Engineered protein containing crotoxin epitopes induces neutralizing antibodies in immunized rabbits.

abstract：:T regulatory (Treg)/T-helper (Th) 17 imbalance has been shown to integrate with epigenetics to result in the development of autoimmune diseases. We aim to investigate the influence of disease staging on Treg/Th17 cells and whether the aberrant DNA methylation is implicated in the development of rheumatoid arthritis (R...

journal_title：Molecular immunology

authors： Huang Y,Wang H,Ba X,Chen Z,Wang Y,Qin K,Huang Y,Shen P,Tu S

更新日期：2020-11-01 00:00:00

abstract：:Affinity-purified major histocompatibility complex (MHC) class II molecules are known to bind antigenic peptides in vitro. The percentage of MHC class II molecules occupied with such peptides is usually very low and varies significantly depending upon the sequence and size of a given antigenic peptide. The present stu...

journal_title：Molecular immunology

authors： Nag B,Mukku PV,Arimilli S,Phan D,Deshpande SV,Winkelhake JL

更新日期：1994-10-01 00:00:00

abstract：:Factor I (C3b/C4b inactivator) is a regulatory protein of the classical and alternative complement pathways. In this paper, we report the sequence of Xenopus factor I cDNA and the deduced protein structure. The basic structure of human preprofactor I, NH2-heavy chain-cleavage peptide-light chain-COOH, is conserved in ...

journal_title：Molecular immunology

authors： Kunnath-Muglia LM,Chang GH,Sim RB,Day AJ,Ezekowitz RA

更新日期：1993-10-01 00:00:00

abstract：:The Pacific oyster (Crassostrea gigas) is farmed globally. Ostreid herpesvirus (OsHV-1) causes severe mortalities of farmed C. gigas. Management of OsHV-1 has proven difficult. Oysters treated with poly(I:C) exhibit enhanced protection (EP) against OsHV-1. This chemical treatment is highly effective, but it is not fea...

journal_title：Molecular immunology

authors： Green TJ,Helbig K,Speck P,Raftos DA

更新日期：2016-10-01 00:00:00

abstract：:Covalent Superose microspheres-bound C3b was used as a model system to simplify the analysis of antigen-bound C3b modifications during antigen processing. The model was set up using purified C3 and Superose-bound trypsin. C3b was covalently bound to Superose through an ester link, as indicated by lability to hydroxyla...

journal_title：Molecular immunology

authors： Rey-Millet CA,Chesne S,Colomb MG

更新日期：1993-07-01 00:00:00

abstract：:For decades immunological research has relied, with variable success, on mouse models to investigate diseases and possible therapeutic interventions. With the approval of the first therapeutic antibody targeting complement, called eculizumab, as therapy in paroxysmal nocturnal hemoglobinuria (PNH) and more recently at...

journal_title：Molecular immunology

authors： Pouw RB,Vredevoogd DW,Kuijpers TW,Wouters D

更新日期：2015-09-01 00:00:00

abstract：:In a recent article published in Molecular Immunology [Furukawa, K., Shimizu, T., Murakami, A., Kono, R., Nakagawa, M., Sagawa, T., Yamato, I., Azuma, T., 2007. Strategy for affinity maturation of an antibody with high evolvability to (4-hydroxy-3-nitrophenyl) acetyl hapten. Mol. Immunol. 44, 2436-2445], the authors m...

journal_title：Molecular immunology

authors： Kang J,Warren AS

更新日期：2008-01-01 00:00:00

abstract：:Lipopolysaccharide (LPS) present on the outer membrane of Gram-negative bacteria is one of the most important pathogen-associated molecular patterns and a potent elicitor in innate immunity. In human, TLR4 (Toll-like receptor 4) and MD-2 (myeloid differiation-2) form a receptor complex to transduce the LPS signal into...

journal_title：Molecular immunology

authors： Ao JQ,Ling E,Rao XJ,Yu XQ

更新日期：2008-05-01 00:00:00

abstract：:A new sulfhydryl redox model of antibody-dependent endocytosis is proposed. This model synthesizes many previous studies employing a variety of experimental approaches. Predictions of this model can be tested experimentally. ...

journal_title：Molecular immunology

authors： Petty HR

更新日期：1985-08-01 00:00:00

abstract：:ASF1a (anti-silencing function 1a), an evolutionarily conserved protein and a histone chaperone, is required for a variety of chromatin-mediated cellular processes. However, the function of ASF1a in innate immune response remains unclear. Here, we find that ASF1a is induced in Vesicular Stomatitis Virus (VSV)-infected...

journal_title：Molecular immunology

authors： Liu Z,Yang L,Sun Y,Xie X,Huang J

更新日期：2016-10-01 00:00:00

abstract：:The mechanisms of each subset of immune cells contributing to the pathogenesis of viral hepatitis remain incompletely understood. In this study, we examined the role of liver CD4(-) CD8(-) (double negative, DN) T cells during murine hepatitis virus strain 3 (MHV-3)-induced hepatitis in C3H/HeJ mice. We demonstrate tha...

