Calorimetric study of the binding reaction of concanavalin A with immunoglublins.

abstract：:Light chains of 426 randomly selected myelomas from BALB/c and NZB mice were characterized by isoelectric focusing (IF) and electrophoresis at pH 3. Approximately 50% of the light chains in each strain were found to have unique electrophoretic properties. The remaining 50% of light chains fell into groups of two or mo...

journal_title：Molecular immunology

authors： Gibson DM

更新日期：1984-05-01 00:00:00

abstract：:The functional importance of various cell membrane bound molecules was studied and compared in the NK cytotoxicity and CTL activity. LFA-1 and CD2 participate in both killing functions, while CD3 and CD8/CD4 as well as MHC class I molecules are involved only in CTL activity. Nevertheless CD2- and beta 2-microglobulin ...

journal_title：Molecular immunology

authors： Petrányi GG,Pócsik E,Kotlán B,Görög G,Benczur M

更新日期：1986-11-01 00:00:00

abstract：:Anti-human C1s monoclonal antibody H1532, a mouse gamma-1-immunoglobulin elicited by a C1r2C1s2 immunogen, appeared to bind to the beta-domain of C1s by electron microscopy. In agreement with this observation, Western blotting demonstrated good binding to unreduced C1s, but no binding to the alpha or gamma-B domains. ...

journal_title：Molecular immunology

authors： Tseng Y,Phillips ML,Schumaker VN

更新日期：1997-06-01 00:00:00

abstract：:Mutations within the 5'-non-coding region of the bcl-6 gene can occur in lymphomas that originate from germinal centers (GCs), as well as in normal memory and GC B cells. Mutations in the p53 gene occur in 50% of human cancers. Since both bcl-6 and p53 can be mutated in certain circumstances, we investigated the accum...

journal_title：Molecular immunology

authors： Yavuz AS,Monson NL,Yavuz S,Grammer AC,Longo N,Girschick HJ,Lipsky PE

更新日期：2002-11-01 00:00:00

abstract：:The outer membrane protein A (OmpA) is one of the intra-species conserved proteins with immunogenicity widely found in the family of Enterobacteriaceae. Here we first confirmed OmpA is conserved in the three pathogenic Yersinia: Yersinia pestis, Yersinia pseudotuberculosis and pathogenic Yersinia enterocolitica, with ...

journal_title：Molecular immunology

authors： Chen Y,Duan R,Li X,Li K,Liang J,Liu C,Qiu H,Xiao Y,Jing H,Wang X

更新日期：2015-12-01 00:00:00

abstract：:GK-1 is an immunomodulatory, 18-aa-long peptide that has been proved to promote the activation of mouse peritoneal macrophages and LPS-pulsed mouse bone marrow-derived dendritic cells (BM-DCs). This study is aimed to explore the mechanisms underlying the activation of these antigen-presenting cells (APCs) by GK-1. In ...

journal_title：Molecular immunology

authors： Montero L,Cervantes-Torres J,Sciutto E,Fragoso G

更新日期：2020-12-01 00:00:00

abstract：:We have previously generated human IgG1 antibodies that were engineered for reduced binding to the classical Fcγ receptors (FcγRI-III) and C1q, thereby eliminating their destructive effector functions (constant region G1Δnab). In their potential use as blocking agents, favorable binding to the neonatal Fc receptor (Fc...

journal_title：Molecular immunology

authors： Stapleton NM,Armstrong-Fisher SS,Andersen JT,van der Schoot CE,Porter C,Page KR,Falconer D,de Haas M,Williamson LM,Clark MR,Vidarsson G,Armour KL

更新日期：2018-03-01 00:00:00

abstract：:The disialoganglioside GalAcbeta1-4(NeuAcalpha2-8NeuAcalpha2-3)Galbeta1-4Glcbeta1-1Cer (GD2) is expressed on various tumors, including neuroblastoma, and was defined as a relevant tumor antigen. The monoclonal anti-GD2 antibody 14.18 is widely used for diagnostic purposes in neuroblastoma, and in its mouse/human chime...

journal_title：Molecular immunology

authors： Förster-Waldl E,Riemer AB,Dehof AK,Neumann D,Brämswig K,Boltz-Nitulescu G,Pehamberger H,Zielinski CC,Scheiner O,Pollak A,Lode H,Jensen-Jarolim E

更新日期：2005-02-01 00:00:00

abstract：:Structural similarities between two members of the immunoglobulin superfamily were explored by making chimeric immunoglobulin/CD4 antigen molecules. A crossover in the middle of the originally proposed J kappa homology unit of the first domain of the CD4 molecule was used to construct a chimeric molecule having human ...

