Cloning, expression analysis and functional characterization of an interleukin-1 receptor-associated kinase 4 from Apostichopus japonicus.


:Interleukin 1 receptor-associated kinase 4 (IRAK4) is a key factor in TLR-mediated host immune function. In this study, an IRAK4 homologue molecule was identified from Apostichopus japonicus (designated as AjIRAK4) by RACE approach. The full-length cDNA of AjIRAK4 was of 2024 bp containing an open reading frame of 1311 bp encoding a 436-amino-acid (aa) residue polyprotein with the typical death domain (10-113aa) and the kinase domain (160-426aa). The mRNA transcripts of AjIRAK4 displayed constitutively expressed in all examined tissues with highest expression in the muscles (7.20-fold increase compared to the coelomocytes). The pathogen Vibrio splendidus challenge and LPS exposure could both significantly up-regulate the mRNA expression of AjIRAK4. Silencing AjIRAK4 in vitro and in vivo could inhibit the expression of TLR members at mRNA and protein levels, suggesting AjIRAK4 was an important component of TLR cascade in sea cucumber. More importantly, knockdown of AjIRAK4 by specific siRNA resulted in the significant promotion of coelomocyte apoptosis by 1.82-fold increase in vitro and 1.95-fold in vivo. Taken together, all our results suggested that AjIRAK4 might be served as coelomocyte apoptosis regulator via TLR cascade.


Mol Immunol


Molecular immunology


Cui Y,Jiang L,Xing R,Wang Z,Wang Z,Shao Y,Zhang W,Zhao X,Li C




Has Abstract


2018-09-01 00:00:00












  • The autoimmune TCR-Ob.2F3 can bind to MBP85-99/HLA-DR2 having an unconventional mode as in TCR-Ob.1A12.

    abstract::The generation of T cell receptor (TCR) sequence diversity can produce 'forbidden' clones able to recognize self-antigens. Here, the structure of the complex between a myelin basic protein peptide (MBP85-99), human leukocyte antigen (HLA)-DR2 (DRB1*1501/DRA) and TCR-Ob.2F3, the dominant autoimmune clone obtained from ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Kato Z,Stern JN,Nakamura HK,Miyashita N,Kuwata K,Kondo N,Strominger JL

    更新日期:2010-11-01 00:00:00

  • RIP2-beta: a novel alternative mRNA splice variant of the receptor interacting protein kinase RIP2.

    abstract::RIP2/RICK/CARDIAK is a member of the receptor interacting protein kinase (RIP) family. RIP2 promotes NF-kappaB activation as well as activation of the MAPKs JNK, ERK1/2 and p38 MAPK, thereby playing an emergent role in the innate immune response and NOD signaling. Moreover, RIP2 has been shown to interact with the CAR...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Krieg A,Le Negrate G,Reed JC

    更新日期:2009-03-01 00:00:00

  • Calcium signaling in B cells: regulation of cytosolic Ca2+ increase and its sensor molecules, STIM1 and STIM2.

    abstract::Calcium signals are crucial for diverse cellular functions including adhesion, differentiation, proliferation, effector functions and gene expression. After engagement of the B cell receptor, the intracellular calcium ion (Ca(2+)) concentration is increased promoting the activation of various signaling cascades. While...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Baba Y,Matsumoto M,Kurosaki T

    更新日期:2014-12-01 00:00:00

  • Cathepsin S inhibition changes regulatory T-cell activity in regulating bladder cancer and immune cell proliferation and apoptosis.

    abstract::Regulatory T cells (Tregs) are immune suppressive cells, but their roles in tumor growth have been elusive, depending on tumor type or site. Our prior study demonstrated a role of cathepsin S (CatS) in reducing Treg immunosuppressive activity. Therefore, CatS inhibition in Tregs may exacerbate tumor growth. Using mous...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Yan X,Wu C,Chen T,Santos MM,Liu CL,Yang C,Zhang L,Ren J,Liao S,Guo H,Sukhova GK,Shi GP

