Abstract:
:Notexin and nigexine are monomeric phospholipases A2(PLA2s) from the venoms of Notechis scutatus scutatus and Naja nigricollis, respectively. Polyclonal antibodies raised in mice against these antigenic proteins displayed non-reciprocal cross-reactivity; anti-notexin antibodies recognized notexin but not nigexine, whereas anti-nigexine antibodies recognized both antigens. Polyclonal antibodies raised by successive immunization with nigexine and notexin contained cross-reacting antibodies with affinities for both antigens that differed from those of antibodies present in anti-nigexine antiserum. A monoclonal antibody has been obtained from a mouse immunized with both PLA2s. This monoclonal antibody, called MN1, recognized notexin and nigexine with comparable high affinity (Kd = 10(-9) M). It also recognized most purified PLA2s from elapid snake venoms and all PLA2-containing venoms from cobras and sea-snakes. This offers the first demonstration that most PLA2s from cobras and sea-snakes share a fine structure which is not restricted to the common catalytic site.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Mollier P,Chwetzoff S,Ménez Adoi
10.1016/0161-5890(90)90055-5subject
Has Abstractpub_date
1990-01-01 00:00:00pages
7-15issue
1eissn
0161-5890issn
1872-9142journal_volume
27pub_type
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journal_title:Molecular immunology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular immunology
pub_type:
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journal_title:Molecular immunology
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(86)90163-x
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pub_type: 杂志文章
doi:10.1016/j.molimm.2009.09.022
更新日期:2009-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(92)90123-f
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pub_type: 杂志文章
doi:10.1016/j.molimm.2020.01.012
更新日期:2020-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(83)90091-3
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journal_title:Molecular immunology
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doi:10.1016/j.molimm.2007.06.151
更新日期:2007-09-01 00:00:00
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(95)00072-m
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(86)90049-0
更新日期:1986-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2004.06.011
更新日期:2004-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2017.05.014
更新日期:2017-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.05.019
更新日期:2016-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:1985-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.08.010
更新日期:2007-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.11.006
更新日期:2014-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90029-9
更新日期:1983-04-01 00:00:00