The human antibody repertoire: heavy and light chain variable region gene usage in six alloantibodies specific for human HLA class I and class II alloantigens.

Abstract:

:Peripheral blood B lymphocytes have been isolated from healthy individuals who were immunized with lymphocytes from HLA-incompatible donors and transformed with Epstein-Barr virus to produce human monoclonal cell lines specific for human HLA molecules. The cell lines have been previously characterized and are known to bind to various class I and class II alloantigens. In this report we describe the molecular characterization of the heavy and light chain variable region gene segments that are utilized by these monoclonal antibodies. Using the polymerase chain reaction and primer pairs specific for the respective constant region and VH or VL family, rearranged variable region gene segments were amplified from cDNA from individual cell lines. Products were then subcloned, sequenced and analysed for gene usage and apparent somatic mutation. The results show that the VH3 gene family predominates in a group of six heavy chains (four out of six) with one VH1 and one VH4 gene segment. The light chain variable region gene family usage is more diverse with 2 V kappa 3, 1 V kappa 1, 2 V lambda 2 and 1 V lambda 3. The extent of apparent somatic mutation is minimal, relative to our previous observations in a group of high affinity human monoclonal antibodies specific for pathogenic organisms.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Andris JS,Abraham SR,Pascual V,Pistillo MP,Mantero S,Ferrara GB,Capra JD

doi

10.1016/0161-5890(95)00071-2

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

1105-22

issue

14-15

eissn

0161-5890

issn

1872-9142

journal_volume

32

pub_type

杂志文章
  • Prevention and control of reciprocal T-B cell diversification: implications for lupus-like autoimmunity.

    abstract::Autoimmunity is fundamentally a continuously evolving process. The autoimmune responses shift, drift and diversify with time not only to other epitopes in the original antigen but also to other related and sometimes to unrelated antigens. We have described a form of immune diversification--reciprocal T-B epitope sprea...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.molimm.2003.11.029

    authors: Singh RR

    更新日期:2004-02-01 00:00:00

  • Transcriptome response following administration of a live bacterial vaccine in Atlantic salmon (Salmo salar).

    abstract::Antibacterial responses have been studied in Atlantic salmon following an acute intra peritoneal injection of a genetically attenuated (aroA(-)) strain of Aeromonas salmonicida known to elicit protective immunity. Three tissues were studied for transcriptional changes, the liver, head kidney and the gill. RNA was coll...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2005.10.007

    authors: Martin SA,Blaney SC,Houlihan DF,Secombes CJ

    更新日期:2006-04-01 00:00:00

  • Mechanisms and functions of IL-17 signaling in renal autoimmune diseases.

    abstract::Immune-mediated glomerular diseases (glomerulonephritis) encompass a heterogeneous collection of diseases that cause inflammation within the glomerulus and other renal compartments with significant morbidity and mortality. In general, CD4+ T cells orchestrate the immune response and play a unique role in autoimmune an...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.molimm.2018.09.005

    authors: Schmidt T,Luebbe J,Paust HJ,Panzer U

    更新日期:2018-12-01 00:00:00

  • Engineering the binding properties of the T cell receptor:peptide:MHC ternary complex that governs T cell activity.

    abstract::The potency of a T cell is determined in large part by two interactions, binding of a cognate peptide to the MHC, and binding of the T cell receptor (TCR) to this pepMHC. Various studies have attempted to assess the relative importance of these interactions, and to correlate the corresponding binding parameters with t...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2009.06.012

    authors: Bowerman NA,Crofts TS,Chlewicki L,Do P,Baker BM,Christopher Garcia K,Kranz DM

    更新日期:2009-09-01 00:00:00

  • Transient expression of pentameric IgM on the surface of NZB B cells.

    abstract::In contrast to normal B lymphocytes, splenic B cells from autoimmune NZB mice express on their surface greater amounts of IgM than IgD. We have found that the predominant mu chain species present on these cells is derived from pentameric secretory IgM which can be radioiodinated during transient association with the c...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(88)90026-0

    authors: Yuan D,Goldings EA

    更新日期:1988-04-01 00:00:00

  • Of mice and men: The factor H protein family and complement regulation.

    abstract::For decades immunological research has relied, with variable success, on mouse models to investigate diseases and possible therapeutic interventions. With the approval of the first therapeutic antibody targeting complement, called eculizumab, as therapy in paroxysmal nocturnal hemoglobinuria (PNH) and more recently at...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.molimm.2015.03.011

    authors: Pouw RB,Vredevoogd DW,Kuijpers TW,Wouters D

    更新日期:2015-09-01 00:00:00

  • Functional, molecular and structural characterisation of five anti-Brucella LPS mAb.

