Differential effect of insulin treatment on decreased levels of beta-defensins and Toll-like receptors in diabetic rats.


:Many infections are associated with diabetes, as the ability of the body to fight pathogens is impaired. Recently, altered levels of defensins and polymorphism in Toll-like receptors (TLRs), two crucial components of the innate immune system, have been associated with diabetes. To study the functionality of the innate immune system during diabetes, we measured the expression levels of rat beta-defensin 1 (rBD-1), rBD-2, rTLR2, and rTLR4 in the kidney, lung, liver, and brain of streptozotocin-induced diabetic rats with and without insulin treatment. In the kidneys of diabetic rats, lower levels of rBD-1, rTLR2, and rTLR4 were found. These low levels could be restored with insulin treatment. In contrast, rBD-2 was highly induced in the lungs of diabetic rats, most likely, as a result of a pro-inflammatory response. In the liver and brain of diabetic rats, lower levels of TLRs and/or defensins could not be restored with insulin treatment. Our findings suggest for the first time that high blood glucose and/or insulin deficiency abrogates the expression level of the innate immune system components, defensins and TLRs, explaining the recurrent infections during diabetes. These findings may lay the grounds for future design of novel treatments that will induce defensins and TLRs and, as a result, bolster the immune system in diabetic patients.


Mol Immunol


Molecular immunology


Froy O,Hananel A,Chapnik N,Madar Z




Has Abstract


2007-02-01 00:00:00














  • Immunochemical determinant and serological specificity of Candida krusei.

    abstract::We have investigated the chemical structure of the antigenic determinant of Candida krusei cell-wall mannan. Acetolysis of the mannan, obtained by extraction with alkali and purified as copper complex, gave five oligosaccharides (from mono- to pentasaccharide) and a minor amount of an octasaccharide. Partial acetolysi...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Nishikawa A,Shinoda T,Fukazawa Y

    更新日期:1982-03-01 00:00:00

  • NCCRP-1: a novel receptor protein sequenced from teleost nonspecific cytotoxic cells.

    abstract::The recognition structure responsible for binding to conventional antigen on target cells has not previously been described for nonspecific cytotoxic cells (NCC) or for mammalian natural killer (NK) cells. Although several biochemical pathways may be available for initiation of the lytic cycle in NCC, evidence present...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Jaso-Friedmann L,Leary JH 3rd,Evans DL

    更新日期:1997-08-01 00:00:00

  • An increase in the number of recombinant molecules and other effects of the simultaneous allotype suppression of trans-chromosomal a VH and n Cmu Ig gene products.

    abstract::The concomitant effects of trans-chromosomal allotype suppression of both an a VH and an n Cmu locus allotype in multiheterozygous rabbits were investigated. For example of the expression of the a2 VH and n81 Cmu allotypes were suppressed in a multiheterozygous rabbit having the a1 chi-y-n81de12,15f73g74/a2 chi 32y33n...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Horng WJ,Roux KH,Gilman-Sachs A,Dray S

    更新日期:1982-01-01 00:00:00

  • Monoclonal anti-histone H1 autoantibodies from MRL lpr/lpr mice.

    abstract::Hybridomas producing anti-histone monoclonal antibodies were generated from a 2-month old MRL lpr/lpr mouse. Five IgM antibodies showed binding to histone H3 and three of these IgM antibodies also bound histone H1. Two IgG2a antibodies (MRA3 and MRA12) were specific for histone H1 and their binding was further charact...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Monestier M,Fasy TM,Böhm L

    更新日期:1989-08-01 00:00:00

  • Evolutionary implication of B-1 lineage cells from innate to adaptive immunity.

    abstract::The paradigm that B cells mainly play a central role in adaptive immunity may have to be reevaluated because B-1 lineage cells have been found to exhibit innate-like functions, such as phagocytic and bactericidal activities. Therefore, the evolutionary connection of B-1 lineage cells between innate and adaptive immuni...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Zhu LY,Shao T,Nie L,Zhu LY,Xiang LX,Shao JZ

    更新日期:2016-01-01 00:00:00

  • Dynamic DNA methylation patterns across the mouse and human IL10 genes during CD4+ T cell activation; influence of IL-27.

    abstract::IL-10 plays a critical role in controlling inflammation and the anti-inflammatory functions of IL-10 are regulated based on its coordinated expression from various cellular sources, most notably T cells. Although nearly all CD4+ subpopulations can express IL-10, surprisingly little is known about the molecular mechani...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Hedrich CM,Ramakrishnan A,Dabitao D,Wang F,Ranatunga D,Bream JH

