Abstract：
:SHP-1 plays an important role for the regulation of signaling from various hematopoietic cell receptors. In this study, we examined IL-3-induced cell proliferation and IL-3 depletion-induced apoptosis in bone marrow-derived mast cells (BMMC) established from motheaten (me) that lack SHP-1 expression, viable motheaten (me(v)) expressing phosphatase-deficient SHP-1, and wild-type (WT) mice. When BMMC were stimulated with IL-3, increased ERK activation was evident in resting state and sustained in me-BMMC relative to WT-BMMC. ERK is known to be involved in the regulation of cell proliferation and apoptosis in some cells. In accordance with sustained ERK activation, apoptosis was decreased in me- and me(v)-BMMC compared with WT-BMMC. In contrast to the predicted role of ERK as a pro-survival molecule, IL-3-induced cell proliferation was much lower in me- and me(v)-BMMC than WT-BMMC. Stimulation with lower concentration of IL-3 or addition of PD98059, a MEK inhibitor, to the culture resulted in the suppression of decreased apoptosis and cell proliferation in me- and me(v)-BMMC. Collectively, these results suggest that SHP-1 positively regulates IL-3-dependent mast cell proliferation and apoptosis by inhibiting ERK activity through its phosphatase activity. Furthermore, our results indicate that ERK would act as a negative regulator for cell proliferation and induce apoptosis when its activity is highly increased.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Nakata K,Suzuki Y,Inoue T,Ra C,Yakura H,Mizuno Kdoi
10.1016/j.molimm.2010.10.001subject
Has Abstractpub_date
2011-01-01 00:00:00pages
472-80issue
4eissn
0161-5890issn
1872-9142pii
S0161-5890(10)00581-Xjournal_volume
48pub_type
杂志文章abstract：:Major histocompatibility complex (MHC) class I and II proteins have been in the focus of interest for immunologists, biochemists, cell biologists, and structural biologists for decades. With dozens of entries in the Protein Data Bank, their crystal structures are now sufficiently well understood, while their dynamic p...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2009.10.008
更新日期：2010-01-01 00:00:00
abstract：:Physicochemical techniques were used to verify previous immunochemical studies showing homology of the human Thy-1 (formerly p25) antigen and the murine Thy-1 or theta antigen. Peptide maps and amino acid compositions showed close similarity between these two proteins; however, they were not identical. These data conf...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90216-4
更新日期：1982-03-01 00:00:00
abstract：:The mechanism by which heparin inhibits the alternative complement pathway (ACP) by a fluid-phase activator, CoVF, has been studied. Results presented here indicate that heparin's major (rate-limiting) effect on the fluid-phase activation of the ACP was to potentiate Factor H activity. Such an effect results in a very...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90139-6
更新日期：1983-11-01 00:00:00
abstract：:In most cases, cytotoxic T cell activation is dependent on the interaction of the T cell receptor (TCR) and CD8 with MHC class I molecules. In the CD8(+) T cell system based on the mouse cytotoxic T cell clone 2C, recognition of the allogeneic MHC L(d) exhibits a less significant role for CD8 than recognition of the s...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(02)00259-6
更新日期：2003-01-01 00:00:00
abstract：:Multilamellar lipid vesicles (MLV) composed of egg yolk lecithin (EYL) suppressed the response of bovine peripheral blood lymphocytes (BPBL) to phytohemagglutinin (PHA). EYL contains 18:1 and 18:2 as the major unsaturated phospholipids. Dioleoyllecithin (DOL; cis 9) MLV did not suppress BPBL blastogenesis. Dilinoeylle...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90195-x
更新日期：1982-11-01 00:00:00
abstract：:Peptides representing the amino-terminal sequence of protamines HP1 (sequence 1-12) and HP2 (sequence 1-11), the two major nuclear proteins of human sperm, have been synthesized. Rabbits were immunized either with peptide conjugated with a carrier or with free peptide. The resulting antisera were examined for their ca...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90179-n
更新日期：1991-09-01 00:00:00
abstract：BACKGROUND:Ethanol feeding in mice activates complement via C1q binding to apoptotic cells in the liver; complement contributes to ethanol-induced inflammation and injury. Despite the critical role of C1q in ethanol-induced injury, the mechanism by which ethanol activates C1q remains poorly understood. Secretory IgM (s...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.02.008
更新日期：2016-04-01 00:00:00
