当前位置： SCI文献检索 > MOLECULAR IMMUNOLOGY期刊下所有文献 > Integrin CD11c contributes to monocyte adhesion with CD11b in a differential manner and requires Src family kinase activity.

Integrin CD11c contributes to monocyte adhesion with CD11b in a differential manner and requires Src family kinase activity.

Abstract：

:Integrin-mediated adhesion of human monocytes to fibrinogen regulated by CD11b/CD18 and the closely related integrin CD11c/CD18, play a key role in inflammation. Peripheral blood monocytes isolated from human donors despite expressing CD11c primarily utilized CD11b to mediate adhesion to fibrinogen upon stimulation with granulocyte macrophage-colony stimulating factor (GM-CSF) and fMLP. Blocking with anti-CD11b resulted in 90% (p<0.001, n=3) inhibition of monocyte adhesion. Monocytes cultured in human serum showed a shift in the participation of integrins, adhesion to fibrinogen involving both CD11b and CD11c. The participation of CD11c in cultured monocytes corresponded to a 3.4-fold increase in expression in CD11c. Blocking cultured monocytes with anti-CD11b or anti-CD11c alone showed no significant effect on adhesion. Treatment with both anti-CD11b and anti-CD11c resulted in inhibition of adhesion by 85% (p<0.001, n=3). Abrogation in adhesion upon treatment with PP1 or PP2 showed that Src family kinase activity was required for CD11b and CD11c mediated adhesion of cultured monocytes to fibrinogen upon stimulation with GM-CSF and fMLP. The clustering of CD11c on cultured monocytes upon adhesion to fibrinogen was diminished on inhibition with PP2 indicating a role for Src family kinase activity in regulating CD11c avidity. CD11b was critical to cytoskeletal events leading to increased spreading and formation of actin foci in cultured monocytes following adhesion to fibrinogen. Blocking cultured monocytes with anti-CD11b or anti-CD11c alone showed that the increase in spread area was diminished by 67+/-3% and 36+/-9%, respectively. The differential involvement of CD11c and CD11b in adhesion and subsequent cytoskeletal changes in monocytes exposed to different conditions indicates the importance of each integrin in distinct responses during inflammation.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Georgakopoulos T,Moss ST,Kanagasundaram V

doi

10.1016/j.molimm.2008.04.021

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

3671-81

issue

13

eissn

0161-5890

issn

1872-9142

pii

S0161-5890(08)00162-4

journal_volume

45

pub_type

杂志文章

相关文献

MOLECULAR IMMUNOLOGY文献大全
  • Characterization of the heavy and light chain immunoglobulin variable region genes used in a set of anti-digoxin antibodies.

    abstract：:Five murine A/J hybridomas (the 35-20 group) produce anti-digoxin antibodies that have homologous heavy and light chain immunoglobulin variable regions (VH and VL), yet differ from each other in fine specificity and affinity for digoxin and related cardiac glycosides [Mudgett-Hunter et al. Molec. Immun. 22, 477-488 (1...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(89)90126-0

    authors： Near RI,Haber E

    更新日期：1989-04-01 00:00:00

  • Inflammatory pathways in spondyloarthritis.

    abstract：:Spondyloarthritis is the second most common form of chronic inflammatory arthritis and a unique hallmark of the disease is pathologic new bone formation. Several cytokine pathways have been genetically associated with ankylosing spondylitis (AS), the prototypic subtype of SpA, and additional evidence from human and an...

    journal_title：Molecular immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molimm.2013.07.016

    authors： Hreggvidsdottir HS,Noordenbos T,Baeten DL

    更新日期：2014-01-01 00:00:00

  • Isolation and structure-functional characterization of phage display library-derived mimotopes of noxiustoxin, a neurotoxin of the scorpion Centruroides noxius Hoffmann.

    abstract：:Noxiustoxin (NTX) is a short-chain toxin from the venom of the scorpion Centruroides noxius Hoffmann, whose molecular structure and physiological effects have been characterized in detail, whereas the antigenic properties of this and other K(+) channel-blocking toxins are poorly studied. A monoclonal antibody against ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/s0161-5890(00)00091-2

    authors： Gazarian T,Selisko B,Hérion P,Gazarian K

    更新日期：2000-08-01 00:00:00

  • The human mast cell receptor binding site maps to the third constant domain of immunoglobulin E.

