Abstract：
:A recent report indicated that T200 molecules interact with elements of the cytoskeleton in BW5147 T lymphoma cells. We have confirmed the cytoskeletal association of T200 by examining nonionic detergent-soluble and detergent-insoluble fractions of murine T cell tumor cell lines, cloned cytotoxic T lymphocyte lines, and thymocytes. Concanavalin A (Con A)-treated and untreated cells were extracted with 0.5% Triton X-100 and the remaining insoluble material was extracted under conditions allowing actin depolymerization. In the absence of Con A treatment, little T200 could be recovered from the depolymerized insoluble fraction. However, in T cells treated with capping concentrations of Con A, a considerable amount of T200 was rendered insoluble in nonionic detergent, and T200 could be recovered from the insoluble fraction by a buffer which dissociates actin polymers. A lesser, but still significant, amount of T200 associated with the detergent-insoluble fraction of thymocytes treated with concentrations of Con A and succinyl Con A, which are mitogenic for T cells. We also found that in T cells treated with mitogenic concentrations of succinyl Con A, more T200 associated with cytoskeleton than did H-2 or LFA-1 molecules. Because T200 is such a predominant molecule on the surface of T cells, such translocations of the molecule may have a major impact on the physiology of the cell, especially if T200 functions as a protein tyrosine phosphatase as recent evidence by others suggests.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Taffs RE,Ewald SJdoi
10.1016/0161-5890(89)90111-9subject
Has Abstractpub_date
1989-10-01 00:00:00pages
925-37issue
10eissn
0161-5890issn
1872-9142journal_volume
26pub_type
杂志文章abstract：:An efficient method is described for the isolation of highly purified, IgG-free and stable guinea-pig serum C1q. The procedure includes the chromatography of EDTA-treated serum (25 mM EDTA) on CM- and DEAE-cellulose followed by gel filtration on ACA 34-Ultrogel whereby ammonium sulfate precipitation was used for conce...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90133-5
更新日期：1983-02-01 00:00:00
abstract：:Antigens related to the carcinoembryonic antigen (CEA) were isolated from normal human plasma by perchloric acid extraction, gel permeation chromatography and immunoaffinity chromatography using a monoclonal antibody with broad specificity and high affinity. The antigens were separated by SDS-polyacrylamide gel electr...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90046-x
更新日期：1985-11-01 00:00:00
abstract：:Covalent Superose microspheres-bound C3b was used as a model system to simplify the analysis of antigen-bound C3b modifications during antigen processing. The model was set up using purified C3 and Superose-bound trypsin. C3b was covalently bound to Superose through an ester link, as indicated by lability to hydroxyla...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90009-z
更新日期：1993-07-01 00:00:00
abstract：:In the previous study, antibodies against CD3 molecule have been produced and were used in labeling T cells in Japanese flounder (Paralichthys olivaceus). In this paper, CD4+ and CD8+ lymphocytes subpopulations in peripheral blood leucocytes (PBL), spleen and head kidney of flounder were investigated. The flounder CD4...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.02.015
更新日期：2017-05-01 00:00:00
abstract：:In addition to classic Smad signaling pathways, the pleiotropic immunoregulatory cytokine TGF-beta1 can activate MAP kinases, but a role for TGF-beta1-MAP kinase pathways in T cells has not been defined heretofore. We have shown previously that TGF-beta1 inhibits Th1 development by inhibiting IFN-gamma's induction of ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.02.024
更新日期：2007-07-01 00:00:00
abstract：BACKGROUND:Atopic dermatitis (AD) or atopic eczema is a skin disease characterized with itching, increased serum level of immunoglobulin E, and peripheral eosinophilia. Thymoquinone (TQ) is an important ingredient of Nigella sativa seeds having antioxidant and anti-inflammatory effects. OBJECTIVE:Present study investi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.07.013
更新日期：2018-09-01 00:00:00
abstract：:Interleukin 1β (IL-1β) is a pro-inflammatory cytokine secreted by activated macrophages and monocytes. Previously, we have reported that bone morphogenetic protein-6 (BMP-6) induces inducible nitric oxide synthase (iNOS) expression via IL-1β in macrophages. In the present study, we demonstrate that BMP-6 increases IL-...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.04.019
更新日期：2011-07-01 00:00:00
