Abstract：
:The complement system is important for protection from invading pathogens, removal of waste products and guidance of the immune response. Furthermore, complement can be also targeted to cancer cells. However, membrane-bound inhibitors over-expressed by certain types of tumor cells restrict the cytotoxic activity of complement. Herein we report that non-small cell lung cancer (NSCLC) cells produce soluble complement inhibitors factor I (FI) and C4b-binding protein (C4BP). FI is a serine protease capable of degrading the activated complement components C3b and C4b, whilst C4BP acts as its cofactor. Furthermore, NSCLC cells express membrane-bound regulators and shed membrane cofactor protein (MCP), which shares cofactor function with C4BP. Secretion of FI from NSCLC cells was higher than previously reported for any non-hepatic source and FI produced by these cells could efficiently support cleavage of C3b and C4b. In vitro functional assays revealed that additional FI significantly decreased C3 deposition and complement-dependent lysis, particularly when cofactors were added. Our results demonstrate that soluble inhibitors produced by NSCLC cells may provide further protection from complement beyond the level ensured by membrane-bound inhibitors and, as such, contribute to the aggressive phenotype of these lung cancer cells.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Okroj M,Hsu YF,Ajona D,Pio R,Blom AMdoi
10.1016/j.molimm.2007.04.025subject
Has Abstractpub_date
2008-01-01 00:00:00pages
169-79issue
1eissn
0161-5890issn
1872-9142pii
S0161-5890(07)00190-3journal_volume
45pub_type
杂志文章abstract：:Integrin-mediated adhesion of human monocytes to fibrinogen regulated by CD11b/CD18 and the closely related integrin CD11c/CD18, play a key role in inflammation. Peripheral blood monocytes isolated from human donors despite expressing CD11c primarily utilized CD11b to mediate adhesion to fibrinogen upon stimulation wi...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.04.021
更新日期：2008-08-01 00:00:00
abstract：:Multilamellar lipid vesicles (MLV) composed of egg yolk lecithin (EYL) suppressed the response of bovine peripheral blood lymphocytes (BPBL) to phytohemagglutinin (PHA). EYL contains 18:1 and 18:2 as the major unsaturated phospholipids. Dioleoyllecithin (DOL; cis 9) MLV did not suppress BPBL blastogenesis. Dilinoeylle...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90195-x
更新日期：1982-11-01 00:00:00
abstract：:It is well established that many cell functions are controlled by the PI-3K signaling pathway and the signaling lipid, phosphatidylinositol-3,4,5-triphosphate (PIP3). This is particularly true for mast cells which play a key regulatory role in allergy and inflammation through activation via high-affinity IgE receptors...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.08.009
更新日期：2010-11-01 00:00:00
abstract：:The collection of epitopes present within the variable regions of the tumor-specific clonal immunoglobulin expressed by B cell lymphomas (idiotype, Id) can serve as a target for active immunotherapy. Traditionally, tumor-derived Id protein is chemically conjugated to the immunogenic foreign carrier protein keyhole lim...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.10.020
更新日期：2009-01-01 00:00:00
abstract：:Glycosylation is an important protein post-translational modification. In this process, the intermediate product, Tn antigen, arises from somatic mutations in core1β3-galactosyltransferase-specific molecular chaperone (Cosmc), which is required for the formation of active core1β3-galactosyltransferase (T-synthase). As...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.06.250
更新日期：2017-10-01 00:00:00
abstract：:The aim of the study was to explore whether fever-range hyperthermia (FRH) might enhance the anticancer and immunoregulatory activities of protein-bound polysaccharides (PBP), a class of fungus derived immunomodifiers used in the cancer adjuvant therapy. Blood lymphocytes and breast cancer cells (MCF-7) were cultured ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.10.013
更新日期：2016-12-01 00:00:00
abstract：:Two anti-phenyloxazolone (phOx3) and one anti-GAT MAbs from C57BL mice are shown to be coded by VH gene 186.2. This gene has been found earlier to code for several anti-NP (NNP) antibodies (Bothwell et al., 1981) and anti-GT antibodies (Rocca-Serra et al., 1983; Carmack and Pincus, 1986). The L chain partner of the VH...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90122-8
更新日期：1988-09-01 00:00:00
