当前位置： SCI文献检索 > MOLECULAR IMMUNOLOGY期刊下所有文献 > Involvement of Notch signaling pathway in regulating IL-12 expression via c-Rel in activated macrophages.

Involvement of Notch signaling pathway in regulating IL-12 expression via c-Rel in activated macrophages.

Abstract：

:Macrophages play an important role both in innate and adaptive immune responses. Treatment with interferon (IFN) γ together with lipopolysaccharide (LPS) activates pro-inflammatory macrophages which secrete various pro-inflammatory cytokines including IL-12. IL-12 promotes a Th1 type immune response by directly controlling the differentiation of CD4(+) T helper 1 cells. Activation of Notch signaling pathway was reported in activated macrophages but the involvement of this signaling pathway in IL-12 expression has not been documented. In this study, we investigated the role of Notch signaling in regulating expression of the IL-12/IL-23 subunit, IL-12p40. Using a gamma-secretase inhibitor (GSI) to inhibit Notch signaling, we observed a profound decrease in il12p40 mRNA levels and IL-12p70 secretion upon IFNγ/LPS stimulation. On the other hand, overexpression of activated form of Notch1 in activated RAW264.7 macrophage-like cell lines significantly increased the level of il12p40 mRNA. GSI treatment did not affect the expression of irf5, a master regulator of il12p40 transcription in macrophages. Detailed analysis of the signaling cascades that were affected by this inhibition showed that c-Rel nuclear translocation was inhibited and Erk1/2 activation was compromised by GSI treatment. Addition of exogenous tumor necrosis factor (TNF) α only partially rescued the expression of il12p40 in the presence of GSI. Unexpectedly, inhibition of Notch signaling using a dominant negative (DN) Mastermind-like (MAML) transcription co-activator, did not affect c-Rel nuclear localization upon activation or il12p40 mRNA levels, suggesting that the transcriptional activity of Notch signaling is dispensable for the activation of c-Rel. These results strongly suggest that Notch signaling in activated macrophages is involved in regulating the expression of il12p40 directly via c-Rel and indirectly via TNFα production.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Boonyatecha N,Sangphech N,Wongchana W,Kueanjinda P,Palaga T

doi

10.1016/j.molimm.2012.03.017

subject

Has Abstract

pub_date

2012-07-01 00:00:00

pages

255-62

issue

3-4

eissn

0161-5890

issn

1872-9142

pii

S0161-5890(12)00193-9

journal_volume

51

pub_type

杂志文章

相关文献

MOLECULAR IMMUNOLOGY文献大全
  • SOCS3-deficient lung epithelial cells uptaking neutrophil-derived SOCS3 worsens lung influenza infection.

    abstract：:Suppressor of cytokine signaling 3 (SOCS3) is a negative regulator of TBK1 and interferon pathway and the expression of SOCS3 is closely correlated with symptoms of influenza patients. However, whether deletion of Socs3 in the lung epithelial cells would affect influenza lung replication and inflammation in vivo is un...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2020.06.022

    authors： Li L,Wu H,Li Q,Chen J,Xu K,Xu J,Su X

    更新日期：2020-09-01 00:00:00

  • CD4 regulation in human lymphoid non-T-cells: a role for the silencer element.

    abstract：:In humans, the CD4 molecule is expressed on a subset of T-cells and at various levels on myeloid and lymphoid cells. The mechanisms regulating human CD4 gene expression are yet poorly understood. We speculated that the CD4 silencer, which operates in CD8+ T-cells to repress CD4 expression, could be responsible for CD4...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2006.03.002

    authors： Mouly E,Dorival C,Pflumio F,Baillou C,Coulombel L,Levy Y,Lemoine FM,Klatzmann D,Marodon G

    更新日期：2007-01-01 00:00:00

  • Potentiation of factor H by heparin: a rate-limiting mechanism for inhibition of the alternative complement pathway.

