Probing the structure of C1 with an anti-C1s monoclonal antibody: the possible existence of two forms of C1 in solution.


:Anti-human C1s monoclonal antibody H1532, a mouse gamma-1-immunoglobulin elicited by a C1r2C1s2 immunogen, appeared to bind to the beta-domain of C1s by electron microscopy. In agreement with this observation, Western blotting demonstrated good binding to unreduced C1s, but no binding to the alpha or gamma-B domains. When added to solutions of the C1r2C1s2 tetramer, HI532 converted the 8.7 S tetramer into an 18 S complex, which was seen by electron microscopy to be a dimer of parallel C1s x C1r x C1r x C1s molecules cross-linked by two bivalent monoclonal antibodies. If increasing amounts of HI532 were added to C1r2C1s2 followed by addition of equivalent C1q, there was a progressive loss of hemolytic activity, which became zero when two equivalents of antibody HI532 were added. When two equivalents of HI532 were added to serum or C1 reconstituted overnight from purified subcomponents, there was an immediate loss of approximately 50% of the hemolytic activity; thereafter, activity decayed slowly and even after 24 hr, 10-30% of the activity remained. The rapid loss of only 50% of the activity would be readily explained by the existence of two conformations of C1, one of which was rapidly disassembled by antibody, and the other was resistant to disassembly. These two conformations may correspond to two previously proposed structures for the C1 complex.


Mol Immunol


Molecular immunology


Tseng Y,Phillips ML,Schumaker VN




Has Abstract


1997-06-01 00:00:00












  • Familial C3 glomerulonephritis caused by a novel CFHR5-CFHR2 fusion gene.

    abstract::C3 glomerulopathy (C3G) is an ultra-rare complement-mediated renal disease characterized histologically by the predominance of C3 deposition within in the glomerulus. Familial cases of C3G are extremely uncommon and offer unique insight into the genetic drivers of complement dysregulation. In this report, we describe ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Xiao X,Ghossein C,Tortajada A,Zhang Y,Meyer N,Jones M,Borsa NG,Nester CM,Thomas CP,de Córdoba SR,Smith RJ

    更新日期:2016-09-01 00:00:00

  • C5a- and ASP-mediated C5L2 activation, endocytosis and recycling are lost in S323I-C5L2 mutation.

    abstract:UNLABELLED:C5L2, a G-protein-coupled receptor (GPCR), has been identified as an ASP (C3adesArg) and C5a receptor. Controversy exists regarding both ligand binding and functionality. ASP activation of C5L2 is proposed to regulate fat storage. C5L2 is also proposed as a decoy receptor for C5a, an inflammatory mediator, b...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Cui W,Simaan M,Laporte S,Lodge R,Cianflone K

    更新日期:2009-09-01 00:00:00

  • Increase of the stimulatory effect of dendritic cells by pulsing with apoptotic bodies transfected with the MHC class II gene.

    abstract::Dendritic cells (DC) are capable of capturing and processing antigens and can stimulate co-cultured effector cells in a specific manner. Here, we pulsed DC with apoptotic bodies (apb) from colorectal carcinoma. For enhancement of the immunogenic potential of apb, we transduced the tumor cells with the MHC class II gen...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Märten A,Renoth S,Schmidt-Wolf IG

    更新日期:2002-11-01 00:00:00

  • Identification of novel non-pathogenic mutation in SH3 domain of Btk in an XLA patient.

    abstract::A first report of an XLA patient with a polymorphism in Btk SH3 domain has been identified after sequencing of the entire gene. SH3 domain variants might not be detected due to well characterized mutations outside the domain. ...

    journal_title:Molecular immunology

    pub_type: 信件


    authors: Pérez de Diego R,Bravo J,Allende LM,López-Granados E,Rivera J,Ferreira A,Fontán G,García Rodríguez MC

    更新日期:2008-01-01 00:00:00

  • Hydrophobic strip-of-helix algorithm for selection of T cell-presented peptides.

    abstract::In extension of the hypothesis that an amphipathic alpha helix of Ii (Phe146-Val164) bound to the foreign antigen-presenting site (desetope) of class II MHC molecules through hydrophobic amino acid residues (Phe146, Leu150, Leu153, Met157, Ile160, Val164) which were present in an axial strip along one side of the Ii h...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Stille CJ,Thomas LJ,Reyes VE,Humphreys RE

    更新日期:1987-10-01 00:00:00

  • BST-2/tetherin: structural biology, viral antagonism, and immunobiology of a potent host antiviral factor.

