当前位置： SCI文献检索 > MOLECULAR IMMUNOLOGY期刊下所有文献 > Mapping and characterization of antigenic epitopes of arginine kinase of Scylla paramamosain.

Mapping and characterization of antigenic epitopes of arginine kinase of Scylla paramamosain.

Abstract：

:Arginine kinase (AK) is a panallergen present in crustaceans, which can induce an immunoglobulin (Ig) E-mediated immune response in humans. The aim of this work was to map and characterize the antigenic epitopes of Scylla paramamosain AK. Specific-protein-A-enriched IgG raised in rabbits against purified S. paramamosain AK was used to screen a phage display random peptide library. Five AK mimotope clones were identified among 20 random clones after biopanning. Four conformational epitopes D3A4K43M1A5T49T44I7, L31K33V35T32E11E18F14S34D37, V177G172M173D176Q178T174L181K175L187, and R202L170Y203E190P205W204L187T206Y145 were identified with the program LocaPep, and mapped to S. paramamosain AK. The key amino acids of these conformational epitopes were D3, K33, T174, and W204, respectively. On the basis of biopanning, six IgE-specific peptides were mapped with synthetic overlapping peptides using the sera from crab-allergic patients, and four seropositive peptides (amino acids 113-127, 127-141, 141-155, and 204-218) were confirmed as linear epitopes in a degranulation assay in RBL-2H3 cells. Stability experiments showed that the structural integrity of AK is essential for its allergenicity, and the intramolecular disulfide bond at Cys201-Cys271 is essential for its structural stability.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Yang Y,Cao MJ,Alcocer M,Liu QM,Fei DX,Mao HY,Liu GM

doi

10.1016/j.molimm.2015.02.010

subject

Has Abstract

pub_date

2015-06-01 00:00:00

pages

310-20

issue

2

eissn

0161-5890

issn

1872-9142

pii

S0161-5890(15)00050-4

journal_volume

65

pub_type

临床试验,杂志文章

相关文献

MOLECULAR IMMUNOLOGY文献大全
  • C3a receptor deficiency accelerates the onset of renal injury in the MRL/lpr mouse.

    abstract：:The development and progression of systemic lupus erythematosus (SLE) is strongly associated with complement activation and deposition. The anaphylatoxin C3a is a product of complement activation with immunomodulatory properties, and the receptor for C3a (C3aR) is not only expressed by granulocytes and antigen present...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2008.12.004

    authors： Wenderfer SE,Wang H,Ke B,Wetsel RA,Braun MC

    更新日期：2009-04-01 00:00:00

  • 1,25(OH)2D3 induces regulatory T cell differentiation by influencing the VDR/PLC-γ1/TGF-β1/pathway.

    abstract：:Vitamin D has been recommended as an immune modulator in recent years, in addition to regulating calcium-phosphorous-bone metabolism. Clinical studies on organ transplantation found that vitamin D sufficiency patients were less likely to develop acute cellular rejection within one year after transplantation compared t...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2017.09.006

    authors： Zhou Q,Qin S,Zhang J,Zhon L,Pen Z,Xing T

    更新日期：2017-11-01 00:00:00

  • Structural comparison of murine T-cell (B151K12)-derived T-cell-replacing factor (IL-5) with rIL-5: dimer formation is essential for the expression of biological activity.

    abstract：:T-cell-replacing factor (TRF)/IL-5 is a T-cell-derived glycoprotein which has pleiotropic activity on lymphoid and myeloid cells. IL-5 polypeptide translated into Xenopus oocytes are heterogeneous in molecular size (40,000 to 60,000 under nonreducing conditions) and yields a monomeric form (Mr of 25,000 to 30,000) und...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(90)90158-v

    authors： Takahashi T,Yamaguchi N,Mita S,Yamaguchi Y,Suda T,Tominaga A,Kikuchi Y,Miura Y,Takatsu K