    abstract::The O-antigen of the gram negative bacteria Brucella is composed of an homopolymer of 4,6-dideoxy-4-formamido-alpha-D-mannopyranosyl (or perosamine). Several mAb interact specifically with only the O-antigen of certain Brucella species. Although, many studies show that this specific recognition results mainly from the...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2003.11.037

    authors: Laurent TC,Mertens P,Dierick JF,Letesson JJ,Lambert C,De Bolle X

    更新日期:2004-03-01 00:00:00

  • Intracellular co-localization of SPLUNC1 protein with nanobacteria in nasopharyngeal carcinoma epithelia HNE1 cells depended on the bactericidal permeability increasing protein domain.

    abstract::Epithelial surfaces constitute natural immunobarriers against environmental threats. These barriers are brimming with fluids that bind, transport, cleave or degrade bacterial cells and their endotoxic by-products. Saliva and the airway surface-lining fluid (ASL) comprise the important fluid constituents. Short palate,...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2005.10.021

    authors: Zhou HD,Li GY,Yang YX,Li XL,Sheng SR,Zhang WL,Zhao J

    更新日期:2006-04-01 00:00:00

  • Immune evasion by acquisition of complement inhibitors: the mould Aspergillus binds both factor H and C4b binding protein.

    abstract::Pathogenic fungi represent a major threat particularly to immunocompromised hosts, leading to severe, and often lethal, systemic opportunistic infections. Although the impaired immune status of the host is clearly the most important factor leading to disease, virulence factors of the fungus also play a role. Factor H ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2007.08.011

    authors: Vogl G,Lesiak I,Jensen DB,Perkhofer S,Eck R,Speth C,Lass-Flörl C,Zipfel PF,Blom AM,Dierich MP,Würzner R

    更新日期:2008-03-01 00:00:00

  • Antigenic determinants reacting with rheumatoid factor: epitopes with different primary sequences share similar conformation.

    abstract::Polyclonal or monoclonal human IgM rheumatoid factors (RF) react with eight antigenic sites on the CH3 IgG domain, four sites on CH2 and two on human beta 2-microglobulin. All 14 of these RF-reactive epitopes are linear 7-11 amino acid peptides with different primary sequence. We questioned whether RF reactivity with ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/s0161-5890(97)00024-2

    authors: Williams RC Jr,Malone CC,Kolaskar AS,Kulkarni-Kale U

    更新日期:1997-05-01 00:00:00

  • Localisation of three epitopes of the env protein of feline immunodeficiency virus.

    abstract::The envelope protein of the feline immunodeficiency virus (FIV) was analyzed using several epitope prediction programs based on profiles of hydrophilicity, antigenicity, and probability of residues to lie on the protein surface. Tentative homologies with the immunodominant epitope sites in simian virus (SIV) or human ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(92)90192-z

    authors: Avrameas A,Guillet JG,Chouchane L,Moraillon A,Sonigo P,Strosberg AD

    更新日期:1992-05-01 00:00:00

  • IFRD1 regulates the asthmatic responses of airway via NF-κB pathway.

    abstract::Asthma is a chronic respiratory disease which is susceptible to children and causes great harm to them. Recently, Interferon-related developmental regulator 1 (IFRD1) was proved to be participant in regulating lung diseases, and its abnormal expression was shown in pathological airway tissues. Our study aimed to demon...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2020.09.010

    authors: Chang M,Zhang Y,Hui Z,Wang D,Guo H

    更新日期:2020-11-01 00:00:00

  • Cimetidine suppresses lung tumor growth in mice through proapoptosis of myeloid-derived suppressor cells.

    abstract::Cimetidine, a histamine type-2 receptor antagonist, is known to inhibit the growth of several tumors in human and animals, however the mechanism of action underlying this effect remains largely unknown. Here, in the mice model of 3LL lung tumor, cimetidine showed significant inhibition of tumor growth. However, an in ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2012.10.035

    authors: Zheng Y,Xu M,Li X,Jia J,Fan K,Lai G

    更新日期:2013-05-01 00:00:00

  • Structural analyses define an additional H-2D region class I antigen that is expressed by a variant mouse strain.

    abstract::The genetic complexity of the H-2D region includes haplotype disparities in apparent gene and product number. To probe the genetic basis of this complexity, the products of two independently derived mouse strains (STU and B10.SAA48) that express Dw3 antigens were compared. Serologic, fluorometric and peptide map compa...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(86)90007-6

    authors: Lee DR,Lillehoj EP,Myers NB,Coligan JE,Hansen TH

    更新日期:1986-10-01 00:00:00

  • The allergen Mus m 1.0102: Cysteine residues and molecular allergology.