    abstract::BST-2 (also known as tetherin, CD317, or HM1.24) was first described as a potent interferon-inducible host antiviral factor nearly five years ago. Since that time, numerous reports have been published regarding the antiviral activity and immunological properties of this protein. BST-2 blocks viral replication by inhib...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Swiecki M,Omattage NS,Brett TJ

    更新日期:2013-06-01 00:00:00

  • The dual specificity phosphatase transcriptome of the murine thymus.

    abstract::Properly regulated mitogen-activated protein (MAP) kinase activity is critical for normal thymocyte development. MAP kinases are activated by phosphorylation of tyrosine and threonine, and dual specificity phosphatases (DUSPs) can inactivate MAP kinases by dephosphorylating both tyrosine and threonine. However, a role...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Tanzola MB,Kersh GJ

    更新日期:2006-02-01 00:00:00

  • Maleimide conjugation markedly enhances the immunogenicity of both human and murine idiotype-KLH vaccines.

    abstract::The collection of epitopes present within the variable regions of the tumor-specific clonal immunoglobulin expressed by B cell lymphomas (idiotype, Id) can serve as a target for active immunotherapy. Traditionally, tumor-derived Id protein is chemically conjugated to the immunogenic foreign carrier protein keyhole lim...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Kafi K,Betting DJ,Yamada RE,Bacica M,Steward KK,Timmerman JM

    更新日期:2009-01-01 00:00:00

  • NCCRP-1: a novel receptor protein sequenced from teleost nonspecific cytotoxic cells.

    abstract::The recognition structure responsible for binding to conventional antigen on target cells has not previously been described for nonspecific cytotoxic cells (NCC) or for mammalian natural killer (NK) cells. Although several biochemical pathways may be available for initiation of the lytic cycle in NCC, evidence present...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Jaso-Friedmann L,Leary JH 3rd,Evans DL

    更新日期:1997-08-01 00:00:00

  • Comparative properties of monoclonal antibodies comprising a high-affinity anti-fluorescyl idiotype family.

    abstract::The T-dependent BALB/c murine immune response to fluorescein (F1) is characterized by structural heterogeneity at the protein level exemplified in part by a significantly wide range of affinities (Ka), and apparent lack of dominant idiotypes. In order to generate an idiotype family, xenogenic anti-idiotype (anti-ID) a...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Bates RM,Ballard DW,Voss EW Jr

    更新日期:1985-08-01 00:00:00

  • Endothelial P2Y2 receptor regulates LPS-induced neutrophil transendothelial migration in vitro.

    abstract::Previous studies showed that P2 receptors are involved in neutrophil migration via stimulation of chemokine release and by facilitating chemoattractant gradient sensing. Here, we have investigated whether these receptors are involved in LPS-induced neutrophil transendothelial migration (TEM) using a Boyden chamber whe...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Kukulski F,Ben Yebdri F,Bahrami F,Fausther M,Tremblay A,Sévigny J

    更新日期:2010-02-01 00:00:00

  • Variable epitope library-based vaccines: shooting moving targets.

    abstract::While the antigenic variability is the major obstacle for developing vaccines against antigenically variable pathogens (AVPs) and cancer, this issue is not addressed adequately in current vaccine efforts. We developed a novel variable epitope library (VEL)-based vaccine strategy using immunogens carrying a mixture of ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Pedroza-Roldan C,Charles-Niño C,Saavedra R,Govezensky T,Vaca L,Avaniss-Aghajani E,Gevorkian G,Manoutcharian K

    更新日期:2009-12-01 00:00:00

  • BRAF splice variants in rheumatoid arthritis synovial fibroblasts activate MAPK through CRAF.

    abstract::Rheumatoid arthritis (RA) is a destructive polyarthritis in which synovial-like fibroblasts (SFs) invade and erode cartilage by expressing membrane-anchored type 1 matrix metalloproteinase (MT1-MMP). The mitogen activated protein kinase (MAPK) pathway is activated in RA SFs, but the mechanism of activation is unknown....