    abstract：:The mechanism by which heparin inhibits the alternative complement pathway (ACP) by a fluid-phase activator, CoVF, has been studied. Results presented here indicate that heparin's major (rate-limiting) effect on the fluid-phase activation of the ACP was to potentiate Factor H activity. Such an effect results in a very...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(83)90139-6

    authors： Boackle RJ,Caughman GB,Vesely J,Medgyesi G,Fudenberg HH

    更新日期：1983-11-01 00:00:00

  • Bone morphogenetic protein 6-induced interleukin-1β expression in macrophages requires PU.1/Smad1 interaction.

    abstract：:Interleukin 1β (IL-1β) is a pro-inflammatory cytokine secreted by activated macrophages and monocytes. Previously, we have reported that bone morphogenetic protein-6 (BMP-6) induces inducible nitric oxide synthase (iNOS) expression via IL-1β in macrophages. In the present study, we demonstrate that BMP-6 increases IL-...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2011.04.019

    authors： Lee GT,Jung YS,Lee JH,Kim WJ,Kim IY

    更新日期：2011-07-01 00:00:00

  • Inhibition of the transcription factor c-Jun by the MAPK family, and not the NF-κB pathway, suggests that peanut extract has anti-inflammatory properties.

    abstract：BACKGROUND:Tumor necrosis factor-α (TNF-α) is involved in inflammatory responses in atherosclerosis. We propose an in vitro cellular assay to evaluate the anti-inflammatory mechanisms of potential modifiers such as food extracts. In the current model we assessed an anti-inflammatory effect of polyphenol-rich peanut ext...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2012.05.007

    authors： Catalán Ú,Fernández-Castillejo S,Anglès N,Morelló JR,Yebras M,Solà R

    更新日期：2012-10-01 00:00:00

  • Immunochemical and related interactions with dextrans reviewed in terms of improved structural information.

    abstract：:Structurally diverse dextrans from Leuconostoc mesenteroides and related bacteria have been used extensively in fundamental immunochemical studies such as induction and characterization of anti-dextran antibodies, as well as in studies of their interaction with pneumococcal antisera, normal bovine serum, concanavalin ...

    journal_title：Molecular immunology

    pub_type： 杂志文章,评审

    doi：10.1016/0161-5890(86)90131-8

    authors： Jeanes A

    更新日期：1986-09-01 00:00:00

  • Homology analysis and cross-immunogenicity of OmpA from pathogenic Yersinia enterocolitica, Yersinia pseudotuberculosis and Yersinia pestis.

    abstract：:The outer membrane protein A (OmpA) is one of the intra-species conserved proteins with immunogenicity widely found in the family of Enterobacteriaceae. Here we first confirmed OmpA is conserved in the three pathogenic Yersinia: Yersinia pestis, Yersinia pseudotuberculosis and pathogenic Yersinia enterocolitica, with ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2015.09.016

    authors： Chen Y,Duan R,Li X,Li K,Liang J,Liu C,Qiu H,Xiao Y,Jing H,Wang X

    更新日期：2015-12-01 00:00:00

  • Molecular comparison of mouse Thy-1 and its human homologue.

    abstract：:Physicochemical techniques were used to verify previous immunochemical studies showing homology of the human Thy-1 (formerly p25) antigen and the murine Thy-1 or theta antigen. Peptide maps and amino acid compositions showed close similarity between these two proteins; however, they were not identical. These data conf...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(82)90216-4

    authors： Bonewald L,Wang AC,Terry E,Zwerner R,Balch CM,Ades EW

    更新日期：1982-03-01 00:00:00

  • Fine specificity of a monoclonal anti-testicular cell antibody for glycolipids with terminal N-acetyl-D-glucosamine structure.

    abstract：:The specificity of a mouse anti-testicular cell monoclonal antibody, J1, was investigated. Previous studies suggested that N-acetyl-D-glucosamine (GlcNAc) was a constituent of the determinant recognized by J1. When the antibody was tested against a variety of purified glycolipids containing this saccharide in terminal...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(84)90142-1

    authors： Symington FW,Fenderson BA,Hakomori S

    更新日期：1984-10-01 00:00:00

  • Cloning and molecular characterization of a human recombinant IgG Fab binding to the Tat protein of human immunodeficiency virus type 1 (HIV-1) derived from the repertoire of a seronegative patient.