    abstract：:The characterization of the site on the IgE molecule which accommodates the high affinity receptor for IgE (Fc epsilon RI) should allow the design of IgE analogues which can be utilized to block allergic responses. Using chimeric human IgE molecules in which different constant region domains were exchanged with their ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(92)90038-y

    authors： Nissim A,Eshhar Z

    更新日期：1992-09-01 00:00:00

  • Chemokine-mediated inflammation: Identification of a possible regulatory role for CCR2.

    abstract：:The chemokine receptor CCR2 binds four pro-inflammatory monocyte chemoattractant proteins, designated MCP1/CCL2, MCP2/CCL8, MCP3/CCL7 and MCP4/CCL13. This study demonstrates the important biology of this receptor during the response to the chemokine milieu. Competitive chemotaxis and calcium flux assays were performed...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2006.09.033

    authors： O'Boyle G,Brain JG,Kirby JA,Ali S

    更新日期：2007-03-01 00:00:00

  • Characterization of an anti-Bla g 1 scFv: epitope mapping and cross-reactivity.

    abstract：:Bla g 1 is a major allergen from Blatella germanica and one of the primary allergens used to assess cockroach allergen exposure. The epitope of an anti-Bla g 1 scFv was mapped in order to better understand cross reactivity with other group 1 cockroach allergens and patient IgE epitopes. X-ray crystallography was used ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2014.02.003

    authors： Mueller GA,Ankney JA,Glesner J,Khurana T,Edwards LL,Pedersen LC,Perera L,Slater JE,Pomés A,London RE

    更新日期：2014-06-01 00:00:00

  • MicroRNA-146a promotes IgE class switch in B cells via upregulating 14-3-3σ expression.

    abstract：:B cells play a critical role in immune responses both in physiological and pathological conditions, and microRNAs have been shown to play important roles in regulating B cell proliferation and function. MiR-146a has been shown to modulate T cell immunity, but its function in regulating B cell response remains partiall...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2017.10.023

    authors： Li F,Huang Y,Huang YY,Kuang YS,Wei YJ,Xiang L,Zhang XJ,Jia ZC,Jiang S,Li JY,Wan Y

    更新日期：2017-12-01 00:00:00

  • The catfish liver-expressed antimicrobial peptide 2 (LEAP-2) gene is expressed in a wide range of tissues and developmentally regulated.

    abstract：:Antimicrobial peptides (AMPs) are important components of the host's innate immune response against microbial invasion. The cysteine-rich AMPs such as defensin and hepcidin have been extensively studied, but the recently identified cysteine-rich liver-expressed antimicrobial peptide 2 (LEAP-2) has been characterized f...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2005.02.014

    authors： Bao B,Peatman E,Xu P,Li P,Zeng H,He C,Liu Z

    更新日期：2006-02-01 00:00:00

  • Th17 activation by dendritic cells stimulated with gamma-irradiated Streptococcus pneumoniae.

    abstract：:Dendritic cells (DCs) play an important role in antigen presentation, which is an essential step for the induction of antigen-specific adaptive immunity. Inactivated bacterial whole cell vaccines have been widely used to prevent many bacterial infections because they elicit good immunogenicity due to the presence of v...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2018.07.023

    authors： Kim HY,Kim SK,Seo HS,Jeong S,Ahn KB,Yun CH,Han SH

    更新日期：2018-09-01 00:00:00

  • An afucosylated anti-CD20 monoclonal antibody with greater antibody-dependent cellular cytotoxicity and B-cell depletion and lower complement-dependent cytotoxicity than rituximab.

