Abstract：
:The generation of T cell receptor (TCR) sequence diversity can produce 'forbidden' clones able to recognize self-antigens. Here, the structure of the complex between a myelin basic protein peptide (MBP85-99), human leukocyte antigen (HLA)-DR2 (DRB1*1501/DRA) and TCR-Ob.2F3, the dominant autoimmune clone obtained from a multiple sclerosis (MS) patient, has been determined using structural docking simulation and dynamics in silico and compared to the structure of TCR-Ob.1A12 complexes with the same MHC/peptide determined by X-ray crystallography. The two TCRs differ by three amino acids in the CDR3 α and β loops. As the result different hydrogen bonds are formed between the two CDR3β loops and the peptide in the complexes of the simulated structures, with three hydrogen bonds seen in the TCR-Ob.2F3 complex and five in the TCR-Ob.1A12 complex. The two TCRs, each located near the N-terminal end of the HLA-DR2 binding groove and both had an orthogonal binding axis but they deviated by about 10°. Simulation methods, such as structural docking and molecular dynamics as used here, provide an avenue to understand molecular binding mode efficiently and more rapidly than obtaining multiple crystal structures when a large structural database is already available.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Kato Z,Stern JN,Nakamura HK,Miyashita N,Kuwata K,Kondo N,Strominger JLdoi
10.1016/j.molimm.2010.07.010subject
Has Abstractpub_date
2010-11-01 00:00:00pages
314-20issue
1-3eissn
0161-5890issn
1872-9142pii
S0161-5890(10)00524-9journal_volume
48pub_type
杂志文章abstract：:Dendritic cells(DCs) were once considered as a single cell type closely related developmentally to macrophages. Now we recognise several subtypes of DCs and have outlined several different pathways that potentially lead to their development. This article outlines some of the research findings that led to these changes...
journal_title：Molecular immunology
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.molimm.2019.12.016
更新日期：2020-03-01 00:00:00
abstract：:The collection of epitopes present within the variable regions of the tumor-specific clonal immunoglobulin expressed by B cell lymphomas (idiotype, Id) can serve as a target for active immunotherapy. Traditionally, tumor-derived Id protein is chemically conjugated to the immunogenic foreign carrier protein keyhole lim...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.10.020
更新日期：2009-01-01 00:00:00
abstract：:Cimetidine, a histamine type-2 receptor antagonist, is known to inhibit the growth of several tumors in human and animals, however the mechanism of action underlying this effect remains largely unknown. Here, in the mice model of 3LL lung tumor, cimetidine showed significant inhibition of tumor growth. However, an in ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.10.035
更新日期：2013-05-01 00:00:00
abstract：:SOCS3 is well known to negatively regulate various cytokine-mediated signaling responses, but its direct role in the expression of specific cytokines has not been clearly elucidated. To understand the role of SOCS3 in the expression of IL-4, one of the key Th2 cytokines, RBL-2H3 cells (a rat mast cell line) were engin...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.11.005
更新日期：2011-02-01 00:00:00
abstract：:Arginine kinase (AK) is a panallergen present in crustaceans, which can induce an immunoglobulin (Ig) E-mediated immune response in humans. The aim of this work was to map and characterize the antigenic epitopes of Scylla paramamosain AK. Specific-protein-A-enriched IgG raised in rabbits against purified S. paramamosa...