    abstract：:A study aiming at cloning and characterizing natural antibodies to human immunodeficiency virus type 1 (HIV-1) targets is described. In particular, we report the molecular cloning of a Fab molecule binding the HIV-1/Tat protein from a seronegative patient. The Fab was characterized for its binding specificity and inve...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2005.08.003

    authors： Mancini N,Perotti M,Carletti S,Canducci F,Sampaolo M,Clementi M,Burioni R

    更新日期：2006-03-01 00:00:00

  • IL-2 regulates the expression of the NK-TR gene via an alternate RNA splicing mechanism.

    abstract：:We have recently isolated and characterized human and mouse genes of a putative natural killer (NK) cell tumour-recognition protein (NK-TR) that is specifically expressed in NK cells. This gene codes for a 150 kD protein with a cyclophilin-related amino terminus followed by several positively charged domains. We repor...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(93)90047-f

    authors： Rinfret A,Anderson SK

    更新日期：1993-10-01 00:00:00

  • A hemocyte-specific cathepsin L-like cysteine protease is involved in response to 20-hydroxyecdysone and microbial pathogens stimulation in silkworm, Bombyx mori.

    abstract：:Cathepsin L protease belongs to the papain-like cysteine proteases family, plays indispensable roles in animals' pathological and physiological processes. However, little is known about Cathepsin L in silkworm, Bombyx mori. Herein, a novel Cathepsin L-like (Cat L-like) was cloned and identified from silkworm by the ra...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2020.12.013

    authors： Pan G,Zhang K,Li C,Hu X,Kausar S,Gu H,Yang L,Cui H

    更新日期：2020-12-26 00:00:00

  • A unique Vgamma5-T-cell population in the murine mammary gland.

    abstract：:Mammary epithelia and the epidermis share a common embryologic origin. Like resident epidermal T cells (ETC), a significant number of murine mammary gland T cells (MTC) express Vgamma5-T-cell receptors (TCR). MTC were assessed to see if they express the same invariant Vgamma5/Vdelta1-TCRs as those of ETC. MTC-derived ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2005.05.010

    authors： Reardon C,Born WK,O'Brien RL

    更新日期：2006-03-01 00:00:00

  • Normal TCRbeta transcription and recombination in the absence of the Jbeta2-Cbeta2 intronic cis element.

    abstract：:The developmental regulation of antigen receptor gene transcription and recombination are mediated by cis regulatory elements. At the T cell receptor beta chain locus (TCRbeta), two DNase I hypersensitive sites within the Jbeta2-Cbeta2 intron contained binding sites for NF-kappaB and additional nuclear factors and wer...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/s0161-5890(01)00031-1

    authors： Whitehurst CE,Hu H,Ryu CJ,Rajendran P,Schmidt T,Chen J

    更新日期：2001-01-01 00:00:00

  • Allogeneic and syngeneic class I MHC complexes drive the association of CD8 and TCR on 2C T cells.

    abstract：:In most cases, cytotoxic T cell activation is dependent on the interaction of the T cell receptor (TCR) and CD8 with MHC class I molecules. In the CD8(+) T cell system based on the mouse cytotoxic T cell clone 2C, recognition of the allogeneic MHC L(d) exhibits a less significant role for CD8 than recognition of the s...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/s0161-5890(02)00259-6

    authors： Lee PU,Kranz DM

    更新日期：2003-01-01 00:00:00

  • The interaction between C/EBPβ and TFAM promotes acute kidney injury via regulating NLRP3 inflammasome-mediated pyroptosis.

    abstract：:Sepsis-induced inflammatory damage is a crucial cause of acute kidney injury (AKI), and AKI is an ecumenical fearful complication in approximately half of patients with sepsis. CCAAT/enhancer-binding protein β (C/EBPβ) plays roles in regulating acute phase responses and inflammation. However, the role and mechanism of...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2020.08.023

    authors： Dai XG,Li Q,Li T,Huang WB,Zeng ZH,Yang Y,Duan ZP,Wang YJ,Ai YH

    更新日期：2020-11-01 00:00:00

  • Active phagocytosis of Mycobacterium tuberculosis (H37Ra) by T lymphocytes (Jurkat cells).

