Abstract：
:Four different proteases were screened for their capability of selectively digesting murine monoclonal IgGl to obtain active F(ab)2. For the screening, a series of five different mouse monoclonal antibodies (IgGl, k) was used, recognizing different tumor-associated antigens and currently used for radioimmunoimaging studies. The enzymes (pepsin, bromelain, ficin and elastase) showed different fragmentation capability and the fragments obtained showed different stability and immunoreactivity. No digestion was noticed using elastase. Pepsin gave discontinuous results, in that its activity ranged from reduction of IgG to small inactive fragments to an inability to digest the immunoglobulin. Pepsin activity was strongly pH-dependent and immunoreactivity of the obtained fragments was not always conserved. Bromelain and, in particular, ficin gave excellent results. Digestion was always rapid and stable, all five MAbs were reduced to F(ab)2 in a comparable time range and with high yields. Moreover, ficin-obtained F(ab)2 showed a highly conserved immunoreactivity. Therefore, ficin was selected as the murine monoclonal IgGl digestion enzyme to obtain active bivalent antibody fragments. The digestion procedure gave a uniform result for all five different MAbs and was easily scaled up to produce hundreds of milligrams of F(ab)2.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Mariani M,Camagna M,Tarditi L,Seccamani Edoi
10.1016/0161-5890(91)90088-2subject
Has Abstractpub_date
1991-01-01 00:00:00pages
69-77issue
1-2eissn
0161-5890issn
1872-9142journal_volume
28pub_type
杂志文章abstract：:This paper describes additional structural analyses of the p24 cell surface molecule recognized by monoclonal antibody BA-2. Since BA-2 is broadly reactive with a variety of normal and malignant lymphohematopoietic and nonlymphohematopoietic cells, we examined the structure of p24 expressed on different cell types. Tr...
journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：1985-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：1996-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：1982-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：1989-03-01 00:00:00
abstract：:The translation of mRNA within total RNA of German (Blattella germanica) cockroaches was performed using a rabbit reticulocyte lysate system. Analysis of the translation products by SDS-PAGE and combined autoradiography revealed several synthesized proteins with apparent molecular weights ranging from 20 kD to 110 kD....
journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：1993-12-01 00:00:00
abstract：:Rabbits hyperimmunized with fluorescyl-conjugated KLH exhibited bound ligand associated with a high affinity circulating IgG anti-fluorescein population. After cessation of immunogen administration the liganded complexes were eventually spontaneously cleared from the circulation. Individual rabbits synthesized autolog...
journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：1992-02-01 00:00:00
abstract：:The testicular cystic structure and the abrupt morphological changes that the fish testis undergoes during the reproductive cycle (RC) make it an interesting model for studying the regulation of spermatogenesis, in particular the role of matrix metalloproteinases (Mmps). The gilthead seabream is a seasonal breeding te...
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pub_type： 杂志文章
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更新日期：2008-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2000-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2010.12.005
更新日期：2011-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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更新日期：2020-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2002-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90130-2
更新日期：1989-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(02)00142-6
更新日期：2002-11-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2015.04.014
更新日期：2015-12-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,随机对照试验
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更新日期：2019-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.06.006
更新日期：2007-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(98)00094-7
更新日期：1998-11-01 00:00:00
abstract：:A number of studies have showed that High mobility group box-1 (HMGB1), which played key role in inflammation activation by triggering the toll like receptor 4 (TLR4) signaling axis in hepatic fibrogenesis, may share similar inflammation stimulating mechanism with LPS. Herein, we introduced a recently established anti...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.10.018
更新日期：2016-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(02)00117-7
更新日期：2002-11-01 00:00:00
abstract：:Cleavage by the proteasome is responsible for generating the C terminus of T-cell epitopes. Modeling the process of proteasome cleavage as part of a multi-step algorithm for T-cell epitope prediction will reduce the number of non-binders and increase the overall accuracy of the predictive algorithm. Quantitative matri...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.12.013
更新日期：2006-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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更新日期：2015-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90007-6
更新日期：1986-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.11.002
更新日期：2009-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(02)00059-7
更新日期：2002-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.07.010
更新日期：2018-01-01 00:00:00
abstract：UNLABELLED:C5L2, a G-protein-coupled receptor (GPCR), has been identified as an ASP (C3adesArg) and C5a receptor. Controversy exists regarding both ligand binding and functionality. ASP activation of C5L2 is proposed to regulate fat storage. C5L2 is also proposed as a decoy receptor for C5a, an inflammatory mediator, b...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.06.007
更新日期：2009-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.02.001
更新日期：2017-05-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90038-x
更新日期：1983-09-01 00:00:00
abstract：:The number of V alpha and V beta sequences of T cell receptors now available allows a meaningful analysis of their variability profiles. Variability plots were derived using a modified form of Wu and Kabat's algorithm: variability is not computed as a proportion of the number of different residues occurring at a posit...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90178-m
更新日期：1991-09-01 00:00:00
abstract：:T regulatory (Treg)/T-helper (Th) 17 imbalance has been shown to integrate with epigenetics to result in the development of autoimmune diseases. We aim to investigate the influence of disease staging on Treg/Th17 cells and whether the aberrant DNA methylation is implicated in the development of rheumatoid arthritis (R...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2020.08.002
更新日期：2020-11-01 00:00:00
