Abstract：
:Very sensitive assays of IgE are required for determining prevalence of allergic reactions in children. In order to develop a sensitive two-site IRMA two kinds of mAb were produced. Antibodies specific for D epsilon 1 determinants were derived from immunization with a 40 kDa papain Fc fragment. They bound equally native and 56 degrees C heated IgE. D epsilon 2 specific mAb were obtained after immunization with IgE anti-D epsilon 1 complex and were selected on the basis of their inability to bind heated IgE. In a two-site assay on plastic plates, D epsilon 1 specific mAb led to the binding of IgE but always prevented further binding of anti-D epsilon 1 mAb, anti-human kappa chain mAb or allergen on bound IgE. This was not true when CNBr activated cellulose was used. The influence of the nature of the solid phase disappeared when D epsilon 2 specific mAb were coated on plastic tubes. In this case, the binding of a second mAb with identical or different fine specificity was observed. The best matched pair was E 164 (anti-D epsilon 2) on the solid phase and 6H10 (anti-D epsilon 1) as a tracer. As little as 0.2 UI/ml of IgE could be detected in a 2 hr test.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Anfosso F,Demeure C,Delaage M,Cheballah R,Bellot F,Bourgois Adoi
10.1016/0161-5890(87)90158-1subject
Has Abstractpub_date
1987-11-01 00:00:00pages
1129-34issue
11eissn
0161-5890issn
1872-9142journal_volume
24pub_type
杂志文章abstract：:Increasing evidence suggests that inflammation and apoptosis are involved in the development of acute myocardial ischemia (AMI). Nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) have recently been identified as key mediators of inflammatory responses. The aim of this study was to explore the speci...
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更新日期：2008-08-01 00:00:00
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journal_title：Molecular immunology
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更新日期：2005-06-01 00:00:00
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journal_title：Molecular immunology
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journal_title：Molecular immunology
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journal_title：Molecular immunology
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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更新日期：1999-09-01 00:00:00
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