    abstract：:This study aimed to co-culture Jurkat T lymphocytes with inactivated Mycobacterium tuberculosis (Mtb H37Ra), explore whether T lymphocytes could phagocytose H37Ra cells, and determine the underlying mechanism. Jurkat T lymphocytes were co-cultured with H37Ra cells, and confocal laser scanning microscopy, electron micr...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2015.04.018

    authors： Zhang M,Zhu Q,Shi M,Liu Y,Ma L,Yang Y,Feng D,Dai W,Zhang L,Kang T,Chen P,He Y,Liu T,Zhao Q,Wang W,Zhi J,Feng G,Zhao G

    更新日期：2015-08-01 00:00:00

  • MLKL deficiency inhibits DSS-induced colitis independent of intestinal microbiota.

    abstract：:The maintenance of intestinal tissue homeostasis is vital for the resistance against inflammatory bowel diseases (IBDs). Necroptosis is identified as an alternative mode of regulated cell death, which plays a pivotal role in tissue homeostasis. Thus, the roles of RIP3-mediated necroptosis in intestinal inflammation ha...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2019.01.018

    authors： Zhang J,Qin D,Yang YJ,Hu GQ,Qin XX,Du CT,Chen W

    更新日期：2019-03-01 00:00:00

  • Identification of Porphyromonas gingivalis lipopolysaccharide-binding proteins in human saliva.

    abstract：:Porphyromonas gingivalis causes periodontal diseases and its lipopolysaccharide (LPS) is considered as a major virulence factor responsible for pathogenesis. Since initial recognition of P. gingivalis LPS (Pg.LPS) in the oral cavity might be crucial for the host response, we identified Pg.LPS-binding proteins (Pg.LPS-...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2011.06.434

    authors： Choi S,Baik JE,Jeon JH,Cho K,Seo DG,Kum KY,Yun CH,Han SH

    更新日期：2011-09-01 00:00:00

  • Mapping the upstream boundary of somatic mutations in rearranged immunoglobulin transgenes and endogenous genes.

    abstract：:Mammalian B-cell specific somatic hypermutation contributes to affinity maturation of the antibody response. This mutator activity is highly focused on rearranged immunoglobulin variable regions, but the underlying mechanism remains to be elucidated. In an effort to gain insights into the mechanism of somatic hypermut...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(94)90081-7

    authors： Rogerson BJ

    更新日期：1994-02-01 00:00:00

  • Hydrophobic strip-of-helix algorithm for selection of T cell-presented peptides.

    abstract：:In extension of the hypothesis that an amphipathic alpha helix of Ii (Phe146-Val164) bound to the foreign antigen-presenting site (desetope) of class II MHC molecules through hydrophobic amino acid residues (Phe146, Leu150, Leu153, Met157, Ile160, Val164) which were present in an axial strip along one side of the Ii h...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(87)90068-x

    authors： Stille CJ,Thomas LJ,Reyes VE,Humphreys RE

    更新日期：1987-10-01 00:00:00

  • Structural analyses define an additional H-2D region class I antigen that is expressed by a variant mouse strain.

    abstract：:The genetic complexity of the H-2D region includes haplotype disparities in apparent gene and product number. To probe the genetic basis of this complexity, the products of two independently derived mouse strains (STU and B10.SAA48) that express Dw3 antigens were compared. Serologic, fluorometric and peptide map compa...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(86)90007-6

    authors： Lee DR,Lillehoj EP,Myers NB,Coligan JE,Hansen TH

    更新日期：1986-10-01 00:00:00

  • Decipher manifestations and Treg /Th17 imbalance in multi-staging rheumatoid arthritis and correlation with TSDR/RORC methylation.

    abstract：:T regulatory (Treg)/T-helper (Th) 17 imbalance has been shown to integrate with epigenetics to result in the development of autoimmune diseases. We aim to investigate the influence of disease staging on Treg/Th17 cells and whether the aberrant DNA methylation is implicated in the development of rheumatoid arthritis (R...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2020.08.002

    authors： Huang Y,Wang H,Ba X,Chen Z,Wang Y,Qin K,Huang Y,Shen P,Tu S

    更新日期：2020-11-01 00:00:00

  • The activation of the TLR2/p38 pathway by sodium butyrate in bovine mammary epithelial cells is involved in the reduction of Staphylococcus aureus internalization.

