当前位置： SCI文献检索 > MOLECULAR IMMUNOLOGY期刊下所有文献 > THP-1 and human peripheral blood mononuclear cell-derived macrophages differ in their capacity to polarize in vitro.

THP-1 and human peripheral blood mononuclear cell-derived macrophages differ in their capacity to polarize in vitro.

Abstract：

:Macrophages (Mφ) undergo activation to pro-inflammatory (M1) or anti-inflammatory (M2) phenotypes in response to pathophysiologic stimuli and dysregulation of the M1-M2 balance is often associated with diseases. Therefore, studying mechanisms of macrophage polarization may reveal new drug targets. Human Mφ polarization is generally studied in primary monocyte-derived Mφ (PBMC Mφ) and THP-1-derived Mφ (THP-1 Mφ). We compared the polarization profile of THP-1 Mφ with that of PBMC Mφ to assess the alternative use of THP-1 for polarization studies. Cellular morphology, the expression profiles of 18 genes and 4 cell surface proteins, and phagocytosis capacity for apoptotic cells and S. aureus bioparticles were compared between these Mφ, activated towards M1, M2a, or M2c subsets by stimulation with LPS/IFNγ, IL-4, or IL-10, respectively, for 6h, 24h and 48h. The Mφ types are unique in morphology and basal expression of polarization marker genes, particularly CCL22, in a pre-polarized state, and were differentially sensitive to polarization stimuli. Generally, M1 markers were instantly induced and gradually decreased, while M2 markers were markedly expressed at a later time. Expression profiles of M1 markers were similar between the polarized Mφ types, but M2a cell surface markers demonstrated an IL-4-dependent upregulation only in PBMC Mφ. Polarized THP-1 Mφ but not PBMC Mφ showed distinctive phagocytic capacity for apoptotic cells and bacterial antigens, respectively. In conclusion, our data suggest that THP-1 may be useful for performing studies involving phagocytosis and M1 polarization, rather than M2 polarization.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Shiratori H,Feinweber C,Luckhardt S,Linke B,Resch E,Geisslinger G,Weigert A,Parnham MJ

doi

10.1016/j.molimm.2017.05.027

subject

Has Abstract

pub_date

2017-08-01 00:00:00

pages

58-68

eissn

0161-5890

issn

1872-9142

pii

S0161-5890(17)30161-X

journal_volume

88

pub_type

杂志文章

相关文献

MOLECULAR IMMUNOLOGY文献大全
  • Cloning, sequence analysis and confirmation of derived gene sequences for three epitope-mapped monoclonal antibodies against human phagocyte flavocytochrome b.

    abstract：:The integral membrane protein flavocytochrome b (Cyt b) is the catalytic core of the NADPH oxidase complex, a multicomponent enzyme system that initiates a cascade of reactive oxygen species that play a critical role in innate immunity and vascular physiology. Epitope-mapped, monoclonal antibodies (mAb) that recognize...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2005.10.022

    authors： Taylor RM,Maaty WS,Lord CI,Hamilton T,Burritt JB,Bothner B,Jesaitis AJ

    更新日期：2007-01-01 00:00:00

  • Increased expression of MHC class I molecules on human cells after short time IFN-gamma treatment.

    abstract：:Human cell lines and blood lymphocytes were treated for short time periods with IFN-gamma. This treatment increased the amount of the assembled MHC class I molecules on the plasma membrane after 30 min. This early increase of the membrane expression subsided in the next few hours. A second wave of elevation occurred a...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(93)90008-y

    authors： Végh Z,Wang P,Vánky F,Klein E

    更新日期：1993-06-01 00:00:00

  • Single amino acid substitutions in V(H) CDR2 are sufficient to generate or enhance the specificity of two forms of an anti-arsonate antibody variable region for DNA.

    abstract：:We previously showed that a variety of amino acid substitutions at positions 58 and 59 in the V(H) CDR2 of an anti-arsonate (Ars) antibody Fab simultaneously resulted in increased or unaltered affinity for Ars and substantially enhanced affinity for DNA. To test the generality of these observations, we generated and c...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/s0161-5890(98)00005-4

    authors： Hande S,Manser T

    更新日期：1997-12-01 00:00:00

  • Design, synthesis, and characterization of a 39 amino acid peptide mimic of the main immunogenic region of the Torpedo acetylcholine receptor.

