Abstract:
:The ability of bovine IgG preparations to bind to the various distinct human leukocyte Fc gamma receptors was studied. In experiments using intact cells and isolated Fc gamma receptors, it was demonstrated that bovine IgG can bind to Fc gamma receptors of four human cell types but not to Fc gamma receptors of human neutrophils. 125I-labeled Fc gamma receptors purified on human IgG-Sepharose columns from human B and T lymphocytes, monocytes and eosinophils were able to rebind specifically to insolubilized bovine IgG. In contrast, radioiodinated human neutrophil Fc gamma receptors did not rebind to bovine IgG-Sepharose. A similar pattern of specificity was demonstrated in studies of the binding of 125I-labeled heat-aggregated bovine IgG to various human leukocyte populations. The labeled aggregated bovine IgG bound to peripheral blood mononuclear cells, to B cells from chronic lymphocytic leukemia patients and to macrophage-like U-937 cells, but bound poorly to normal human granulocytes. Labeled non-aggregated bovine IgG was not appreciably bound to any of the cell populations. Since bovine IgG in dietary sources is frequently exposed to heat, the effect of heating on the physical state and Fc-binding properties of bovine IgG was examined. The data show that heating bovine IgG at concns of 0.9-3.6 mg/ml at 63 degrees C for 30 min in neutral buffer causes aggregation of bovine immunoglobulin (10-16% aggregation) and increases the ability of bovine IgG preparations to bind to human Fc gamma receptors of intact cells. Gel filtration studies suggest the possibility that bovine IgG may also be aggregated during the pasteurization of raw milk.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Kulczycki A Jrdoi
10.1016/0161-5890(87)90144-1subject
Has Abstractpub_date
1987-03-01 00:00:00pages
259-66issue
3eissn
0161-5890issn
1872-9142journal_volume
24pub_type
杂志文章abstract::Twenty-seven murine monoclonal anti-hen egg-white lysozyme (HEL) antibodies were tested with a panel of nine antigens; eight avian lysozymes and human lysozyme. The antibodies were arranged into 10 groups based on their antigen specificity and cross-reactivity. Antigenic determinants recognized by each group of antibo...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90081-2
更新日期:1987-02-01 00:00:00
abstract::SHP-1 plays an important role for the regulation of signaling from various hematopoietic cell receptors. In this study, we examined IL-3-induced cell proliferation and IL-3 depletion-induced apoptosis in bone marrow-derived mast cells (BMMC) established from motheaten (me) that lack SHP-1 expression, viable motheaten ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.10.001
更新日期:2011-01-01 00:00:00
abstract::Serum levels of IgA1 and IgA2 were measured by solid phase radioimmunoassay in samples from 110 children between 3 months and 10 years of age. Both IgA1 and IgA2 were detectable in all samples, and both IgA1 and IgA2 increased with increasing age. The percent of total serum IgA that was IgA2 did not change with age an...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90038-x
更新日期:1983-09-01 00:00:00
abstract::Innate lymphoid cells (ILCs) contribute to the regulation of gastrointestinal (GI) homeostasis. Over the past 15 years, there has been a large effort to dissect the mechanisms required for GI homeostasis, with a major focus on different immune cell populations and the cytokines that they produce. In contrast to T-help...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2017.11.013
更新日期:2019-06-01 00:00:00
abstract::Monoclonal antibodies (MAbs) raised against trout immunoglobulin (Ig) recognize two independent Ig populations differing in the molecular weight of L chains. The trout Ig population recognized by MAb 2H9 represented congruent to 20% of the total Ig and contained L chains congruent to 26 kDa, whereas the trout Ig popul...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(91)90014-b
更新日期:1991-11-01 00:00:00
abstract::Monoclonal antibodies to cyclosporine A (Cs), a potent immunosuppressant, were generated in BALB/c mice using a novel antigen prepared by linking Cs to a protein carrier via a photoactive cross-linking reagent, 4-benzoylbenzoic acid (BBa). Twenty-two monoclonal anti-Cs antibodies were generated, using Cs-BBa-bovine se...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90162-q
更新日期:1992-01-01 00:00:00
abstract::Traumatic brain injury (TBI) is a major cause of motor and cognitive impairment in young adults. It is associated with high mortality rates and very few effective treatment options. Bisperoxovanadium (pyridine-2-carboxyl) [bpV(pic)] is an commercially available inhibitor of Phosphatase and tensin homolog (PTEN). Previ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.04.010
更新日期:2019-08-01 00:00:00
abstract::Degenerate PCR was used to isolate a 221-base pair nucleotide sequence of a new crustin-like antibacterial peptide from the haemocytes of the European lobster, Homarus gammarus. Rapid amplification of cDNA ends was used to extend the sequence to determine the complete open reading frame and un-translated regions. The ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.07.029
更新日期:2006-03-01 00:00:00
abstract::In two recent articles published in Molecular Immunology, Steere and colleagues continue their search for an autoimmune mechanism of arthritis in patients who have failed short-course antibiotic therapy for Borrelia burgdorferi, the spirochetal agent of Lyme disease. As in previous attempts, the authors fail to elucid...