journal_title：Molecular immunology

authors： Lu Y,Wang X,Yan W,Wang H,Wang M,Wu D,Zhu L,Luo X,Ning Q

更新日期：2012-10-01 00:00:00

abstract：:Evidence suggests that immune dysregulation is associated with autism spectrum disorder (ASD). T cell immunoglobulin and mucin domain-3 (TIM-3) has a critical role in several inflammatory disorders; however, the role of TIM-3 signaling has not been demonstrated in ASD. In the present study, we assessed the role of TIM...

journal_title：Molecular immunology

authors： Ahmad SF,Ansari MA,Nadeem A,Bakheet SA,Al-Ayadhi LY,Alotaibi MR,Alhoshani AR,Alshammari MA,Attia SM

更新日期：2019-02-01 00:00:00

abstract：:Although the beneficial effects of probiotics on wide variety of diseases have been shown, little is known about how probiotics modulate the immune system. In this study we elucidated the underlying mechanisms how Lactobacillus casei (L. casei) protects against rheumatoid arthritis (RA) progression by investigating th...

journal_title：Molecular immunology

authors： So JS,Kwon HK,Lee CG,Yi HJ,Park JA,Lim SY,Hwang KC,Jeon YH,Im SH

更新日期：2008-05-01 00:00:00

abstract：:A number of fragments derived from acid-treated rabbit IgG by digestion with plasmin have been separated by high-resolution gel filtration. Fragments isolated included a dimer and monomer Facb, named F(acb)2 and Facb, respectively and a heterodimer composed of Facb and Fab subunits, named F(acb)(ab). A C gamma 2 fragm...

journal_title：Molecular immunology

authors： Utsumi S,Okada M,Udaka K,Amano T

更新日期：1985-07-01 00:00:00

abstract：:Major histocompatibility complex (MHC) class I and II proteins have been in the focus of interest for immunologists, biochemists, cell biologists, and structural biologists for decades. With dozens of entries in the Protein Data Bank, their crystal structures are now sufficiently well understood, while their dynamic p...

journal_title：Molecular immunology

authors： Yaneva R,Schneeweiss C,Zacharias M,Springer S

更新日期：2010-01-01 00:00:00

abstract：:This paper describes additional structural analyses of the p24 cell surface molecule recognized by monoclonal antibody BA-2. Since BA-2 is broadly reactive with a variety of normal and malignant lymphohematopoietic and nonlymphohematopoietic cells, we examined the structure of p24 expressed on different cell types. Tr...

journal_title：Molecular immunology

authors： LeBien TW,Pirruccello SJ,McCormack RT,Bradley JG

更新日期：1985-10-01 00:00:00

abstract：:A study aiming at cloning and characterizing natural antibodies to human immunodeficiency virus type 1 (HIV-1) targets is described. In particular, we report the molecular cloning of a Fab molecule binding the HIV-1/Tat protein from a seronegative patient. The Fab was characterized for its binding specificity and inve...

journal_title：Molecular immunology

authors： Mancini N,Perotti M,Carletti S,Canducci F,Sampaolo M,Clementi M,Burioni R

更新日期：2006-03-01 00:00:00

abstract：:Dendritic cells (DC) are comprised of several subsets with distinct functions, differing in their capacity to respond to pathogen products. To gain novel insights into their pathogen specificity, we compared the protein composition of the plasma membrane of CD8+ and CD8- DC, directly isolated from mouse spleens. Diffe...

journal_title：Molecular immunology

authors： Segura E,Kapp E,Gupta N,Wong J,Lim J,Ji H,Heath WR,Simpson R,Villadangos JA

更新日期：2010-05-01 00:00:00

abstract：:T-lymphocyte proliferative responses were determined with peripheral blood lymphocytes (PBL), peritoneal exudate lymphocytes (PEL) and lymph node lymphocytes (LNL) of guinea pigs immunized with horse cytochrome c (HCytc). The proliferative response of PBL activated by peptide 81-104 from animals immunized with HCytc w...

journal_title：Molecular immunology

authors： Wang KM,Reichlin M

更新日期：1988-08-01 00:00:00

abstract：:Idiotype (Id) and autoanti-thyroglobulin were induced in different strains of mice by priming with anti-Id to monoclonal anti-thyroglobulins (D8 and G4) and challenged with a subimmunogenic dose of thyroglobulin (Tg). Both D8.Id and G4.Id were induced in CBA mice by priming with the appropriate anti-Id, but only primi...

