Abstract：
:Aging is an irreversible physiological process characterized by increased risk of diseases, reduced effectiveness of vaccines, and decreased immune responses. One of the most prominent paradigms of aging and age related conditions is the progressive accumulation of un-repaired DNA breaks leading to apoptosis and exhaustion of stem cells. Here we hypothesized that B lymphocytes from old mice have reduced DNA repair mechanisms as a contributing factor for DNA break accumulation. We analyzed class switch recombination (CSR) of naïve B lymphocytes from old and adult mice to delineate the DNA double strand repair mechanisms during aging. In vitro CSR assays and DNA break analysis by labeling phosphorylated histone H2AX showed that old mice had significantly reduced DNA repair efficiency following DNA breaks. Functional efficiency analysis of DNA break repairs using plasmid ligation method showed that B lymphocytes from old mice had poor repair efficiency and increased misrepair of linear plasmid. Diminished DNA repair in old age can contribute to reduced immune cell repertoire and impaired immunity; increased occurrence of cancer; and reduced stem cell reserve.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Puthiyaveetil AG,Caudell DLdoi
10.1016/j.molimm.2012.07.001subject
Has Abstractpub_date
2013-01-01 00:00:00pages
79-87issue
1-2eissn
0161-5890issn
1872-9142pii
S0161-5890(12)00347-1journal_volume
53pub_type
杂志文章abstract：:The innate immune system is the first line of defense against infection and responses are initiated by pattern recognition receptors (PRRs) that detect pathogen-associated molecular patterns (PAMPs). PRRs also detect endogenous danger-associated molecular patterns (DAMPs) that are released by damaged or dying cells. T...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2017.02.012
更新日期：2017-06-01 00:00:00
abstract：OBJECTIVE:Nuclear components targeted by autoantibodies are a characteristic feature of the autoimmune disease systemic lupus erythematosus (SLE). The nucleosome, a major autoantigen, is released in patients with SLE as a result of a disturbed apoptosis and/or an insufficient clearance of apoptotic debris. During apopt...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.08.003
更新日期：2010-11-01 00:00:00
abstract：:Dendritic cells (DCs) serve to maintain peripheral tolerance under steady state conditions. Upon triggering by activation signals they initiate strong immune responses. The activation of DCs is accompanied by a rapid upregulation of proinflammatory cytokines, which were shown in other cell types to be regulated by mec...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.11.002
更新日期：2010-02-01 00:00:00
abstract：:Structurally diverse dextrans from Leuconostoc mesenteroides and related bacteria have been used extensively in fundamental immunochemical studies such as induction and characterization of anti-dextran antibodies, as well as in studies of their interaction with pneumococcal antisera, normal bovine serum, concanavalin ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/0161-5890(86)90131-8
更新日期：1986-09-01 00:00:00
abstract：:The lymphocyte antigen CD6 has been extensively studied in humans, but has not been reported in mouse. Here we describe the isolation and characterization of a cDNA clone encoding the murine homologue of the human lymphocyte antigen CD6. Comparison of the predicted amino acid sequence of human and murine CD6 shows tha...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)00166-x
更新日期：1995-01-01 00:00:00
abstract：:Mammalian cells produce inflammatory cytokines and chemokines in response to innate immune signals and their expression is tightly regulated. Chemokine (C-X-C motif) ligand 2 (CXCL2), also known as macrophage inflammatory protein 2-alpha (MIP2-alpha), is an inflammatory chemokine belonging to the CXC chemokine family....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.11.007
更新日期：2017-01-01 00:00:00
abstract：:Immunoglobulins (Ig) require correct folding and assembly of both heavy (H) and light (L) chains to form a functional H2L2 dimer that is secreted from plasma cells. This process is dependent upon the endoplasmic reticulum (ER) chaperone BiP, which targets improperly, folded or assembled Ig molecules for degradation. W...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.11.015
更新日期：2005-05-01 00:00:00
abstract：:Endodontic infection is a widespread oral problem. DNA methylation is a key epigenetic modification that plays important roles in various inflammatory responses, but its role in dental pulp inflammation is poorly understood. In this study, we assessed the expression of DNA methyltransferases (DNMTs) in human dental pul...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.03.012