    更新日期:2010-11-01 00:00:00

  • Variable region cDNA sequences of three mouse monoclonal anti-idiotypic antibodies specific for anti-alpha(1----6)dextrans with groove- or cavity-type combining sites.

    abstract::The variables regions of three syngeneic anti-idiotypic antibodies (Ab2s) were cloned and sequenced. They are encoded by different VL genes, two are from different members of V kappa-Ox1 superfamily. The H chains are encoded by VH genes belonging to three different VH families, J558, Q52 and 7183. Together with a prev...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Chen J,Borden P,Liao J,Kabat EA

    更新日期:1992-09-01 00:00:00

  • The dual specificity phosphatase transcriptome of the murine thymus.

    abstract::Properly regulated mitogen-activated protein (MAP) kinase activity is critical for normal thymocyte development. MAP kinases are activated by phosphorylation of tyrosine and threonine, and dual specificity phosphatases (DUSPs) can inactivate MAP kinases by dephosphorylating both tyrosine and threonine. However, a role...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Tanzola MB,Kersh GJ

    更新日期:2006-02-01 00:00:00

  • Fine molecular analysis of the antigenicity of the Androctonus australis hector scorpion neurotoxin II: a new antigenic epitope disclosed by the Pepscan method.

    abstract::A set of 58 overlapping rod-bound peptides was used to map the antigenic reactivity pattern of a 64-residue neurotoxin (AaH II) from the venom of the scorpion Androctonus australis hector. Five anti-toxin rabbit antisera were assayed serially for their capacity to bind to each peptide in the set. Six regions of antige...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Devaux C,Juin M,Mansuelle P,Granier C

    更新日期:1993-08-01 00:00:00

  • The secreted Candida albicans protein Pra1 disrupts host defense by broadly targeting and blocking complement C3 and C3 activation fragments.

    abstract::Candida albicans the most frequently isolated clinical fungal pathogen can cause local as well as systemic and life-threatening infections particularly in immune-compromised individuals. A better and more detailed understanding how C. albicans evades human immune attack is therefore needed for identifying fungal immun...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Luo S,Dasari P,Reiher N,Hartmann A,Jacksch S,Wende E,Barz D,Niemiec MJ,Jacobsen I,Beyersdorf N,Hünig T,Klos A,Skerka C,Zipfel PF

    更新日期:2018-01-01 00:00:00

  • A marker of human foetal endoderm defined by a monoclonal antibody involves Type 1 blood group chains.

    abstract::This report demonstrates that a marker of human embryonic endoderm and embryonal carcinoma cells recognized by a hybridoma antibody FC 10.2, involves Type 1 blood group chains with the sequence Gal beta 1 leads to 3G1cNAc beta 1 leads to 3Gal beta 1 leads to 4G1c. This conclusion has been reached from antigenic analys...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Gooi HC,Williams LK,Uemura K,Hounsell EF,McIlhinney RA,Feizi T

    更新日期:1983-06-01 00:00:00

  • Do Escherichia coli express Fc-receptors for immunoglobulin?

    abstract::A recent publication in Molecular Immunology by Leo and Goldman doi:10.1016/j.molimm.2009.02.024 has shown that the Escherichia coli immunoglobulin-binding (Eib) proteins are receptors for IgG and IgA. We would like to highlight important issues that were not addressed in their recent manuscript whilst providing possi...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Pleass RJ

    更新日期:2009-07-01 00:00:00

  • Maspin inhibits macrophage phagocytosis and enhances inflammatory cytokine production via activation of NF-κB signaling.

    abstract::Maspin (mammary serine protease inhibitor) is a non-inhibitory member of the serine protease inhibitor superfamily and a tumor suppressor in several cancers due to its ability to inhibit cell invasion, angiogenesis, and promote apoptosis. However, its immunomodulatory function remains largely unexplored. Thus, we expl...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Wang Y,Sun L,Song Z,Wang D,Bao Y,Li Y

    更新日期:2017-02-01 00:00:00

  • The complement system is involved in acute inflammation but not in the hemorrhage produced by a Bothrops atrox snake venom low molecular mass proteinase.

