The heterogeneity of bovine IgG2--IV. Structural differences between IgG2a molecules of the A1 and A2 allotypes.


:The major product of digestion of bovine IgG2a(A1) with immobilized pepsin is F(ab')2 while similar treatment of IgG2a(A2) yields Fab, Fc and other products. It is postulated that structural differences in the hinge region, including the absence of the most N-terminal disulfide bridge in IgG2a(A2) and a displacement of the primary pepsin cleavage site toward the Fd, can explain this effect. The immunodominant A1 allotope(s) appears to be located in the CH3 domain of IgG2a(A1) while the A2 allotopes are located elsewhere and are apparently affected by digestion. The allotypic bias of rabbit anti-IgG2a is also present in anti-IgG2a raised in goats. However, the A2 allotypic determinants of bovine IgG2a are recognized by goat precipitins although precipitins of this specificity are not detectable in rabbits immunized with IgG2a(A2). Rabbit anti-A2 antibodies are detectable using the ELISA in rabbits immunized with IgG2a(A2).


Mol Immunol


Molecular immunology


Heyermann H,Butler JE




Has Abstract


1987-12-01 00:00:00












  • Antigenic sites on the arginine-rich carboxyl-terminal domain of the capsid protein of hepatitis B virus distinct from hepatitis B core or e antigen.

    abstract::The capsid protein of hepatitis B virus (P19) is made of 183 amino acids and carries the antigenic sites of hepatitis B core antigen (HBcAg) and hepatitis B e antigen (HBeAg) on the amino-terminal domain. The carboxyl-terminal domain of P19 (amino acids 150-183) is arginine-rich (47%) and faces the interior of the nuc...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Machida A,Ohnuma H,Takai E,Tsuda F,Tanaka T,Naito M,Munekata E,Miyakawa Y,Mayumi M

    更新日期:1989-04-01 00:00:00

  • The autoimmune TCR-Ob.2F3 can bind to MBP85-99/HLA-DR2 having an unconventional mode as in TCR-Ob.1A12.

    abstract::The generation of T cell receptor (TCR) sequence diversity can produce 'forbidden' clones able to recognize self-antigens. Here, the structure of the complex between a myelin basic protein peptide (MBP85-99), human leukocyte antigen (HLA)-DR2 (DRB1*1501/DRA) and TCR-Ob.2F3, the dominant autoimmune clone obtained from ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Kato Z,Stern JN,Nakamura HK,Miyashita N,Kuwata K,Kondo N,Strominger JL

    更新日期:2010-11-01 00:00:00

  • Tissue distribution, regulation and intracellular localization of murine CD1 molecules.

    abstract::CD1 molecules are MHC-unlinked class Ib molecules consisting of classical (human CD 1a-c) and non-classical subsets (human CD1d and murine CD1). The characterization of non-classical subsets of CD1 is limited due to the lack of reagents. In this study, we have generated two new anti-mouse CD1 monoclonal antibodies, 3H...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Mandal M,Chen XR,Alegre ML,Chiu NM,Chen YH,Castaño AR,Wang CR

    更新日期:1998-06-01 00:00:00

  • Binding of bivalent ligand to cell surface IgE: can one detect ring formation?

    abstract::It is well established that aggregation of cell surface immunoglobulin is involved in signal transduction by cells of the immune system. It is less well understood what special properties of these cell surface aggregates are important in initiating the signal cascade. Several authors have proposed that cells respond t...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Posner RG,Dembo M

    更新日期:1994-12-01 00:00:00

  • Isolation and structural analysis of peptide mimotopes for the disialoganglioside GD2, a neuroblastoma tumor antigen.

    abstract::The disialoganglioside GalAcbeta1-4(NeuAcalpha2-8NeuAcalpha2-3)Galbeta1-4Glcbeta1-1Cer (GD2) is expressed on various tumors, including neuroblastoma, and was defined as a relevant tumor antigen. The monoclonal anti-GD2 antibody 14.18 is widely used for diagnostic purposes in neuroblastoma, and in its mouse/human chime...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Förster-Waldl E,Riemer AB,Dehof AK,Neumann D,Brämswig K,Boltz-Nitulescu G,Pehamberger H,Zielinski CC,Scheiner O,Pollak A,Lode H,Jensen-Jarolim E

    更新日期:2005-02-01 00:00:00

  • Prolactin peptide (pPRL) induces anti-prolactin antibodies, ROS and cortisol but suppresses specific immune responses in rainbow trout.

