Abstract:
:In this paper, we performed further biochemical characterization of the CD26 antigen, as defined by the mAbs in anti-1F7 and anti-Ta1, in order to clarify the observed functional differences among these mAbs. For this purpose, we developed a mAb, anti-5F8, which recognizes yet another epitope on the CD26 antigen different from that recognized by anti-1F7 and anti-Ta1 and compared their respective effect on T cell activation as well as the structures recognized by these mAbs. Functionally, anti-5F8 did not exhibit a comitogenic effect on T cell activation via the CD3 and CD2 pathways. Peptide mapping studies suggested that the 110 kDa molecules precipitated by these mAbs are identical. We showed that the 110 kDa CD26 structure on human T cells is composed of a family of heterogeneous molecules, as determined by isoelectric focusing studies. In addition, we demonstrated that the CD26 antigen has a DPPIV enzyme activity and this enzyme activity is found only on the principal basic structure of CD26 but not on the additional acidic structures. Biochemical studies also revealed that these mAbs recognized distinct epitopes on the CD26 antigen. Pulse-chase studies showed the the 1F7 epitope was found on both the immature (100 kDa) and mature (110 kDa) forms of the CD26 antigen. On the other hand, the Ta1 and 5F8 epitopes were expressed mainly on the mature form of the CD26 antigen. Moreover, anti-IF7 consistently precipitated an additional 43 kDa molecule in association with the principal 110 kDa molecule. Taken together, these data suggested that the additional 43 kDa structure or the distinct epitope recognized by anti-IF7 may play a role in human T cell activation via the CD3 and CD2 pathways.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Torimoto Y,Dang NH,Tanaka T,Prado C,Schlossman SF,Morimoto Cdoi
10.1016/0161-5890(92)90099-jsubject
Has Abstractpub_date
1992-02-01 00:00:00pages
183-92issue
2eissn
0161-5890issn
1872-9142journal_volume
29pub_type
杂志文章abstract::Four cell lines producing monoclonal antibodies were obtained by fusion of NS1 myeloma cells with splenocytes of BALB/C mice immunized with only 1 microgram of each staphylococcal enterotoxin A, B, C1 and D by a modified technique of intrasplenic boosting. This procedure was considerably more efficient than the more c...
journal_title:Molecular immunology
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journal_title:Molecular immunology
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90061-2
更新日期:1988-02-01 00:00:00
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journal_title:Molecular immunology
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.01.009
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journal_title:Molecular immunology
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2018.07.013
更新日期:2018-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.02.002
更新日期:2007-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.11.018
更新日期:2018-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90111-9
更新日期:1989-10-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90194-3
更新日期:1992-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.09.024
更新日期:2009-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(88)90159-9
更新日期:1988-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90125-u
更新日期:1993-08-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(03)00100-7
更新日期:2003-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.07.001
更新日期:2013-01-01 00:00:00
abstract::C1q, the recognition molecule of the classical pathway of complement, binds to endothelial cells, leading to cell activation. Mannose-binding lectin (MBL), a recognition molecule of the lectin pathway, is structurally and functionally related to C1q. Therefore, we investigated the interaction of MBL with human umbilic...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.06.015
更新日期:2007-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(98)00077-7
更新日期:1998-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(98)00094-7
更新日期:1998-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.02.017
更新日期:2007-01-01 00:00:00
abstract::Although the etiology of rheumatoid arthritis (RA) remains unknown, it has been widely suggested that RA has a genetic background. In humans, a copy number loss of 22q11.2, a region harboring the VPREB1 gene, has been suggested to be associated with several immunologic disorders, but there has been no study on the cop...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.11.009
更新日期:2011-06-01 00:00:00
abstract::An important function of Fcγ receptors is the removal of IgG-containing immune complexes from the circulation. The activating receptor FcγRIIA and inhibitory receptor FcγRIIB2 are both expressed on human myeloid cells, and are both capable of mediating endocytosis of immune complexes. We studied endocytosis of these t...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.09.003
更新日期:2011-10-01 00:00:00
abstract::GK-1 is an immunomodulatory, 18-aa-long peptide that has been proved to promote the activation of mouse peritoneal macrophages and LPS-pulsed mouse bone marrow-derived dendritic cells (BM-DCs). This study is aimed to explore the mechanisms underlying the activation of these antigen-presenting cells (APCs) by GK-1. In ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.09.015
更新日期:2020-12-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2010.06.003
更新日期:2010-09-01 00:00:00
abstract::A highly purified preparation of a monoclonal antibody to vasoactive intestinal peptide (VIP) was analysed by gel filtration. Three peaks of VIP hydrolysing activity were observed, corresponding to the 150 kDa tetramer IgG, 80 kDa dimer of the heavy and light chains (H-L dimer) and 25 kDa L chain monomer. The hydrolyt...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(96)00021-1
更新日期:1996-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.02.018
更新日期:2010-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(02)00080-9
更新日期:2002-09-01 00:00:00
abstract::The mammalian interleukin (IL)-6 family of cytokines consist of eight class-I helical cytokines and are major players in hematopoiesis, as well as in neuroendocrine and immune systems, and have pro- and anti-inflammatory properties. We have identified a novel fish CNTF-like molecule, for the first time, as well as the...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.04.003
更新日期:2009-07-01 00:00:00
abstract::The complement system plays a central role in a number of human inflammatory diseases, and there is a significant need for development of complement-directed therapies. The discovery of an arsenal of anti-complement proteins secreted by the pathogen Staphylococcus aureus brought with it the potential for harnessing th...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2015.05.012
更新日期:2015-10-01 00:00:00