Abstract：
:Tunicamycin inhibited the synthesis of glycosylated mu-chains and kappa-chains in the human lymphoblastoid cell line, Daudi. Nonglycosylated IgM could not be detected on the surface of tunicamycin-treated cells by cell surface iodination techniques even under conditions where membrane IgM was re-expressed in control cultures following the enzymatic stripping of the existing membrane IgM. Biosynthetic labeling and subsequent immunochemical analysis indicated that the nonglycosylated mu and kappa-chains failed to efficiently assemble into monomeric IgM units. In a previous study (Dulis et al., J. biol. Chem., 1982), we have shown that the nonglycosylated mu- and kappa-chains are rapidly catabolized. The lack of expression of nonglycosylated IgM could be due to the rapid catabolism of the nonglycosylated polypeptide chains and/or to the inability to form functional monomeric IgM molecules. Thus glycosylation may be required to protect the newly synthesized polypeptide chains from intracellular catabolic events and to maintain proper conformational foldings of the polypeptide chains to allow for the assembly of subunits into functional units and their ultimate expression.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Kubo RT,Pelanne MLdoi
10.1016/0161-5890(83)90106-2subject
Has Abstractpub_date
1983-01-01 00:00:00pages
67-76issue
1eissn
0161-5890issn
1872-9142journal_volume
20pub_type
杂志文章abstract：:Affinity-purified major histocompatibility complex (MHC) class II molecules are known to bind antigenic peptides in vitro. The percentage of MHC class II molecules occupied with such peptides is usually very low and varies significantly depending upon the sequence and size of a given antigenic peptide. The present stu...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(94)90030-2
更新日期：1994-10-01 00:00:00
abstract：:Serotonin release from rat basophilic leukemia (RBL) cells, sensitized with a DNP-binding monoclonal IgE, was stimulated with solid surface (polystyrene)-bound DNP-amino acids. The stimulatory potency of DNP-amino acids was dependent on the structure of amino acid attached to DNP. Generally, DNP-amino acids with high ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90134-6
更新日期：1991-06-01 00:00:00
abstract：:Invertebrate cells lack the p53 recombination checkpoint but contain mobile DNA sequences that transpose by a mechanism in part shared with excision of the V(D)J recombination signal sequences (RSS). In this work, inversion, deletion, and duplication of sequences associated with an invertebrate C. elegans Tc6 element ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(99)00034-6
更新日期：1999-05-01 00:00:00
abstract：:As an initial step towards the molecular analysis of the murine V kappa locus, a cosmid library from BALB/cJ mouse liver DNA was screened with probes representing 10 V kappa families. Of eight cosmids that were isolated from the initial screen, five contained a single restriction fragment that hybridized to the probes...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90015-p
更新日期：1992-03-01 00:00:00
abstract：:Lysozyme is an important component of the insect non-specific immune response against bacteria that is characterized by its ability to break down bacterial cell-walls. By searching an EST database from the fall armyworm, Spodoptera frugiperda (Negre et al., 2006), we identified five sequences encoding proteins of the ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.09.028
更新日期：2009-12-01 00:00:00
abstract：:Dendritic cells (DC) are comprised of several subsets with distinct functions, differing in their capacity to respond to pathogen products. To gain novel insights into their pathogen specificity, we compared the protein composition of the plasma membrane of CD8+ and CD8- DC, directly isolated from mouse spleens. Diffe...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.02.028
更新日期：2010-05-01 00:00:00
abstract：:The murine pre-B cell line R2-bfl, which can be induced to differentiate in vitro, was used to study germline transcription of variable regions of the light chain loci. RNA from these cells was subjected to a 3'-RACE and germline transcripts from 17 individual Vkappa gene segments belonging to 12 Vkappa families were ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(03)00226-8
更新日期：2003-12-01 00:00:00
abstract：:Human IgE antibodies from nine allergic subjects were found to react with poly-L-lysine (PLL) and other polyamines. Radioimmunoassay inhibition studies indicated that the two amino groups, but not the carboxyl, in lysine contributed to the antibody binding and 4-aminomethyl-1,8-octanediamine, a compound containing thr...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(00)00043-2