journal_title：Molecular immunology
pub_type： 临床试验,杂志文章
doi：10.1016/j.molimm.2015.02.010
更新日期：2015-06-01 00:00:00
abstract：:In addition to the antigen receptor, resting T cells express a number of receptors that can be stimulated to generate proliferative signals. These "accessory" receptors require co-expression of the T cell receptor (TCR), suggesting that they channel their signals via secondary activation of the signal transduction fun...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)00160-3
更新日期：1995-04-01 00:00:00
abstract：:Subclasses of IgG were separated from pools of mouse sera by letting immunoglobulins absorb on protein A-Sepharose and by eluting with buffers of decreasing pH. Most donor mice were immunized with a conjugate of a hapten (NIP) and chicken gamma globulin 20 days previously. The results indicate that concentrations of I...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90062-7
更新日期：1983-03-01 00:00:00
abstract：:The interaction between a single-chain Fv (sFv) of the monoclonal antibody 3A21 and its antigen, bovine pancreatic ribonuclease A (RNase A), was studied by site-directed mutagenesis of the hypervariable regions and fluorescence polarization analysis. The affinity constants of wild-type sFv and a mutant sFv D31A (Asp31...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(97)00094-1
更新日期：1997-07-01 00:00:00
abstract：:The effect of antibody affinity on molecular forms of immune complexes was investigated by measuring antigen-antibody interactions using surface plasmon resonance (SPR), electrospray ionization time-of-flight mass spectrometry under non-denaturing conditions (MS), analytical ultracentrifugation (AUC), and transmission...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.09.009
更新日期：2009-12-01 00:00:00
abstract：:Idiotype (Id) and autoanti-thyroglobulin were induced in different strains of mice by priming with anti-Id to monoclonal anti-thyroglobulins (D8 and G4) and challenged with a subimmunogenic dose of thyroglobulin (Tg). Both D8.Id and G4.Id were induced in CBA mice by priming with the appropriate anti-Id, but only primi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90159-2
更新日期：1985-03-01 00:00:00
abstract：:In the previous study, antibodies against CD3 molecule have been produced and were used in labeling T cells in Japanese flounder (Paralichthys olivaceus). In this paper, CD4+ and CD8+ lymphocytes subpopulations in peripheral blood leucocytes (PBL), spleen and head kidney of flounder were investigated. The flounder CD4...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.02.015
更新日期：2017-05-01 00:00:00
abstract：:Surfactant protein D (SP-D) interacts with various different microorganisms and plays an important role in pulmonary innate immunity. SP-D expression has also been detected in extrapulmonary tissues, including the gastro-intestinal tract. However, its function in the intestine is unknown and may differ considerably fr...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.03.013
更新日期：2007-07-01 00:00:00
abstract：:Peripheral blood B lymphocytes have been isolated from healthy individuals who were immunized with lymphocytes from HLA-incompatible donors and transformed with Epstein-Barr virus to produce human monoclonal cell lines specific for human HLA molecules. The cell lines have been previously characterized and are known to...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(95)00071-2
更新日期：1995-10-01 00:00:00
abstract：:Light chains of 426 randomly selected myelomas from BALB/c and NZB mice were characterized by isoelectric focusing (IF) and electrophoresis at pH 3. Approximately 50% of the light chains in each strain were found to have unique electrophoretic properties. The remaining 50% of light chains fell into groups of two or mo...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90040-3
更新日期：1984-05-01 00:00:00
abstract：:To investigate the IL-6/IL-6 receptor system, we obtained five murine monoclonal antibodies against human IL-6, which neutralize its biological activity. We classified them into two groups (Type I mAb and Type II mAb) according to the epitopes they recognized. These two types of antibodies showed no difference in the ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90001-z
更新日期：1991-11-01 00:00:00
abstract：:Four monoclonal antibodies (MAbs) directed against the P1 blood group antigen were produced by hybridomas obtained from mouse immunized with turtle-dove avomucoid. One of the MAb (154 IX B6) selected as a blood typing reagent agglutinated native P1 and Pk1 red cells with a high titer but was inactive against native P2...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90089-7
更新日期：1987-02-01 00:00:00
abstract：:The scid mutation interferes with normal rearrangement of antigen receptor genes, leading to an absence of T and B lymphocytes in most SCID mice. However, the SCID phenotype is "leaky", with an age- and strain-dependent increase in the incidence of mice with small number of T and B cells and readily detectable serum i...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90016-7
更新日期：1994-08-01 00:00:00