    更新日期:2017-02-01 00:00:00

  • Non-enzymic activation of the fifth component of human complement, by oxygen radicals. Some properties of the activation product, C5b-like C5.

    abstract::Purified human C5 was converted non-enzymically to an activated form as defined by its ability to participate in reactive lysis. This conversion occurred following exposure to systems that generate oxygen radicals, namely addition of H2O2 in the presence of ascorbic acid and iron or the addition of xanthine oxidase, a...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Vogt W,Damerau B,von Zabern I,Nolte R,Brunahl D

    更新日期:1989-12-01 00:00:00

  • Identification of chicken cathelicidin-2 core elements involved in antibacterial and immunomodulatory activities.

    abstract::Chicken host defense peptide cathelicidin-2 (CATH-2) is known to exert antimicrobial and immunomodulatory activities and consists of two alpha-helices connected by a hinge region. Here we report the biological properties of the separate alpha-helical segments and the importance of the proline residue in the hinge regi...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: van Dijk A,Molhoek EM,Veldhuizen EJ,Bokhoven JL,Wagendorp E,Bikker F,Haagsman HP

    更新日期:2009-08-01 00:00:00

  • Of mice and men: The factor H protein family and complement regulation.

    abstract::For decades immunological research has relied, with variable success, on mouse models to investigate diseases and possible therapeutic interventions. With the approval of the first therapeutic antibody targeting complement, called eculizumab, as therapy in paroxysmal nocturnal hemoglobinuria (PNH) and more recently at...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Pouw RB,Vredevoogd DW,Kuijpers TW,Wouters D

    更新日期:2015-09-01 00:00:00

  • In vitro activation of murine peritoneal macrophages by ultraviolet B radiation: upregulation of CD18, production of NO, proinflammatory cytokines and a signal transduction pathway.

    abstract::In the present study, we report the activation of murine peritoneal macrophages in vitro on irradiation with sublethal dose of UVB (50 mJ/cm(2)). The activation involves enhanced expression of CD18 molecule and production of nitric oxide (NO), tumor necrosis factor (TNF-alpha) and interleukin-1 (IL-1). Production of N...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Sethi G,Sodhi A

    更新日期:2004-04-01 00:00:00

  • C1q binding by a high affinity anti-fluorescein murine monoclonal IgM antibody and monomeric subunits.

    abstract::Comparative interactions of purified rabbit C1q with 18-2-3, a high affinity (2-3 X 10(10) M-1) anti-fluorescein (anti-F1) murine monoclonal IgM antibody (pentamer) and constitutive monomeric subunits (IgMs) were studied. Using a solid phase radioimmunoassay (SPRIA), based on immobilized polyvalent antigen, it was sho...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Swanson SM,Dombrink-Kurtzman MA,Voss EW Jr

    更新日期:1988-06-01 00:00:00

  • A male-specific expression gene, encodes a novel anionic antimicrobial peptide, scygonadin, in Scylla serrata.

    abstract::Scygonadin is a novel antimicrobial peptide, which was originally isolated from the seminal plasma of the mud crab, Scylla serrata. Based on the partial 20-residue NH(2)-terminal sequence of the peptide, H-Gly-Gln-Ala-Leu-Asn-Lys-Leu-Met-Pro-Lys-Ile-Val-Ser-Ala-Ile-Ile-Tyr-Met-Val-Gly-OH, scygonadin was cloned from th...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Wang KJ,Huang WS,Yang M,Chen HY,Bo J,Li SJ,Wang GZ

    更新日期:2007-03-01 00:00:00

  • Structural comparison of murine T-cell (B151K12)-derived T-cell-replacing factor (IL-5) with rIL-5: dimer formation is essential for the expression of biological activity.

