A simple procedure for the isolation of human secretory IgA of IgA1 and IgA2 subclass by a jackfruit lectin, jacalin, affinity chromatography.


:Jackfruit lectin, jacalin, prepared from two batches of jackfruit seeds showed a different specificity in precipitating reaction in Agarose gel with various purified immunoglobulins and secretory components. Jacalin-P, extracted from jackfruit seeds from the Philippines, reacts only with serum IgA and secretory IgA of IgA1 subclass. Jacalin-O, extracted from jackfruit seeds from Okinawa prefecture in Japan, makes a strong precipitin are with IgA1 subclass and a weak precipitin arc with IgA2 subclass of IgA2m(2) allotype, IgM, IgD and IgE. Human secretory IgA of IgA1 subclass was isolated from human milk by a single jacalin-P affinity chromatography using D-galactose as a dissociating agent. From conventionally purified human secretory IgA preparation, secretory IgA of IgA1 subclass and of IgA2 subclass were separated from each other. The former was separated as jacalin-P adsorbed fraction and the latter as jacalin-P non-adsorbed fraction by the affinity chromatography. Subclass composition of secretory IgA in human milk was determined by the affinity column and was calculated to be 70% for IgA1 and 30% for IgA2 subclass. Jacalin affinity chromatography has several advantages compared with antibody coupled affinity chromatography, notably, high capacity, inexpensiveness, and very mild extraction of IgA1 subclass.


Mol Immunol


Molecular immunology


Kondoh H,Kobayashi K,Hagiwara K




Has Abstract


1987-11-01 00:00:00












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    authors: Lu XJ,Chen Q,Yang GJ,Chen J

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    authors: Arilla MC,Ibarrola I,Puente Y,Daza JC,Martínez A,Asturias JA

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    authors: Deroo S,Fischer A,Beaupain N,Counson M,Boutonnet N,Pletinckx J,Loverix S,Beirnaert E,De Haard H,Schmit JC,Lasters I

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    authors: Borrego F,Kabat J,Kim DK,Lieto L,Maasho K,Peña J,Solana R,Coligan JE

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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