Abstract:
:Covalent Superose microspheres-bound C3b was used as a model system to simplify the analysis of antigen-bound C3b modifications during antigen processing. The model was set up using purified C3 and Superose-bound trypsin. C3b was covalently bound to Superose through an ester link, as indicated by lability to hydroxylamine treatment at alkaline pH. C3b-Superose was incubated with L subcellular fraction, enriched in endosomes/lysosomes, purified from U937 cell line. Two types of limited activities on the C3b-Superose model system were detected: (i) a proteolytic activity cleaving C3b into mainly a C3c-like fragment which was released and a C3d-like fragment of apparent M(r) 32 kDa which remained bound to Superose through the original ester link; (ii) an esterolytic activity cleaving the ester bond and releasing C3b. Inhibition experiments pointed to the involvement of serine, aspartyl and cysteine proteases. Cathepsin B appeared most probably as one of the major proteases of L fraction catalysing the proteolysis of the C3b-bound. Kinetic studies were in favour of a good stability on the ester bond, supporting an effective role of C3b as a chaperone during the extracellular and intracellular travel of C3b-bound antigen.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Rey-Millet CA,Chesne S,Colomb MGdoi
10.1016/0161-5890(93)90009-zsubject
Has Abstractpub_date
1993-07-01 00:00:00pages
855-64issue
10eissn
0161-5890issn
1872-9142journal_volume
30pub_type
杂志文章abstract:BACKGROUND AND OBJECTIVES:The complement system plays an important role in the development of acute coronary syndrome (ACS). Complement C1q is an important initial component of the classical complement pathway and closely related to many chronic inflammatory diseases, including atherosclerosis (AS). We aimed to determi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.02.012
更新日期:2020-04-01 00:00:00
abstract::The analysis of the antigenic structure of human interferon (IFN)-alpha 1 with a panel of monoclonal antibodies revealed four immunodominant regions. Three of them, recognized by 12 of 14 antibodies were mapped into the aminoterminal portion of IFN-alpha 1 around residues 31-38, 43-53 and 63-85. The region 31-85 prove...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90124-g
更新日期:1992-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.molimm.2018.11.004
更新日期:2019-01-01 00:00:00
abstract::The architecture of lymphoid microenvironments depends upon complex interactions between several stromal cell types. We describe in this report the cloning of a cDNA which encodes a novel membrane molecule containing two external Ig-like domains. It is expressed at the junction between endothelial cells including HEV....
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(98)00102-3
更新日期:1998-12-01 00:00:00
abstract:BACKGROUND:Allergen epitope characterization provides valuable information useful for the understanding of proteins as food allergens. It is believed that IgE epitopes in general are conformational, nevertheless, for food allergens known to sensitize through the gastrointestinal tract linear epitopes have been suggeste...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.04.002
更新日期:2012-07-01 00:00:00
abstract::Using the non-crossreactive mAb MRC-OX3 and MRC-OX6, two serologically distinct RT1.B-specific (I-A equivalent) alpha, beta heterodimers have previously been described by us as residing at the cell surface of LEW rat spleen cells. The two-chain elements were suggested to represent stable conformation isomers, diverged...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90099-0
更新日期:1989-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(86)90163-x
更新日期:1986-11-01 00:00:00
abstract::Complement per se has been shown to play an important role in demyelinating disease but controversy remains regarding the role of C3 in the development and progression of experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis. In this study, we used C3(-/-) mice to confirm previous fi...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.02.002
更新日期:2007-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90187-8
更新日期:1987-08-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(94)00150-y
更新日期:1995-03-01 00:00:00
abstract::Mastocytosis is an incurable and sometimes fatal haematological disorder grossly described as the accumulation of abnormal mast cells in the bone marrow and other organs causing tissue and organ damage. The clinical manifestations of this disease are extremely variable; disease phenotypes range from indolent to aggres...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2014.03.013
更新日期:2015-01-01 00:00:00
abstract::Tremendous advances in our understanding of the thrombotic microangiopathies (TMAs) have revealed distinct disease mechanisms within this heterogeneous group of diseases. As a direct result of this knowledge, both children and adults with complement-mediated TMA now enjoy higher expectations for long-term health. In t...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2015.03.246
更新日期:2015-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.01.015
更新日期:2009-05-01 00:00:00
abstract::The concomitant effects of trans-chromosomal allotype suppression of both an a VH and an n Cmu locus allotype in multiheterozygous rabbits were investigated. For example of the expression of the a2 VH and n81 Cmu allotypes were suppressed in a multiheterozygous rabbit having the a1 chi-y-n81de12,15f73g74/a2 chi 32y33n...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(82)90257-7
更新日期:1982-01-01 00:00:00
abstract::Myeloid differentiation protein 2 (MD-2) is required in the recognition of lipopolysaccharide (LPS) by toll-like receptor 4 (TLR4), and participates in LPS-induced alveolar macrophage (AM) inflammation during acute lung injury (ALI). Activation of the NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflamm...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.06.035
