当前位置： SCI文献检索 > MOLECULAR IMMUNOLOGY期刊下所有文献 > Characterisation of Concanavalin A-binding glycoproteins from mouse splenic leukocytes by two-dimensional electrophoresis: preferential binding of incompletely glycosylated forms of H-2 antigen to the lectin.

Characterisation of Concanavalin A-binding glycoproteins from mouse splenic leukocytes by two-dimensional electrophoresis: preferential binding of incompletely glycosylated forms of H-2 antigen to the lectin.

Abstract：

:Concanavalin A (Con A)section-binding proteins from mouse spleen leukocytes were characterised by two-dimensional electrophoresis of material precipitated, by Con A plus anti-Con A, from lysates of biosynthetically-labelled cells. Although most cell surface (iodinatable) proteins are known to bind Con A, some of the major Con A-binding proteins detected by immunoprecipitation, after a four-hour biosynthetic labelling period, are not iodinatable and are probably intracellular. Thus the major biosynthetically labelled Con A-binding species are: (i) a non-iodinatable, high molecular weight glycoprotein (C-145); (ii) intracellular precursors of secretory immunoglobulins (IgM and, probably, IgA); (iii) immature (not fully-sialylated) forms of H-2 D and K antigens; and (iv) Ia antigens. In the case of the H-2 antigens, (and possibly of other cell surface proteins) the selection of immature forms by Con A is not due to lack of biosynthetic labelling of mature products, but to preferential binding of Con A to incompletely glycosylated molecules.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Pink JR,Hoessli D,Tartakoff A,Hooghe R

doi

10.1016/0161-5890(83)90030-5

subject

Has Abstract

pub_date

1983-04-01 00:00:00

pages

491-7

issue

4

eissn

0161-5890

issn

1872-9142

journal_volume

20

pub_type

杂志文章

相关文献

MOLECULAR IMMUNOLOGY文献大全
  • Increased PD-1+CD154+ Tfh cells are possibly the most important functional subset of PD-1+ T follicular helper cells in adult patients with minimal change disease.

    abstract：:T follicular helper (Tfh) cells, especially programmed cell death protein 1 (PD-1)+ Tfh cells, exert important functions in the normal immune response. The purpose of this study was to determine the frequency of different subsets of PD-1+ Tfh cells and their functional effects in adult patients with minimal change dis...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2017.12.020

    authors： Li T,Shi Y,Sun W,Wang H,Wang Q,Jiang Y

    更新日期：2018-02-01 00:00:00

  • More efficient peptide binding to MHC class II molecules during cathepsin B digestion of Ii than after Ii release.

    abstract：:The binding of a T cell-presented peptide to MHC class II alpha,beta chains occurs as a concurrent process with the release of the associated invariant chain (Ii) by cathepsin B. Ii was digested by cathepsin B from solubilized, MHC class II alpha,beta,Ii complexes in the presence of N-hydroxysuccinimidyl-4-azidobenzoa...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(94)90122-8

    authors： Daibata M,Xu M,Humphreys RE,Reyes VE

    更新日期：1994-03-01 00:00:00

  • Dysregulation of complement system during pregnancy in patients with preeclampsia: A prospective study.

    abstract：:Recent studies have shown that aberrant activation of the complement system plays an important role in the pathogenesis of preeclampsia. There is evidence to suggest that aberrant activation of the complement system may already be present during the first trimester. Here, we performed a prospective study in which peri...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2020.03.021

    authors： He YD,Xu BN,Wang ML,Wang YQ,Yu F,Chen Q,Zhao MH

    更新日期：2020-04-16 00:00:00

  • The Mycobacterium tuberculosis glycoprotein Rv1016c protein inhibits dendritic cell maturation, and impairs Th1 /Th17 responses during mycobacteria infection.