abstract：:Pathogenic micro-organisms employ a broad range of strategies to survive in and to persistently infect the human host. Far from being completely understood by which highly sophisticated means invading pathogens overcome the host's destructive immune defence, there is a growing body of evidence on particular mechanisms...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2005.06.016
更新日期：2006-01-01 00:00:00
abstract：:Excessive production of pro-inflammatory cytokines such as interleukin-1β (IL-1β) plays a key role in the pathophysiological development of osteoarthritis (OA). Orexin A is an important peptide of hypothalamic origin, which has displayed its multiple biological functions in several chronic diseases via activation of i...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.05.012
更新日期：2018-09-01 00:00:00
abstract：:The fifth component of human complement (C5), under physiological salt conditions, bound firmly to phenyl-Sepharose. This indicated the presence of hydrophobic sites on C5. These sites may play an important role in C5 haemolytic activity since this activity was inhibited by chaotropic ions (Mg2+, SCN-, I-) at relative...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90031-4
更新日期：1988-04-01 00:00:00
abstract：:Leucine-rich repeat-containing G-protein-coupled receptors (LGRs) form a subfamily of the large superfamily of G-protein-coupled receptors. LGRs can be divided into three groups. LGR2 from Drosophila melanogaster is involved in cuticle tanning (melanization and sclerotization). In this study, one LGR2 (MnLGR2) was ide...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.07.013
更新日期：2020-10-01 00:00:00
abstract：:BST-2 (also known as tetherin, CD317, or HM1.24) was first described as a potent interferon-inducible host antiviral factor nearly five years ago. Since that time, numerous reports have been published regarding the antiviral activity and immunological properties of this protein. BST-2 blocks viral replication by inhib...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2012.11.008
更新日期：2013-06-01 00:00:00
abstract：:Interleukin-12 (IL-12) is a heterodimeric cytokine produced by macrophages in response to intracellular pathogens and provides an obligatory signal for the differentiation of T-helper-1 cells. p300 is an important histone acetyltransferase (HAT) and has been implicated in the regulation of gene expression. Whether p30...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.05.013
更新日期：2004-11-01 00:00:00
abstract：:1,25-Dihydroxyvitamin D3 (1,25-D3) is known to have potent inhibitory effects on human peripheral blood mononuclear cell (PBMC) functions. Previous experiments suggest that addition of interleukin-2 (IL-2) to cell cultures can reverse the antiproliferative action of 1,25-D3. Previous studies have also shown that the C...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90064-7
更新日期：1990-01-01 00:00:00
abstract：:Cathepsin L protease belongs to the papain-like cysteine proteases family, plays indispensable roles in animals' pathological and physiological processes. However, little is known about Cathepsin L in silkworm, Bombyx mori. Herein, a novel Cathepsin L-like (Cat L-like) was cloned and identified from silkworm by the ra...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.12.013
更新日期：2020-12-26 00:00:00
abstract：:Amyloid-beta (Aβ) plaque accumulation in the brain is one of the hallmarks of Alzheimer's disease (AD). Immunotherapy against Aβ was considered a potential strategy for reducing the Aβ load in the brain. However, none of the Aβ immunotherapies have produced clinically meaningful results to date, due to poor safety or ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.07.019
更新日期：2018-09-01 00:00:00
abstract：:We investigated the antibody (Ab) and cytotoxic T lymphocyte (CTL) responses to gene gun (GG)-mediated DNA immunization via the intramuscular (i.m.) and intradermal (i.d.) routes. BALB/c mice were immunized five times at weekly intervals with plasmid DNA encoding enhanced green fluorescent protein (EGFP). EGFP product...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(03)00024-5
更新日期：2003-05-01 00:00:00
abstract：:Systemic lupus erythematosus (SLE) is a typical autoimmune disease with unclear etiology. Aberrant self-DNA recognition has been proven critical for the initiation of excessive immune responses in lupus. Consistently, we previously reported that a sort of self-DNA, activated lymphocyte-derived DNA (ALD-DNA) could pote...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.01.023
更新日期：2015-05-01 00:00:00
abstract：:Macrophages play an important role both in innate and adaptive immune responses. Treatment with interferon (IFN) γ together with lipopolysaccharide (LPS) activates pro-inflammatory macrophages which secrete various pro-inflammatory cytokines including IL-12. IL-12 promotes a Th1 type immune response by directly contro...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.03.017
