Abstract:
:Based on our findings that HIV-1 gp41 independently of CD4, can bind to the helper T-cell line H9, we characterized putative binding of HIV-1 gp41 to B-cell lines, Raji, Bjab and Ramos. Using fluorescence-activated cell sorter (FACS) we examined the binding of soluble gp41 (sgp41; Env amino acid 539-684) to these B-cell lines. Using sgp41 attached to sepharose beads Raji cell lysates were absorbed. The sgp41-eluate of Raji cell lysates could inhibit the sgp41-binding to Raji cells. By SDS-PAGE of sgp41-eluate of Raji cell lysates four strong protein band, 37, 45, 49 and 62 kD, and a weak band of 92 kD were stained with Coomassie blue. By Western blot (ligand blot) analysis using sgp41 four protein bands, 37, 45, 49 and 62 kD, were observed in sgp41-eluate of Raji cell lysates. To test the individual proteins the five proteins were isolated from the sgp41-eluate of Raji cell lysates. Three proteins, 45, 49 and 62 kD, each could partially inhibit the sgp41-binding to Raji cells. The results suggest that these three proteins in Raji cell lysates are possible candidates for the putative gp41 receptor(s).
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Chen YH,Böck G,Vornhagen R,Steindl F,Katinger H,Dierich MPdoi
10.1016/0161-5890(93)90134-wsubject
Has Abstractpub_date
1993-09-01 00:00:00pages
1159-63issue
13eissn
0161-5890issn
1872-9142journal_volume
30pub_type
杂志文章abstract::Stimulation of cells of the rat basophilic leukemia line RBL-2H3, which are used as a model in biochemical studies of mast cells, by antigen or by the calcium ionophore ionomycin, are known to cause secretion of mediators of inflammation. These stimuli have now been found to cause a decrease in the cells' cytosolic pH...
journal_title:Molecular immunology
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journal_title:Molecular immunology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2017-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90156-6
更新日期:1993-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.molimm.2007.08.011
更新日期:2008-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90194-3
更新日期:1992-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:1992-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90047-f
更新日期:1993-10-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,随机对照试验
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更新日期:2019-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 历史文章,杂志文章,评审
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更新日期:2015-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.09.001
更新日期:2013-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.09.016
更新日期:2018-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.01.023
更新日期:2017-06-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.06.003
更新日期:2007-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1983-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90133-5
更新日期:1983-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.06.021
更新日期:2017-08-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90082-m
更新日期:1993-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2012-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2016-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.01.031
更新日期:2008-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(82)90357-1
更新日期:1982-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 信件
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更新日期:2008-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.01.009
更新日期:2007-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2019.06.018
更新日期:2019-08-01 00:00:00
abstract::A major goal in HIV-1 vaccine research is to develop an immunogen that can elicit broadly neutralizing antibodies that efficiently neutralize a wide range of the HIV-1 subtypes. Using biopanning procedure we have selected linear peptide VGAFGSFYRLSVLQS mimicking the structure of discontinuous binding sites of broadly ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.01.003
更新日期:2012-04-01 00:00:00