    abstract：:The myobacterial factors and the associated mechanism by which Mycobacterium tuberculosis (Mtb) evades the host immune surveillance system remain widely unexplored. Here, we found that overexpressing Rv1016c, a mannosylated protein of M. tuberculosis in BCG (rBCG-Rv1016c) led to increased virulence of the recombined B...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2019.02.021

    authors： Su H,Peng B,Zhang Z,Liu Z,Zhang Z

    更新日期：2019-05-01 00:00:00

  • Cytokine profile of CD4+CD25-FoxP3+ T cells in tumor-draining lymph nodes from patients with breast cancer.

    abstract：BACKGROUND:A T cell subtype with the CD4+CD25-FoxP3+ phenotype was recently described. We aimed to investigate the frequency of these cells and their ability to produce cytokines in tumor-draining lymph nodes from patients with breast cancer (BC). MATERIALS AND METHODS:Mononuclear cells from lymph nodes of 20 patients...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2019.10.007

    authors： Niakan A,Faghih Z,Talei AR,Ghaderi A

    更新日期：2019-12-01 00:00:00

  • Polycomb recruitment at the Class II transactivator gene.

    abstract：:The Class II Transactivator (CIITA) is the master regulator of Major Histocompatibility Class II (MHC II) genes. Transcription of CIITA through the IFN-γ inducible CIITA promoter IV (CIITA pIV) during activation is characterized by a decrease in trimethylation of histone H3 lysine 27 (H3K27me3), catalyzed by the histo...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2015.08.003

    authors： Boyd NH,Morgan JE,Greer SF

    更新日期：2015-10-01 00:00:00

  • Immunomodulatory effect of thymoquinone on atopic dermatitis.

    abstract：BACKGROUND:Atopic dermatitis (AD) or atopic eczema is a skin disease characterized with itching, increased serum level of immunoglobulin E, and peripheral eosinophilia. Thymoquinone (TQ) is an important ingredient of Nigella sativa seeds having antioxidant and anti-inflammatory effects. OBJECTIVE:Present study investi...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2018.07.013

    authors： Aslam H,Shahzad M,Shabbir A,Irshad S

    更新日期：2018-09-01 00:00:00

  • Selection of human antibodies against cell surface-associated oligomeric anthrax protective antigen.

    abstract：:The protective antigen (PA(83)) of Bacillus anthracis is the dominant antigen in natural and vaccine-induced immunity to anthrax infection. Three human single-chain variable fragments (scFvs) against cell bound PA were isolated from an antibody phage display library. Specifically, the antibodies were evaluated for the...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2006.11.011

    authors： Steiniger SC,Altobell LJ 3rd,Zhou B,Janda KD

    更新日期：2007-04-01 00:00:00

  • Molecular characterization of six variant Fcgamma receptor class I (CD64) transcripts.

    abstract：:In humans, three distinct but closely related classes of receptors that bind the Fc portion of IgG (FcgammaRI, II and III) have been identified. FcgammaRI can bind monomeric IgG with high affinity and has a unique third extracellular domain (EC3). Three very similar genes have been characterized for FcgammaRI (A, B, C...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/s0161-5890(98)00079-0

    authors： Ernst LK,Duchemin AM,Miller KL,Anderson CL

    更新日期：1998-10-01 00:00:00

  • Isolation and characterisation of Ebolavirus-specific recombinant antibody fragments from murine and shark immune libraries.

    abstract：:Members of the genus Ebolavirus cause fulminating outbreaks of disease in human and non-human primate populations with a mortality rate up to 90%. To facilitate rapid detection of these pathogens in clinical and environmental samples, robust reagents capable of providing sensitive and specific detection are required. ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2011.06.437

    authors： Goodchild SA,Dooley H,Schoepp RJ,Flajnik M,Lonsdale SG

    更新日期：2011-09-01 00:00:00

  • DNA-binding activity of NF-kappaB and phosphorylation of p65 are induced by N-acetylcysteine through phosphatidylinositol (PI) 3-kinase.