    abstract::Prolactin has several immune functions in fish however, the effects on innate and specific components of rainbow trout immunity are currently unknown. Therefore in this study, prolactin peptide (pPRL) injection in rainbow trout generated anti-PRL antibodies that were confirmed through Western blot assays of fish brain...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Villalba M,Gómez G,Torres L,Maldonado N,Espiñeira C,Payne G,Vargas-Chacoff L,Figueroa J,Yáñez A,Olavarría VH

    更新日期:2020-11-01 00:00:00

  • The catalytic activity of REV1 is employed during immunoglobulin gene diversification in DT40.

    abstract::REV1 plays a key role in vertebrate translesion synthesis. Although its deoxycytidyl transferase activity is dispensable for tolerance of DNA damage caused by a number of mutagens, its extreme C terminus, which interacts with other translesion polymerases and PCNA, is essential. By examining immunoglobulin diversifica...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Ross AL,Sale JE

    更新日期:2006-04-01 00:00:00

  • A model system for T-lymphocyte differentiation: regulation of CD4 and CD8 gene expression in SL12.4 T-lymphoma cell clones.

    abstract::The T-cell surface proteins CD4 (L3T4) and CD8 (Lyt2) are first expressed on thymocytes as they undergo maturation in the thymus. Two immature T-lymphoma cell clones SL12.4 and RS4.2 which constitutively express low or undetectable levels of CD4 and CD8 were used to investigate the activation of CD4 and CD8 gene expre...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Wilkinson MF,Fong AM,Huynh H,Hays EF,MacLeod CL

    更新日期:1991-01-01 00:00:00

  • An antigenic region of topoisomerase I in DNA polymerase chain reaction-generated fragments recognized by autoantibodies of scleroderma patients.

    abstract::Topoisomerase cDNA and various fragments thereof generated by the DNA polymerase chain reaction were cloned into plasmid expression vectors (pET series) and the expressed polypeptides were probed with scleroderma sera from seven different patients immunoreactive with topoisomerase I. All sera reacted selectively with ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Piccinini G,Cardellini E,Reimer G,Arnett FC,Durban E

    更新日期:1991-04-01 00:00:00

  • Effect of dehydroepiandrosterone on the immune function of mice in vivo and in vitro.

    abstract::Dehydroepiandrosterone (DHEA) has anti-inflammatory, anti-oxidant and immune-regulating properties, while the mechanism of DHEA actions remains unclear. The present study aims to investigate the effect and possible mechanism of DHEA on immune function of mice in vivo and in vitro. In vivo, a lipopolysaccharide (LPS)-i...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Cao J,Yu L,Zhao J,Ma H

    更新日期:2019-08-01 00:00:00

  • Dynamics of measles virus protein expression are reflected in the MHC class I epitope display.

    abstract::Following measles virus (MV) infection, viral peptides are presented to CTL by MHC class I molecules on infected antigen presenting cells at widely different epitope densities. Whereas three MV epitopes (MV-M(211-219), MV-F(438-446) and MV-H(30-38)) derived from different structural proteins occur at regular densities...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Herberts CA,Meiring HM,van Gaans-van den Brink JA,van der Heeft E,Poelen MC,Boog CJ,de Jong AP,van Els CA

    更新日期:2003-01-01 00:00:00

  • Genetics of ankylosing spondylitis.

    abstract::Ankylosing spondylitis (AS) is a chronic inflammatory arthritis that affects the spine and sacroiliac joints. It causes significant disability and is associated with a number of other features including peripheral arthritis, anterior uveitis, psoriasis and inflammatory bowel disease (IBD). Significant progress has bee...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Robinson PC,Brown MA

    更新日期:2014-01-01 00:00:00

  • Isolation and sequence of a cDNA coding for the immunoglobulin mu chain of the sheep.

    abstract::A sheep cDNA library was screened with a human C mu probe, and the complete nucleotide sequence of a 1923 nt cDNA was determined. It contains sequences corresponding to all the exons (VH, DH, JH, CH1, CH2, CH3 and CH4) characteristic of the immunoglobulin mu heavy chain regions. The deduced amino acid sequence shows a...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Patri S,Nau F

    更新日期:1992-07-01 00:00:00

  • Identification and characterization of polymorphisms in the promoter region of the human Apo-1/Fas (CD95) gene.

    abstract::Apo-1/Fas (CD95) is a transmembrane protein expressed on the cell surface that is involved in apoptosis and plays an important role in the function and regulation of the immune system. Aberrant expression of the Apo-1/Fas gene product has been reported in a number of immune-related disorders, such as autoimmune lympho...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Huang QR,Morris D,Manolios N

    更新日期:1997-06-01 00:00:00

  • Major mountain cedar allergen, Jun a 1, contains conformational as well as linear IgE epitopes.

    abstract::We have previously identified four linear IgE epitopes on Jun a 1, the dominant allergen in mountain cedar pollen, and mapped these to the surfaces of a molecular model and to the crystal structure of this glycoprotein. The aim of the present study was to determine if Jun a 1 also displays conformational IgE epitopes....