    abstract::T-cell-replacing factor (TRF)/IL-5 is a T-cell-derived glycoprotein which has pleiotropic activity on lymphoid and myeloid cells. IL-5 polypeptide translated into Xenopus oocytes are heterogeneous in molecular size (40,000 to 60,000 under nonreducing conditions) and yields a monomeric form (Mr of 25,000 to 30,000) und...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Takahashi T,Yamaguchi N,Mita S,Yamaguchi Y,Suda T,Tominaga A,Kikuchi Y,Miura Y,Takatsu K

    更新日期:1990-09-01 00:00:00

  • An in vitro model of T cell receptor revision in mature human CD8+ T cells.

    abstract::V(D)J recombination is a mechanism peculiar to the somatic rearrangement of antigen receptor genes. It requires both expression of the RAG-1 and RAG-2 recombinases and accessibility of the substrate to its recombinase and post-cleavage/DNA repair stage. TCR revision is a genetic correction mechanism that changes T cel...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Lantelme E,Orlando L,Porcedda P,Turinetto V,De Marchi M,Amoroso A,Mantovani S,Giachino C

    更新日期:2008-01-01 00:00:00

  • Biological and molecular docking studies on coagulin-H: Human IL-2 novel natural inhibitor.

    abstract::Withanolide, coagulin-H (1), was evaluated for its effect on various cellular functions related to immune response including lymphocyte proliferation, and expression of interleukin-2 (IL-2) cytokine, and results were compared with prednisolone (2), a commonly used immune modulating drug. Coagulin-H (1) was found to ha...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Mesaik MA,Zaheer-Ul-Haq,Murad S,Ismail Z,Abdullah NR,Gill HK,Atta-Ur-Rahman,Yousaf M,Siddiqui RA,Ahmad A,Choudhary MI

    更新日期:2006-04-01 00:00:00

  • Blocking oncogenic RAS enhances tumour cell surface MHC class I expression but does not alter susceptibility to cytotoxic lymphocytes.

    abstract::Mutations in the RAS family of oncogenes are highly prevalent in human cancer and, amongst its manifold effects, oncogenic RAS impairs the expression of components of the antigen presentation pathway. This allows evasion of cytotoxic T lymphocytes (CTL). CTL and natural killer (NK) cells are reciprocally regulated by ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: El-Jawhari JJ,El-Sherbiny YM,Scott GB,Morgan RS,Prestwich R,Bowles PA,Blair GE,Tanaka T,Rabbitts TH,Meade JL,Cook GP

    更新日期:2014-04-01 00:00:00

  • TCR transfer induces TCR-mediated tonic inhibition of RAG genes in human T cells.

    abstract::Induction of the TCR signaling pathway terminates the expression of RAG genes, and a link between this pathway and their transcriptional control is evident from the recent demonstration of their re-expression if the TCR is subsequently lost or down-regulated. Since unstimulated T cells display a steady-state level of ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Orlando L,Accomasso L,Circosta P,Turinetto V,Lantelme E,Porcedda P,Minieri V,Pautasso M,Willemsen RA,Cignetti A,Giachino C

    更新日期:2011-07-01 00:00:00

  • Evolutionary implication of B-1 lineage cells from innate to adaptive immunity.

    abstract::The paradigm that B cells mainly play a central role in adaptive immunity may have to be reevaluated because B-1 lineage cells have been found to exhibit innate-like functions, such as phagocytic and bactericidal activities. Therefore, the evolutionary connection of B-1 lineage cells between innate and adaptive immuni...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Zhu LY,Shao T,Nie L,Zhu LY,Xiang LX,Shao JZ

    更新日期:2016-01-01 00:00:00

  • Inflammatory pathways in spondyloarthritis.

    abstract::Spondyloarthritis is the second most common form of chronic inflammatory arthritis and a unique hallmark of the disease is pathologic new bone formation. Several cytokine pathways have been genetically associated with ankylosing spondylitis (AS), the prototypic subtype of SpA, and additional evidence from human and an...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Hreggvidsdottir HS,Noordenbos T,Baeten DL

    更新日期:2014-01-01 00:00:00

  • Complement: a unique innate immune sensor for danger signals.