    abstract：:N-Acetylcysteine (NAC) has been widely used as an antioxidant in research, however, it has also been found to reduce the binding of TNF to its receptor independent of its antioxidative role. In this study, we investigated the effect of NAC on NF-kappaB activation. In HeLa cells, Hep3B cells, and A549 cells, DNA-bindin...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2008.06.012

    authors： Liu J,Yoshida Y,Yamashita U

    更新日期：2008-09-01 00:00:00

  • Effect of trimming inhibitors on the secretion and biological activity of a murine IgE monoclonal antibody.

    abstract：:Since secretion of IgE antibodies is known to be blocked by tunicamycin, the first aim of the present study was to determine at which step of glycosylation or processing secretion was restored. For this purpose, murine hybridoma cells secreting an anti beta-lactoglobulin IgE were incubated either in the presence of in...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(87)90187-8

    authors： Granato DA,Neeser JR

    更新日期：1987-08-01 00:00:00

  • Toll-like receptor 3 (TLR3) protects retinal pigmented epithelium (RPE) cells from oxidative stress through a STAT3-dependent mechanism.

    abstract：:Toll-like receptors (TLRs) are essential receptors of the innate immune system and are first responders for protection against bacterial and viral pathogens. Recently, several TLRs have also been implicated in regulating cell death and survival in non-pathogen injuries such as stroke and oxidative stress. Investigatin...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2012.11.005

    authors： Patel AK,Hackam AS

    更新日期：2013-06-01 00:00:00

  • Non-traditional roles of complement in type 2 diabetes: Metabolism, insulin secretion and homeostasis.

    abstract：:Type 2 Diabetes (T2D) is a disease of increasing importance and represents a growing burden on global healthcare and human health. In T2D, loss of effectiveness of insulin signaling in peripheral tissues cannot be compensated for by adequate insulin secretion, leading to hyperglycemia and resultant complications. In r...

    journal_title：Molecular immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molimm.2016.12.009

    authors： King BC,Blom AM

    更新日期：2017-04-01 00:00:00

  • Cloning and molecular characterization of a human recombinant IgG Fab binding to the Tat protein of human immunodeficiency virus type 1 (HIV-1) derived from the repertoire of a seronegative patient.

    abstract：:A study aiming at cloning and characterizing natural antibodies to human immunodeficiency virus type 1 (HIV-1) targets is described. In particular, we report the molecular cloning of a Fab molecule binding the HIV-1/Tat protein from a seronegative patient. The Fab was characterized for its binding specificity and inve...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2005.08.003

    authors： Mancini N,Perotti M,Carletti S,Canducci F,Sampaolo M,Clementi M,Burioni R

    更新日期：2006-03-01 00:00:00

  • Molecular analysis of induced idiotypes associated with autoanti-thyroglobulin.

    abstract：:Idiotype (Id) and autoanti-thyroglobulin were induced in different strains of mice by priming with anti-Id to monoclonal anti-thyroglobulins (D8 and G4) and challenged with a subimmunogenic dose of thyroglobulin (Tg). Both D8.Id and G4.Id were induced in CBA mice by priming with the appropriate anti-Id, but only primi...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(85)90159-2

    authors： Male D,Pryce G,Roitt I

    更新日期：1985-03-01 00:00:00

  • Preventive and therapeutic oral administration of the pentacyclic triterpene α,β-amyrin ameliorates dextran sulfate sodium-induced colitis in mice: the relevance of cannabinoid system.

    abstract：:The pentacyclic triterpene α,β-amyrin has been previously reported as an effective compound in the treatment of several inflammatory conditions. Recent evidence indicates that α,β-amyrin displayed its effects through interaction with the cannabinoid pathway. We assessed the anti-inflammatory effects of the α,β-amyrin ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2013.01.018

    authors： Matos I,Bento AF,Marcon R,Claudino RF,Calixto JB

    更新日期：2013-07-01 00:00:00

  • Lactobacillus casei suppresses experimental arthritis by down-regulating T helper 1 effector functions.

