Abstract：
:The protective antigen (PA(83)) of Bacillus anthracis is the dominant antigen in natural and vaccine-induced immunity to anthrax infection. Three human single-chain variable fragments (scFvs) against cell bound PA were isolated from an antibody phage display library. Specifically, the antibodies were evaluated for their ability to bind to cell bound heptameric PA and ultimately protect against the cytotoxicity of lethal toxin. In total, all three scFvs possessed neutralizing activity against the cytotoxic effects of lethal toxin in a macrophage lysis assay. The K(d) values of the Fabs were determined, interestingly their protective effects did not parallel their affinities; hence, a simple binding argument alone to PA(63) cannot be used as the distinguishing feature for the prediction of their neutralization abilities. Immunofluorescent microscopy experiments were conducted and provided strong evidence for Fab binding to oligomeric PA on the cell surface and thus a plausible mechanism for the toxin neutralization activity that was observed. The results of this study presented herein suggest that our antibodies compete with LF-PA cell surface interactions, and thus may provide potential application of human antibodies as passive immunization prophylactics in cases of B. anthracis exposure and infection.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Steiniger SC,Altobell LJ 3rd,Zhou B,Janda KDdoi
10.1016/j.molimm.2006.11.011subject
Has Abstractpub_date
2007-04-01 00:00:00pages
2749-55issue
10eissn
0161-5890issn
1872-9142pii
S0161-5890(06)00669-9journal_volume
44pub_type
杂志文章abstract：:Inhibition of C5a by antibodies has been demonstrated to dramatically improve survival in various sepsis models in mice and rats. The structural basis of C5a mediated bioactivity and C5a antibody mediated neutralization are of interesting to be investigated. In the previous study, we obtained a novel functional mouse ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.03.013
更新日期：2011-07-01 00:00:00
abstract：:The major product of digestion of bovine IgG2a(A1) with immobilized pepsin is F(ab')2 while similar treatment of IgG2a(A2) yields Fab, Fc and other products. It is postulated that structural differences in the hinge region, including the absence of the most N-terminal disulfide bridge in IgG2a(A2) and a displacement o...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90128-3
更新日期：1987-12-01 00:00:00
abstract：BACKGROUND:The complement system is well known for its role in innate immunity via the classical, the alternative and the lectin pathways, although recent investigations suggest expanding roles in adipose tissue. Properdin stabilizes C3 convertase following alternative complement activation. Properdin is also present i...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.02.110
更新日期：2012-05-01 00:00:00
abstract：:The structural and functional integrity of the cell is largely maintained by protein-protein interactions. Recently, we demonstrated that multiple antigenic peptides (MAPs) constructed from 60 kDa Ro sequence could be used to show intramolecular and intermolecular protein-protein interaction within the 60 kDa Ro ribon...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.03.036
更新日期：2004-07-01 00:00:00
abstract：:1,25-Dihydroxyvitamin D3 (1,25-D3) is known to have potent inhibitory effects on human peripheral blood mononuclear cell (PBMC) functions. Previous experiments suggest that addition of interleukin-2 (IL-2) to cell cultures can reverse the antiproliferative action of 1,25-D3. Previous studies have also shown that the C...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90064-7
更新日期：1990-01-01 00:00:00
abstract：:In two recent articles published in Molecular Immunology, Steere and colleagues continue their search for an autoimmune mechanism of arthritis in patients who have failed short-course antibiotic therapy for Borrelia burgdorferi, the spirochetal agent of Lyme disease. As in previous attempts, the authors fail to elucid...