abstract：:Chemokines are key regulators of migration and consequent activation of migrating leukocytes. CC chemokines constitute the largest chemokine group with 24-28 members in mammalian species, and even more in teleost fish, with up to 81 members in zebrafish Danio rerio. Further studies concerning fish chemokine genes will...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.12.014
更新日期：2010-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(88)90115-0
更新日期：1988-08-01 00:00:00
abstract：:Evidence for the central role that intestinal beta-defensins play in maintaining gut health continues to accumulate within the literature. Two epithelially-derived enteric beta-defensins, hBD1 and hBD2, have been identified thus far and the following chapter reviews our current understanding of how the expression and ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(03)00161-5
更新日期：2003-11-01 00:00:00
abstract：:The C-terminal domain of protein 4.1G was identified to interact with the cytosolic tail of the high affinity IgG receptor, Fc gamma RI, in yeast two-hybrid screens. Proteins of the 4.1 family have previously been found to mediate receptor/cytoskeleton interactions. In the study presented here, we show an alternativel...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.10.024
更新日期：2008-04-01 00:00:00
abstract：:The mucosal surface of the gastrointestinal tract represents a major entry point and ecological niche for many microbes. It forms an important immune barrier, absorbing nutrients, whilst preventing invasion by organisms. Of the extra-ordinarily diverse species that comprise the microbial world, relatively few organism...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2004.12.004
更新日期：2005-05-01 00:00:00
abstract：:In this study we demonstrate that histone deacetylase (HDAC)-inhibitor mediated cell surface expression of the structural different NKG2D-ligands MICA/B and ULBP2 is calcium-dependent. Treatment with the calcium chelator EGTA inhibited constitutive as well as HDAC-inhibitor induced MICA/B and ULBP2 cell surface expres...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.08.011
更新日期：2013-03-01 00:00:00
abstract：:The complement system is a vital component of the immune-priveliged human eye that is always active at a low-grade level, preventing harmful intraocular injuries caused by accumulation of turnover products and controlling pathogens to preserve eye homeostasis and vision. The complement system is a double-edged sword t...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2017.05.028
更新日期：2017-09-01 00:00:00
abstract：:Although the beneficial effects of probiotics on wide variety of diseases have been shown, little is known about how probiotics modulate the immune system. In this study we elucidated the underlying mechanisms how Lactobacillus casei (L. casei) protects against rheumatoid arthritis (RA) progression by investigating th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.12.010
更新日期：2008-05-01 00:00:00
abstract：:Amyotrophic lateral sclerosis (ALS) is a devastating, neurodegenerative motor neuron disease. The aetiology of ALS remains an enigma which hinders the design of an effective treatment to prevent, postpone, or reverse the pathophysiological changes occurring during the aggressive progression of this disease. During the...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2018.06.007
更新日期：2018-10-01 00:00:00
abstract：:The three-dimensional structure of the Mcg lambda-type Bence-Jones dimer crystallized in ammonium sulfate is known at 2.3-A resolution. A series of nine other human lambda-chains and two kappa-chains did not crystallize under the same conditions. After these proteins were hybridized with the Mcg light chain by the met...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(85)80001-8
更新日期：1985-02-01 00:00:00
abstract：:The maintenance of intestinal tissue homeostasis is vital for the resistance against inflammatory bowel diseases (IBDs). Necroptosis is identified as an alternative mode of regulated cell death, which plays a pivotal role in tissue homeostasis. Thus, the roles of RIP3-mediated necroptosis in intestinal inflammation ha...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.01.018
更新日期：2019-03-01 00:00:00
abstract：:Excessive production of pro-inflammatory cytokines such as interleukin-1β (IL-1β) plays a key role in the pathophysiological development of osteoarthritis (OA). Orexin A is an important peptide of hypothalamic origin, which has displayed its multiple biological functions in several chronic diseases via activation of i...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2018.05.012