abstract：:The ability of bovine IgG preparations to bind to the various distinct human leukocyte Fc gamma receptors was studied. In experiments using intact cells and isolated Fc gamma receptors, it was demonstrated that bovine IgG can bind to Fc gamma receptors of four human cell types but not to Fc gamma receptors of human ne...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90144-1
更新日期：1987-03-01 00:00:00
abstract：:The human C epsilon gene produces a number of alternatively spliced heavy chain transcripts of which some encode functional IgE isoforms. We now show that differentially processed epsilon mRNA variants also exist in the mouse and are generated by differential polyadenylation and alternative splicing of primary epsilon...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(96)00110-1
更新日期：1997-02-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.10.020
更新日期：2006-04-01 00:00:00
abstract：:A monoclonal antibody (mAb) MF30 (IgGl) against human lactate dehydrogenase isoenzyme 5 (HLDH5) was prepared. MF30 was found to bind with high specificity to HLDH5 when the enzyme was adsorbed onto a polystyrene plate but did not recognize the isoenzyme when in solution. The isoenzymes HLDH1, HLDH2 and HLDH3 adsorbed ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90122-7
更新日期：1986-09-01 00:00:00
abstract：:We have recently developed an in vitro culture model enabling the large-scale expansion of switched-memory B lymphocytes, producing a polyclonal human IgG repertoire. Given the importance of glycosylation for the functions of immunoglobulins, we analyzed the N-glycosylation profiles of the immunoglobulin G (IgG) in th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.10.005
更新日期：2014-02-01 00:00:00
abstract：:Adiponectin can protect against inflammation; one of the mechanisms involves direct, inhibition of macrophages (MΦ). We postulated that adiponectin anti-sense transgenic (AsTg) mice raised in our laboratory are prone to inflammation because of systemic low adiponectin levels. The writhing response to acetic acid was u...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.08.015
更新日期：2014-02-01 00:00:00
abstract：:The structural characteristics of antigens recognized by a panel of monoclonal antibodies prepared against a rabbit T-lymphocyte cell line have been investigated. Those antigens which could be isolated using immunoadsorbents prepared from the monoclonal antibodies had mol. wts of 42,000, 90,000 and 120,000. The 42,000...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(84)90094-4
更新日期：1984-01-01 00:00:00
abstract：:Although the etiology of rheumatoid arthritis (RA) remains unknown, it has been widely suggested that RA has a genetic background. In humans, a copy number loss of 22q11.2, a region harboring the VPREB1 gene, has been suggested to be associated with several immunologic disorders, but there has been no study on the cop...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.11.009
更新日期：2011-06-01 00:00:00
abstract：:The purpose of this study was to evaluate the effects of GATA-6 on airway inflammation and remodeling and the underlying mechanisms in a murine model of chronic asthma. Female BALB/c mice were randomly divided into four groups: phosphate-buffered saline control (PBS), ovalbumin (OVA)-induced asthma group (OVA), OVA+ s...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.05.017
更新日期：2016-07-01 00:00:00
abstract：:The transglutaminase 2 (TG2) is one of the enigmatic enzymes with important functional diversity. It plays an important role in several pathologies such as celiac disease (CD). In patients with active CD, the abnormal retrotranscytosis of IgA/gliadin complexes is mediated by Transferrin Receptor 1 (TfR1). This triad a...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2020.02.019
更新日期：2020-05-01 00:00:00
abstract：:The anti-inflammatory cytokine IL-10 is a key modulator of immune responses. A better understanding of the regulation of this cytokine offers the possibility of tipping the balance of the immune response toward either tolerance, or enhanced immune responses. Histone deacetylases (HDACs) have been widely described as n...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.02.019
更新日期：2014-07-01 00:00:00
abstract：:We have designed and produced a stable bispecific scFv dimer (bisFv) by non-covalent association of two hybrid VH-VL pairs derived from an anti-neuraminidase antibody (NC10) and an anti-glycophorin antibody (1C3). The bisFv dimer was demonstrated to have binding activity to the two respective target antigens and was e...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(96)00097-1
更新日期：1996-12-01 00:00:00