journal_title：Molecular immunology

authors： Frey T,Estess P,Oi VT

更新日期：1993-06-01 00:00:00

abstract：:Degenerate PCR was used to isolate a 221-base pair nucleotide sequence of a new crustin-like antibacterial peptide from the haemocytes of the European lobster, Homarus gammarus. Rapid amplification of cDNA ends was used to extend the sequence to determine the complete open reading frame and un-translated regions. The ...

journal_title：Molecular immunology

authors： Hauton C,Brockton V,Smith VJ

更新日期：2006-03-01 00:00:00

abstract：:Dendritic cells (DC) survey epithelial or mucosal surfaces for antigens, take them up via their endocytic or phagocytic receptors, process the captured antigens and migrate to the lymphatic tissues. In the draining lymph nodes they present the immunogenic peptides to T cells thereby inducing antigen-specific immune re...

journal_title：Molecular immunology

authors： Wilflingseder D,Banki Z,Dierich MP,Stoiber H

更新日期：2005-02-01 00:00:00

abstract：:Ig production by splenic human B cells that express different surface Ig isotypes were analysed in limiting dilution cultures. Therefore, FACS sorted IgM+, IgG+ and IgA1+ B cells were stimulated with PMA-activated EL4 thymoma cells as helper cells in the presence of IL-2 and IL-4. We found that at least every second B...

journal_title：Molecular immunology

authors： Brinkmann V,Müller S,Heusser CH

更新日期：1992-10-01 00:00:00

abstract：:The A/J murine hybridoma cell line 40-150 secretes antidigoxin antibodies with high affinity for digoxin. A first-order spontaneous mutant (40-150 A2.4) produces antibodies containing a mutation at heavy chain position 94 resulting in reduced affinity for digoxin. A second-order mutant (40-150 A2.4 P.10) derived from ...

journal_title：Molecular immunology

authors： Shaw SY,Margolies MN

更新日期：1992-04-01 00:00:00

abstract：:Human CD63 has been reported to play a role either as an inhibitor or as a co-stimulator of T- cell responses, although the mechanism of this is unclear. In this study, an anti-human CD63 monoclonal antibody (mAb) COS3A was used to monitor the role of CD63 in T-cell activation. MAb COS3A could inhibit CD3-mediated T-c...

journal_title：Molecular immunology

authors： Wansook S,Mahasongkram K,Chruekamlow N,Pata S,Kasinrerk W,Khunkaewla P

更新日期：2019-10-01 00:00:00

abstract：:Human leukocyte antigens (HLA) allele determination is becoming an increasingly important aspect in the field of transplantation as well as in the area of HLA association with a number of diseases. Through Lebanon's history, this country, situated at a crossroads between Europe, Asia and Africa, has been a host for va...

journal_title：Molecular immunology

authors： Samaha H,Rahal EA,Abou-Jaoude M,Younes M,Dacchache J,Hakime N

更新日期：2003-07-01 00:00:00

abstract：:MHC class I molecules present short peptides, usually 8-10 amino acids in length, to CD8(+) T cells. These peptides are typically generated from full-length endogenously synthesized proteins degraded by the antigen processing machinery of the target cell. However, exogenous proteins, whether originating from intracell...

journal_title：Molecular immunology

authors： Wolf BJ,Princiotta MF

更新日期：2011-01-01 00:00:00

abstract：:Lactobacilli are probiotic bacteria that are considered to be beneficial in the gastrointestinal tract of humans. Although lactobacilli are well known to alleviate intestinal inflammation, the molecular basis of this phenomenon is poorly understood. In this study, we investigated the effect of Lactobacillus plantarum ...

journal_title：Molecular immunology

authors： Noh SY,Kang SS,Yun CH,Han SH

更新日期：2015-03-01 00:00:00

abstract：:Deficiencies in the classical pathway of complement activation have some common features but show also great differences. Deficiencies of each of the components (C1q, C1s, C1r, C4 and C2) imply increased susceptibility to bacterial infections. They are also associated with increased risk to develop systemic lupus eryt...

journal_title：Molecular immunology

authors： Truedsson L

更新日期：2015-11-01 00:00:00

abstract：:When mouse dendritic cells (DCs) are isolated from tissues, purified and placed in a nutritive culture they die more rapidly than would be expected from their normal turnover in vivo. This can distort culture assays of DC function. We therefore tested several approaches to prolonging DC survival in culture. Of several...

journal_title：Molecular immunology

authors： Vremec D,Hansen J,Strasser A,Acha-Orbea H,Zhan Y,O'Keeffe M,Shortman K

更新日期：2015-02-01 00:00:00

abstract：:HLA-B27 (B27) is strongly associated with the spondyloarthritides. B27 is expressed at the cell surface of antigen presenting cells (APC) both as canonical β2m-associated and non-canonical β2m-free heavy chain (FHC) forms which include B27 dimers (termed B272). B27 FHC forms arise in an endosomal compartment from recy...