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Varshney S,Goldblum RM,Kearney C,Watanabe M,Midoro-Horiuti T

    更新日期:2007-04-01 00:00:00

  • Antigen specificity and cross-reactivity of monoclonal anti-lysozyme antibodies.

    abstract::Twenty-seven murine monoclonal anti-hen egg-white lysozyme (HEL) antibodies were tested with a panel of nine antigens; eight avian lysozymes and human lysozyme. The antibodies were arranged into 10 groups based on their antigen specificity and cross-reactivity. Antigenic determinants recognized by each group of antibo...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Harper M,Lema F,Boulot G,Poljak RJ

    更新日期:1987-02-01 00:00:00

  • Correlation of the epitopes defined by anti-CD26 mAbs and CD26 function.

    abstract::To clarify the different anti-CD26 mAbs corresponding different functions of CD26, the correlation of the epitopes defined by anti-CD26 mAbs and the functions of CD26 have been studied. Using truncated, human-rat CD26 swap mutants and cross-blocking studies, 13 anti-CD26 mAbs were divided into 5 separate groups. These...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Dong RP,Tachibana K,Hegen M,Scharpé S,Cho D,Schlossman SF,Morimoto C

    更新日期:1998-01-01 00:00:00

  • Studies of the TLR4-associated protein MD-2 using yeast-display and mutational analyses.

    abstract::Bacterial lipopolysaccharide (LPS) activates the innate immune system by forming a complex with myeloid differentiation factor 2 (MD-2) and Toll-like receptor 4 (TLR4), which is present on antigen presenting cells. MD-2 plays an essential role in this activation of the innate immune system as a member of the ternary c...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Mattis DM,Chervin AS,Ranoa DR,Kelley SL,Tapping RI,Kranz DM

    更新日期:2015-12-01 00:00:00

  • Immunoglobulin G binding activity of Brucella abortus.

    abstract::A Brucella abortus protein with a molecular weight of 50 kDa has been shown to bind bovine immunoglobulin G from healthy, brucellosis-free animals. The Brucella immunoglobulin G binding molecule appears to be a protein, since it is susceptible to proteolysis. The protein is presumed to be located on the cell surface, ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Bricker BJ,Tabatabai LB,Mayfield JE

    更新日期:1991-01-01 00:00:00

  • Alterations in T cell signal transduction by M. leprae antigens is associated with downregulation of second messengers PKC, calcium, calcineurin, MAPK and various transcription factors in leprosy patients.

    abstract::Mycobacterium leprae, the causative agent of leprosy, challenges host defense mechanism by impairing the signal transduction of T cells which leads to downregulation of T cell proliferation, mainly as a consequence of interference with IL-2 production. In this study we sought to identify how soluble forms of M. leprae...

    journal_title:Molecular immunology

    pub_type: 临床试验,杂志文章


    authors: Chattree V,Khanna N,Rao DN

    更新日期:2007-03-01 00:00:00

  • Molecular characterization, induced expression, and transcriptional regulation of porcine S100A12 gene.

    abstract::The S100A12 gene belongs to the S100 family of genes, which are specific to vertebrates. It is involved in many inflammatory diseases of human and has been considered as a powerful diagnostic gene. In the present study, we identified the porcine S100A12 (pS100A12) gene, provided evidence that pS100A12 is located on ch...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Chen H,Cheng L,Yang S,Liu X,Liu Y,Tang J,Li X,He Q,Zhao S

    更新日期:2010-04-01 00:00:00

  • Lysozymes and lysozyme-like proteins from the fall armyworm, Spodoptera frugiperda.

    abstract::Lysozyme is an important component of the insect non-specific immune response against bacteria that is characterized by its ability to break down bacterial cell-walls. By searching an EST database from the fall armyworm, Spodoptera frugiperda (Negre et al., 2006), we identified five sequences encoding proteins of the ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Chapelle M,Girard PA,Cousserans F,Volkoff NA,Duvic B