abstract：:Extracts of blood lymphocytes, polymorphonuclear neutrophils and B, T or monocytic cell lines were analyzed by two-dimensional gel electrophoresis and immunoradiometric assay after electro-transfer to nitrocellulose sheets with radiolabelled polyclonal or monoclonal antibodies specific for beta 2-microglobulin. Four d...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90032-1
更新日期：1989-08-01 00:00:00
abstract：:To investigate the interaction of DNA and anti-DNA antibodies in the immune complex disease of systemic lupus erythematosus, the fine specificity of binding of a monoclonal anti-DNA antibody was determined. This antibody, termed Cll, was derived from the fusion of spleen cells from an autoimmune MRL-lpr/lpr mouse with...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90364-9
更新日期：1982-05-01 00:00:00
abstract：:Increasing evidence has demonstrated that the epithelial cells in the lung play crucial roles in regulating certain inflammatory responses by modulating Wnt signaling during microbial infection. However, the anti-microbial functions of Wnt signaling in alveolar epithelial cells remain elusive. In this report, we show ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.02.002
更新日期：2014-06-01 00:00:00
abstract：:Toll-like receptors (TLRs) are a family of highly conserved germline-encoded pattern-recognition receptors (PRRs) and are essential for host immune response. Little is known regarding the activation mechanism of TLRs especially of the TLR7/8/9 subfamily. Here we cloned and characterized bovine TLR8 (bTLR8) and found t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.09.024
更新日期：2009-02-01 00:00:00
abstract：:Intercellular adhesion molecule-1 (ICAM-1) functions in leukocyte trafficking, activation, and the formation of the immunological synapse. ICAM-1 is a member of the immunoglobulin superfamily of adhesion proteins, which share a similar structure of repeating Ig-like domains. Many genes in this family, including ICAM-1...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.03.005
更新日期：2010-05-01 00:00:00
abstract：:The antigenic structural features of alpha-lactalbumin have been investigated using a radioimmunoassay, peptide inhibition of the quantitative precipitin reaction, and by immunodiffusion analysis after chemical modification of the molecule. Antigenic activity (in rabbits) was localized to several peptic fragments and ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90192-4
更新日期：1982-11-01 00:00:00
abstract：:The transcription factor NF-kappaB plays critical roles in immune and inflammatory responses. Here we show that filarial parasitic sheath proteins cause activation of NF-kappaB in the airway epithelial HEp-2 cell line. This activation was transient and saturable, and involved degradation of the cytoplasmic inhibitor p...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(00)00039-0
更新日期：2000-02-01 00:00:00
abstract：:The mis-regulation of nuclear factor-kappa B (NF-kappaB) signal pathway is involved in a variety of inflammatory diseases that leds to the production of inflammatory mediators. Our studies using human U937 promonocytes cells suggested that magnolol, a low molecular weight lignan isolated from the medicinal plant Magno...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.12.004
更新日期：2007-04-01 00:00:00
abstract：:Mutation of microphthalmia transcription factor (MITF) results in deafness, bone loss, small eyes, and poorly pigmented eyes and skin. The primary cell types affected in MITF-deficient mice are melanocytes, osteoclasts and mast cells. A search for MITF-associated proteins, using a mast cell library that was screened w...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(02)00059-7
更新日期：2002-09-01 00:00:00
abstract：:The interferon (IFN)-inducible p200-protein family includes structurally related murine (for example, p202a, p202b, p204, and Aim2) and human (for example, AIM2 and IFI16) proteins. All proteins in the family share a partially conserved repeat of 200-amino acid residues (also called HIN-200 domain) in the C-terminus. ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2011.11.004
更新日期：2012-01-01 00:00:00
abstract：:Mangiferin is the major bioactive ingredient in the leaves of Mangifera indica L., Aqueous extract of such leaves have been traditionally used as an indigenous remedy for respiratory diseases including cough and asthma in Traditional Chinese Medicine. Mangiferin was shown to exert its anti-asthmatic effect by modulati...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.07.025
更新日期：2019-10-01 00:00:00