journal_title：Molecular immunology
pub_type： 评论,信件
doi：10.1016/j.molimm.2008.02.015
更新日期：2008-06-01 00:00:00
abstract：:Revascularization of an amputated limb within 4-6h is essential to avoid extensive ischemia/reperfusion (I/R) injury leading to vascular leakage, edema and tissue necrosis. I/R injury is a pathological inflammatory condition that occurs during reperfusion of an organ or tissue after prolonged ischemia. It is character...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.06.021
更新日期：2017-08-01 00:00:00
abstract：AIM:To study the role of complement receptor 1 (CR1) for binding of Escherichia coli (E. coli) to erythrocytes, for leukocyte phagocytosis, oxidative burst and complement activation in human whole blood from a CR1 deficient (CR1D) patient and healthy controls with low, medium and high CR1 numbers. METHODS:Alexa-labell...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.07.014
更新日期：2019-10-01 00:00:00
abstract：:Idiotypic-like interactions between mAbs directed against cytidine (Cyd) or guanosine (Guo) nucleosides were characterized. These mAbs, Cyd-1 (IgG2b, kappa), Guo-1 (IgG1, kappa) and Guo-2 (IgG1, kappa) were derived from splenocytes of A/J mice immunized with Cyd-KLH or Guo-KLH and recognized the nucleoside base moieti...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90139-2
更新日期：1994-01-01 00:00:00
abstract：:The interferon (IFN)-inducible p200-protein family includes structurally related murine (for example, p202a, p202b, p204, and Aim2) and human (for example, AIM2 and IFI16) proteins. All proteins in the family share a partially conserved repeat of 200-amino acid residues (also called HIN-200 domain) in the C-terminus. ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2011.11.004
更新日期：2012-01-01 00:00:00
abstract：:We have designed and produced a stable bispecific scFv dimer (bisFv) by non-covalent association of two hybrid VH-VL pairs derived from an anti-neuraminidase antibody (NC10) and an anti-glycophorin antibody (1C3). The bisFv dimer was demonstrated to have binding activity to the two respective target antigens and was e...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(96)00097-1
更新日期：1996-12-01 00:00:00
abstract：:Syngeneic polyclonal antibodies were elicited to an affinity labeled high affinity (2-3 X 10(10) M-1) anti-fluorescein murine IgG2a monoclonal antibody. Hyperimmune ascites fluid was tested for reactivity with homologous liganded, affinity labeled and non-liganded Fab fragments derived from the high affinity antibody....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90111-3
更新日期：1988-08-01 00:00:00
abstract：:N-Acetylcysteine (NAC) has been widely used as an antioxidant in research, however, it has also been found to reduce the binding of TNF to its receptor independent of its antioxidative role. In this study, we investigated the effect of NAC on NF-kappaB activation. In HeLa cells, Hep3B cells, and A549 cells, DNA-bindin...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.06.012
更新日期：2008-09-01 00:00:00
abstract：:Light chains of 426 randomly selected myelomas from BALB/c and NZB mice were characterized by isoelectric focusing (IF) and electrophoresis at pH 3. Approximately 50% of the light chains in each strain were found to have unique electrophoretic properties. The remaining 50% of light chains fell into groups of two or mo...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90040-3
更新日期：1984-05-01 00:00:00
abstract：:Antibodies that recognize Xenopus class II molecules have been developed. Mouse monoclonal antibodies were prepared by immunizing BALB/c mice with frog MHC antigens that had been partially purified with alloantisera, and by immunizing mouse spleen cells in vitro with activated Xenopus T lymphocytes. In addition, five ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90170-5
更新日期：1990-05-01 00:00:00
abstract：:We previously showed that a variety of amino acid substitutions at positions 58 and 59 in the V(H) CDR2 of an anti-arsonate (Ars) antibody Fab simultaneously resulted in increased or unaltered affinity for Ars and substantially enhanced affinity for DNA. To test the generality of these observations, we generated and c...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00005-4
更新日期：1997-12-01 00:00:00
abstract：:Recognition of pathogen-associated molecular patterns (PAMPs) by pattern-recognition receptors (PRRs) is important in innate immune signaling. Toll-like receptors (TLRs) are well-characterized PRRs and are pivotal in antiviral and antitumor host defense. TIR domain-containing adaptor molecule 1 (TICAM-1, also called T...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.03.010
更新日期：2016-05-01 00:00:00
abstract：:BST-2 (also known as tetherin, CD317, or HM1.24) was first described as a potent interferon-inducible host antiviral factor nearly five years ago. Since that time, numerous reports have been published regarding the antiviral activity and immunological properties of this protein. BST-2 blocks viral replication by inhib...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2012.11.008