abstract：:An important function of Fcγ receptors is the removal of IgG-containing immune complexes from the circulation. The activating receptor FcγRIIA and inhibitory receptor FcγRIIB2 are both expressed on human myeloid cells, and are both capable of mediating endocytosis of immune complexes. We studied endocytosis of these t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.09.003
更新日期：2011-10-01 00:00:00
abstract：:In this paper we report a chemometric approach to Quantitative Structure-Activity Relationship (QSAR) analysis applied to a study of the binding of peptides to Major Histocompatibility Complex (MHC) class I proteins. Peptides which possess the known primary anchor residue motif for HLA-B27 binding do not necessarily b...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90042-6
更新日期：1994-05-01 00:00:00
abstract：:Infectious salmon anaemia virus (ISAV) is a marine orthomyxovirus of significant interest not only as a cause of a fatal disease of farmed Atlantic salmon resulting in severe economic losses to the aquaculture industry, but also as the only poikilothermic orthomyxovirus. ISAV targets vascular endothelial cells and mac...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.06.015
更新日期：2009-09-01 00:00:00
abstract：:Leprosy is a bacterial disease caused by M. leprae. Its clinical spectrum reflects the host's immune response to the M. leprae and provide an ideal model to investigate the host pathogen interaction and immunological dysregulation. Tregs are high in leprosy patients and responsible for immune suppression of the host b...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.01.008
更新日期：2017-03-01 00:00:00
abstract：:Effective use of adenovirus-5 (Ad5) in cancer therapy is heavily dependent on the degree to which the virus's natural tropism can be subverted to one that favours tumour cells. This is normally achieved through either engineering of the viral fiber knob or the use of bispecific adaptors that display both adenovirus an...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.08.014
更新日期：2015-12-01 00:00:00
abstract：:Whether bovine myelin basic protein (BP) and bovine spinal cord protein (SCP) cross-react at the humoral immune level was assessed with a sensitive solid-phase enzyme immunoassay. We found that a hyperimmune anti-SCP serum reacted strongly with SCP and cross-reacted nearly as well with BP. A hyperimmune anti-BP serum ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90159-7
更新日期：1984-10-01 00:00:00
abstract：:In an attempt to understand the relationship of amino acid sequence to the formation of primary or multiple myeloma-related amyloid (AL amyloid), we have determined the complete amino acid sequence of amyloid protein BAN. This protein belongs to the kappa I immunoglobulin light chain subgroup and has a polypeptide cha...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90173-2
更新日期：1986-01-01 00:00:00
abstract：:A 30,000 mol. wt component (beta) is associated in a 1:1 ratio with the 50,000 mol. wt glycoprotein (alpha) which binds immunoglobulin E (IgE) on mast cells and related tumor cells. We show that alpha and beta are associated in membrane preparations. This is consistent with previous results which showed labeling of be...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90334-0
更新日期：1982-02-01 00:00:00
abstract：:Dense deposit disease (DDD) is strongly associated with the uncontrolled activation of the complement alternative pathway. Factor H (CFH)-deficient (Cfh(-/-)) mice spontaneously develop C3 deposition along the glomerular basement membrane (GBM) with subsequent development of glomerulonephritis with features of DDD, a ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.03.030
更新日期：2009-06-01 00:00:00
abstract：:The structural characteristics of antigens recognized by a panel of monoclonal antibodies prepared against a rabbit T-lymphocyte cell line have been investigated. Those antigens which could be isolated using immunoadsorbents prepared from the monoclonal antibodies had mol. wts of 42,000, 90,000 and 120,000. The 42,000...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90094-4
更新日期：1984-01-01 00:00:00
abstract：:The effect of fragment B of staphylococcal protein A on the binding of rabbit IgG to human granulocytes has been examined. When rabbit IgG sensitized sheep erythrocytes (EA) were preincubated with increasing amounts of fragment B, a dose dependent inhibition of rosette formation between the human granulocytes and the ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90206-1
更新日期：1982-03-01 00:00:00
abstract：:Recent studies demonstrate that tissue microenvironments may regulate development and functions of immune cells including dendritic cells, monocytes and T cells. However, there is no report about functional regulation of innate NK cells by tissue microenvironment. The liver is an organ abundant in NK cells and suscept...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.01.035
更新日期：2008-05-01 00:00:00
abstract：:Artificial Salmonella serogroup B, D or Cl-specific glycolipids were prepared by covalently linking oligosaccharides corresponding to two O-antigen repeating units, obtained by phage enzyme hydrolysis of native O-antigenic polysaccharides, to octyl residues. Sheep erythrocytes coated with the artificial glycolipids we...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90152-p
更新日期：1990-09-01 00:00:00