    abstract：:Staphylococcus aureus is an etiological agent of human and animal diseases, and it is able to internalize into non-professional phagocytic cells (i.e. bovine mammary epithelial cells, bMECs), which is an event that is related to chronic and recurrent infections. bMECs contribute to host innate immune responses (IIR) t...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2015.09.025

    authors： Alva-Murillo N,Medina-Estrada I,Báez-Magaña M,Ochoa-Zarzosa A,López-Meza JE

    更新日期：2015-12-01 00:00:00

  • Murine anti-TNP antibodies positive for either lambda 1 or lambda 2 light chains express common idiotypic determinants.

    abstract：:Although the lambda-bearing antibodies represent only 5% of the total mouse serum immunoglobulins, some antigens such as B1355 dextran (alpha (1-3)Dex), the 4-hydroxy-3-nitrophenyl acetyl (NP) and 2,4-dinitro or 2,4,6-trinitrophenyl (DNP/TNP) antigens can induce lambda-positive immune responses. In contrast to the lam...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(88)90061-2

    authors： Sanchez P,Juy D,Cazenave PA

    更新日期：1988-02-01 00:00:00

  • Involvement of CTLA-4 in T-cell anergy induced by staphylococcal enterotoxin A in vitro.

    abstract：:Superantigens, like staphylococcal enterotoxin A (SEA), induce a strong proliferative response followed by clonal deletion of a substantial portion of defined V(beta) T-cells. The remaining cells display in vitro anergy. Anergy is a major mechanism to ensure antigen-specific tolerance in T-lymphocyte in the adult. Co-...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2004.03.001

    authors： Xu GL,Zhu XH,Guo B,Wu YZ

    更新日期：2004-05-01 00:00:00

  • Amino acid sequence of the variable region of heavy chain in immunoglobulin (Mot) having unusual papain cleavage sites.

    abstract：:An IgGl(lambda) Mot myeloma protein showed a unique susceptibility toward papain digestion. The Fab fragment of Mot was more digestible with papain than the Fc fragment. This phenomenon was found to occur by unusual cleavage of the Fd fragment with papain. Determination of the complete primary structure of the V regio...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(86)90039-8

    authors： Kojima M,Koide T,Odani S,Ono T

    更新日期：1986-02-01 00:00:00

  • Biased dA/dT somatic hypermutation as regulated by the heavy chain intronic iEmu enhancer and 3'Ealpha enhancers in human lymphoblastoid B cells.

    abstract：:Somatic hypermutation (SHM) in immunoglobulin gene (Ig) variable (V) regions is critical for the maturation of the antibody response. It is dependent on the expression of activation-induced cytidine deaminase (AID) and translesion DNA polymerases in germinal center B cells as well as Ig V transcription, as regulated b...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2005.10.018

    authors： Komori A,Xu Z,Wu X,Zan H,Casali P

    更新日期：2006-04-01 00:00:00

  • HIV-1 gp41 binds to several proteins on the human B-cell line, Raji.

    abstract：:Based on our findings that HIV-1 gp41 independently of CD4, can bind to the helper T-cell line H9, we characterized putative binding of HIV-1 gp41 to B-cell lines, Raji, Bjab and Ramos. Using fluorescence-activated cell sorter (FACS) we examined the binding of soluble gp41 (sgp41; Env amino acid 539-684) to these B-ce...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(93)90134-w

    authors： Chen YH,Böck G,Vornhagen R,Steindl F,Katinger H,Dierich MP

    更新日期：1993-09-01 00:00:00

  • Membrane immunoglobulin is characterized by distinct structural subpopulations.

    abstract：:Membrane immunoglobulins are integral proteins on B cell surfaces that bind foreign antigens and are critically involved in the regulation of the immune response. Based upon the model of serum IgG, it has been assumed that membrane immunoglobulins are essentially four chain disulfide-linked structures of the form H2L2...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(93)90451-g

    authors： Alhasan S,Santini R,Li Q,Rosenspire A

    更新日期：1993-12-01 00:00:00