    abstract：:The objective of this study was to characterize the in vitro and in vivo activity of a novel afucosylated rituximab (BLX-300) expressed in a Lemna aquatic plant-based system free of zoonotic pathogens. The glycosylation of BLX-300 was shown to be homogeneous, composed of a single major N-glycan species without detecta...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2012.01.001

    authors： Gasdaska JR,Sherwood S,Regan JT,Dickey LF

    更新日期：2012-03-01 00:00:00

  • Fine molecular analysis of the antigenicity of the Androctonus australis hector scorpion neurotoxin II: a new antigenic epitope disclosed by the Pepscan method.

    abstract：:A set of 58 overlapping rod-bound peptides was used to map the antigenic reactivity pattern of a 64-residue neurotoxin (AaH II) from the venom of the scorpion Androctonus australis hector. Five anti-toxin rabbit antisera were assayed serially for their capacity to bind to each peptide in the set. Six regions of antige...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(93)90152-2

    authors： Devaux C,Juin M,Mansuelle P,Granier C

    更新日期：1993-08-01 00:00:00

  • Anti-human CD63 monoclonal antibody COS3A upregulates monocyte-induced IL-10 excretion leading to diminution of CD3-mediated T cell response.

    abstract：:Human CD63 has been reported to play a role either as an inhibitor or as a co-stimulator of T- cell responses, although the mechanism of this is unclear. In this study, an anti-human CD63 monoclonal antibody (mAb) COS3A was used to monitor the role of CD63 in T-cell activation. MAb COS3A could inhibit CD3-mediated T-c...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2019.09.005

    authors： Wansook S,Mahasongkram K,Chruekamlow N,Pata S,Kasinrerk W,Khunkaewla P

    更新日期：2019-10-01 00:00:00

  • Non-traditional roles of complement in type 2 diabetes: Metabolism, insulin secretion and homeostasis.

    abstract：:Type 2 Diabetes (T2D) is a disease of increasing importance and represents a growing burden on global healthcare and human health. In T2D, loss of effectiveness of insulin signaling in peripheral tissues cannot be compensated for by adequate insulin secretion, leading to hyperglycemia and resultant complications. In r...

    journal_title：Molecular immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molimm.2016.12.009

    authors： King BC,Blom AM

    更新日期：2017-04-01 00:00:00

  • PAX2 oncogene negatively regulates the expression of the host defense peptide human beta defensin-1 in prostate cancer.

    abstract：:Human beta defensin-1 (hBD1) is a component of the immune system which links the innate and adaptive immune responses. We have demonstrated that hBD1 induces rapid cytolysis of prostate cancer cells and that it may also possess tumor suppressive abilities. In addition, there is a high frequency of cancer-specific loss...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2008.11.004

    authors： Bose SK,Gibson W,Bullard RS,Donald CD

    更新日期：2009-03-01 00:00:00

  • The TNFα converting enzyme (TACE) from ayu (Plecoglossus altivelis) exhibits TNFα shedding activity.

    abstract：:TNFα converting enzyme (TACE) is responsible for converting membrane-anchored TNFα to its soluble form in mammalian. However, the function and characteristics of TACE in teleosts is unclear. In this study, we report the cloning of a cDNA sequence of the PaTACE from ayu, Plecoglossus altivelis. PaTACE encodes an 865-aa...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2014.10.010

    authors： Lu XJ,Chen Q,Yang GJ,Chen J

    更新日期：2015-02-01 00:00:00

  • Differential expression profiles and gene structure of two tumor necrosis factor-alpha variants in Atlantic salmon (Salmo salar L.).

    abstract：:Here we present the gene structure and expression data on two Atlantic salmon TNF-alpha genes. Both genes are approximately 2.0kb in length and organized into four exons and three introns. The open reading frame of both genes translates into 246 amino acid putative peptides, being 91.5% identical at the amino acid lev...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2006.08.015

    authors： Haugland O,Mercy IS,Romøren K,Torgersen J,Evensen O

    更新日期：2007-03-01 00:00:00

  • Increased PD-1+CD154+ Tfh cells are possibly the most important functional subset of PD-1+ T follicular helper cells in adult patients with minimal change disease.

