Abstract:
:Activation-induced cell death (AICD) in T lymphocytes depends on the expression of Fas-ligand, which triggers the apoptotic process after binding to its receptor Fas. This leads to the activation of cysteine proteases of the caspase family and especially of caspase-3, a critical effector protein during AICD. We have previously observed the up-regulation of caspase-3 expression in effector but not memory T cells stimulated in vivo. In this study, we further characterized the regulation of caspase expression following T cell receptor (TCR) signaling and demonstrate that a three-fold increase in caspase-3 mRNA levels was observed by semi-quantitative and real-time RT-PCR analysis. Caspase-3 expression was selectively increased among five different caspases following TCR stimulation, as assessed by RNase protection assay. Real-time RT-PCR analysis demonstrated that a three-fold up-regulation in caspase-3 mRNA levels was observed following TCR triggering, whereas caspase-8 mRNA levels remained unchanged. The increase in caspase-3 mRNA levels occurred before cleavage and activation of caspase-3 and in the absence of apoptosis. TCR-mediated induction in caspase-3 expression was not dependent on STAT1 activation, since following stimulation of KOX-14 cells the transcription factor was not phosphorylated. Together, these results show that TCR activation triggers the selective increase in caspase-3 mRNA levels, independently of caspase activity and the induction of apoptosis.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Sabbagh L,Bourbonnière M,Sékaly RP,Cohen LYdoi
10.1016/j.molimm.2004.12.011subject
Has Abstractpub_date
2005-07-01 00:00:00pages
1345-54issue
11eissn
0161-5890issn
1872-9142pii
S0161-5890(05)00009-Xjournal_volume
42pub_type
杂志文章abstract::Cytohesin-1 is a guanine nucleotide exchange factor for ADP ribosylation factor 6 (Arf6) in human blood neutrophils and differentiated PLB-985 neutrophil-like cells. Cytohesin-1 regulates adhesion and the transendothelial migration of monocytes, dendritic cells and T lymphocytes through activation of the β2 integrin L...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.03.018
更新日期:2011-07-01 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
abstract::Placental growth factor (PlGF), a member of vascular endothelial growth factors (VEGF) family, is considered as an important antigen associated with pathological conditions such as cancer cell growth, and metastasis. PlGF-targeting via nanobody (Nb) therefore could be beneficial to modulate these pathologies. In this ...
journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2016-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(82)90263-2
更新日期:1982-12-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2018.10.019
更新日期:2018-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90086-7
更新日期:1988-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(89)90035-7
更新日期:1989-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(94)90106-6
更新日期:1994-10-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.09.024
更新日期:2009-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(97)00042-4
更新日期:1997-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(99)00034-6
更新日期:1999-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.09.033
更新日期:2007-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(85)90043-4
更新日期:1985-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.06.437
更新日期:2011-09-01 00:00:00
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pub_type: 历史文章,杂志文章,评审
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.08.001
更新日期:2006-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90130-2
更新日期:1989-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.08.015
更新日期:2007-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2004.09.029
更新日期:2005-05-01 00:00:00
abstract::The recovery of VDJ rearrangement is most often accomplished by PCR amplification of DNA extracted from mixtures of B-cells. Using this procedure in swine, VDJs containing chimeric V(H) genes that resemble gene-conversion products, are frequently encountered. To examine whether these chimeras could be the result of PC...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
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更新日期:2000-12-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.09.003
更新日期:2011-10-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(86)90139-2
更新日期:1986-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.08.010
更新日期:2007-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(01)00042-6
更新日期:2001-08-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(90)90110-l
更新日期:1990-02-01 00:00:00