Abstract：
:Human beta defensin-1 (hBD1) is a component of the immune system which links the innate and adaptive immune responses. We have demonstrated that hBD1 induces rapid cytolysis of prostate cancer cells and that it may also possess tumor suppressive abilities. In addition, there is a high frequency of cancer-specific loss of hBD1 expression which further suggests its potential role in tumor progression. However, the factors responsible for the loss of hBD1 expression are not known. PAX2, a transcriptional regulator normally expressed during early development, has been implicated as an oncogene in carcinomas of the kidney, prostate, breast and ovary. It is known that expression of PAX2 in these tumor cells mediates the evasion of cell death through the suppression of cell death pathways involving the p53 tumor suppressor. However, we have demonstrated that knock-down of PAX2 expression results in cell death independent of p53 status, thus suggesting that additional cell death pathways are negatively regulated by PAX2. Here we describe a novel pathway in which PAX2 represses hBD1 expression through binding of the PAX2 homeodomain to the hBD1 promoter. Furthermore, knock-down of PAX2 expression results in the re-expression of hBD1, and subsequently prostate cancer cell death. These findings are the first to demonstrate that the PAX2 oncogene suppresses hBD1 expression in cancer and further implicate PAX2 as a novel therapeutic target for prostate cancer treatment.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Bose SK,Gibson W,Bullard RS,Donald CDdoi
10.1016/j.molimm.2008.11.004subject
Has Abstractpub_date
2009-03-01 00:00:00pages
1140-8issue
6eissn
0161-5890issn
1872-9142pii
S0161-5890(08)00750-5journal_volume
46pub_type
杂志文章abstract：:CD55 is a key regulator of complement activation, expressed on most tissues and cells in man and other mammals. In the rat, alternative splicing in the gene encoding CD55 yields GPI-anchored (GPI-CD55) and transmembrane (TM-CD55) forms. Published Northern blot analysis indicated that while GPI-CD55 was broadly express...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.018
更新日期：2007-03-01 00:00:00
abstract：:CD8+ cytolytic T lymphocytes are essential players of anti-tumor immune responses. On tumors, they recognize peptides of about 8-to-10 amino acids that generally result from the degradation of cellular proteins by the proteasome. Until a decade ago, these peptides were thought to solely correspond to linear fragments ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2018.03.030
更新日期：2019-09-01 00:00:00
abstract：:T regulatory (Treg)/T-helper (Th) 17 imbalance has been shown to integrate with epigenetics to result in the development of autoimmune diseases. We aim to investigate the influence of disease staging on Treg/Th17 cells and whether the aberrant DNA methylation is implicated in the development of rheumatoid arthritis (R...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.08.002
更新日期：2020-11-01 00:00:00
abstract：:The carbohydrate binding specificity of the basic lectin from winged bean (Psophocarpus tetragonolobus) was investigated by quantitative precipitin analysis using blood group A, B, H, Le and I substances and by precipitation inhibition with various mono- and oligosaccharides. The lectin precipitated best with A1 subst...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90101-6
更新日期：1989-02-01 00:00:00
abstract：:To date, although a promising anti-inflammatory activity of oligomeric proanthocyanidins (OPCs) has been observed in asthma, the mechanism responsible for these immunomodulatory properties remains obscure. Dendritic cells (DCs) that reside in the airway have been widely perceived as an important contributor to asthma....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.09.012
更新日期：2017-11-01 00:00:00
abstract：:Earlier studies, which provided indirect evidence for the involvement of the C gamma 2 domain of human immunoglobulin G (IgG) in human immunoglobulin G (IgG) in human monocyte binding, have been extended to further localise the site of interaction on human IgG. A number of IgGs from several different species and fragm...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90059-3
更新日期：1986-03-01 00:00:00
abstract：:Effective immunization with short polypeptide antigens has typically only been possible when the peptide is conjugated to a large carrier substance, usually a protein. Such immunizations suffer from difficulties in producing conjugates of reliable composition, and from unwanted anti-carrier immune responses. When a ch...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90084-1
更新日期：1984-01-01 00:00:00
