Abstract：
:Cytohesin-1 is a guanine nucleotide exchange factor for ADP ribosylation factor 6 (Arf6) in human blood neutrophils and differentiated PLB-985 neutrophil-like cells. Cytohesin-1 regulates adhesion and the transendothelial migration of monocytes, dendritic cells and T lymphocytes through activation of the β2 integrin LFA-1. In this study we investigated the role of cytohesin-1 in neutrophil and neutrophil-like cell adhesion to HUVECs, immobilized ICAM-1, and the α4β1 and α5β1 integrin extracellular matrix ligand fibronectin. We show that cytohesin-1 knockdown or inhibition with secinH3 inhibits fMLF-mediated cell adhesion to HUVECs and immobilized ICAM-1, whereas cytohesin-1 over-expression has the opposing effect. Binding of PLB-985 cells to HUVECs correlated with expression of the high-affinity β2 integrin epitope recognized by mAb24. Adhesion to HUVECs was inhibited by soluble ICAM-1, anti-ICAM-1, anti-CD11a and anti-CD18, but not anti-CD11b, blocking antibodies. We also demonstrate that cytohesin-1 knockdown promotes fMLF-mediated cell adhesion to fibronectin whereas cytohesin-1 over-expression has the opposing effect. Crosstalk between β1 and β2 integrins also exists since inhibition of β1 integrin functions with blocking antibodies enhanced adhesion of PLB-985 over-expressing cytohesin-1 to ICAM-1. We suggest that cytohesin-1 is a key regulator of neutrophil adhesion to endothelial cells and to components of extracellular matrix, which may influence cell emigration through its dual opposing effect on β2 and β1 integrin activation.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
El azreq MA,Bourgoin SGdoi
10.1016/j.molimm.2011.03.018subject
Has Abstractpub_date
2011-07-01 00:00:00pages
1408-16issue
12-13eissn
0161-5890issn
1872-9142pii
S0161-5890(11)00112-Xjournal_volume
48pub_type
杂志文章abstract：:Linear IgE-binding epitopes identified in legumin allergens of peanut (Ara h 3) and other allergenic tree nuts (Jug r 4 of walnut, Cor a 9 of hazelnut, Ana o 2 cashew nut) were mapped on three-dimensional models of the proteins built up by homology modelling. A conformational analysis revealed that consensual surface-...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.01.023
更新日期：2007-05-01 00:00:00
abstract：:A study aiming at cloning and characterizing natural antibodies to human immunodeficiency virus type 1 (HIV-1) targets is described. In particular, we report the molecular cloning of a Fab molecule binding the HIV-1/Tat protein from a seronegative patient. The Fab was characterized for its binding specificity and inve...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.08.003
更新日期：2006-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00079-0
更新日期：1998-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(94)90115-5
更新日期：1994-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.molimm.2015.06.015
更新日期：2015-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(86)90099-4
更新日期：1986-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 临床试验,杂志文章
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更新日期：2015-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(83)90029-9
更新日期：1983-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.molimm.2013.06.016
更新日期：2013-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.10.020
更新日期：2006-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.05.026
更新日期：2008-01-01 00:00:00
abstract：:The porcine complement proteins C3 and C4 have been isolated and then characterized using small-angle scattering methods. Within the limits of experimental errors, the porcine proteins are virtually identical with the corresponding human proteins as measured in terms of mol. wt, Mr and radius of gyration, R,: Mr(C3) =...
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pub_type： 杂志文章
doi：10.1016/0161-5890(91)90181-i
更新日期：1991-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90130-2
更新日期：1989-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.07.018
更新日期：2016-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.06.012
更新日期：2008-09-01 00:00:00
abstract：:Mannan-binding lectin (MBL) is a plasma protein of the innate immune system with the ability to initiate antimicrobial and inflammatory actions. MBL deficiency is common. More than 10% of the general population may, depending on definition, be classified as MBL deficient, underlining the redundancy of the immune syste...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2005.06.018
更新日期：2006-01-01 00:00:00
abstract：:Most cells are believed to be capable of producing type I interferons (IFN I) as part of an innate immune response against, for instance, viral infections. In macrophages, IFN I is potently induced upon cytoplasmic exposure to foreign nucleic acids. Infection of these cells with herpesviruses leads to triggering of th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.08.025
更新日期：2017-11-01 00:00:00
abstract：:A monoclonal antibody (vpg15) has been described which can block infection with feline immunodeficiency virus (FIV) and which recognizes the feline homologue of CD9. In order to study the role of feline CD9 in infection with FIV we have molecularly cloned a cDNA encoding feline CD9 by R.A.C.E (rapid amplification of c...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00008-3
更新日期：1995-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.06.013
更新日期：2020-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.molimm.2010.11.011
更新日期：2011-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(03)00226-8
更新日期：2003-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(90)90170-5
更新日期：1990-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.10.018
更新日期：2006-04-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(02)00101-3
更新日期：2002-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.09.028
更新日期：2008-03-01 00:00:00
abstract：:High glucose- induced endothelial dysregulation has been recognized as an initiation of vascular complications in Type 2 diabetes mellitus (T2DM). Anagliptin is a novel licensed dipeptidyl peptidase-4 (DPP-4) inhibitor for the treatment of T2DM. The effects of anagliptin in high glucose- induced endothelial dysfunctio...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.01.006
更新日期：2019-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.04.002
更新日期：2012-07-01 00:00:00
abstract：:We report that the protein tyrosine phosphatase PTP-PEST is expressed in resting human and mouse CD4(+) and CD8(+) T cells, but not in Jurkat T leukemia cells, and that PTP-PEST protein, but not mRNA, was dramatically downregulated in CD4(+) and CD8(+) primary human T cells upon T cell activation. This was also true i...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.03.019
更新日期：2008-06-01 00:00:00
abstract：:Degenerate PCR was used to isolate a 221-base pair nucleotide sequence of a new crustin-like antibacterial peptide from the haemocytes of the European lobster, Homarus gammarus. Rapid amplification of cDNA ends was used to extend the sequence to determine the complete open reading frame and un-translated regions. The ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.07.029
更新日期：2006-03-01 00:00:00
abstract：:Selection of specific antibodies from large repertoires is of importance in generating antibodies to specific structural determinants and in studying structure-function relationships. Alkaline phosphatase (AP) has several isozymes with various degrees of homology and a range of common synthetic substrates. We have pre...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.02.024
更新日期：2007-01-01 00:00:00
