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DNA immunization via intramuscular and intradermal routes using a gene gun provides different magnitudes and durations on immune response.

Abstract：

:We investigated the antibody (Ab) and cytotoxic T lymphocyte (CTL) responses to gene gun (GG)-mediated DNA immunization via the intramuscular (i.m.) and intradermal (i.d.) routes. BALB/c mice were immunized five times at weekly intervals with plasmid DNA encoding enhanced green fluorescent protein (EGFP). EGFP production was rapidly detected in the target tissues after injection via either delivery route. There were significant differences in the magnitude and duration of the Ab and CTL responses according to the route employed. Intradermal injection elicited higher Ab and CTL responses to EGFP than i.m. injection 1 week after the last immunization. However, both immune responses were reduced rapidly 5 weeks after the last immunization via i.d. injection. In contrast, in mice injected via the i.m. routes, Ab and CTL responses 5 weeks after the last immunization remained at levels similar to those detected after 1 week. All mice generated a predominantly IgG1 Ab response via either route. These findings suggest that a combination of these two routes of DNA immunization would provide optimal conditions for induction of a broad immune response, and this information is expected to be very important for future applications of DNA vaccination.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Ito K,Ito K,Shinohara N,Kato S

doi

10.1016/s0161-5890(03)00024-5

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

847-54

issue

14

eissn

0161-5890

issn

1872-9142

pii

S0161589003000245

journal_volume

39

pub_type

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