    更新日期：1990-09-01 00:00:00

  • Divergent roles of histone deacetylase 6 (HDAC6) and histone deacetylase 11 (HDAC11) on the transcriptional regulation of IL10 in antigen presenting cells.

    abstract：:The anti-inflammatory cytokine IL-10 is a key modulator of immune responses. A better understanding of the regulation of this cytokine offers the possibility of tipping the balance of the immune response toward either tolerance, or enhanced immune responses. Histone deacetylases (HDACs) have been widely described as n...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2014.02.019

    authors： Cheng F,Lienlaf M,Perez-Villarroel P,Wang HW,Lee C,Woan K,Woods D,Knox T,Bergman J,Pinilla-Ibarz J,Kozikowski A,Seto E,Sotomayor EM,Villagra A

    更新日期：2014-07-01 00:00:00

  • High molecular weight components containing N-linked oligosaccharides of Ascaris suum extract inhibit the dendritic cells activation through DC-SIGN and MR.

    abstract：:Helminths, as well as their secretory/excretory products, induce a tolerogenic immune microenvironment. High molecular weight components (PI) from Ascaris suum extract down-modulate the immune response against ovalbumin (OVA). The PI exerts direct effect on dendritic cells (DCs) independent of TLR 2, 4 and MyD88 molec...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2017.03.015

    authors： Favoretto BC,Casabuono AAC,Portes-Junior JA,Jacysyn JF,Couto AS,Faquim-Mauro EL

    更新日期：2017-07-01 00:00:00

  • Frequent occurrence of conserved extended haplotypes (CEHs) in two Caucasian populations.

    abstract：:Conserved extended haplotypes (CEHs) are large (>or=1Mb) regions of identical DNA of the major histocompatibility complex (MHC) region of chromosome 6p in unrelated individuals. They are recognized by family studies and constitute nearly half of MHC haplotypes among European Caucasians. We studied 49 Hungarian Caucasi...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2010.03.013

    authors： Szilágyi A,Bánlaki Z,Pozsonyi E,Yunis EJ,Awdeh ZL,Hossó A,Rajczy K,Larsen CE,Fici DA,Alper CA,Füst G

    更新日期：2010-06-01 00:00:00

  • Biochemical characterization of CD26 (dipeptidyl peptidase IV): functional comparison of distinct epitopes recognized by various anti-CD26 monoclonal antibodies.

    abstract：:In this paper, we performed further biochemical characterization of the CD26 antigen, as defined by the mAbs in anti-1F7 and anti-Ta1, in order to clarify the observed functional differences among these mAbs. For this purpose, we developed a mAb, anti-5F8, which recognizes yet another epitope on the CD26 antigen diffe...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(92)90099-j

    authors： Torimoto Y,Dang NH,Tanaka T,Prado C,Schlossman SF,Morimoto C

    更新日期：1992-02-01 00:00:00

  • Hereditary C1 inhibitor deficiency is associated with high spontaneous amidase activity.

    abstract：BACKGROUND:Angioedema diagnosis classically targets the complement system (via C1 inhibitor (C1Inh) function and antigenic C4 level) and contact phase activation (via amidase activity). Bradykinin is responsible for angioedema attacks and is produced from contact phase activation secondary to failed C1Inh control. OBJ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2017.01.028

    authors： Charignon D,Ghannam A,Ponard D,Drouet C

    更新日期：2017-05-01 00:00:00

  • Role of MAIT cells in metabolic diseases.

    abstract：:MAIT cells are innate-like T cells that are enriched in mucosal sites and tissues including adipose tissue and liver. They play an important role in immunity against microbial pathogens. Recently, it has been reported that MAIT cells could also be important in metabolic diseases and can be involved in setting up and m...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2020.12.014

    authors： Toubal A,Lehuen A

    更新日期：2021-02-01 00:00:00

  • H-2K molecules have two different C-termini, one of which is K-region specific.

