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Charge heterogeneity of beta 2-microglobulin in lymphoid cells.

Abstract：

:Extracts of blood lymphocytes, polymorphonuclear neutrophils and B, T or monocytic cell lines were analyzed by two-dimensional gel electrophoresis and immunoradiometric assay after electro-transfer to nitrocellulose sheets with radiolabelled polyclonal or monoclonal antibodies specific for beta 2-microglobulin. Four different forms of the molecule were identified with an apparent Mr of 12,000 and pI values of 5.7, 5.3 and lower. Lymphocyte activation by phytohemagglutinin and concanavalin A, or incubation with recombinant alpha 2b interferon, resulted in an increased beta 2-microglobulin cell content and release of the protein in supernatants with a predominant elevation of the more acidic minor forms. Recombinant interleukin-2 and recombinant gamma interferon increased the expression of the molecule without significant shift in the relative proportion of beta 2-microglobulin forms. Tumor necrosis factor alpha did not increase cell beta 2-microglobulin (beta 2-m) content and release and did not alter the relative distribution of the different forms of the molecule. Several mechanisms may be considered for the generation of beta 2-m microheterogeneity, including intracytoplasmic post-translational modifications such as proteolysis or modification of the amide groups of internal amino acids.

journal_name

Mol Immunol

journal_title

Molecular immunology

authors

Vincent C,Ramackers JM,Bonnefoy N,Revillard JP

doi

10.1016/0161-5890(89)90032-1

subject

Has Abstract

pub_date

1989-08-01 00:00:00

pages

727-33

issue

8

eissn

0161-5890

issn

1872-9142

journal_volume

26

pub_type

杂志文章

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