abstract：:Subunits of the mitochondrial ATP synthase complex are expressed on the surface of tumors, bind the TCR of human Vgamma9/Vdelta2 lymphocytes and promote their cytotoxicity. Present experiments show that detection of the complex (called ecto-F1-ATPase) at the cell surface by immunofluorescence correlates with low MHC-c...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.05.026
更新日期：2008-01-01 00:00:00
abstract：:Multiple organ dysfunction syndrome (MODS) is a major cause of morbidity and mortality in surgical intensive care units. It is characterized by progressive failure of two or more organs remote from the origin of injury. Since MODS results from a severe generalized inflammatory response, both chemokines and complement ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(96)00077-6
更新日期：1996-10-01 00:00:00
abstract：:T-lymphocyte proliferative responses were determined with peripheral blood lymphocytes (PBL), peritoneal exudate lymphocytes (PEL) and lymph node lymphocytes (LNL) of guinea pigs immunized with horse cytochrome c (HCytc). The proliferative response of PBL activated by peptide 81-104 from animals immunized with HCytc w...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90115-0
更新日期：1988-08-01 00:00:00
abstract：:C-reactive protein (CRP) binds to chromatin, histones, and small nuclear ribonucleoproteins (snRNPs) through a phosphocholine (PC)-inhibitable, calcium-dependent binding site. snRNPs process pre-mRNA to mature mRNA and are composed of small uridine-rich RNAs (designated U1, U2, U5 and U4/U6) and associated proteins. W...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90141-w
更新日期：1993-06-01 00:00:00
abstract：:Aging is an irreversible physiological process characterized by increased risk of diseases, reduced effectiveness of vaccines, and decreased immune responses. One of the most prominent paradigms of aging and age related conditions is the progressive accumulation of un-repaired DNA breaks leading to apoptosis and exhau...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.07.001
更新日期：2013-01-01 00:00:00
abstract：:Concanavalin A (Con A)section-binding proteins from mouse spleen leukocytes were characterised by two-dimensional electrophoresis of material precipitated, by Con A plus anti-Con A, from lysates of biosynthetically-labelled cells. Although most cell surface (iodinatable) proteins are known to bind Con A, some of the m...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90030-5
更新日期：1983-04-01 00:00:00
abstract：:Tumor necrosis factor-alpha (TNF) has been implicated in the pathogenesis of a variety of human diseases including septic shock, cachexia, graft-versus-host disease and several autoimmune diseases. Monoclonal antibodies directed against TNF provide an attractive mode of therapeutic intervention in these diseases. We h...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90106-l
更新日期：1993-11-01 00:00:00
abstract：:Monoclonal antibodies to cyclosporine A (Cs), a potent immunosuppressant, were generated in BALB/c mice using a novel antigen prepared by linking Cs to a protein carrier via a photoactive cross-linking reagent, 4-benzoylbenzoic acid (BBa). Twenty-two monoclonal anti-Cs antibodies were generated, using Cs-BBa-bovine se...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90162-q
更新日期：1992-01-01 00:00:00
abstract：:Cutaneous leishmaniasis (CL) mainly caused by Leishmania braziliensis is a chronic inflammatory disease widely spread in Brazil. Genetic variant strains of this parasite have been associated with atypical clinical manifestations of CL in an endemic area in Brazil. Furthermore, these strains have presented distinct bio...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.12.013
更新日期：2019-02-01 00:00:00
abstract：:The thermal effects associated with the binding reaction of concanavalin A and immunoglobulins have been measured by calorimetry. IgG solutions do not generate heat on mixing with concanavalin A, confirming the low reactivity of IgG for the lectin molecule. IgM solutions, on the other hand, show a substantial enthalpi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90357-1
更新日期：1982-07-01 00:00:00
abstract：:The antigenic structural features of alpha-lactalbumin have been investigated using a radioimmunoassay, peptide inhibition of the quantitative precipitin reaction, and by immunodiffusion analysis after chemical modification of the molecule. Antigenic activity (in rabbits) was localized to several peptic fragments and ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90192-4
更新日期：1982-11-01 00:00:00
abstract：:Several mutations in the CFH gene have been described in non-Shiga-toxin-associated haemolytic uraemic syndrome (non-Stx-HUS), a rare syndrome characterized by haemolytic anaemia, thrombocytopenia and acute renal failure. Mutations in genes encoding other complement regulatory proteins, membrane cofactor protein (CD46...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.004
