Abstract:
:To investigate the IL-6/IL-6 receptor system, we obtained five murine monoclonal antibodies against human IL-6, which neutralize its biological activity. We classified them into two groups (Type I mAb and Type II mAb) according to the epitopes they recognized. These two types of antibodies showed no difference in the manner in which they neutralized IL-6 activity, but they differed in the way they inhibited the binding of IL-6 to its receptor. While Type I mAb inhibited IL-6 binding to its receptor completely, Type II mAb inhibited only partially, even at a concn of Type II mAb sufficient to neutralize biological activity. Scatchard plot analysis revealed that in the case of the human myeloma cell line, the U266 cell, which showed two-phase binding of IL-6 to its receptor, the high affinity binding disappeared and the affinity of the low affinity binding decreased in the presence of Type II mAb. These results suggest that Type I mAb neutralizes IL-6 activity by the blocking of IL-6 binding to its receptor directly. In contrast, Type II mAb neutralizes IL-6 activity not by direct blocking of IL-6 binding to its receptor, but by modulating the binding affinity of IL-6 to its receptor, that is, inhibiting the formation of high affinity binding in IL-6 receptor system. This also indicates that the formation of high affinity may be necessary to transduce the IL-6 signal.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Shimamura T,Taki S,Honda H,Yokota M,Ito S,Takahara Ydoi
10.1016/0161-5890(91)90001-zsubject
Has Abstractpub_date
1991-11-01 00:00:00pages
1155-61issue
11eissn
0161-5890issn
1872-9142journal_volume
28pub_type
杂志文章abstract::We have investigated the chemical structure of the antigenic determinant of Candida krusei cell-wall mannan. Acetolysis of the mannan, obtained by extraction with alkali and purified as copper complex, gave five oligosaccharides (from mono- to pentasaccharide) and a minor amount of an octasaccharide. Partial acetolysi...
journal_title:Molecular immunology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2013-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.06.014
更新日期:2013-12-01 00:00:00
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更新日期:2018-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(83)90139-6
更新日期:1983-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(86)90164-1
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doi:10.1016/j.molimm.2005.02.017
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1996-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:1997-04-01 00:00:00
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