journal_title：Molecular immunology

authors： Shaw J,Hatano H,Kollnberger S

更新日期：2014-01-01 00:00:00

abstract：:Purified mRNA's encoding the HLA-A and -B antigen heavy chains or beta 2-microglobulin were prepared from human B lymphoid cells by positive hybridization selection procedures. The role of chain association in the biosynthesis and intracellular transport of HLA-A and -B antigens was investigated by injecting these mRN...

journal_title：Molecular immunology

authors： Kinnon C,Owen MJ

更新日期：1986-05-01 00:00:00

abstract：:Leprosy is a bacterial disease caused by M. leprae. Its clinical spectrum reflects the host's immune response to the M. leprae and provide an ideal model to investigate the host pathogen interaction and immunological dysregulation. Tregs are high in leprosy patients and responsible for immune suppression of the host b...

journal_title：Molecular immunology

authors： Tarique M,Saini C,Naqvi RA,Khanna N,Sharma A,Rao DN

更新日期：2017-03-01 00:00:00

abstract：:Dehydroepiandrosterone (DHEA) has anti-inflammatory, anti-oxidant and immune-regulating properties, while the mechanism of DHEA actions remains unclear. The present study aims to investigate the effect and possible mechanism of DHEA on immune function of mice in vivo and in vitro. In vivo, a lipopolysaccharide (LPS)-i...

journal_title：Molecular immunology

authors： Cao J,Yu L,Zhao J,Ma H

更新日期：2019-08-01 00:00:00

abstract：:Attempts to develop peptide vaccines, based on a limited number of peptides face two problems: HLA polymorphism and the high mutation rate of viral epitopes. We have developed a new genomic method that ensures maximal coverage and thus maximal applicability of the peptide vaccine. The same method also promises a large...

journal_title：Molecular immunology

authors： Vider-Shalit T,Raffaeli S,Louzoun Y

更新日期：2007-02-01 00:00:00

abstract：:UL16 binding proteins (ULBPs) are ligands for the NK cell activating receptor NKG2D. A cDNA encoding a porcine ULBP-like protein (PULBP) was cloned and the predicted amino acid sequence exhibited 35-52% identity to human ULBPs. Southern blot analysis suggested that there is only one ULBP-like gene in the pig genome. T...

journal_title：Molecular immunology

authors： García-Borges CN,Phanavanh B,Saraswati S,Dennis RA,Crew MD

更新日期：2005-04-01 00:00:00

abstract：:Inhibition of C5a by antibodies has been demonstrated to dramatically improve survival in various sepsis models in mice and rats. The structural basis of C5a mediated bioactivity and C5a antibody mediated neutralization are of interesting to be investigated. In the previous study, we obtained a novel functional mouse ...

journal_title：Molecular immunology

authors： Wei H,Lin Z,Feng J,Peng H,Guo R,Han G,Geng S,Lang X,Sun Y,Shen B,Li Y

更新日期：2011-07-01 00:00:00

abstract：:Bcl-2 translocations in follicular lymphomas (FL) are often associated with downstream immunoglobulin class switch recombination (CSR) on the translocated allele. We studied cell lines with different translocations into the IgH locus to gauge any common features associated with downstream CSR events. CSR associated wi...

journal_title：Molecular immunology

authors： Hughes JA,Weckert HA,van Holst Pellekaan C,Benson EM,Dunn IS

更新日期：2003-12-01 00:00:00

abstract：:The developmental regulation of antigen receptor gene transcription and recombination are mediated by cis regulatory elements. At the T cell receptor beta chain locus (TCRbeta), two DNase I hypersensitive sites within the Jbeta2-Cbeta2 intron contained binding sites for NF-kappaB and additional nuclear factors and wer...

journal_title：Molecular immunology

authors： Whitehurst CE,Hu H,Ryu CJ,Rajendran P,Schmidt T,Chen J

更新日期：2001-01-01 00:00:00

abstract：:The recovery of VDJ rearrangement is most often accomplished by PCR amplification of DNA extracted from mixtures of B-cells. Using this procedure in swine, VDJs containing chimeric V(H) genes that resemble gene-conversion products, are frequently encountered. To examine whether these chimeras could be the result of PC...

journal_title：Molecular immunology

authors： Sinkora M,Sun J,Butler JE

更新日期：2000-12-01 00:00:00

abstract：:Major histocompatibility complex (MHC) class I and II proteins have been in the focus of interest for immunologists, biochemists, cell biologists, and structural biologists for decades. With dozens of entries in the Protein Data Bank, their crystal structures are now sufficiently well understood, while their dynamic p...

journal_title：Molecular immunology

authors： Yaneva R,Schneeweiss C,Zacharias M,Springer S

更新日期：2010-01-01 00:00:00