    abstract：:Amino acid sequences encoded by exon 8 of the H-2K and H-2D/L genes appear to be locus specific. The majority of H-2Kb molecules contain 10 amino acids that are derived from exon 8. In contrast, the H-2Db, -Dd and -Ld molecules contain only one amino acid which is encoded by exon 8, even though the genetic information...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(88)90165-4

    authors： Maloy WL,Lew AM,Anderson RW,Coligan JE

    更新日期：1988-05-01 00:00:00

  • Effects of surfactant protein D on growth, adhesion and epithelial invasion of intestinal Gram-negative bacteria.

    abstract：:Surfactant protein D (SP-D) interacts with various different microorganisms and plays an important role in pulmonary innate immunity. SP-D expression has also been detected in extrapulmonary tissues, including the gastro-intestinal tract. However, its function in the intestine is unknown and may differ considerably fr...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2007.03.013

    authors： Hogenkamp A,Herías MV,Tooten PC,Veldhuizen EJ,Haagsman HP

    更新日期：2007-07-01 00:00:00

  • Serum levels of IgA1 and IgA2 in children and in patients with IgA deficiency.

    abstract：:Serum levels of IgA1 and IgA2 were measured by solid phase radioimmunoassay in samples from 110 children between 3 months and 10 years of age. Both IgA1 and IgA2 were detectable in all samples, and both IgA1 and IgA2 increased with increasing age. The percent of total serum IgA that was IgA2 did not change with age an...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(83)90038-x

    authors： Conley ME,Arbeter A,Douglas SD

    更新日期：1983-09-01 00:00:00

  • Cimetidine suppresses lung tumor growth in mice through proapoptosis of myeloid-derived suppressor cells.

    abstract：:Cimetidine, a histamine type-2 receptor antagonist, is known to inhibit the growth of several tumors in human and animals, however the mechanism of action underlying this effect remains largely unknown. Here, in the mice model of 3LL lung tumor, cimetidine showed significant inhibition of tumor growth. However, an in ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2012.10.035

    authors： Zheng Y,Xu M,Li X,Jia J,Fan K,Lai G

    更新日期：2013-05-01 00:00:00

  • Porcine deltacoronavirus nsp15 antagonizes interferon-β production independently of its endoribonuclease activity.

    abstract：:Porcine deltacoronavirus (PDCoV) is an emerging swine coronavirus causing diarrhea and intestinal damage in nursing piglets. Previous work showed that PDCoV infection inhibits type I interferon (IFN) production. To further identify and characterize the PDCoV-encoded IFN antagonists will broaden our understanding of it...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2019.07.003

    authors： Liu X,Fang P,Fang L,Hong Y,Zhu X,Wang D,Peng G,Xiao S

    更新日期：2019-10-01 00:00:00

  • Increased expression of MHC class I molecules on human cells after short time IFN-gamma treatment.

    abstract：:Human cell lines and blood lymphocytes were treated for short time periods with IFN-gamma. This treatment increased the amount of the assembled MHC class I molecules on the plasma membrane after 30 min. This early increase of the membrane expression subsided in the next few hours. A second wave of elevation occurred a...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(93)90008-y

    authors： Végh Z,Wang P,Vánky F,Klein E

    更新日期：1993-06-01 00:00:00

  • The Fli-1 transcription factor is a critical regulator for controlling the expression of chemokine C-X-C motif ligand 2 (CXCL2).

    abstract：:Mammalian cells produce inflammatory cytokines and chemokines in response to innate immune signals and their expression is tightly regulated. Chemokine (C-X-C motif) ligand 2 (CXCL2), also known as macrophage inflammatory protein 2-alpha (MIP2-alpha), is an inflammatory chemokine belonging to the CXC chemokine family....

