Abstract:
:The antigen-limiting nature of microtiter ELISAs predicts that antibodies of minor classes may be underestimated when the same specimen contains large amounts of IgG antibodies specific for the same antigen. Such competitive inhibition can be diagnosed from ELISA titration plots. A method is described to eliminate the negative effects of this competition on the detection of IgA antibodies in rabbit serum. The detectability of rabbit serum antibodies to ovalbumin and bovine serum albumin is increased 10-fold by prior treatment of 1:100 dilutions of serum with 1% Cowan I S. aureus. High concns of S. aureus, e.g. 10%, completely deplete serum IgG without loss of IgA. However, concns higher than 1% do not lead to additional improvement in the detectability of IgA antibodies in the systems studied. The method is rapid, inexpensive and shows no non-specific depletion of IgA from either serum or bronchoalveolar lavage fluid.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Butler JE,Heo Y,Adams P,Richerson HBdoi
10.1016/0161-5890(90)90044-zsubject
Has Abstractpub_date
1990-04-01 00:00:00pages
319-25issue
4eissn
0161-5890issn
1872-9142journal_volume
27pub_type
杂志文章abstract::Extracts of blood lymphocytes, polymorphonuclear neutrophils and B, T or monocytic cell lines were analyzed by two-dimensional gel electrophoresis and immunoradiometric assay after electro-transfer to nitrocellulose sheets with radiolabelled polyclonal or monoclonal antibodies specific for beta 2-microglobulin. Four d...
journal_title:Molecular immunology
pub_type: 杂志文章
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.05.013
更新日期:2004-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.12.013
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journal_title:Molecular immunology
pub_type: 杂志文章
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.08.009
更新日期:2010-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.07.014
更新日期:2016-09-01 00:00:00
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journal_title:Molecular immunology
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journal_title:Molecular immunology
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.09.001
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journal_title:Molecular immunology
pub_type: 杂志文章
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journal_title:Molecular immunology
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pub_type: 杂志文章
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