journal_title:Molecular immunology
pub_type: 评论,信件
doi:10.1016/j.molimm.2008.02.015
更新日期:2008-06-01 00:00:00
abstract::Ovalbumin (OVA) is a major allergen (Gal d II) of hen egg white and is often the cause of hypersensitivity reactions to food. Further knowledge of the antigenic and allergenic epitopes of allergens will provide better treatment of this disease. To analyse these epitopes we produced a panel of monoclonal antibodies (mA...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90055-3
更新日期:1992-10-01 00:00:00
abstract::In an attempt to understand the relationship of amino acid sequence to the formation of primary or multiple myeloma-related amyloid (AL amyloid), we have determined the complete amino acid sequence of amyloid protein BAN. This protein belongs to the kappa I immunoglobulin light chain subgroup and has a polypeptide cha...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(86)90173-2
更新日期:1986-01-01 00:00:00
abstract::2-Aminopurine (2-AP) is widely used as an inhibitor for double stranded RNA-dependent protein kinase (PKR). Previously, we reported that 2-AP inhibits Toll-like receptor (TLR) ligand-induced nitric oxide production through the prevention of interferon (IFN)-β production. In this study, we investigated the mechanisms f...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.06.008
更新日期:2012-10-01 00:00:00
abstract::In the previous study, antibodies against CD3 molecule have been produced and were used in labeling T cells in Japanese flounder (Paralichthys olivaceus). In this paper, CD4+ and CD8+ lymphocytes subpopulations in peripheral blood leucocytes (PBL), spleen and head kidney of flounder were investigated. The flounder CD4...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.02.015
更新日期:2017-05-01 00:00:00
abstract::A channel catfish genomic lambda library was screened with CH and JH probes which were derived from our earlier sequence analyses on different full-length heavy chain cDNA clones. One clone, designated C7, contained a genomic insert of about 18 kb and hybridized with specific probes for each of the four domains of the...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90096-g
更新日期:1992-02-01 00:00:00
abstract::UL16 binding proteins (ULBPs) are ligands for the NK cell activating receptor NKG2D. A cDNA encoding a porcine ULBP-like protein (PULBP) was cloned and the predicted amino acid sequence exhibited 35-52% identity to human ULBPs. Southern blot analysis suggested that there is only one ULBP-like gene in the pig genome. T...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.09.020
更新日期:2005-04-01 00:00:00
abstract::The ragweed allergens Amb t 5 and Amb a 5 are among the smallest inhaled protein allergens known, containing a single, immunodominant T-cell epitope. In this study we analyzed the B-cell epitope structure of Amb t 5. The three-dimensional structures of Amb t 5 and Amb a 5 have been determined by NMR spectroscopy, prov...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(99)00054-1
更新日期:1998-06-01 00:00:00
abstract::It is well established that many cell functions are controlled by the PI-3K signaling pathway and the signaling lipid, phosphatidylinositol-3,4,5-triphosphate (PIP3). This is particularly true for mast cells which play a key regulatory role in allergy and inflammation through activation via high-affinity IgE receptors...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.08.009
更新日期:2010-11-01 00:00:00
abstract::In this study we demonstrate that histone deacetylase (HDAC)-inhibitor mediated cell surface expression of the structural different NKG2D-ligands MICA/B and ULBP2 is calcium-dependent. Treatment with the calcium chelator EGTA inhibited constitutive as well as HDAC-inhibitor induced MICA/B and ULBP2 cell surface expres...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.08.011
更新日期:2013-03-01 00:00:00
abstract::Binding of the catecholamine beta-adrenergic antagonist, l-alprenolol, by the IgGl anti-alprenolol monoclonal antibody 37A4 was examined using the radioligand 3H-dihydroalprenolol as an extrinsic signal and the increase in antibody fluorescence upon l-alprenolol binding as intrinsic signal. Equilibrium binding studies...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90043-5
更新日期:1987-06-01 00:00:00