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Weisbart RH,Chan G,Li E,Farmani N,Heinze E,Rubell A,Nishimura RN,Colburn K

    更新日期:2013-10-01 00:00:00

  • Single amino acid substitutions in V(H) CDR2 are sufficient to generate or enhance the specificity of two forms of an anti-arsonate antibody variable region for DNA.

    abstract::We previously showed that a variety of amino acid substitutions at positions 58 and 59 in the V(H) CDR2 of an anti-arsonate (Ars) antibody Fab simultaneously resulted in increased or unaltered affinity for Ars and substantially enhanced affinity for DNA. To test the generality of these observations, we generated and c...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Hande S,Manser T

    更新日期:1997-12-01 00:00:00

  • Quasi-elastic light scattering of antigen-antibody complexes.

    abstract::Many biological properties of immune complexes (IC) depend upon their size. Quasi-elastic light scattering (QLS) was used to measure a mean equivalent hydrodynamic radius (Rh) and variance of the distribution of model IC composed of bovine serum albumin (BSA) as antigen (Ag) and combinations of two or three well-chara...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Yarmush DM,Murphy RM,Colton CK,Fisch M,Yarmush ML

    更新日期:1988-01-01 00:00:00

  • Allogeneic and syngeneic class I MHC complexes drive the association of CD8 and TCR on 2C T cells.

    abstract::In most cases, cytotoxic T cell activation is dependent on the interaction of the T cell receptor (TCR) and CD8 with MHC class I molecules. In the CD8(+) T cell system based on the mouse cytotoxic T cell clone 2C, recognition of the allogeneic MHC L(d) exhibits a less significant role for CD8 than recognition of the s...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Lee PU,Kranz DM

    更新日期:2003-01-01 00:00:00

  • A model system for T-lymphocyte differentiation: regulation of CD4 and CD8 gene expression in SL12.4 T-lymphoma cell clones.

    abstract::The T-cell surface proteins CD4 (L3T4) and CD8 (Lyt2) are first expressed on thymocytes as they undergo maturation in the thymus. Two immature T-lymphoma cell clones SL12.4 and RS4.2 which constitutively express low or undetectable levels of CD4 and CD8 were used to investigate the activation of CD4 and CD8 gene expre...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Wilkinson MF,Fong AM,Huynh H,Hays EF,MacLeod CL

    更新日期:1991-01-01 00:00:00

  • Effect of C1-INH on ischemia/reperfusion injury in a porcine limb ex vivo perfusion model.

    abstract::Revascularization of an amputated limb within 4-6h is essential to avoid extensive ischemia/reperfusion (I/R) injury leading to vascular leakage, edema and tissue necrosis. I/R injury is a pathological inflammatory condition that occurs during reperfusion of an organ or tissue after prolonged ischemia. It is character...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Abdelhafez MM,Shaw J,Sutter D,Schnider J,Banz Y,Jenni H,Voegelin E,Constantinescu MA,Rieben R

    更新日期:2017-08-01 00:00:00

  • Cloning of a cDNA for a T cell produced molecule with a putative immune regulatory role.

    abstract::An expression cDNA library was constructed from the helper T cell hybridoma, A.1.1, which has been shown to produce constitutively proteins involved in the down regulation of the immune response. From this library we identified and characterized a cDNA clone, J6B7, by screening with a polyclonal antibody specific for ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Lee C,Ghoshal K,Beaman KD

    更新日期:1990-11-01 00:00:00

  • Immunological relationships among group I and group V allergens from grass pollen.

    abstract::Specific IgE antibodies have been affinity-purified from recombinant grass pollen allergens, and used to identify isoforms of the two major allergens of rye-grass pollen, Lol p I and Lol p V and cross-reactive allergens in other grasses. Lol p I-specific IgE (affinity-purified from the recombinant protein expressed by...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Smith PM,Ong EK,Knox RB,Singh MB

    更新日期:1994-04-01 00:00:00

  • A simple procedure for the isolation of human secretory IgA of IgA1 and IgA2 subclass by a jackfruit lectin, jacalin, affinity chromatography.

    abstract::Jackfruit lectin, jacalin, prepared from two batches of jackfruit seeds showed a different specificity in precipitating reaction in Agarose gel with various purified immunoglobulins and secretory components. Jacalin-P, extracted from jackfruit seeds from the Philippines, reacts only with serum IgA and secretory IgA of...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Kondoh H,Kobayashi K,Hagiwara K

    更新日期:1987-11-01 00:00:00

  • Generation of a comprehensive panel of crustacean allergens from the North Sea Shrimp Crangon crangon.

    abstract:BACKGROUND:Published data on crustacean allergens are incomplete. The identification of tropomyosin (TM), arginine kinase (AK), sarcoplasmic Ca-binding protein (SCP) and myosin light chain (MLC) as shrimp allergens are all important contributions but additional allergens are required for the development of a complete s...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Bauermeister K,Wangorsch A,Garoffo LP,Reuter A,Conti A,Taylor SL,Lidholm J,Dewitt AM,Enrique E,Vieths S,Holzhauser T,Ballmer-Weber B,Reese G

    更新日期:2011-09-01 00:00:00

  • Antigenic peptide binding to MHC class II molecules at increased peptide concentrations.