更新日期：2000-04-01 00:00:00
abstract：:An efficient method is described for the isolation of highly purified, IgG-free and stable guinea-pig serum C1q. The procedure includes the chromatography of EDTA-treated serum (25 mM EDTA) on CM- and DEAE-cellulose followed by gel filtration on ACA 34-Ultrogel whereby ammonium sulfate precipitation was used for conce...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90133-5
更新日期：1983-02-01 00:00:00
abstract：:The C-terminal domain of protein 4.1G was identified to interact with the cytosolic tail of the high affinity IgG receptor, Fc gamma RI, in yeast two-hybrid screens. Proteins of the 4.1 family have previously been found to mediate receptor/cytoskeleton interactions. In the study presented here, we show an alternativel...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.10.024
更新日期：2008-04-01 00:00:00
abstract：:T-cell-replacing factor (TRF)/IL-5 is a T-cell-derived glycoprotein which has pleiotropic activity on lymphoid and myeloid cells. IL-5 polypeptide translated into Xenopus oocytes are heterogeneous in molecular size (40,000 to 60,000 under nonreducing conditions) and yields a monomeric form (Mr of 25,000 to 30,000) und...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90158-v
更新日期：1990-09-01 00:00:00
abstract：:In this study, a novel approach for the development of a peptide-based vaccine has been tested. We investigated the possibility of replacing an all-L amino acid peptide sequence corresponding to the protective B-cell epitope hemagglutinin (HA) 91-108 from influenza HA with a retro-inverso analogue encompassing this se...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(02)00095-0
更新日期：2002-10-01 00:00:00
abstract：:The paper evaluates the role of the concept of degeneracy in current immunology. The historical background of the notion is considered, as well as that of related concepts like 'cross-reactivity' and 'promiscuity'. A distinction is made between degeneracy, as a property of a system and promiscuity, as a property of an...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2003.11.021
更新日期：2004-02-01 00:00:00
abstract：:Mammary epithelia and the epidermis share a common embryologic origin. Like resident epidermal T cells (ETC), a significant number of murine mammary gland T cells (MTC) express Vgamma5-T-cell receptors (TCR). MTC were assessed to see if they express the same invariant Vgamma5/Vdelta1-TCRs as those of ETC. MTC-derived ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.05.010
更新日期：2006-03-01 00:00:00
abstract：:Ectopic follicles are non-encapsulated organized lymphoid structures that form at sites of inflammation and presumably contribute to the activation and differentiation of cells with autoreactive potential within target tissues. As such, directed targeting of ectopic follicles in settings of autoimmunity may provide a ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.05.006
更新日期：2014-12-01 00:00:00
abstract：:Although the lambda-bearing antibodies represent only 5% of the total mouse serum immunoglobulins, some antigens such as B1355 dextran (alpha (1-3)Dex), the 4-hydroxy-3-nitrophenyl acetyl (NP) and 2,4-dinitro or 2,4,6-trinitrophenyl (DNP/TNP) antigens can induce lambda-positive immune responses. In contrast to the lam...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90061-2
更新日期：1988-02-01 00:00:00
abstract：:The structural and functional integrity of the cell is largely maintained by protein-protein interactions. Recently, we demonstrated that multiple antigenic peptides (MAPs) constructed from 60 kDa Ro sequence could be used to show intramolecular and intermolecular protein-protein interaction within the 60 kDa Ro ribon...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.03.036
更新日期：2004-07-01 00:00:00
abstract：:Human IgE against helminths is a normal component of the whole protective response elicitesd during infection, when specific IgE to a great number of antigens is produced; however, few of those IgE binding components are actually allergens. In general, considering the strong Th2/IgE responses during helminth infection...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2018.03.014
更新日期：2018-08-01 00:00:00
abstract：:The capsid protein of hepatitis B virus (P19) is made of 183 amino acids and carries the antigenic sites of hepatitis B core antigen (HBcAg) and hepatitis B e antigen (HBeAg) on the amino-terminal domain. The carboxyl-terminal domain of P19 (amino acids 150-183) is arginine-rich (47%) and faces the interior of the nuc...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90130-2