    abstract::The complement (C) inflammatory cascade is part of the phylogenetically ancient innate immune response and is crucial to our natural ability to ward off infection. It has three critical physiologic activities: (i) defending against microbial infections by triggering the generation of a membranolytic complex (C5b9 comp...

    journal_title:Molecular immunology

    pub_type: 杂志文章,评审


    authors: Gasque P

    更新日期:2004-11-01 00:00:00

  • IL-37 induces autophagy in hepatocellular carcinoma cells by inhibiting the PI3K/AKT/mTOR pathway.

    abstract::Autophagy is an intracellular "self-eating" process that is closely related to inflammation and cellular immunity. New studies indicate that autophagy is also involved in tumor suppression. The anti-inflammatory cytokine interleukin-37 (IL-37) has been shown to have tumor-suppressive abilities in hepatocellular carcin...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Li TT,Zhu D,Mou T,Guo Z,Pu JL,Chen QS,Wei XF,Wu ZJ

    更新日期:2017-07-01 00:00:00

  • Isolation and sequence of a cDNA coding for the immunoglobulin mu chain of the sheep.

    abstract::A sheep cDNA library was screened with a human C mu probe, and the complete nucleotide sequence of a 1923 nt cDNA was determined. It contains sequences corresponding to all the exons (VH, DH, JH, CH1, CH2, CH3 and CH4) characteristic of the immunoglobulin mu heavy chain regions. The deduced amino acid sequence shows a...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Patri S,Nau F

    更新日期:1992-07-01 00:00:00

  • Characterisation of Concanavalin A-binding glycoproteins from mouse splenic leukocytes by two-dimensional electrophoresis: preferential binding of incompletely glycosylated forms of H-2 antigen to the lectin.

    abstract::Concanavalin A (Con A)section-binding proteins from mouse spleen leukocytes were characterised by two-dimensional electrophoresis of material precipitated, by Con A plus anti-Con A, from lysates of biosynthetically-labelled cells. Although most cell surface (iodinatable) proteins are known to bind Con A, some of the m...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Pink JR,Hoessli D,Tartakoff A,Hooghe R

    更新日期:1983-04-01 00:00:00

  • Molecular cloning and characterization of CD9 cDNA from cartilaginous fish, red stingray, Dasyatis akajei.

    abstract::CD9 is a glycoprotein of the transmembrane 4 superfamily (TM4SF) and is involved in various cellular processes. In this study, we describe the isolation of the full-length cDNA encoding for CD9 molecule (daCD9) of red stingray, Dasyatis akajei. This 1252 bp cDNA was isolated from leukocyte cDNA library and contains 68...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Zhu J,Yan K,Lu L,Peng C,Zhou C,Chen S,Xie X,Dong M,Xu A

    更新日期:2006-04-01 00:00:00

  • Mechanism of allergic cross-reactions--II. Cross-stimulation, by chemically unrelated ligands, of rat basophilic leukemia cells sensitized with an anti-DNP IgE antibody.

    abstract::Serotonin release from rat basophilic leukemia (RBL) cells, sensitized with a DNP-binding monoclonal IgE, was stimulated with solid surface (polystyrene)-bound DNP-amino acids. The stimulatory potency of DNP-amino acids was dependent on the structure of amino acid attached to DNP. Generally, DNP-amino acids with high ...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Varga JM,Kalchschmid G,Klein GF,Fritsch P

    更新日期:1991-06-01 00:00:00

  • Molecular comparison of mouse Thy-1 and its human homologue.

    abstract::Physicochemical techniques were used to verify previous immunochemical studies showing homology of the human Thy-1 (formerly p25) antigen and the murine Thy-1 or theta antigen. Peptide maps and amino acid compositions showed close similarity between these two proteins; however, they were not identical. These data conf...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Bonewald L,Wang AC,Terry E,Zwerner R,Balch CM,Ades EW

    更新日期:1982-03-01 00:00:00

  • Pre-existing CD19-independent GL7(-) Breg cells are expanded during inflammation and in mice with lupus-like disease.