    abstract::Low molecular weight hemorrhagins were purified from crude Bothrops atrox snake venom by gel filtration followed by ionic strength chromatography. The protein fractions obtained, designated HI-1 to HI-8, contained proteins with molecular masses lower than 30 kDa. HI-5, the most representative among of these fractions,...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Rodrigues FG,Petretski JH,Kanashiro MM,Lemos L,da Silva WD,Kipnis TL

    更新日期:2004-03-01 00:00:00

  • Thermodynamic analysis of additivity between the heavy and light chains in affinity maturation of an antibody.

    abstract::In a recent article published in Molecular Immunology [Furukawa, K., Shimizu, T., Murakami, A., Kono, R., Nakagawa, M., Sagawa, T., Yamato, I., Azuma, T., 2007. Strategy for affinity maturation of an antibody with high evolvability to (4-hydroxy-3-nitrophenyl) acetyl hapten. Mol. Immunol. 44, 2436-2445], the authors m...

    journal_title:Molecular immunology

    pub_type: 评论,信件


    authors: Kang J,Warren AS

    更新日期:2008-01-01 00:00:00

  • Solid-phase antigen binding by purified immunoproteins from antigen-specific monoclonal T cell hybridomas.

    abstract::We have developed two distinct solid-phase immunoassays for the detection of antigen binding activity by products of antigen binding T cell hybridomas in the absence of MHC. Two suppressor T cell hybridomas studied (34s-18 and 34s-704) are specific for keyhole limpet hemocyanin, a protein antigen, and the other suppre...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Bristow CL,Marchalonis JJ

    更新日期:1989-07-01 00:00:00

  • Evidence for 65 electrophoretically distinct groups of light chains in BALB/c and NZB myelomas.

    abstract::Light chains of 426 randomly selected myelomas from BALB/c and NZB mice were characterized by isoelectric focusing (IF) and electrophoresis at pH 3. Approximately 50% of the light chains in each strain were found to have unique electrophoretic properties. The remaining 50% of light chains fell into groups of two or mo...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Gibson DM

    更新日期:1984-05-01 00:00:00

  • Monitoring the formation of soluble immune complexes composed of idiotype and anti-idiotype antibodies by electron microscopy.

    abstract::We have previously used immunoelectron microscopy (IEM) to generate a three-dimensional map of idiotypic (Id) and isotypic epitopes on the Fab arms of HGAC 39 (Roux et al., 1987, Proc. natn. Acad. Sci. U.S.A. 84, 4984-4988), a mouse IgG3 monoclonal antibody (Mab). In this report, we analyse the geometry of the various...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Roux KH,Greenspan NS

    更新日期:1994-06-01 00:00:00

  • Regulatory function of cell surface molecules CD2-, LFA- and beta 2-microglobulin in natural killer cell activity.

    abstract::The functional importance of various cell membrane bound molecules was studied and compared in the NK cytotoxicity and CTL activity. LFA-1 and CD2 participate in both killing functions, while CD3 and CD8/CD4 as well as MHC class I molecules are involved only in CTL activity. Nevertheless CD2- and beta 2-microglobulin ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Petrányi GG,Pócsik E,Kotlán B,Görög G,Benczur M

    更新日期:1986-11-01 00:00:00

  • HMGB1 induced inflammatory effect is blocked by CRISPLD2 via MiR155 in hepatic fibrogenesis.

    abstract::A number of studies have showed that High mobility group box-1 (HMGB1), which played key role in inflammation activation by triggering the toll like receptor 4 (TLR4) signaling axis in hepatic fibrogenesis, may share similar inflammation stimulating mechanism with LPS. Herein, we introduced a recently established anti...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Zhang H,Liu Z,Liu S

    更新日期:2016-01-01 00:00:00

  • Tumor PD-L1 co-stimulates primary human CD8(+) cytotoxic T cells modified to express a PD1:CD28 chimeric receptor.

    abstract::Tumors exploit immunoregulatory checkpoints that serve to attenuate T cell responses as a means of circumventing immunologic rejection. Programmed death ligand 1 (PD-L1) is a negative regulator of T cell function and is frequently expressed by solid tumors. By engaging programmed death 1 (PD-1) on activated T cells, P...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Prosser ME,Brown CE,Shami AF,Forman SJ,Jensen MC

    更新日期:2012-07-01 00:00:00

  • Comparative molecular modelling of the Fas-ligand and other members of the TNF family.

    abstract::A number of proteins with significant similarity to the tumour necrosis factor (TNF) have been identified over the last years. Upon interaction with their cognate receptor (members of the TNF-receptor family), all members of this protein family induce either cell death or proliferation/differentiation of the receptor-...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Peitsch MC,Tschopp J