更新日期：2019-07-01 00:00:00
abstract：:Human C3b as well as native C3 were found to bind to solid phase human and rabbit IgG. Haemolytically active C3 had significantly higher binding capacity to IgG than the C3b fragment. Inhibition experiments proved that C3 and C3b have common binding sites on the Fab and Fc part of the IgG molecule but the character of...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90076-7
更新日期：1983-08-01 00:00:00
abstract：:The role of secondary structures and base mutability at different levels of transcription and supercoiling is analyzed in variable region antibody genes VH5, VH94 and VH186.2. The data are consistent with a model of somatic hypermutation in which increasing levels of transcription and secondary structure stability cor...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.05.012
更新日期：2008-08-01 00:00:00
abstract：:Revascularization of an amputated limb within 4-6h is essential to avoid extensive ischemia/reperfusion (I/R) injury leading to vascular leakage, edema and tissue necrosis. I/R injury is a pathological inflammatory condition that occurs during reperfusion of an organ or tissue after prolonged ischemia. It is character...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.06.021
更新日期：2017-08-01 00:00:00
abstract：:The anti-cytokeratin (CK) 8 monoclonal antibody (mab) TS1 has been shown to efficiently bind to CK8 expressed in carcinomas in vivo. The anti-idiotypic antibody of TS1, alphaTS1, can be used to regulate the tumor:non-tumor ratio of TS1 by clearing non-tumor binding TS1 from the circulation. If the interaction of TS1 t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.08.001
更新日期：2007-02-01 00:00:00
abstract：:The role of glycosylation in membrane expression and secretion of IgM and IgA was investigated in murine lymphoma and hybridoma cell lines, derived from I.29 tumor, which synthesize IgM or IgA with identical variable regions. Tunicamycin, a selective inhibitor of N-linked glycosylation, prevented the membrane expressi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90023-3
更新日期：1984-08-01 00:00:00
abstract：:We investigated the antibody (Ab) and cytotoxic T lymphocyte (CTL) responses to gene gun (GG)-mediated DNA immunization via the intramuscular (i.m.) and intradermal (i.d.) routes. BALB/c mice were immunized five times at weekly intervals with plasmid DNA encoding enhanced green fluorescent protein (EGFP). EGFP product...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(03)00024-5
更新日期：2003-05-01 00:00:00
abstract：:Ig production by splenic human B cells that express different surface Ig isotypes were analysed in limiting dilution cultures. Therefore, FACS sorted IgM+, IgG+ and IgA1+ B cells were stimulated with PMA-activated EL4 thymoma cells as helper cells in the presence of IL-2 and IL-4. We found that at least every second B...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90051-x
更新日期：1992-10-01 00:00:00
abstract：:Human promyelocytic leukemia (HL-60) cells were induced by 1,25-dihydroxyvitamin D3 (calcitriol) to differentiate and examined using a panel of monoclonal antibodies (MoAbs) and functional assays. Although morphologically and histochemically these cells appeared to be of the monocyte-macrophage phenotype, there was a ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90180-4
更新日期：1985-05-01 00:00:00
abstract：:A new sulfhydryl redox model of antibody-dependent endocytosis is proposed. This model synthesizes many previous studies employing a variety of experimental approaches. Predictions of this model can be tested experimentally. ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90088-4
更新日期：1985-08-01 00:00:00
abstract：:This study was undertaken to investigate the nature and microheterogeneity of the carbohydrate moiety of the Fc epsilon receptors of RBL-CA10 and RBL-CA10.7 cells. Treatment using the glycosylation processing inhibitors, castanospermine (CN), 1-deoxymannojirimycin (DMJ), and swainsonine (SW) resulted in a decrease of ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90060-o
更新日期：1993-02-01 00:00:00
abstract：:Toll-like receptors (TLR) are expressed on the surface or intracellularly by dendritic cells (DC) and recognize specifically different pathogen-associated molecular patterns (PAMPs). Increasing evidence suggests that TLR expressed by DC can cooperate to synergize their functions. Here, we describe the cooperation of T...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.02.021