    abstract：:We have designed a 39 amino acid peptide mimic of the conformation-dependent main immunogenic region (MIR) of the Torpedo acetylcholine receptor (TAChR) that joins three discontinuous segments of the Torpedo α-subunit, α(1-12), α(65-79), and α(110 - 115) with two GS linkers: This 39MIR-mimic was expressed in E. coli a...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2014.01.002

    authors： Trinh VB,Foster AJ,Fairclough RH

    更新日期：2014-05-01 00:00:00

  • Organization of immunoglobulin heavy chain constant and joining region genes in the channel catfish.

    abstract：:A channel catfish genomic lambda library was screened with CH and JH probes which were derived from our earlier sequence analyses on different full-length heavy chain cDNA clones. One clone, designated C7, contained a genomic insert of about 18 kb and hybridized with specific probes for each of the four domains of the...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(92)90096-g

    authors： Ghaffari SH,Lobb CJ

    更新日期：1992-02-01 00:00:00

  • Mannose-binding lectin is produced by vaginal epithelial cells and its level in the vaginal fluid is influenced by progesterone.

    abstract：:Mannose-binding lectin (MBL) is a recognition molecule of the complement (C) system and binds to carbohydrate ligands present on a wide range of pathogenic bacteria, viruses, fungi, and parasites. MBL has been detected in the cervico-vaginal cavity where it can provide a first-line defence against infectious agents co...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2010.07.016

    authors： Bulla R,De Seta F,Radillo O,Agostinis C,Durigutto P,Pellis V,De Santo D,Crovella S,Tedesco F

    更新日期：2010-11-01 00:00:00

  • Effects of IL-4 and Fc gamma receptor II engagement on Egr-1 expression during stimulation of B lymphocytes by membrane immunoglobulin crosslinking.

    abstract：:Egr-1 is an immediate early gene that is rapidly upregulated in response to mitogenic signals induced by antigen receptor crosslinking on murine B lymphocytes. It has been shown that levels of Egr-1 expression are closely correlated with B cell proliferation in several models of B cell activation and tolerance. We com...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(93)90463-l

    authors： Klaus SJ,Phillips NE,Parker DC

    更新日期：1993-11-01 00:00:00

  • Unique biochemical properties of a mutant MHC class I molecule, H-2Ksm1.

    abstract：:This study describes serological and biochemical properties of a novel MHC class I molecule. The mutant H-2Ksm1 molecule was discovered in a mouse because of loss of reactivity of its peripheral blood lymphocytes to monoclonal antibodies. This mutation in the H-2Ks molecule is the first in vivo mutation described that...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(92)90223-k

    authors： Hasenkrug KJ,Kesari KV,Nathenson SG

    更新日期：1992-12-01 00:00:00

  • Regulation of the murine inducible nitric oxide synthase gene by dexamethasone involves a heterogeneous nuclear ribonucleoprotein I (hnRNPI) dependent pathway.

    abstract：:Glucocorticoids down regulate the inducible nitric oxide synthase (iNOS) gene both transcriptionally and post-transcriptionally. The post-transcriptional events are suggested to involve destabilization of the iNOS transcript although the molecular mechanisms for this effect are not known. Recently, our laboratory demo...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2007.01.029

    authors： Söderberg M,Raffalli-Mathieu F,Lang MA

    更新日期：2007-05-01 00:00:00

  • APRIL promotes proliferation, secretion and invasion of fibroblast-like synoviocyte from rats with adjuvant induced arthritis.

    abstract：:Fibroblast-like synoviocyte (FLS) is the ultimate effectual cells in the pathogenesis of rheumatoid arthritis (RA). The current study was undertaken to investigate whether a proliferation-inducing ligand (APRIL) mediates the function of FLS in an animal model of RA. Rat adjuvant-induced arthritis (AA) was induced by i...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2014.11.002

    authors： Chang Y,Jia X,Sun X,Xu S,Wu Y,Zhang L,Wei W

    更新日期：2015-03-01 00:00:00

  • Maspin inhibits macrophage phagocytosis and enhances inflammatory cytokine production via activation of NF-κB signaling.