    abstract::Affinity-purified major histocompatibility complex (MHC) class II molecules are known to bind antigenic peptides in vitro. The percentage of MHC class II molecules occupied with such peptides is usually very low and varies significantly depending upon the sequence and size of a given antigenic peptide. The present stu...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Nag B,Mukku PV,Arimilli S,Phan D,Deshpande SV,Winkelhake JL

    更新日期:1994-10-01 00:00:00

  • Toward an atomistic understanding of the immune synapse: large-scale molecular dynamics simulation of a membrane-embedded TCR-pMHC-CD4 complex.

    abstract::T-cell activation requires interaction of T-cell receptors (TCR) with peptide epitopes bound by major histocompatibility complex (MHC) proteins. This interaction occurs at a special cell-cell junction known as the immune or immunological synapse. Fluorescence microscopy has shown that the interplay among one agonist p...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Wan S,Flower DR,Coveney PV

    更新日期:2008-03-01 00:00:00

  • Biological and molecular docking studies on coagulin-H: Human IL-2 novel natural inhibitor.

    abstract::Withanolide, coagulin-H (1), was evaluated for its effect on various cellular functions related to immune response including lymphocyte proliferation, and expression of interleukin-2 (IL-2) cytokine, and results were compared with prednisolone (2), a commonly used immune modulating drug. Coagulin-H (1) was found to ha...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Mesaik MA,Zaheer-Ul-Haq,Murad S,Ismail Z,Abdullah NR,Gill HK,Atta-Ur-Rahman,Yousaf M,Siddiqui RA,Ahmad A,Choudhary MI

    更新日期:2006-04-01 00:00:00

  • Analysis and characterization of the expression of the secretory and membrane forms of IgM heavy chains in the pufferfish, Takifugu rubripes.

    abstract::We investigated the structure and expression of immunoglobulin genes in the pufferfish, Takifugu rubripes, a highly prized and economically important fish species. The cDNA fragment that partially encodes the constant region of the IgM heavy chain was isolated in these animals by RACE using degenerate primers after wh...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Saha NR,Suetake H,Suzuki Y

    更新日期:2005-01-01 00:00:00

  • Human monoclonal antibodies produced in transgenic BABkappa,lambda mice recognising idiotypic immunoglobulins of human lymphoma cells.

    abstract::Clonal idiotypic immunoglobulins of follicular lymphomas can be isolated by somatic fusion procedures. Idiotypic IgMs (Id-IgM) were isolated from two patients and used to immunise a strain of mice, deficient in mouse antibody production and engineered with yeast artificial chromosomes (YAC) containing fragments of the...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Suárez E,Yáñez R,Barrios Y,Díaz-Espada F

    更新日期:2004-07-01 00:00:00

  • MicroRNA-146a promotes IgE class switch in B cells via upregulating 14-3-3σ expression.

    abstract::B cells play a critical role in immune responses both in physiological and pathological conditions, and microRNAs have been shown to play important roles in regulating B cell proliferation and function. MiR-146a has been shown to modulate T cell immunity, but its function in regulating B cell response remains partiall...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Li F,Huang Y,Huang YY,Kuang YS,Wei YJ,Xiang L,Zhang XJ,Jia ZC,Jiang S,Li JY,Wan Y

    更新日期:2017-12-01 00:00:00

  • Contribution of secondary Igkappa rearrangement to primary immunoglobulin repertoire diversification.

    abstract::Abs reactive to DNA and DNA/histone complexes are a distinguished characteristic of primary immunoglobulin repertoires in autoimmune B6.MRL-Faslpr and MRL/MpJ-Faslpr mice. These mice are defective in Fas receptor, which is critical for the apoptosis of autoreactive B cells by an extrinsic pathway. In the present study...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Li S,Liu W,Li Y,Zhao S,Liu C,Hu M,Yue W,Liu Y,Wang Y,Yang R,Xiang R,Liu F

    更新日期:2016-10-01 00:00:00

  • Effects of antibody affinity and antigen valence on molecular forms of immune complexes.

    abstract::The effect of antibody affinity on molecular forms of immune complexes was investigated by measuring antigen-antibody interactions using surface plasmon resonance (SPR), electrospray ionization time-of-flight mass spectrometry under non-denaturing conditions (MS), analytical ultracentrifugation (AUC), and transmission...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Oda M,Uchiyama S,Noda M,Nishi Y,Koga M,Mayanagi K,Robinson CV,Fukui K,Kobayashi Y,Morikawa K,Azuma T

    更新日期:2009-12-01 00:00:00