    abstract：:T follicular helper (Tfh) cells, especially programmed cell death protein 1 (PD-1)+ Tfh cells, exert important functions in the normal immune response. The purpose of this study was to determine the frequency of different subsets of PD-1+ Tfh cells and their functional effects in adult patients with minimal change dis...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2017.12.020

    authors： Li T,Shi Y,Sun W,Wang H,Wang Q,Jiang Y

    更新日期：2018-02-01 00:00:00

  • TWEAK/Fn14 promotes pro-inflammatory cytokine secretion in hepatic stellate cells via NF-κB/STAT3 pathways.

    abstract：:Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) and its receptor fibroblast growth factor-inducible 14 (Fn14) have been associated with liver disease. Hepatic stellate cells (HSCs) play a critical role in the hepatic wound-healing response after liver injury, but there is little information available on t...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2017.04.003

    authors： Wang A,Zhang F,Xu H,Xu M,Cao Y,Wang C,Xu Y,Su M,Zhang M,Zhuge Y

    更新日期：2017-07-01 00:00:00

  • Using agonists for iNKT cells in cancer therapy.

    abstract：:The capacity of α-galactosylceramide (α-GalCer) to act as an anti-cancer agent in mice through the specific stimulation of type I NKT (iNKT) cells has prompted extensive investigation to translate this finding to the clinic. However, low frequencies of iNKT cells in cancer patients and their hypo-responsiveness to rep...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2020.12.010

    authors： Painter GF,Burn OK,Hermans IF

    更新日期：2021-02-01 00:00:00

  • Autologous anti-metatype immune response in rabbits.

    abstract：:Rabbits hyperimmunized with fluorescyl-conjugated KLH exhibited bound ligand associated with a high affinity circulating IgG anti-fluorescein population. After cessation of immunogen administration the liganded complexes were eventually spontaneously cleared from the circulation. Individual rabbits synthesized autolog...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(92)90105-7

    authors： Voss EW Jr,Moore JK,Weidner-McGufficke KM,Denzin LK,Bedzyk WD,Voss VH

    更新日期：1992-02-01 00:00:00

  • HMGB1 induced inflammatory effect is blocked by CRISPLD2 via MiR155 in hepatic fibrogenesis.

    abstract：:A number of studies have showed that High mobility group box-1 (HMGB1), which played key role in inflammation activation by triggering the toll like receptor 4 (TLR4) signaling axis in hepatic fibrogenesis, may share similar inflammation stimulating mechanism with LPS. Herein, we introduced a recently established anti...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2015.10.018

    authors： Zhang H,Liu Z,Liu S

    更新日期：2016-01-01 00:00:00

  • A new effector of lipid metabolism: complement factor properdin.

    abstract：BACKGROUND:The complement system is well known for its role in innate immunity via the classical, the alternative and the lectin pathways, although recent investigations suggest expanding roles in adipose tissue. Properdin stabilizes C3 convertase following alternative complement activation. Properdin is also present i...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2012.02.110

    authors： Gauvreau D,Roy C,Tom FQ,Lu H,Miegueu P,Richard D,Song WC,Stover C,Cianflone K

    更新日期：2012-05-01 00:00:00

  • MYC inhibition increases PD-L1 expression induced by IFN-γ in hepatocellular carcinoma cells.

    abstract：:The effectiveness of immunotherapy targeting the immune checkpoint PD-L1/PD-1 pathway highlights importance of elucidating the regulatory mechanisms of PD-L1 expression in cancer cells. Previous studies demonstrate that oncogene MYC up-regulates PD-L1 expression in lymphomas. In the present study, we investigated the ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2018.07.006

    authors： Zou J,Zhuang M,Yu X,Li N,Mao R,Wang Z,Wang J,Wang X,Zhou H,Zhang L,Shi Y

    更新日期：2018-09-01 00:00:00

  • Antigen degradation or presentation by MHC class I molecules via classical and non-classical pathways.

    abstract：:Major histocompatibility complex (MHC) class I molecules usually present endogenous peptides at the cell surface. This is the result of a cascade of events involving various dedicated proteins like the peptide transporter associated with antigen processing (TAP) and the ER chaperone tapasin. However, alternative ways ...