    abstract：:Maspin (mammary serine protease inhibitor) is a non-inhibitory member of the serine protease inhibitor superfamily and a tumor suppressor in several cancers due to its ability to inhibit cell invasion, angiogenesis, and promote apoptosis. However, its immunomodulatory function remains largely unexplored. Thus, we expl...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2016.12.021

    authors： Wang Y,Sun L,Song Z,Wang D,Bao Y,Li Y

    更新日期：2017-02-01 00:00:00

  • Quasi-elastic light scattering of antigen-antibody complexes.

    abstract：:Many biological properties of immune complexes (IC) depend upon their size. Quasi-elastic light scattering (QLS) was used to measure a mean equivalent hydrodynamic radius (Rh) and variance of the distribution of model IC composed of bovine serum albumin (BSA) as antigen (Ag) and combinations of two or three well-chara...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(88)90086-7

    authors： Yarmush DM,Murphy RM,Colton CK,Fisch M,Yarmush ML

    更新日期：1988-01-01 00:00:00

  • Granulocyte colony stimulating factor (G-CSF) regulates neutrophils infiltration and periodontal tissue destruction in an experimental periodontitis.

    abstract：:Although granulocyte colony-stimulating factor(G-CSF) has pathogenic roles in several immune inflammatory diseases, its role in periodontitis has not been investigated. Here we detected local expression of G-CSF using public datasets in the Gene Expression Omnibus (GEO) database, and immune cell infiltration into ging...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2019.11.003

    authors： Zhang Z,Yuan W,Deng J,Wang D,Zhang T,Peng L,Tian H,Wang Z,Ma J

    更新日期：2020-01-01 00:00:00

  • Primed for success: Oyster parents treated with poly(I:C) produce offspring with enhanced protection against Ostreid herpesvirus type I infection.

    abstract：:The Pacific oyster (Crassostrea gigas) is farmed globally. Ostreid herpesvirus (OsHV-1) causes severe mortalities of farmed C. gigas. Management of OsHV-1 has proven difficult. Oysters treated with poly(I:C) exhibit enhanced protection (EP) against OsHV-1. This chemical treatment is highly effective, but it is not fea...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2016.09.002

    authors： Green TJ,Helbig K,Speck P,Raftos DA

    更新日期：2016-10-01 00:00:00

  • Regulation of human neutrophil guanylate cyclase by metal ions, free radicals and the muscarinic cholinergic receptor.

    abstract：:We have examined the properties of soluble guanylate cyclase activity in the human neutrophil. The enzyme showed complex regulation by metal ions. A 10-fold higher activity was observed in the presence of Mn2+ than Mg2+, while Ca2+ caused an increase in activity only in the presence of Mg2+ ion. Sodium nitroprusside (...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(85)90138-5

    authors： Lad PM,Glovsky MM,Richards JH,Smiley PA,Backstrom B

    更新日期：1985-07-01 00:00:00

  • Functional importance of amino acid residues making up peptide antigenic determinants.

    abstract：:The functional importance of each amino acid residue making up the antigenic determinants of three different peptide-mAb interactions was determined using complete series of substitution analogs of the three immunizing synthetic peptides. Fingerprint substitution profiles for the three different antigenic determinants...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(93)90032-7

    authors： Pinilla C,Appel JR,Houghten RA

    更新日期：1993-04-01 00:00:00

  • Non-small cell lung cancer cells produce a functional set of complement factor I and its soluble cofactors.

    abstract：:The complement system is important for protection from invading pathogens, removal of waste products and guidance of the immune response. Furthermore, complement can be also targeted to cancer cells. However, membrane-bound inhibitors over-expressed by certain types of tumor cells restrict the cytotoxic activity of co...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2007.04.025

    authors： Okroj M,Hsu YF,Ajona D,Pio R,Blom AM

    更新日期：2008-01-01 00:00:00

  • Activation of p38 MAP kinase in T cells facilitates the immune response to the influenza virus.

