Trout immunoglobulin populations differing in light chains revealed by monoclonal antibodies.

Abstract:

:Monoclonal antibodies (MAbs) raised against trout immunoglobulin (Ig) recognize two independent Ig populations differing in the molecular weight of L chains. The trout Ig population recognized by MAb 2H9 represented congruent to 20% of the total Ig and contained L chains congruent to 26 kDa, whereas the trout Ig population defined by MAb 2A1 comprised 11% of the total Ig and possessed L chains congruent to 24 kDa. The determinants recognized by these MAbs reside on L chains as determined by ELISA using mildly reduced H and L chains. The size heterogeneity of the L chains was not due to differences in glycosylation. Partial peptide maps showed structural differences between both L chains. Both Ig populations were found in each of the 30 trout sera tested. These data extend the existence of L chains diversity in fish, recently described in catfish, to other teleosts.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Sánchez C,Domínguez J

doi

10.1016/0161-5890(91)90014-b

subject

Has Abstract

pub_date

1991-11-01 00:00:00

pages

1271-7

issue

11

eissn

0161-5890

issn

1872-9142

journal_volume

28

pub_type

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