    abstract：:Although the beneficial effects of probiotics on wide variety of diseases have been shown, little is known about how probiotics modulate the immune system. In this study we elucidated the underlying mechanisms how Lactobacillus casei (L. casei) protects against rheumatoid arthritis (RA) progression by investigating th...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2007.12.010

    authors： So JS,Kwon HK,Lee CG,Yi HJ,Park JA,Lim SY,Hwang KC,Jeon YH,Im SH

    更新日期：2008-05-01 00:00:00

  • Identification of six novel CC chemokines in gilthead seabream (Sparus aurata) implicated in the antiviral immune response.

    abstract：:Chemokines are key regulators of migration and consequent activation of migrating leukocytes. CC chemokines constitute the largest chemokine group with 24-28 members in mammalian species, and even more in teleost fish, with up to 81 members in zebrafish Danio rerio. Further studies concerning fish chemokine genes will...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2009.12.014

    authors： Cuesta A,Dios S,Figueras A,Novoa B,Esteban MA,Meseguer J,Tafalla C

    更新日期：2010-03-01 00:00:00

  • Serologic and molecular characterization of the T15 idiotype--II. Structural basis of independent idiotope expression on phosphorylcholine-specific monoclonal antibodies.

    abstract：:We examined the expression of T15 idiotopes (Id) on phosphorylcholine (PC)-binding monoclonal immunoglobulins and defined the structural correlates of these Id. The seven monoclonal anti-Id antibodies used as probes recognize distinct determinants that range from the antigen binding site to the CH1 domain on TEPC15. C...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(87)90045-9

    authors： Strickland FM,Gleason JT,Cerny J

    更新日期：1987-06-01 00:00:00

  • Autoreactive B-cell repertoire in mice with chronic graft versus host disease.

    abstract：:A quantitative analysis of the frequencies of autoantibody producing B-cells has been undertaken by producing and analyzing random hybridoma collections generated in fusions with activated B-cells. Activated B-cells were derived from mice injected with LPS and SRBC and normal mice. They were compared to those derived ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(88)90159-9

    authors： Rolink AG,Thalmann P,Berger C,Radaszkiewicz T,Melchers F

    更新日期：1988-11-01 00:00:00

  • Divergent roles of histone deacetylase 6 (HDAC6) and histone deacetylase 11 (HDAC11) on the transcriptional regulation of IL10 in antigen presenting cells.

    abstract：:The anti-inflammatory cytokine IL-10 is a key modulator of immune responses. A better understanding of the regulation of this cytokine offers the possibility of tipping the balance of the immune response toward either tolerance, or enhanced immune responses. Histone deacetylases (HDACs) have been widely described as n...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2014.02.019

    authors： Cheng F,Lienlaf M,Perez-Villarroel P,Wang HW,Lee C,Woan K,Woods D,Knox T,Bergman J,Pinilla-Ibarz J,Kozikowski A,Seto E,Sotomayor EM,Villagra A

    更新日期：2014-07-01 00:00:00

  • Endogenous ligands of natural killer T cells are alpha-linked glycosylceramides.

    abstract：:The nature of the endogenous ligands for natural killer T (NKT) cells has been debated for more than a decade. Because the mammalian glycosylceramide synthases are invertases, it is believed that in mammals all glycosylceramides are β anomers. However, the possibility that an alternative enzymatic pathway, an unfaithf...

    journal_title：Molecular immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.molimm.2015.06.009

    authors： Kain L,Costanzo A,Webb B,Holt M,Bendelac A,Savage PB,Teyton L

    更新日期：2015-12-01 00:00:00

  • Characterization of bovine Toll-like receptor 8: ligand specificity, signaling essential sites and dimerization.