    更新日期:1995-07-01 00:00:00

  • Localisation of the monocyte-binding region on human immunoglobulin G.

    abstract::Earlier studies, which provided indirect evidence for the involvement of the C gamma 2 domain of human immunoglobulin G (IgG) in human immunoglobulin G (IgG) in human monocyte binding, have been extended to further localise the site of interaction on human IgG. A number of IgGs from several different species and fragm...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Woof JM,Partridge LJ,Jefferis R,Burton DR

    更新日期:1986-03-01 00:00:00

  • Atomic resolution model of the antibody Fc interaction with the complement C1q component.

    abstract::The globular C1q heterotrimer is a subunit of the C1 complement factor. Binding of the C1q subunit to the constant (Fc) part of antibody molecules is a first step and key event of complement activation. Although three-dimensional structures of C1q and antibody Fc subunits have been determined experimentally no atomic ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Schneider S,Zacharias M

    更新日期:2012-05-01 00:00:00

  • The Mycobacterium tuberculosis glycoprotein Rv1016c protein inhibits dendritic cell maturation, and impairs Th1 /Th17 responses during mycobacteria infection.

    abstract::The myobacterial factors and the associated mechanism by which Mycobacterium tuberculosis (Mtb) evades the host immune surveillance system remain widely unexplored. Here, we found that overexpressing Rv1016c, a mannosylated protein of M. tuberculosis in BCG (rBCG-Rv1016c) led to increased virulence of the recombined B...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Su H,Peng B,Zhang Z,Liu Z,Zhang Z

    更新日期:2019-05-01 00:00:00

  • Engineered protein containing crotoxin epitopes induces neutralizing antibodies in immunized rabbits.

    abstract::Crotoxin (Ctx) is the main lethal component of Crotalus durissus terrificus venom. It is a neurotoxin, composed of two subunits associated by noncovalent interactions, the non-toxic acid subunit (CA), named Crotapotin, and the basic subunit (CB), with phospholipase A2 (PLA2) activity. Employing the SPOT synthesis tech...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Molina Molina DA,Guerra-Duarte C,Costal-Oliveira F,Almeida Rocha E,Rego Rodrigues C,Machado-de-Ávila RA,Soccol VT,Chávez-Olórtegui C

    更新日期:2020-03-01 00:00:00

  • Dynamics of CD3gammaepsilon and CD3deltaepsilon dimer expression during murine T cell development.

    abstract::The preTCR, gammadeltaTCR, and alphabetaTCR are the three isoforms of the T cell antigen receptor that are expressed during thymocyte development. Signaling by these isoforms is required at different stages of T cell development for lineage commitment, thymocyte maturation, and repertoire selection. All three isoforms...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Laird RM,Hayes SM

    更新日期:2009-12-01 00:00:00

  • Role of apoptotic signaling pathways in regulation of inflammatory responses to ricin in primary murine macrophages.

    abstract::Because of its lethal effects, ease of preparation, and ability to be delivered by aerosolization, ricin has been developed as a lethal weapon by various terrorist groups. When introduced into the pulmonary system of rodents, ricin causes pathological changes in the lung that are known to occur in acute respiratory di...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Korcheva V,Wong J,Lindauer M,Jacoby DB,Iordanov MS,Magun B

    更新日期:2007-04-01 00:00:00

  • AID- and Ung-dependent generation of staggered double-strand DNA breaks in immunoglobulin class switch DNA recombination: a post-cleavage role for AID.

    abstract::Class switch DNA recombination (CSR) substitutes an immunoglobulin (Ig) constant heavy chain (C(H)) region with a different C(H) region, thereby endowing an antibody with different biological effector functions. CSR requires activation-induced cytidine deaminase (AID) and occurrence of double-strand DNA breaks (DSBs) ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Zan H,Casali P

    更新日期:2008-11-01 00:00:00

  • Mass spectrometry-based relative quantification of human neutrophil peptides 1, 2, and 3 from biological samples.

    abstract::Human neutrophil peptides (HNPs) are cysteine-rich antimicrobial peptides stored in neutrophils. The similar structure of HNPs -1, -2, and -3 renders them impossible to study individually in biological samples. For the first time, we describe a method of individually identifying the HNPs -1-3 from exudative neutrophil...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Thompson L,Turko I,Murad F

    更新日期:2006-03-01 00:00:00