    abstract：:Activation of p38 MAP kinase in T cells leads to increased interferon-gamma production in CD4+ and CD8+ T cells, and the selective cell death of CD8+ T cells. To address the role of p38 MAP kinase activation in T cells during an in vivo immune response, we examined the response against the influenza virus in transgeni...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/s0161-5890(00)00078-x

    authors： Conze D,Lumsden J,Enslen H,Davis RJ,Le Gros G,Rincón M

    更新日期：2000-06-01 00:00:00

  • Anti-neuroinflammatory effects of 20C from Gastrodia elata via regulating autophagy in LPS-activated BV-2 cells through MAPKs and TLR4/Akt/mTOR signaling pathways.

    abstract：:20C, a novel bibenzyl compound, is isolated from Gastrodia elata. In our previous study, 20C showed protective effects on tunicamycin-induced endoplasmic reticulum stress, rotenone-induced apoptosis and rotenone-induced oxidative damage. However, the anti-neuroinflammatory effect of 20C is still with limited acquainta...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2018.04.014

    authors： Shao QH,Zhang XL,Chen Y,Zhu CG,Shi JG,Yuan YH,Chen NH

    更新日期：2018-07-01 00:00:00

  • Regulation of the Hif-system by micro-RNA 17 and 20a - role during monocyte-to-macrophage differentiation.

    abstract：:MiRNAs are a class of endogenous tiny RNAs that act as inhibitors of translation or promote RNA degradation by duplex-formation within the 3'-UTR of target mRNAs. They play an important role during a wide range of cellular processes by fine-tuning of gene expression. The differentiation of monocytes to macrophages pla...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2013.06.014

    authors： Poitz DM,Augstein A,Gradehand C,Ende G,Schmeisser A,Strasser RH

    更新日期：2013-12-01 00:00:00

  • Evolution of the MHC: antigenicity and unusual tissue distribution of Xenopus (frog) class II molecules.

    abstract：:Antibodies that recognize Xenopus class II molecules have been developed. Mouse monoclonal antibodies were prepared by immunizing BALB/c mice with frog MHC antigens that had been partially purified with alloantisera, and by immunizing mouse spleen cells in vitro with activated Xenopus T lymphocytes. In addition, five ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(90)90170-5

    authors： Flajnik MF,Ferrone S,Cohen N,Du Pasquier L

    更新日期：1990-05-01 00:00:00

  • The difference of binding epitopes on human CD16 (FcgammaRIII) interacted with hIgG1 and monoclonal antibody B88-9.

    abstract：:The 3-D complex structures of extracellular domain of human CD16 (FcgammaRIII) and hIgG1 or B88-9 were obtained by means of computer-guided molecular modeling, respectively. The binding epitopes were predicted and binding energy was calculated theoretically. The epitopes of human CD16 interacted with hIgG1 and B88-9 w...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2004.03.024

    authors： Feng J,Xie Z,Yu M,Guo N,Shen B

    更新日期：2004-05-01 00:00:00

  • The cross-reactivity of rat IgE and IgG with solubilized receptors of rat basophilic leukemia cells.

    abstract：:Normal rat IgG-Sepharose binds the same two receptors from solubilized rat basophilic leukemia (RBL) cells as are bound by rat IgE-Sepharose. These receptors have previously been designated R and H, and have apparent mol. wts of 45,000 and 55,000, respectively. Inhibition studies indicate that, although soluble deaggr...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(82)90274-7

    authors： Kepron MR,Conrad DH,Froese A

    更新日期：1982-12-01 00:00:00

  • Protective KIR-HLA interactions for HCV infection in intravenous drug users.

    abstract：:Intravenous drug use has become the principal route of hepatitis C virus (HCV) transmission due to the sharing of infected needles. In this study, we analyzed the distribution of HLA-KIR genotypes among 160 Puerto Rican intravenous drug users (IDUs) with HCV infection and 92 HCV-negative Puerto Rican IDUs. We found a ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2009.05.014

    authors： Zúñiga J,Romero V,Azocar J,Terreros D,Vargas-Rojas MI,Torres-García D,Jiménez-Alvarez L,Vargas-Alarcón G,Granados-Montiel J,Husain Z,Chung RT,Alper CA,Yunis EJ