更新日期:2017-10-01 00:00:00
abstract::The outer membrane protein A (OmpA) is one of the intra-species conserved proteins with immunogenicity widely found in the family of Enterobacteriaceae. Here we first confirmed OmpA is conserved in the three pathogenic Yersinia: Yersinia pestis, Yersinia pseudotuberculosis and pathogenic Yersinia enterocolitica, with ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2015.09.016
更新日期:2015-12-01 00:00:00
abstract::Immunoglobulins are important elements of the adaptive immune system that bind to an immense variety of microbial antigens to neutralize infectivity and specify effector functions. In the present study, the immunoglobulin heavy chain constant region (IGHC) genes from marine mammals were identified and compared with th...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2018.04.011
更新日期:2018-07-01 00:00:00
abstract::Inhibition of the catalytic activity of horseradish peroxidase by rabbit antisera was measured using alternate enzyme substrates. The same general inhibition curve patterns were obtained regardless of the electron donor or hydroperoxide used. In all cases typical biphasic inhibition patterns were found and complete in...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90169-4
更新日期:1983-12-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.12.004
更新日期:2009-04-01 00:00:00
abstract::In a previous study we isolated a series of rat monoclonal antibodies to the human leukocyte common (LC) antigen and demonstrated that synergistic complement lysis was possible between IgG2b antibodies which recognised different epitopes. In this report we have examined the mechanisms that were involved in synergistic...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90039-3
更新日期:1987-06-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(02)00117-7
更新日期:2002-11-01 00:00:00
abstract::It is well established that many cell functions are controlled by the PI-3K signaling pathway and the signaling lipid, phosphatidylinositol-3,4,5-triphosphate (PIP3). This is particularly true for mast cells which play a key regulatory role in allergy and inflammation through activation via high-affinity IgE receptors...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.08.009
更新日期:2010-11-01 00:00:00
abstract::Most of the complement proteins in circulation are, by and large, synthesized in the liver. However data accumulated over the past several decades provide incontrovertible evidence that some if not most of the individual complement proteins are also synthesized extrahepatically by activated as well as non-activated ce...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2016.11.003
更新日期:2017-04-01 00:00:00
abstract::Macrophages (Mφ) undergo activation to pro-inflammatory (M1) or anti-inflammatory (M2) phenotypes in response to pathophysiologic stimuli and dysregulation of the M1-M2 balance is often associated with diseases. Therefore, studying mechanisms of macrophage polarization may reveal new drug targets. Human Mφ polarizatio...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.05.027
更新日期:2017-08-01 00:00:00
abstract::This paper describes additional structural analyses of the p24 cell surface molecule recognized by monoclonal antibody BA-2. Since BA-2 is broadly reactive with a variety of normal and malignant lymphohematopoietic and nonlymphohematopoietic cells, we examined the structure of p24 expressed on different cell types. Tr...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90007-0
更新日期:1985-10-01 00:00:00
abstract::Bifunctional antigens composed of one L-tyrosine-p-azobenzenearsonate (Tyr-ABA) carrier epitope and one dinitrophenyl (DNP) haptenic epitope separated by 6-aminocaproyl or polyprolyl spacers induced weak IgM anti-DNP plaque-forming cell (PFC) responses in the spleens of mice immunized intraperitoneally, without detect...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(84)90069-5
更新日期:1984-06-01 00:00:00
abstract::Grass pollens are amongst the most important aeroallergen sources world-wide triggering allergic rhinoconjunctivitis and asthma in sensitised patients. Much of what we know about the allergen components of grasses is informed by research on pollen of temperate (Pooideae) species that are abundant in the temperate clim...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2018.03.012
更新日期:2018-08-01 00:00:00
abstract::Prolactin has several immune functions in fish however, the effects on innate and specific components of rainbow trout immunity are currently unknown. Therefore in this study, prolactin peptide (pPRL) injection in rainbow trout generated anti-PRL antibodies that were confirmed through Western blot assays of fish brain...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.08.020
更新日期:2020-11-01 00:00:00
abstract::The 3-D complex structures of extracellular domain of human CD16 (FcgammaRIII) and hIgG1 or B88-9 were obtained by means of computer-guided molecular modeling, respectively. The binding epitopes were predicted and binding energy was calculated theoretically. The epitopes of human CD16 interacted with hIgG1 and B88-9 w...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.03.024
更新日期:2004-05-01 00:00:00
abstract::NKG2D receptor-ligand interaction triggers NK cell-mediated cytolysis and IFN-gamma secretion. IFN-gamma produced by NK cells has been found to promote the interaction between NK cells and monocytes; however, the underlying mechanism remains elusive. We demonstrate here that IFN-gamma exclusively induced or upregulate...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.10.007
更新日期:2008-03-01 00:00:00