    abstract::Mus m 1.0102 is a member of the mouse Major Urinary Protein family, belonging to the Lipocalins superfamily. Major Urinary Proteins (MUPs) are characterized by highly conserved structural motifs. These include a disulphide bond, involved in protein oxidative folding and protein structure stabilization, and a free cyst...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2020.01.022

    authors: Ferrari E,Corsini R,Burastero SE,Tanfani F,Spisni A

    更新日期:2020-04-01 00:00:00

  • DNA immunization via intramuscular and intradermal routes using a gene gun provides different magnitudes and durations on immune response.

    abstract::We investigated the antibody (Ab) and cytotoxic T lymphocyte (CTL) responses to gene gun (GG)-mediated DNA immunization via the intramuscular (i.m.) and intradermal (i.d.) routes. BALB/c mice were immunized five times at weekly intervals with plasmid DNA encoding enhanced green fluorescent protein (EGFP). EGFP product...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/s0161-5890(03)00024-5

    authors: Ito K,Ito K,Shinohara N,Kato S

    更新日期:2003-05-01 00:00:00

  • Immunochemical determinant and serological specificity of Candida krusei.

    abstract::We have investigated the chemical structure of the antigenic determinant of Candida krusei cell-wall mannan. Acetolysis of the mannan, obtained by extraction with alkali and purified as copper complex, gave five oligosaccharides (from mono- to pentasaccharide) and a minor amount of an octasaccharide. Partial acetolysi...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(82)90202-4

    authors: Nishikawa A,Shinoda T,Fukazawa Y

    更新日期:1982-03-01 00:00:00

  • More efficient peptide binding to MHC class II molecules during cathepsin B digestion of Ii than after Ii release.

    abstract::The binding of a T cell-presented peptide to MHC class II alpha,beta chains occurs as a concurrent process with the release of the associated invariant chain (Ii) by cathepsin B. Ii was digested by cathepsin B from solubilized, MHC class II alpha,beta,Ii complexes in the presence of N-hydroxysuccinimidyl-4-azidobenzoa...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(94)90122-8

    authors: Daibata M,Xu M,Humphreys RE,Reyes VE

    更新日期:1994-03-01 00:00:00

  • IRF-1 and p65 mediate upregulation of constitutive HLA-A antigen expression by hepatocellular carcinoma cells.

    abstract::Malignant transformation of hepatocytes is frequently associated with upregulation of HLA-A expression. Currently there is no information available regarding the mechanisms underlying this phenotypic change. We investigated HLA-A expression in 165 paraffin embedded tissues and 21 fresh tissues from liver cancer patien...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2009.03.001

    authors: Shen Y,Xia M,Zhang J,Xu L,Yang J,Chen A,Miao F,Ferrone S,Xie W

    更新日期:2009-06-01 00:00:00

  • Amyotrophic lateral sclerosis: The complement and inflammatory hypothesis.

    abstract::Amyotrophic lateral sclerosis (ALS) is a devastating, neurodegenerative motor neuron disease. The aetiology of ALS remains an enigma which hinders the design of an effective treatment to prevent, postpone, or reverse the pathophysiological changes occurring during the aggressive progression of this disease. During the...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审

    doi:10.1016/j.molimm.2018.06.007

    authors: Kjældgaard AL,Pilely K,Olsen KS,Pedersen SW,Lauritsen AØ,Møller K,Garred P

    更新日期:2018-10-01 00:00:00

  • Cytohesin-1 regulates human blood neutrophil adhesion to endothelial cells through β2 integrin activation.

    abstract::Cytohesin-1 is a guanine nucleotide exchange factor for ADP ribosylation factor 6 (Arf6) in human blood neutrophils and differentiated PLB-985 neutrophil-like cells. Cytohesin-1 regulates adhesion and the transendothelial migration of monocytes, dendritic cells and T lymphocytes through activation of the β2 integrin L...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2011.03.018

    authors: El azreq MA,Bourgoin SG

    更新日期:2011-07-01 00:00:00

  • A monoclonal antibody directed against a synthetic peptide reacts with a cell surface rabbit class I MHC molecule.

    abstract::Monoclonal antibodies were prepared against synthetic peptides synthetized on the basis of nucleotide sequence from cDNA and genomic clones encoding a class I antigen expressed by the rabbit RL-5 cell line. Using a peptide corresponding to positions 61-73 of the N domain, we were able to obtain an hybridoma producing ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(87)90019-8

    authors: Le Guern A,Wetterskog D,Marche PN,Kindt TJ

    更新日期:1987-05-01 00:00:00

  • Identification of a common HLA-A*0201-restricted epitope among SSX family members by mimicking altered peptide ligands strategy.