更新日期：2013-06-01 00:00:00
abstract：:An antimicrobial polypeptide was purified from an acidified gill extract of Pacific oyster (Crassostrea gigas) by C(18) reversed-phase HPLC. The purified polypeptide had a molecular weight of 8471Da containing 74 amino acid residues. Comparison of the obtained N-terminal sequences with those of others revealed that it...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.07.003
更新日期：2013-01-01 00:00:00
abstract：:A set of 58 overlapping rod-bound peptides was used to map the antigenic reactivity pattern of a 64-residue neurotoxin (AaH II) from the venom of the scorpion Androctonus australis hector. Five anti-toxin rabbit antisera were assayed serially for their capacity to bind to each peptide in the set. Six regions of antige...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90152-2
更新日期：1993-08-01 00:00:00
abstract：:The human C epsilon gene produces a number of alternatively spliced heavy chain transcripts of which some encode functional IgE isoforms. We now show that differentially processed epsilon mRNA variants also exist in the mouse and are generated by differential polyadenylation and alternative splicing of primary epsilon...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(96)00110-1
更新日期：1997-02-01 00:00:00
abstract：:Tumor necrosis factor-alpha (TNF) has been implicated in the pathogenesis of a variety of human diseases including septic shock, cachexia, graft-versus-host disease and several autoimmune diseases. Monoclonal antibodies directed against TNF provide an attractive mode of therapeutic intervention in these diseases. We h...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90106-l
更新日期：1993-11-01 00:00:00
abstract：:It is well established that many cell functions are controlled by the PI-3K signaling pathway and the signaling lipid, phosphatidylinositol-3,4,5-triphosphate (PIP3). This is particularly true for mast cells which play a key regulatory role in allergy and inflammation through activation via high-affinity IgE receptors...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.08.009
更新日期：2010-11-01 00:00:00
abstract：:Electron microscopy of specimens of C9 tilted through 90 degrees visualized this protein to be a globular ellipsoid with dimensions of 77 x 70 x 52 A. To check the congruence of this observation with physical properties of the molecule, hydrodynamic parameters for C9 were determined. From this work a frictional ratio ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90156-6
更新日期：1993-08-01 00:00:00
abstract：:Monoclonal antibodies to cyclosporine A (Cs), a potent immunosuppressant, were generated in BALB/c mice using a novel antigen prepared by linking Cs to a protein carrier via a photoactive cross-linking reagent, 4-benzoylbenzoic acid (BBa). Twenty-two monoclonal anti-Cs antibodies were generated, using Cs-BBa-bovine se...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90162-q
更新日期：1992-01-01 00:00:00
abstract：:Ectopic follicles are non-encapsulated organized lymphoid structures that form at sites of inflammation and presumably contribute to the activation and differentiation of cells with autoreactive potential within target tissues. As such, directed targeting of ectopic follicles in settings of autoimmunity may provide a ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.05.006
更新日期：2014-12-01 00:00:00
abstract：:Inhibition of the catalytic activity of horseradish peroxidase by rabbit antisera was measured using alternate enzyme substrates. The same general inhibition curve patterns were obtained regardless of the electron donor or hydroperoxide used. In all cases typical biphasic inhibition patterns were found and complete in...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90169-4
更新日期：1983-12-01 00:00:00
abstract：:The diagnosis and therapy of allergic disorders are usually performed with crude extracts which are a heterogeneous mixture of proteins with different allergenic potency. The knowledge of the allergenic composition is a key step for diagnostic and therapeutic options. Parietaria judaica pollen represents one of the ma...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.01.009
更新日期：2008-05-01 00:00:00
abstract：:Hepcidins are antimicrobial peptides that play important roles in resisting pathogenic infection. Through hybridization of a phage library, the cDNA sequences of three hepcidin-like antimicrobial peptides (named TH1-5, TH2-2, and TH2-3) in tilapia, Oreochromis mossambicus, were determined. The complete hepcidin cDNA s...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.09.031
更新日期：2007-03-01 00:00:00
abstract：:The concomitant effects of trans-chromosomal allotype suppression of both an a VH and an n Cmu locus allotype in multiheterozygous rabbits were investigated. For example of the expression of the a2 VH and n81 Cmu allotypes were suppressed in a multiheterozygous rabbit having the a1 chi-y-n81de12,15f73g74/a2 chi 32y33n...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90257-7
更新日期：1982-01-01 00:00:00