    更新日期：2009-08-01 00:00:00

  • Chemotaxis of human monocyte-derived dendritic cells to complement component C1q is mediated by the receptors gC1qR and cC1qR.

    abstract：:Dendritic cells (DCs) are recruited to inflammatory sites where they phagocytose and process antigens for subsequent presentation to the T lymphocytes in the lymphoid tissue. Several leukocyte chemoattractants and their specific receptors have been shown to induce the migration of DC. The complement protein C1q has mu...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2005.07.030

    authors： Vegh Z,Kew RR,Gruber BL,Ghebrehiwet B

    更新日期：2006-03-01 00:00:00

  • BpV(pic) confers neuroprotection by inhibiting M1 microglial polarization and MCP-1 expression in rat traumatic brain injury.

    abstract：:Traumatic brain injury (TBI) is a major cause of motor and cognitive impairment in young adults. It is associated with high mortality rates and very few effective treatment options. Bisperoxovanadium (pyridine-2-carboxyl) [bpV(pic)] is an commercially available inhibitor of Phosphatase and tensin homolog (PTEN). Previ...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2019.04.010

    authors： Liu R,Liao XY,Tang JC,Pan MX,Chen SF,Lu PX,Lu LJ,Zhang ZF,Zou YY,Bu LH,Qin XP,Wan Q

    更新日期：2019-08-01 00:00:00

  • Discovery and expression of 3 siglecs-like in Oreochromis niloticus neutrophil, and their interaction with group B streptococcal sialylated capsular polysaccharides.

    abstract：:Sialic acid - binding immunoglobulin - like lectins (Siglecs) are members of the largest superfamily of immune receptors; they recognize sialic acid and are mainly expressed in immune cells. Studies on mammals indicate that Streptococcus agalactiae (GBS) evade immune reactions by interacting with the host immune cells...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2016.01.005

    authors： Dong J,Wei Y,Ye X,Sun C,Tian Y,Lu M,Du J,Chen Z

    更新日期：2016-05-01 00:00:00

  • Thermodynamic analysis of additivity between the heavy and light chains in affinity maturation of an antibody.

    abstract：:In a recent article published in Molecular Immunology [Furukawa, K., Shimizu, T., Murakami, A., Kono, R., Nakagawa, M., Sagawa, T., Yamato, I., Azuma, T., 2007. Strategy for affinity maturation of an antibody with high evolvability to (4-hydroxy-3-nitrophenyl) acetyl hapten. Mol. Immunol. 44, 2436-2445], the authors m...

    journal_title：Molecular immunology

    pub_type： 评论,信件

    doi：10.1016/j.molimm.2007.07.007

    authors： Kang J,Warren AS

    更新日期：2008-01-01 00:00:00

  • The transcription factor Bright plays a role in marginal zone B lymphocyte development and autoantibody production.

    abstract：:Previous data suggested that constitutive expression of the transcription factor Bright (B cell regulator of immunoglobulin heavy chain transcription), normally tightly regulated during B cell differentiation, was associated with autoantibody production. Here we show that constitutive Bright expression results in skew...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/j.molimm.2011.09.008

    authors： Oldham AL,Miner CA,Wang HC,Webb CF

    更新日期：2011-10-01 00:00:00

  • Comparison of vasoactive intestinal peptide-mediated protein phosphorylation in human lymphoblasts and colonic epithelial cells.

    abstract：:Vasoactive intestinal peptide (VIP) induces phosphorylation of a basic 38,000 mol. wt protein in a human lymphoblastic cell line (Molt 4b) and a human colon carcinoma cell line (HT29). In both cell types, VIP interacts with specific high affinity receptors to activate adenylate cyclase and cAMP-dependent protein kinas...

    journal_title：Molecular immunology

    pub_type： 杂志文章

    doi：10.1016/0161-5890(89)90010-2

    authors： O'Dorisio MS,Campolito LB

    更新日期：1989-06-01 00:00:00