    abstract::Interleukin 10 (IL-10)-producing regulatory B-cells (Bregs) suppress inflammatory responses that mediate autoimmune diseases. However, it is unknown whether Bregs derive from a pre-existing dedicated B-cell lineage or if any B-cell can differentiate into Bregs in response to BCR or TLR activation. GL7(+) B-cells are a...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Wang X,Wei Y,Xiao H,Liu X,Zhang Y,Han G,Chen G,Hou C,Zhang L,Ma N,Shen B,Li Y,Egwuagu CE,Wang R

    更新日期:2016-03-01 00:00:00

  • PVR (CD155) and Nectin-2 (CD112) as ligands of the human DNAM-1 (CD226) activating receptor: involvement in tumor cell lysis.

    abstract::The capability of NK lymphocytes to kill tumor cells depends on different receptors/ligands interactions. In order to identify the cellular ligands recognized by "orphan" triggering receptors, mice were immunized with NK susceptible target cells. mAbs were selected that inhibited NK cytotoxicity and recognized two dif...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Pende D,Bottino C,Castriconi R,Cantoni C,Marcenaro S,Rivera P,Spaggiari GM,Dondero A,Carnemolla B,Reymond N,Mingari MC,Lopez M,Moretta L,Moretta A

    更新日期:2005-02-01 00:00:00

  • Engineering the binding properties of the T cell receptor:peptide:MHC ternary complex that governs T cell activity.

    abstract::The potency of a T cell is determined in large part by two interactions, binding of a cognate peptide to the MHC, and binding of the T cell receptor (TCR) to this pepMHC. Various studies have attempted to assess the relative importance of these interactions, and to correlate the corresponding binding parameters with t...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Bowerman NA,Crofts TS,Chlewicki L,Do P,Baker BM,Christopher Garcia K,Kranz DM

    更新日期:2009-09-01 00:00:00

  • Dysregulation of T cell immunoglobulin and mucin domain 3 (TIM-3) signaling in peripheral immune cells is associated with immune dysfunction in autistic children.

    abstract::Evidence suggests that immune dysregulation is associated with autism spectrum disorder (ASD). T cell immunoglobulin and mucin domain-3 (TIM-3) has a critical role in several inflammatory disorders; however, the role of TIM-3 signaling has not been demonstrated in ASD. In the present study, we assessed the role of TIM...

    journal_title:Molecular immunology

    pub_type: 临床试验,杂志文章


    authors: Ahmad SF,Ansari MA,Nadeem A,Bakheet SA,Al-Ayadhi LY,Alotaibi MR,Alhoshani AR,Alshammari MA,Attia SM

    更新日期:2019-02-01 00:00:00

  • Identification of MHC class IIbeta resistance/susceptibility alleles to Aeromonas salmonicida in brook charr (Salvelinus fontinalis).

    abstract::Pathogen-driven selection is believed to be important in the evolution and maintenance of the polymorphism of the major histocompatibility complex (MHC) genes but have been tested for very few vertebrates. In this study, we first investigate by SSCP (single strand conformational polymorphism) the diversity found at th...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Croisetière S,Tarte PD,Bernatchez L,Belhumeur P

    更新日期:2008-06-01 00:00:00

  • The role of the light chain in the structure and binding activity of two cattle antibodies that neutralize bovine respiratory syncytial virus.

    abstract::Cattle antibodies have unusually long CDR3 loops in their heavy chains (HCs), and limited light chain (LC) diversity, raising the question of whether these mask the effect of LC variation on antigen recognition. We have investigated the role of the LC in the structure and activity of two neutralizing cattle antibodies...

    journal_title:Molecular immunology

    pub_type: 杂志文章


    authors: Ren J,Nettleship JE,Harris G,Mwangi W,Rhaman N,Grant C,Kotecha A,Fry E,Charleston B,Stuart DI,Hammond J,Owens RJ

    更新日期:2019-08-01 00:00:00