    journal_title：Molecular immunology

    pub_type： 杂志文章,评审

    doi：10.1016/s0161-5890(02)00101-3

    authors： Grommé M,Neefjes J

    更新日期：2002-10-01 00:00:00

  • Complement regulates inhalation tolerance at the dendritic cell/T cell interface.

    abstract：:Pulmonary exposure to innocuous aeroallergens is a common event leading to inhalation tolerance. Distinct subsets of pulmonary dendritic cells (DC) and regulatory T cells (T(Reg)) play critical roles in mediating and maintaining such tolerance. In asthmatics, the same aeroallergens drive a maladaptive, Th2-biased immu...

    journal_title：Molecular immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molimm.2006.06.016

    authors： Köhl J,Wills-Karp M

    更新日期：2007-01-01 00:00:00

  • Structural variants of Salmonella Typhimurium lipopolysaccharide induce less dimerization of TLR4/MD-2 and reduced pro-inflammatory cytokine production in human monocytes.

    abstract：:Salmonella enterica serovar Typhimurium (S. Typhimurium) changes the structure of its lipopolysaccharide (LPS) in response to the environment. The two main LPS variants found in S. Typhimurium correspond to LPS with a hepta-acylated lipid A (LPS 430) and LPS with modified phosphate groups on its lipid A (LPS 435). We ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2019.03.003

    authors： Aldapa-Vega G,Moreno-Eutimio MA,Berlanga-Taylor AJ,Jiménez-Uribe AP,Nieto-Velazquez G,López-Ortega O,Mancilla-Herrera I,Cortés-Malagón EM,Gunn JS,Isibasi A,Wong-Baeza I,López-Macías C,Pastelin-Palacios R

    更新日期：2019-07-01 00:00:00

  • MOTS-c peptide increases survival and decreases bacterial load in mice infected with MRSA.

    abstract：:Sepsis is a life-threatening disease characterized by uncontrolled inflammatory responses upon pathogen infections, especially for the antibiotic-resistant strains, such as Methicillin-resistant S. aureus (MRSA). Here we demonstrated that a Mitochondria-derived peptide (MOTS-c) could significantly improve the survival...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2017.10.017

    authors： Zhai D,Ye Z,Jiang Y,Xu C,Ruan B,Yang Y,Lei X,Xiang A,Lu H,Zhu Z,Yan Z,Wei D,Li Q,Wang L,Lu Z

    更新日期：2017-12-01 00:00:00

  • Clinical relevance and prognostic significance of PD-1/PD-Ls in non-metastatic bladder cancer: A role for PD-L2.

    abstract：BACKGROUND:Bladder cancer (BC) can be successfully treated by manipulating immune responses with intravesical Bacillus Calmette-Guerin instillation or targeting the PD-1/PD-L signaling pathway. In the present study we investigated the prognostic significance of the immune checkpoint inhibitor PD-1 and its ligands PD-L1...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2020.05.010

    authors： Ariafar A,Ghaedi M,Rezaeifard S,Shahriari S,Zeighami S,Ghaderi A,Faghih Z

    更新日期：2020-08-01 00:00:00

  • PECAM-1-regulated signalling thresholds control tolerance in anergic transgenic B-cells.

    abstract：:Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1/CD31) is an immunoglobulin (Ig)-immunoreceptor tyrosine based inhibitory motif (Ig-ITIM) superfamily member that recruits and activates protein-tyrosine phosphatases, predominantly SHP-2 and to a lesser extent, SHP-1. Previously, we have shown that deletion of PEC...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2007.09.024

    authors： Wong MX,Hayball JD,Jackson DE

    更新日期：2008-03-01 00:00:00

  • Identification of six novel CC chemokines in gilthead seabream (Sparus aurata) implicated in the antiviral immune response.

    abstract：:Chemokines are key regulators of migration and consequent activation of migrating leukocytes. CC chemokines constitute the largest chemokine group with 24-28 members in mammalian species, and even more in teleost fish, with up to 81 members in zebrafish Danio rerio. Further studies concerning fish chemokine genes will...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2009.12.014

    authors： Cuesta A,Dios S,Figueras A,Novoa B,Esteban MA,Meseguer J,Tafalla C

    更新日期：2010-03-01 00:00:00