Abstract：
:Withanolide, coagulin-H (1), was evaluated for its effect on various cellular functions related to immune response including lymphocyte proliferation, and expression of interleukin-2 (IL-2) cytokine, and results were compared with prednisolone (2), a commonly used immune modulating drug. Coagulin-H (1) was found to have a powerful inhibitory effect on lymphocyte proliferation and Th-1 cytokine production. Inhibition of the phytohaemagglutinin (PHA)-activated T-cell proliferation by coagulin-H (1) was observed in a concentration dependent manner. A complete suppression of PHA-activated T-cell was observed at > or =2.5 microg/mL concentrations of compound (1) and this suppression activity was similar to that of prednisolone (2). Coagulin-H (1) also significantly inhibited IL-2 production by 80%. The interactions of coagulin-H (1) (a natural inhibitor) and prednisolone (2) (a drug) to IL-2 were also investigated in order to understand the differences in their effects on T-cell responses. This paper also describes the results of molecular docking study on IL-2 inhibition. Docking studies predicted that coagulin-H (1) binds to receptor binding site of IL-2 more effectively than prednisolone (2). Based on the computational and the experimental results, coagulin-H (1) was identified as a potential immunosuppressive candidate.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Mesaik MA,Zaheer-Ul-Haq,Murad S,Ismail Z,Abdullah NR,Gill HK,Atta-Ur-Rahman,Yousaf M,Siddiqui RA,Ahmad A,Choudhary MIdoi
10.1016/j.molimm.2005.10.020subject
Has Abstractpub_date
2006-04-01 00:00:00pages
1855-63issue
11eissn
0161-5890issn
1872-9142pii
S0161-5890(05)00372-Xjournal_volume
43pub_type
杂志文章abstract：:A channel catfish genomic lambda library was screened with CH and JH probes which were derived from our earlier sequence analyses on different full-length heavy chain cDNA clones. One clone, designated C7, contained a genomic insert of about 18 kb and hybridized with specific probes for each of the four domains of the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90096-g
更新日期：1992-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2006-02-01 00:00:00
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更新日期：2017-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：1986-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：1988-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.molimm.2020.11.014
更新日期：2021-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90131-3
更新日期：1987-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：1982-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：1993-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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更新日期：2005-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90001-0
更新日期：1987-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.09.014
更新日期：2020-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 信件
doi：10.1016/j.molimm.2007.07.005
更新日期：2008-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2015.03.246
更新日期：2015-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90015-p
更新日期：1992-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(97)00109-0
更新日期：1997-08-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.06.008
更新日期：2014-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2007-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.08.001
更新日期：2006-03-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2002-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.08.011
更新日期：2008-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.06.012
更新日期：2014-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.12.020
更新日期：2018-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.03.007
更新日期：2008-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.10.001
更新日期：2011-01-01 00:00:00
abstract：:The generation of T cell receptor (TCR) sequence diversity can produce 'forbidden' clones able to recognize self-antigens. Here, the structure of the complex between a myelin basic protein peptide (MBP85-99), human leukocyte antigen (HLA)-DR2 (DRB1*1501/DRA) and TCR-Ob.2F3, the dominant autoimmune clone obtained from ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.07.010
更新日期：2010-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.03.006
更新日期：2020-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2010.06.003
更新日期：2010-09-01 00:00:00
abstract：:In human peripheral blood, most of the CD3+ cells express the alpha/beta T cell receptor. A smaller fraction of CD3+ T cells express the gamma/delta T cell receptor (from 1 to 10% depending the individuals, with an average of 3-5%). Interestingly, although the alpha/beta + T cells never express the gamma chain at the ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/0161-5890(92)90001-e
更新日期：1992-04-01 00:00:00