abstract::We showed that nucleotide sequences coding for linear B-cell epitopes of human immunodeficiency virus type 1 (HIV1) gp120 protein are enriched with codons containing cytosine and guanine in their first and second codon positions. Guanine and cytosine are the most mutable nucleotides in HIV1 genes (due to APOBEC3 and A...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.01.006
更新日期:2010-04-01 00:00:00
abstract::Covalent Superose microspheres-bound C3b was used as a model system to simplify the analysis of antigen-bound C3b modifications during antigen processing. The model was set up using purified C3 and Superose-bound trypsin. C3b was covalently bound to Superose through an ester link, as indicated by lability to hydroxyla...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90009-z
更新日期:1993-07-01 00:00:00
abstract::Mammals have evolved a special cellular mechanism for killing invading microbes, which is called the phagocytosis. Neutrophils are the first phagocytosing cells that migrate into the site of infection. In these cells, hypochlorite (HOCl) and other hypohalites, generated in the myeloperoxidase (MPO)-hydrogen peroxide (...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.09.017
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:A T cell subtype with the CD4+CD25-FoxP3+ phenotype was recently described. We aimed to investigate the frequency of these cells and their ability to produce cytokines in tumor-draining lymph nodes from patients with breast cancer (BC). MATERIALS AND METHODS:Mononuclear cells from lymph nodes of 20 patients...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.10.007
更新日期:2019-12-01 00:00:00
abstract::The five-year survival rate of melanoma worsens significantly with advancing tumor stage. We hypothesized that regulatory B cells (Breg) might have participated in the pathogenesis of melanoma. In this study, the PD-L1+ Breg cells were investigated. The expression of PD-L1 by circulating B cells was very low in health...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.01.008
更新日期:2020-03-01 00:00:00
abstract::The T-dependent BALB/c murine immune response to fluorescein (F1) is characterized by structural heterogeneity at the protein level exemplified in part by a significantly wide range of affinities (Ka), and apparent lack of dominant idiotypes. In order to generate an idiotype family, xenogenic anti-idiotype (anti-ID) a...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90072-0
更新日期:1985-08-01 00:00:00
abstract::A number of proteins with significant similarity to the tumour necrosis factor (TNF) have been identified over the last years. Upon interaction with their cognate receptor (members of the TNF-receptor family), all members of this protein family induce either cell death or proliferation/differentiation of the receptor-...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(95)00016-8
更新日期:1995-07-01 00:00:00
abstract::A two-step purification procedure of Par j I from the whole Parietaria judaica pollen extract is described. The first step consisted of gel filtration HPLC using a TSKG 3000 SW column, and 0.1% trifluoroacetic acid as the eluant. By this method, proteins were separated from the highly colored material present in the e...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(90)90110-l
更新日期:1990-02-01 00:00:00
abstract::BST-2 (also known as tetherin, CD317, or HM1.24) was first described as a potent interferon-inducible host antiviral factor nearly five years ago. Since that time, numerous reports have been published regarding the antiviral activity and immunological properties of this protein. BST-2 blocks viral replication by inhib...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2012.11.008
更新日期:2013-06-01 00:00:00
abstract::We examined the expression of T15 idiotopes (Id) on phosphorylcholine (PC)-binding monoclonal immunoglobulins and defined the structural correlates of these Id. The seven monoclonal anti-Id antibodies used as probes recognize distinct determinants that range from the antigen binding site to the CH1 domain on TEPC15. C...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90045-9
更新日期:1987-06-01 00:00:00
abstract::Somatic hypermutation (SHM) in immunoglobulin gene (Ig) variable (V) regions is critical for the maturation of the antibody response. It is dependent on the expression of activation-induced cytidine deaminase (AID) and translesion DNA polymerases in germinal center B cells as well as Ig V transcription, as regulated b...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.10.018
更新日期:2006-04-01 00:00:00