Abstract:
:The human monocyte-like cell line, U-937, was shown to synthesize and secrete C3 by hemolytic assays, radioimmunoassays and metabolic labeling experiments. The daily synthesis of antigenic C3 by unstimulated U-937 cells was low (about 3 ng/10(6) cells/24 hr) over a 3 day period. Induction of the cells to differentiate into macrophage-like cells with phorbol myristate acetate (PMA), resulted in 5-fold augmentation of C3 synthesis and secretion into the culture medium. Using a plaque assay for enumerating C3 production by single cells, approx. 5% of unstimulated U-937 cells were found to secrete hemolytically active C3. The proportion of C3-plaque forming cells was increased about 6-fold in PMA stimulated cells. The synthesis of C3 by U-937 cells was reversibly inhibited by cycloheximide. Data from SDS-PAGE analyses showed that U-937 cells synthesized C3 as a precursor polypeptide chain and was capable of processing this pro-molecule into the secreted two chain form. C3 antigen immunoprecipitated from stimulated U-937 cell lysates showed an increased amount of low mol. wt material as compared to C3 antigen immunoprecipitated from the lysates of unstimulated cells. This may be attributable to increased intracellular proteolytic activity in the PMA stimulated cells. The studies show that the U-937 cell line provides a useful model for studies on the synthesis and processing of complement proteins and the physiological regulation of complement production.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Minta JO,Isenman DEdoi
10.1016/0161-5890(87)90079-4subject
Has Abstractpub_date
1987-10-01 00:00:00pages
1105-11issue
10eissn
0161-5890issn
1872-9142journal_volume
24pub_type
杂志文章abstract::Chemokines are key regulators of migration and consequent activation of migrating leukocytes. CC chemokines constitute the largest chemokine group with 24-28 members in mammalian species, and even more in teleost fish, with up to 81 members in zebrafish Danio rerio. Further studies concerning fish chemokine genes will...
journal_title:Molecular immunology
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journal_title:Molecular immunology
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journal_title:Molecular immunology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1982-11-01 00:00:00
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更新日期:2018-07-01 00:00:00
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更新日期:2011-07-01 00:00:00
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更新日期:2016-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1982-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90032-s
更新日期:1992-03-01 00:00:00
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更新日期:2013-06-01 00:00:00