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2016.11.007

    authors： Lou N,Lennard Richard ML,Yu J,Kindy M,Zhang XK

    更新日期：2017-01-01 00:00:00

  • The mouse genome contains two expressed intronless retroposed pseudogenes for the sentrin/sumo-1/PIC1 conjugating enzyme Ubc9.

    abstract：:The ubiquitin conjugating (ubc) E2 enzyme ubc-9 conjugates the ubiquitin-like peptide sentrin/SUMO-1/PIC1 to target proteins which include the Fas antigen. We show that the mouse genome contains four copies of the ubc-9 gene. These include a structural ubc-9 gene consisting of seven exons which encode a protein identi...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/s0161-5890(98)00094-7

    authors： Tsytsykova AV,Tsitsikov EN,Wright DA,Futcher B,Geha RS

    更新日期：1998-11-01 00:00:00

  • PECAM-1-regulated signalling thresholds control tolerance in anergic transgenic B-cells.

    abstract：:Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1/CD31) is an immunoglobulin (Ig)-immunoreceptor tyrosine based inhibitory motif (Ig-ITIM) superfamily member that recruits and activates protein-tyrosine phosphatases, predominantly SHP-2 and to a lesser extent, SHP-1. Previously, we have shown that deletion of PEC...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2007.09.024

    authors： Wong MX,Hayball JD,Jackson DE

    更新日期：2008-03-01 00:00:00

  • Cloning of a cDNA for a T cell produced molecule with a putative immune regulatory role.

    abstract：:An expression cDNA library was constructed from the helper T cell hybridoma, A.1.1, which has been shown to produce constitutively proteins involved in the down regulation of the immune response. From this library we identified and characterized a cDNA clone, J6B7, by screening with a polyclonal antibody specific for ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(90)90102-6

    authors： Lee C,Ghoshal K,Beaman KD

    更新日期：1990-11-01 00:00:00

  • Potentiation of factor H by heparin: a rate-limiting mechanism for inhibition of the alternative complement pathway.

    abstract：:The mechanism by which heparin inhibits the alternative complement pathway (ACP) by a fluid-phase activator, CoVF, has been studied. Results presented here indicate that heparin's major (rate-limiting) effect on the fluid-phase activation of the ACP was to potentiate Factor H activity. Such an effect results in a very...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(83)90139-6

    authors： Boackle RJ,Caughman GB,Vesely J,Medgyesi G,Fudenberg HH

    更新日期：1983-11-01 00:00:00

  • Comparative molecular modelling of the Fas-ligand and other members of the TNF family.

    abstract：:A number of proteins with significant similarity to the tumour necrosis factor (TNF) have been identified over the last years. Upon interaction with their cognate receptor (members of the TNF-receptor family), all members of this protein family induce either cell death or proliferation/differentiation of the receptor-...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(95)00016-8

    authors： Peitsch MC,Tschopp J

    更新日期：1995-07-01 00:00:00

  • Preventive and therapeutic oral administration of the pentacyclic triterpene α,β-amyrin ameliorates dextran sulfate sodium-induced colitis in mice: the relevance of cannabinoid system.

    abstract：:The pentacyclic triterpene α,β-amyrin has been previously reported as an effective compound in the treatment of several inflammatory conditions. Recent evidence indicates that α,β-amyrin displayed its effects through interaction with the cannabinoid pathway. We assessed the anti-inflammatory effects of the α,β-amyrin ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2013.01.018

    authors： Matos I,Bento AF,Marcon R,Claudino RF,Calixto JB

    更新日期：2013-07-01 00:00:00

  • Potentiated macrophage activation by acid sensing under low adiponectin levels.

    abstract：:Adiponectin can protect against inflammation; one of the mechanisms involves direct, inhibition of macrophages (MΦ). We postulated that adiponectin anti-sense transgenic (AsTg) mice raised in our laboratory are prone to inflammation because of systemic low adiponectin levels. The writhing response to acetic acid was u...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2013.08.015

    authors： Negoro T,Kin M,Takuma A,Saito K,Shimizu S,Nakano Y

    更新日期：2014-02-01 00:00:00

  • A region of the N-terminal domain of meningococcal factor H-binding protein that elicits bactericidal antibody across antigenic variant groups.