更新日期：2012-07-01 00:00:00
abstract：:Complement factor H (CFH) protein is an inhibitor of the alternative pathway of complement (AP) both in the fluid phase and on the surface of host cells. Mouse and human complement factor H-related (CFHR) proteins also belong to the fH family of plasma glycoproteins. The main goal of the current study was to compare t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.06.028
更新日期：2014-11-01 00:00:00
abstract：:Neutrophil migration is essential for battling against infections but also drives chronic inflammation. Since primary neutrophils are terminally differentiated and not genetically tractable, leukemia cells such as HL-60 are differentiated into neutrophil-like cells to study mechanisms underlying neutrophil migration. ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.06.001
更新日期：2019-08-01 00:00:00
abstract：:Notexin and nigexine are monomeric phospholipases A2(PLA2s) from the venoms of Notechis scutatus scutatus and Naja nigricollis, respectively. Polyclonal antibodies raised in mice against these antigenic proteins displayed non-reciprocal cross-reactivity; anti-notexin antibodies recognized notexin but not nigexine, whe...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90055-5
更新日期：1990-01-01 00:00:00
abstract：:Sepsis is a life-threatening disease characterized by uncontrolled inflammatory responses upon pathogen infections, especially for the antibiotic-resistant strains, such as Methicillin-resistant S. aureus (MRSA). Here we demonstrated that a Mitochondria-derived peptide (MOTS-c) could significantly improve the survival...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.10.017
更新日期：2017-12-01 00:00:00
abstract：:The three-dimensional structure of the Mcg lambda-type Bence-Jones dimer crystallized in ammonium sulfate is known at 2.3-A resolution. A series of nine other human lambda-chains and two kappa-chains did not crystallize under the same conditions. After these proteins were hybridized with the Mcg light chain by the met...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(85)80001-8
更新日期：1985-02-01 00:00:00
abstract：:The sphingosine-1-phosphate analogue FTY720 is known to alter migration and homing of lymphocytes via sphingosine-1-phosphate receptors. However, several studies indicate that its mode of action is more complex and that FTY720 may also directly influence cytokine effector functions. Therefore, we studied the effect of...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.02.026
更新日期：2007-07-01 00:00:00
abstract：:An attempt was made to obtain fragments containing the C gamma 2 region by selectively cleaving human Fc fragments prepared from pooled IgG at Met residues using cyanogen bromide. Based on the known locations of Met residues in the Fc regions of human IgG subclasses, fragments between Met 252 and 358, comprising the C...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90052-5
更新日期：1985-12-01 00:00:00
abstract：:Arginine kinase (AK) is a panallergen present in crustaceans, which can induce an immunoglobulin (Ig) E-mediated immune response in humans. The aim of this work was to map and characterize the antigenic epitopes of Scylla paramamosain AK. Specific-protein-A-enriched IgG raised in rabbits against purified S. paramamosa...
journal_title：Molecular immunology
pub_type： 临床试验,杂志文章
doi：10.1016/j.molimm.2015.02.010
更新日期：2015-06-01 00:00:00
abstract：:The effect of antibody affinity on molecular forms of immune complexes was investigated by measuring antigen-antibody interactions using surface plasmon resonance (SPR), electrospray ionization time-of-flight mass spectrometry under non-denaturing conditions (MS), analytical ultracentrifugation (AUC), and transmission...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.09.009
更新日期：2009-12-01 00:00:00
abstract：:Immunoglobulins (Ig) require correct folding and assembly of both heavy (H) and light (L) chains to form a functional H2L2 dimer that is secreted from plasma cells. This process is dependent upon the endoplasmic reticulum (ER) chaperone BiP, which targets improperly, folded or assembled Ig molecules for degradation. W...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.11.015
更新日期：2005-05-01 00:00:00
abstract：:We previously demonstrated that some fish have unique response in the form of reactive oxygen species (ROS) production. In the present study, we cloned and sequenced the full-length cDNAs for carp (Cyprinus carpio) phagocyte NADPH oxidase components: gp91phox, p22phox, p47phox, p67phox and p40phox. These amino acid se...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.09.028
更新日期：2008-03-01 00:00:00