更新日期：2007-03-01 00:00:00
abstract：:In this paper, we performed further biochemical characterization of the CD26 antigen, as defined by the mAbs in anti-1F7 and anti-Ta1, in order to clarify the observed functional differences among these mAbs. For this purpose, we developed a mAb, anti-5F8, which recognizes yet another epitope on the CD26 antigen diffe...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90099-j
更新日期：1992-02-01 00:00:00
abstract：BACKGROUND:Individuals genetically deficient of properdin are more susceptible to meningococcal disease. Likewise low concentration or decreased biological activity of mannose-binding lectin (MBL) is associated with higher incidence of bacterial infections during childhood. In this study we report our findings in a Dan...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.02.017
更新日期：2006-02-01 00:00:00
abstract：OBJECTIVE:Nuclear components targeted by autoantibodies are a characteristic feature of the autoimmune disease systemic lupus erythematosus (SLE). The nucleosome, a major autoantigen, is released in patients with SLE as a result of a disturbed apoptosis and/or an insufficient clearance of apoptotic debris. During apopt...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.08.003
更新日期：2010-11-01 00:00:00
abstract：:A number of proteins with significant similarity to the tumour necrosis factor (TNF) have been identified over the last years. Upon interaction with their cognate receptor (members of the TNF-receptor family), all members of this protein family induce either cell death or proliferation/differentiation of the receptor-...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00016-8
更新日期：1995-07-01 00:00:00
abstract：:We describe the entire immunoglobulin heavy chain (IgH) locus from the reptile Anolis carolinensis. The heavy chain constant (C(H)) region includes C mu, C delta and C upsilon genes. This is the first description of a C upsilon gene in the reptilian class. Variable (V(H)), diversity (D(H)) and joining (J(H)) genes are...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.02.019
更新日期：2009-05-01 00:00:00
abstract：:The isolation and characterization of two human serum proteins, RHP and N-RHP, are described. N-RHP appears to be the normal counterpart of RHP which is found at elevated levels in sera of patients with rheumatoid arthritis [Rosano et al. (1988b) Inflammation 12, 351 - 360]. Although both proteins crossreact with anti...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00068-2
更新日期：1995-11-01 00:00:00
abstract：:A channel catfish genomic lambda library was screened with CH and JH probes which were derived from our earlier sequence analyses on different full-length heavy chain cDNA clones. One clone, designated C7, contained a genomic insert of about 18 kb and hybridized with specific probes for each of the four domains of the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90096-g
更新日期：1992-02-01 00:00:00
abstract：:Based on our findings that HIV-1 gp41 independently of CD4, can bind to the helper T-cell line H9, we characterized putative binding of HIV-1 gp41 to B-cell lines, Raji, Bjab and Ramos. Using fluorescence-activated cell sorter (FACS) we examined the binding of soluble gp41 (sgp41; Env amino acid 539-684) to these B-ce...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90134-w
更新日期：1993-09-01 00:00:00
abstract：:TRAF6 is a key player at the cross-roads of development and immunity. The analysis of its in vivo molecular function is a great challenge since severe developmental defects and early lethality caused by Traf6 deficiency in knock-out mice interfere with analyses of the immune response. In this study we have used a new ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.08.011
更新日期：2010-11-01 00:00:00
abstract：:Complement per se has been shown to play an important role in demyelinating disease but controversy remains regarding the role of C3 in the development and progression of experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis. In this study, we used C3(-/-) mice to confirm previous fi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.02.002
更新日期：2007-05-01 00:00:00
abstract：:The complement system plays a central role in a number of human inflammatory diseases, and there is a significant need for development of complement-directed therapies. The discovery of an arsenal of anti-complement proteins secreted by the pathogen Staphylococcus aureus brought with it the potential for harnessing th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.05.012
更新日期：2015-10-01 00:00:00