更新日期：2008-01-01 00:00:00
abstract：:When 5-fluorouracil (5-FU) resistant bone marrow (BM) cells are depleted of B-cells and then cultured in insert chambers [separated from a layer of adherent BM (aBM) cells by a nucleopore membrane], no mature, lipopolysaccharide (LPS) reactive B-cells are formed. Factors acting on B-cell precursors are not produced un...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90146-0
更新日期：1988-11-01 00:00:00
abstract：:Maspin (mammary serine protease inhibitor) is a non-inhibitory member of the serine protease inhibitor superfamily and a tumor suppressor in several cancers due to its ability to inhibit cell invasion, angiogenesis, and promote apoptosis. However, its immunomodulatory function remains largely unexplored. Thus, we expl...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.12.021
更新日期：2017-02-01 00:00:00
abstract：:Concanavalin A (Con A)section-binding proteins from mouse spleen leukocytes were characterised by two-dimensional electrophoresis of material precipitated, by Con A plus anti-Con A, from lysates of biosynthetically-labelled cells. Although most cell surface (iodinatable) proteins are known to bind Con A, some of the m...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90030-5
更新日期：1983-04-01 00:00:00
abstract：:In the present study, we report the activation of murine peritoneal macrophages in vitro on irradiation with sublethal dose of UVB (50 mJ/cm(2)). The activation involves enhanced expression of CD18 molecule and production of nitric oxide (NO), tumor necrosis factor (TNF-alpha) and interleukin-1 (IL-1). Production of N...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.01.001
更新日期：2004-04-01 00:00:00
abstract：:Evidence has been accumulating for a role of inflammation in the development of Alzheimer's disease (AD), a progressive neurodegenerative disorder causing a common form of dementia in the elderly. C1q, part of the initiation component of the classical complement pathway (CCP), is associated with beta-sheet, fibrillar ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.02.022
更新日期：2008-06-01 00:00:00
abstract：:Chlorophyllin (CHL) was earlier shown to reduce the level of intracellular ROS and apoptosis induced by ionizing radiation and 2,2'-azobis(2-propionimidinedihydrochloride) (AAPH). In the present studies, the effect of CHL on radiation-induced immunosuppression and modulation of immune responses in mice was examined. C...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.02.031
更新日期：2007-01-01 00:00:00
abstract：:T-cell receptor (TCR) repertoire diversity, thymic output, clonal size and peripheral T-lymphocyte numbers largely depend on intra-thymic and post-thymic T-lymphocyte proliferation. However, quantitative insight into thymocyte and T-lymphocyte proliferation is still lacking. We developed a new TCRG-based TCR excision ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.08.019
更新日期：2013-03-01 00:00:00
abstract：:Protein kinase C-associated kinase (PKK; DIK/RIP4) is an ankyrin-repeat containing serine/threonine receptor-interacting protein (RIP)-family kinase that can activate NFkappaB, and is required for keratinocyte development. In earlier studies, the expression of a catalytically inactive mutant of PKK in the B cell linea...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.09.009
更新日期：2006-04-01 00:00:00
abstract：:Macrophages (Mφ) undergo activation to pro-inflammatory (M1) or anti-inflammatory (M2) phenotypes in response to pathophysiologic stimuli and dysregulation of the M1-M2 balance is often associated with diseases. Therefore, studying mechanisms of macrophage polarization may reveal new drug targets. Human Mφ polarizatio...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.05.027
更新日期：2017-08-01 00:00:00
abstract：:The accuracy of calibrated immunoassays for measuring antibody concentration was analysed from a theoretical perspective. The study shows that there are theoretical limits on the accuracy of antibody immunoassays, which are determined by the affinity of the standard and unknown antibodies and the conditions chosen for...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90115-z
更新日期：1991-07-01 00:00:00
abstract：:The mechanism by which heparin inhibits the alternative complement pathway (ACP) by a fluid-phase activator, CoVF, has been studied. Results presented here indicate that heparin's major (rate-limiting) effect on the fluid-phase activation of the ACP was to potentiate Factor H activity. Such an effect results in a very...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90139-6
更新日期：1983-11-01 00:00:00