更新日期：2018-09-01 00:00:00
abstract：:Art v 3, the lipid-transfer protein (LTP) of Artemisia vulgaris pollen is a relevant allergen showing frequent cross-reactivity with homologues in other plants. Here we report the identification of four full-length Art v 3 sequences obtained by cDNA cloning using mass spectrometry-based sequencing. Two isoforms, Art v...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.03.021
更新日期：2009-06-01 00:00:00
abstract：:As demonstrated by others, diisopropyl fluorophosphate (DFP) markedly inhibits the O2- generation from guinea-pig polymorphonuclear leukocytes (PMN) stimulated by an antibody complex with ovalbumin (Ag-Ab complex), and also the intracellular uptake of antibody-sensitized erythrocytes by the cells. However, when PMN we...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90113-0
更新日期：1985-09-01 00:00:00
abstract：:C1q, the recognition molecule of the classical pathway of complement, binds to endothelial cells, leading to cell activation. Mannose-binding lectin (MBL), a recognition molecule of the lectin pathway, is structurally and functionally related to C1q. Therefore, we investigated the interaction of MBL with human umbilic...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.06.015
更新日期：2007-02-01 00:00:00
abstract：:Tunicamycin inhibited the synthesis of glycosylated mu-chains and kappa-chains in the human lymphoblastoid cell line, Daudi. Nonglycosylated IgM could not be detected on the surface of tunicamycin-treated cells by cell surface iodination techniques even under conditions where membrane IgM was re-expressed in control c...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90106-2
更新日期：1983-01-01 00:00:00
abstract：:Effective use of adenovirus-5 (Ad5) in cancer therapy is heavily dependent on the degree to which the virus's natural tropism can be subverted to one that favours tumour cells. This is normally achieved through either engineering of the viral fiber knob or the use of bispecific adaptors that display both adenovirus an...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.08.014
更新日期：2015-12-01 00:00:00
abstract：:A technique was developed for identifying peptides with high affinity for a given antibody. By testing a monoclonal antibody directed against a discontinuous antigenic determinant on foot-and-mouth disease virus, peptides mimicking the determinant were identified even though the tertiary structure of the proteins comp...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90081-7
更新日期：1986-07-01 00:00:00
abstract：:An efficient method is described for the isolation of highly purified, IgG-free and stable guinea-pig serum C1q. The procedure includes the chromatography of EDTA-treated serum (25 mM EDTA) on CM- and DEAE-cellulose followed by gel filtration on ACA 34-Ultrogel whereby ammonium sulfate precipitation was used for conce...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90133-5
更新日期：1983-02-01 00:00:00
abstract：:Major histocompatibility complex (MHC) molecules present peptide antigens to T lymphocytes and initiate immune responses. The peptides loaded onto MHC class I or MHC class II molecules can be derived from cytosolic proteins, both self and foreign. A variety of cellular processes, including endocytosis, vesicle traffic...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2018.05.009
更新日期：2018-07-01 00:00:00
abstract：:Autoimmunity is fundamentally a continuously evolving process. The autoimmune responses shift, drift and diversify with time not only to other epitopes in the original antigen but also to other related and sometimes to unrelated antigens. We have described a form of immune diversification--reciprocal T-B epitope sprea...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2003.11.029
更新日期：2004-02-01 00:00:00
abstract：:The mis-regulation of nuclear factor-kappa B (NF-kappaB) signal pathway is involved in a variety of inflammatory diseases that leds to the production of inflammatory mediators. Our studies using human U937 promonocytes cells suggested that magnolol, a low molecular weight lignan isolated from the medicinal plant Magno...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.12.004
更新日期：2007-04-01 00:00:00
abstract：:We have recently developed an in vitro culture model enabling the large-scale expansion of switched-memory B lymphocytes, producing a polyclonal human IgG repertoire. Given the importance of glycosylation for the functions of immunoglobulins, we analyzed the N-glycosylation profiles of the immunoglobulin G (IgG) in th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2013.10.005
更新日期：2014-02-01 00:00:00