    abstract：:Meningococcal factor H-binding protein (fHbp) is a promising vaccine antigen. Previous studies described three fHbp antigenic variant groups and identified amino acid residues between 100 and 255 as important targets of variant-specific bactericidal antibodies. We investigated residues affecting expression of an epito...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2009.02.021

    authors： Beernink PT,LoPasso C,Angiolillo A,Felici F,Granoff D

    更新日期：2009-05-01 00:00:00

  • Differential effect of insulin treatment on decreased levels of beta-defensins and Toll-like receptors in diabetic rats.

    abstract：:Many infections are associated with diabetes, as the ability of the body to fight pathogens is impaired. Recently, altered levels of defensins and polymorphism in Toll-like receptors (TLRs), two crucial components of the innate immune system, have been associated with diabetes. To study the functionality of the innate...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2006.04.009

    authors： Froy O,Hananel A,Chapnik N,Madar Z

    更新日期：2007-02-01 00:00:00

  • SPI-C, a PU-box binding ETS protein expressed temporarily during B-cell development and in macrophages, contains an acidic transactivation domain located to the N-terminus.

    abstract：:Mice deficient for SPI-group ETS transcription factors PU.1 or SPI-B fail to generate lymphocytes or do not mount normal antibody mediated immune responses, respectively. PU.1 expression is restricted to B-, T-lymphocytes and macrophages, while SPI-B is expressed in B- and T-lymphocytes. SPI-C is an ETS transcription ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/s0161-5890(03)00032-4

    authors： Carlsson R,Persson C,Leanderson T

    更新日期：2003-06-01 00:00:00

  • Complement control protein factor H: the good, the bad, and the inadequate.

    abstract：:The complement system is an essential component of the innate immune system that participates in elimination of pathogens and altered host cells and comprises an essential link between the innate and adaptive immune system. Soluble and membrane-bound complement regulators protect cells and tissues from unintended comp...

    journal_title：Molecular immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molimm.2010.05.007

    authors： Ferreira VP,Pangburn MK,Cortés C

    更新日期：2010-08-01 00:00:00

  • Bradykinin-induced IL-6 expression through bradykinin B2 receptor, phospholipase C, protein kinase Cdelta and NF-kappaB pathway in human synovial fibroblasts.

    abstract：:Bradykinin (BK) is an inflammatory mediator, and shows elevated levels in regions of severe injury and inflammatory diseases. It has been shown to induce interleukin-6 (IL-6) expression in inflammatory responses in rheumatoid arthritis. We investigated the signaling pathway involved in IL-6 production caused by BK in ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2008.06.007

    authors： Lee CH,Shieh DC,Tzeng CY,Chen CP,Wang SP,Chiu YC,Huang CY,Hsu CJ,Fong YC,Tang CH

    更新日期：2008-08-01 00:00:00

  • Isolation of a protein labeled with diisopropyl fluorophosphate on stimulation of polymorphonuclear leukocytes with immune complexes.

    abstract：:As demonstrated by others, diisopropyl fluorophosphate (DFP) markedly inhibits the O2- generation from guinea-pig polymorphonuclear leukocytes (PMN) stimulated by an antibody complex with ovalbumin (Ag-Ab complex), and also the intracellular uptake of antibody-sensitized erythrocytes by the cells. However, when PMN we...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(85)90113-0

    authors： Kudoh M,Nakamura T,Koyama J

    更新日期：1985-09-01 00:00:00

  • Single amino acid substitutions in V(H) CDR2 are sufficient to generate or enhance the specificity of two forms of an anti-arsonate antibody variable region for DNA.

    abstract：:We previously showed that a variety of amino acid substitutions at positions 58 and 59 in the V(H) CDR2 of an anti-arsonate (Ars) antibody Fab simultaneously resulted in increased or unaltered affinity for Ars and substantially enhanced affinity for DNA. To test the generality of these observations, we generated and c...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/s0161-5890(98)00005-4

    authors： Hande S,Manser T

    更新日期：1997-12-01 00:00:00