journal_title：Molecular immunology

authors： Male D,Pryce G,Roitt I

更新日期：1985-03-01 00:00:00

abstract：:The SARS-CoV-2 virus responsible for coronavirus 2019 (COVID-19) poses a significant challenge to healthcare systems worldwide. According to the World Health Organization (WHO), the outbreak of COVID-19 has been a pandemic that infected more than 25.32 million people and caused more than 848.25 thousand deaths worldwi...

journal_title：Molecular immunology

authors： Liu D,Wu F,Cen Y,Ye L,Shi X,Huang Y,Fang S,Ma L

更新日期：2021-01-09 00:00:00

abstract：:We designed the peptide (C3) mimetic carboxyl-terminus (Cterminus) of capsid protein of porcine circovirus type 2b (PCV2b-1A/1B) inducing humoral immunity and generating hybridomas. The positive reactivity of the mAbs to PCV2 capsid protein was demonstrated by Western blot assay. Those mAbs also showed positive signal...

journal_title：Molecular immunology

authors： Hung LC,Cheng IC

更新日期：2017-05-01 00:00:00

abstract：:T-cell-replacing factor (TRF)/IL-5 is a T-cell-derived glycoprotein which has pleiotropic activity on lymphoid and myeloid cells. IL-5 polypeptide translated into Xenopus oocytes are heterogeneous in molecular size (40,000 to 60,000 under nonreducing conditions) and yields a monomeric form (Mr of 25,000 to 30,000) und...

journal_title：Molecular immunology

authors： Takahashi T,Yamaguchi N,Mita S,Yamaguchi Y,Suda T,Tominaga A,Kikuchi Y,Miura Y,Takatsu K

更新日期：1990-09-01 00:00:00

abstract：:The major product of digestion of bovine IgG2a(A1) with immobilized pepsin is F(ab')2 while similar treatment of IgG2a(A2) yields Fab, Fc and other products. It is postulated that structural differences in the hinge region, including the absence of the most N-terminal disulfide bridge in IgG2a(A2) and a displacement o...

journal_title：Molecular immunology

authors： Heyermann H,Butler JE

更新日期：1987-12-01 00:00:00

abstract：:The complement system is a vital component of the immune-priveliged human eye that is always active at a low-grade level, preventing harmful intraocular injuries caused by accumulation of turnover products and controlling pathogens to preserve eye homeostasis and vision. The complement system is a double-edged sword t...

journal_title：Molecular immunology

authors： Mohlin C,Sandholm K,Ekdahl KN,Nilsson B

更新日期：2017-09-01 00:00:00

abstract：:Interplay between complement factors, regulatory proteins, anaphylatoxins and cytokines could be involved in tendon healing and scar formation. The expression and regulation of complement factors by cytokines or anaphylatoxins are completely unclear in tendon. Hence, the gene expression of the anaphylatoxin receptors ...

journal_title：Molecular immunology

authors： Busch C,Girke G,Kohl B,Stoll C,Lemke M,Krasnici S,Ertel W,Silawal S,John T,Schulze-Tanzil G

更新日期：2013-04-01 00:00:00

abstract：:The primary structure of a human mu heavy chain (DAG) protein is described. The native protein is a circular decamer with a molecular weight (Mr) of 500 kDa, each decamer being constituted of the constant domains C mu 2, C mu 3 and C mu4 and interlinked by 15 disulfide bridges. At its NH2-terminal each monomeric chain...

journal_title：Molecular immunology

authors： Mihaesco C,Ferrara P,Guillemot JC,Congy N,Gendron MC,Roy JP,Sizaret PY,Mihaesco E

更新日期：1990-08-01 00:00:00

abstract：:Four cell lines producing monoclonal antibodies were obtained by fusion of NS1 myeloma cells with splenocytes of BALB/C mice immunized with only 1 microgram of each staphylococcal enterotoxin A, B, C1 and D by a modified technique of intrasplenic boosting. This procedure was considerably more efficient than the more c...

journal_title：Molecular immunology

authors： Lapeyre C,Kaveri SV,Janin F,Strosberg AD

更新日期：1987-12-01 00:00:00

abstract：:The alternative pathway of complement is receiving increasing attention as a therapeutic target because of recent findings in several animal models that support its essential role in tissue injury and disease pathogenesis. Although the contribution of alternative pathway activation to serum complement activation in vi...

journal_title：Molecular immunology

authors： Holers VM,Thurman JM

更新日期：2004-06-01 00:00:00

abstract：BACKGROUND AND OBJECTIVES:The complement system plays an important role in the development of acute coronary syndrome (ACS). Complement C1q is an important initial component of the classical complement pathway and closely related to many chronic inflammatory diseases, including atherosclerosis (AS). We aimed to determi...

journal_title：Molecular immunology

authors： Ni XN,Yan SB,Zhang K,Sai WW,Zhang QY,Ti Y,Wang ZH,Zhang W,Zheng CY,Zhong M

更新日期：2020-04-01 00:00:00