    abstract::The synovial sarcoma X breakpoint (SSX) gene family contains nine members. The SSX proteins are CT (cancer/testis) antigens and can be expressed in many tumor types. T cell immune response against SSX protein can be detected in tumor patients and mice expressing any SSX. Screening predominant protective epitopes might...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2008.01.014

    authors: He Y,Mao L,Lin Z,Deng Y,Tang Y,Jiang M,Li W,Jia Z,Wang J,Ni B,Wu Y

    更新日期:2008-05-01 00:00:00

  • Non homologous end joining-mediated DNA break repair is impaired in B lymphocytes of aging mice.

    abstract::Aging is an irreversible physiological process characterized by increased risk of diseases, reduced effectiveness of vaccines, and decreased immune responses. One of the most prominent paradigms of aging and age related conditions is the progressive accumulation of un-repaired DNA breaks leading to apoptosis and exhau...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2012.07.001

    authors: Puthiyaveetil AG,Caudell DL

    更新日期:2013-01-01 00:00:00

  • Memory T cells of patients with abdominal aortic aneurysm differentially expressed micro RNAs 21, 92a, 146a, 155, 326 and 663 in response to Helicobacter pylori and Lactobacillus acidophilus.

    abstract::Regarding the role of micro RNAs (miRNA) in the proliferation and differentiation of T cells as well as the controversy around the role of bacteria in the pathogenesis of abdominal aortic aneurysm (AAA), the effects of Helicobacter pylori (Hp) and Lactobacillus acidophilus (La) were investigated in the induction of mi...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2020.11.007

    authors: Kalani M,Hodjati H,Ghoddusi Johari H,Doroudchi M

    更新日期:2021-02-01 00:00:00

  • Frequent occurrence of conserved extended haplotypes (CEHs) in two Caucasian populations.

    abstract::Conserved extended haplotypes (CEHs) are large (>or=1Mb) regions of identical DNA of the major histocompatibility complex (MHC) region of chromosome 6p in unrelated individuals. They are recognized by family studies and constitute nearly half of MHC haplotypes among European Caucasians. We studied 49 Hungarian Caucasi...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2010.03.013

    authors: Szilágyi A,Bánlaki Z,Pozsonyi E,Yunis EJ,Awdeh ZL,Hossó A,Rajczy K,Larsen CE,Fici DA,Alper CA,Füst G

    更新日期:2010-06-01 00:00:00

  • Orexin A prevents degradation of the articular matrixes in human primary chondrocyte culture.

    abstract::Excessive production of pro-inflammatory cytokines such as interleukin-1β (IL-1β) plays a key role in the pathophysiological development of osteoarthritis (OA). Orexin A is an important peptide of hypothalamic origin, which has displayed its multiple biological functions in several chronic diseases via activation of i...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2018.05.012

    authors: Wang D,Ren H,Li C,Liu F,Wang G,Shi F,Sun Y

    更新日期:2018-09-01 00:00:00

  • Fine molecular analysis of the antigenicity of the Androctonus australis hector scorpion neurotoxin II: a new antigenic epitope disclosed by the Pepscan method.

    abstract::A set of 58 overlapping rod-bound peptides was used to map the antigenic reactivity pattern of a 64-residue neurotoxin (AaH II) from the venom of the scorpion Androctonus australis hector. Five anti-toxin rabbit antisera were assayed serially for their capacity to bind to each peptide in the set. Six regions of antige...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/0161-5890(93)90152-2

    authors: Devaux C,Juin M,Mansuelle P,Granier C

    更新日期:1993-08-01 00:00:00

  • Exosomes expressing neuronal autoantigens induced immune response in antibody-positive autoimmune encephalitis.

    abstract::The immunological role of exosomes in autoimmune encephalitis (AE) remains uncharacterized and not examined. In this study we ought to determine whether exosomes are generated in AE and to define the presence of cell surface neuronal autoantigens (autoAgs) in the cargo. Exosomes were isolated from cerebrospinal fluid ...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2020.12.034

    authors: Gu J,Jin T,Li Z,Chen H,Xia H,Xu X,Gui Y

    更新日期:2021-01-11 00:00:00

  • Crystal structure of Drosophila PGRP-SD suggests binding to DAP-type but not lysine-type peptidoglycan.

    abstract::In Drosophila the synthesis of antimicrobial peptides in response to microbial infections is under the control of the Toll and immune deficiency (Imd) signaling pathways. The Toll signaling pathway responds mainly to Gram-positive bacterial and fungal infection while the Imd pathway mediates the response to Gram-negat...

    journal_title:Molecular immunology

    pub_type: 杂志文章

    doi:10.1016/j.molimm.2008.01.015

    authors: Leone P,Bischoff V,Kellenberger C,Hetru C,Royet J,Roussel A

    更新日期:2008-05-01 00:00:00