Abstract:
:We previously demonstrated that some fish have unique response in the form of reactive oxygen species (ROS) production. In the present study, we cloned and sequenced the full-length cDNAs for carp (Cyprinus carpio) phagocyte NADPH oxidase components: gp91phox, p22phox, p47phox, p67phox and p40phox. These amino acid sequences were compared with other teleost and mammalian homologues, to elucidate the features of ROS production of fish neutrophils. The phylogeny analysis clearly demonstrates that the radiation of phagocyte oxidase components took place in the common ancestor of teleosts and mammals. Thereafter, the overall structure and expression pattern of phagocyte oxidase have been highly conserved in two different strains. However the amino acid identity of p67phox and p47phox was relatively lower than the amino acid identities of other components. Moreover, a synteny analysis supports the hypothesis that there was strong selective pressure in the p67phox and p47phox genes. Thus, it is likely that the higher divergence of p67phox and p47phox are responsible for the difference of ROS responses between different species of teleosts.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Mayumi M,Takeda Y,Hoshiko M,Serada K,Murata M,Moritomo T,Takizawa F,Kobayashi I,Araki K,Nakanishi T,Sumimoto Hdoi
10.1016/j.molimm.2007.09.028subject
Has Abstractpub_date
2008-03-01 00:00:00pages
1720-31issue
6eissn
0161-5890issn
1872-9142pii
S0161-5890(07)00780-8journal_volume
45pub_type
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