    abstract：:Toll-like receptors (TLRs) are a family of highly conserved germline-encoded pattern-recognition receptors (PRRs) and are essential for host immune response. Little is known regarding the activation mechanism of TLRs especially of the TLR7/8/9 subfamily. Here we cloned and characterized bovine TLR8 (bTLR8) and found t...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2008.09.024

    authors： Zhu J,Brownlie R,Liu Q,Babiuk LA,Potter A,Mutwiri GK

    更新日期：2009-02-01 00:00:00

  • Complex regulation of human cathelicidin gene expression: novel splice variants and 5'UTR negative regulatory element.

    abstract：:Cationic antimicrobial peptides play important roles in host defense, linking innate and adaptive immunity. hCAP18, the only human antimicrobial cathelicidin, consists of a conserved N-terminal cathelin-like domain and a C-terminal peptide, LL-37. Expression is regulated during myeloid differentiation, and tightly con...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2007.04.023

    authors： Elloumi HZ,Holland SM

    更新日期：2008-01-01 00:00:00

  • Evolutionary conservation of splice sites in sterile C mu transcripts and of immunoglobulin heavy chain (IgH) enhancer region sequences.

    abstract：:The immunoglobulin JHC mu intron was cloned from genomic DNA of a VHa3 rabbit and a 1257 bp sequence which contains conserved enhancer and splice sites was determined. From positions 315 to 1257, there is approximately 72 and 67% similarity to available sequences of man and mouse, respectively (counting gaps as single...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(89)90119-3

    authors： Mage RG,Newman BA,Harindranath N,Bernstein KE,Becker RS,Knight KL

    更新日期：1989-10-01 00:00:00

  • Characterizing the inhibitory action of zinc oxide nanoparticles on allergic-type mast cell activation.

    abstract：:The development of nanoparticles (NPs) for commercial products is undergoing a dramatic expansion. Many sunscreens and cosmetics now use zinc oxide (ZnO) or titania (TiO2) NPs, which are effective ultraviolet (UV) filters. Zinc oxide topical creams are also used in mild anti-inflammatory treatments. In this study we e...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2015.02.021

    authors： Feltis BN,Elbaz A,Wright PF,Mackay GA,Turney TW,Lopata AL

    更新日期：2015-08-01 00:00:00

  • Biological and molecular docking studies on coagulin-H: Human IL-2 novel natural inhibitor.

    abstract：:Withanolide, coagulin-H (1), was evaluated for its effect on various cellular functions related to immune response including lymphocyte proliferation, and expression of interleukin-2 (IL-2) cytokine, and results were compared with prednisolone (2), a commonly used immune modulating drug. Coagulin-H (1) was found to ha...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2005.10.020

    authors： Mesaik MA,Zaheer-Ul-Haq,Murad S,Ismail Z,Abdullah NR,Gill HK,Atta-Ur-Rahman,Yousaf M,Siddiqui RA,Ahmad A,Choudhary MI

    更新日期：2006-04-01 00:00:00

  • N-substituted benzamides inhibit nuclear factor-kappaB and nuclear factor of activated T cells activity while inducing activator protein 1 activity in T lymphocytes.

    abstract：:N-substituted benzamides are compounds that have recently been reported to inhibit nuclear factor-kappaB (NF-kappaB) activity and induce apoptosis in a pre-B cell line. In this study, we focused on the effects of N-substituted benzamides on transcriptional regulation in Jurkat T cells. We used a model system where the...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/s0161-5890(01)00060-8

    authors： Lindgren H,Pero RW,Ivars F,Leanderson T

    更新日期：2001-08-01 00:00:00

  • Effect of dehydroepiandrosterone on the immune function of mice in vivo and in vitro.

    abstract：:Dehydroepiandrosterone (DHEA) has anti-inflammatory, anti-oxidant and immune-regulating properties, while the mechanism of DHEA actions remains unclear. The present study aims to investigate the effect and possible mechanism of DHEA on immune function of mice in vivo and in vitro. In vivo, a lipopolysaccharide (LPS)-i...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2019.06.004

    authors： Cao J,Yu L,Zhao J,Ma H

    更新日期：2019-08-01 00:00:00