    更新日期:2009-12-01 00:00:00

  • Toll-like receptor 3 (TLR3) protects retinal pigmented epithelium (RPE) cells from oxidative stress through a STAT3-dependent mechanism.

    abstract::Toll-like receptors (TLRs) are essential receptors of the innate immune system and are first responders for protection against bacterial and viral pathogens. Recently, several TLRs have also been implicated in regulating cell death and survival in non-pathogen injuries such as stroke and oxidative stress. Investigatin...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Patel AK,Hackam AS

    更新日期:2013-06-01 00:00:00

  • Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthma.

    abstract::The purpose of this study was to evaluate the effects of GATA-6 on airway inflammation and remodeling and the underlying mechanisms in a murine model of chronic asthma. Female BALB/c mice were randomly divided into four groups: phosphate-buffered saline control (PBS), ovalbumin (OVA)-induced asthma group (OVA), OVA+ s...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Fang P,Shi HY,Wu XM,Zhang YH,Zhong YJ,Deng WJ,Zhang YP,Xie M

    更新日期:2016-07-01 00:00:00

  • In vitro activation of murine peritoneal macrophages by ultraviolet B radiation: upregulation of CD18, production of NO, proinflammatory cytokines and a signal transduction pathway.

    abstract::In the present study, we report the activation of murine peritoneal macrophages in vitro on irradiation with sublethal dose of UVB (50 mJ/cm(2)). The activation involves enhanced expression of CD18 molecule and production of nitric oxide (NO), tumor necrosis factor (TNF-alpha) and interleukin-1 (IL-1). Production of N...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Sethi G,Sodhi A

    更新日期:2004-04-01 00:00:00

  • More efficient peptide binding to MHC class II molecules during cathepsin B digestion of Ii than after Ii release.

    abstract::The binding of a T cell-presented peptide to MHC class II alpha,beta chains occurs as a concurrent process with the release of the associated invariant chain (Ii) by cathepsin B. Ii was digested by cathepsin B from solubilized, MHC class II alpha,beta,Ii complexes in the presence of N-hydroxysuccinimidyl-4-azidobenzoa...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Daibata M,Xu M,Humphreys RE,Reyes VE

    更新日期:1994-03-01 00:00:00

  • Biosynthesis of the third component of complement by the human monocyte-like cell line, U-937.

    abstract::The human monocyte-like cell line, U-937, was shown to synthesize and secrete C3 by hemolytic assays, radioimmunoassays and metabolic labeling experiments. The daily synthesis of antigenic C3 by unstimulated U-937 cells was low (about 3 ng/10(6) cells/24 hr) over a 3 day period. Induction of the cells to differentiate...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Minta JO,Isenman DE

    更新日期:1987-10-01 00:00:00

  • APRIL promotes proliferation, secretion and invasion of fibroblast-like synoviocyte from rats with adjuvant induced arthritis.

    abstract::Fibroblast-like synoviocyte (FLS) is the ultimate effectual cells in the pathogenesis of rheumatoid arthritis (RA). The current study was undertaken to investigate whether a proliferation-inducing ligand (APRIL) mediates the function of FLS in an animal model of RA. Rat adjuvant-induced arthritis (AA) was induced by i...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Chang Y,Jia X,Sun X,Xu S,Wu Y,Zhang L,Wei W

    更新日期:2015-03-01 00:00:00

  • The N-linked oligosaccharides of the Fc epsilon receptors of rat basophilic leukemia cells.

    abstract::This study was undertaken to investigate the nature and microheterogeneity of the carbohydrate moiety of the Fc epsilon receptors of RBL-CA10 and RBL-CA10.7 cells. Treatment using the glycosylation processing inhibitors, castanospermine (CN), 1-deoxymannojirimycin (DMJ), and swainsonine (SW) resulted in a decrease of ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: LaCroix EL,Froese A

    更新日期:1993-02-01 00:00:00

  • Human myeloma light chains with increased molecular weight: high frequency among lambda chains.

    abstract::The discovery of a human myeloma protein comprising a kappa L-chain with an increased mol. wt of 30,000) (Bouvet et. al., 1980) prompted investigations on the incidence of such heavier L-chains among other human myeloma proteins. In 105 samples examined, 34 were found to have L-chains heavier than normal (23,000-24,00...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Bouvet JP,Pillot J,Liacopoulos P

    更新日期:1983-04-01 00:00:00