anti-EP3 response was maximum with four T-cell epitopes, while a single T epitope was optimal for the anti-SAg response.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Astori M,Kraehenbuhl JPdoi
10.1016/s0161-5890(96)00068-5subject
Has Abstractpub_date
1996-09-01 00:00:00pages
1017-24issue
13eissn
0161-5890issn
1872-9142pii
S0161589096000685journal_volume
33pub_type
杂志文章abstract::The S100A12 gene belongs to the S100 family of genes, which are specific to vertebrates. It is involved in many inflammatory diseases of human and has been considered as a powerful diagnostic gene. In the present study, we identified the porcine S100A12 (pS100A12) gene, provided evidence that pS100A12 is located on ch...
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