更新日期：1989-04-01 00:00:00
abstract：:The T-dependent BALB/c murine immune response to fluorescein (F1) is characterized by structural heterogeneity at the protein level exemplified in part by a significantly wide range of affinities (Ka), and apparent lack of dominant idiotypes. In order to generate an idiotype family, xenogenic anti-idiotype (anti-ID) a...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90072-0
更新日期：1985-08-01 00:00:00
abstract：:Inhibitory antibodies directed against blood coagulation factor VIII (FVIII) impair FVIII replacement therapy, constituting a serious complication in haemophilic and autoimmune patients. Identifying B-cell FVIII epitopes and mapping them on the molecule remain important challenges. Using a combination of different alg...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2006.09.034
更新日期：2007-03-01 00:00:00
abstract：:Glycophorin, one of the major glycoproteins of erythrocytes (E), was extracted from human E (glycophorin-Hu) and guinea pig E (glycophorin-GP) and adsorbed to rabbit-E. The adsorption of glycophorin-Hu and glycophorin-GP to rabbit-E made the E resistant to hemolysis by human serum and guinea pig serum, respectively, v...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90148-7
更新日期：1983-11-01 00:00:00
abstract：:This paper describes additional structural analyses of the p24 cell surface molecule recognized by monoclonal antibody BA-2. Since BA-2 is broadly reactive with a variety of normal and malignant lymphohematopoietic and nonlymphohematopoietic cells, we examined the structure of p24 expressed on different cell types. Tr...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90007-0
更新日期：1985-10-01 00:00:00
abstract：:The Pacific oyster (Crassostrea gigas) is farmed globally. Ostreid herpesvirus (OsHV-1) causes severe mortalities of farmed C. gigas. Management of OsHV-1 has proven difficult. Oysters treated with poly(I:C) exhibit enhanced protection (EP) against OsHV-1. This chemical treatment is highly effective, but it is not fea...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.09.002
更新日期：2016-10-01 00:00:00
abstract：:Interplay between complement factors, regulatory proteins, anaphylatoxins and cytokines could be involved in tendon healing and scar formation. The expression and regulation of complement factors by cytokines or anaphylatoxins are completely unclear in tendon. Hence, the gene expression of the anaphylatoxin receptors ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.09.001
更新日期：2013-04-01 00:00:00
abstract：:Subunits of the mitochondrial ATP synthase complex are expressed on the surface of tumors, bind the TCR of human Vgamma9/Vdelta2 lymphocytes and promote their cytotoxicity. Present experiments show that detection of the complex (called ecto-F1-ATPase) at the cell surface by immunofluorescence correlates with low MHC-c...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.05.026
更新日期：2008-01-01 00:00:00
abstract：:The antigen-limiting nature of microtiter ELISAs predicts that antibodies of minor classes may be underestimated when the same specimen contains large amounts of IgG antibodies specific for the same antigen. Such competitive inhibition can be diagnosed from ELISA titration plots. A method is described to eliminate the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(90)90044-z
更新日期：1990-04-01 00:00:00
abstract：:The effect of fragment B of staphylococcal protein A on the binding of rabbit IgG to human granulocytes has been examined. When rabbit IgG sensitized sheep erythrocytes (EA) were preincubated with increasing amounts of fragment B, a dose dependent inhibition of rosette formation between the human granulocytes and the ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90206-1
更新日期：1982-03-01 00:00:00
abstract：:Signal transduction by the B cell receptor (BCR) is an absolute requirement for the selection and development of B lymphocytes at multiple checkpoints. Binding to antigen via the BCR is complemented by a co-stimulus delivered through accessory and co-stimulatory cell surface molecules that regulate the signalling thre...
journal_title：Molecular immunology
pub_type：
doi：10.1016/s0161-5890(02)00203-1
更新日期：2003-01-01 00:00:00
abstract：:During the last years, intensive research has shed light in the transcriptional networks that shape the invariant NKT (iNKT) cell lineage and guide the choices towards functionally distinct iNKT cell subsets (Constantinides and Bendelac, 2013; Engel and Kronenberg, 2014; Gapin, 2016; Kim et al., 2015). However, the ep...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2018.08.020
更新日期：2018-09-01 00:00:00
