Abstract：
:Interleukin 1 receptor-associated kinase 4 (IRAK4) is a key factor in TLR-mediated host immune function. In this study, an IRAK4 homologue molecule was identified from Apostichopus japonicus (designated as AjIRAK4) by RACE approach. The full-length cDNA of AjIRAK4 was of 2024 bp containing an open reading frame of 1311 bp encoding a 436-amino-acid (aa) residue polyprotein with the typical death domain (10-113aa) and the kinase domain (160-426aa). The mRNA transcripts of AjIRAK4 displayed constitutively expressed in all examined tissues with highest expression in the muscles (7.20-fold increase compared to the coelomocytes). The pathogen Vibrio splendidus challenge and LPS exposure could both significantly up-regulate the mRNA expression of AjIRAK4. Silencing AjIRAK4 in vitro and in vivo could inhibit the expression of TLR members at mRNA and protein levels, suggesting AjIRAK4 was an important component of TLR cascade in sea cucumber. More importantly, knockdown of AjIRAK4 by specific siRNA resulted in the significant promotion of coelomocyte apoptosis by 1.82-fold increase in vitro and 1.95-fold in vivo. Taken together, all our results suggested that AjIRAK4 might be served as coelomocyte apoptosis regulator via TLR cascade.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Cui Y,Jiang L,Xing R,Wang Z,Wang Z,Shao Y,Zhang W,Zhao X,Li Cdoi
10.1016/j.molimm.2018.08.006subject
Has Abstractpub_date
2018-09-01 00:00:00pages
479-487eissn
0161-5890issn
1872-9142pii
S0161-5890(18)30467-Xjournal_volume
101pub_type
杂志文章abstract：:The p38 mitogen-activated protein kinases (MAPKs) are essential cytoplasmic signal molecules of innate immune pathways that play a vital role in host immune defense responses to pathogenic challenges. In this study, two fish p38 genes (Cip38α and Cip38β) were characterized for the first time from the grass carp Ctenop...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.12.007
更新日期：2020-02-01 00:00:00
abstract：:Toll-like receptors (TLRs) are a family of highly conserved germline-encoded pattern-recognition receptors (PRRs) and are essential for host immune response. Little is known regarding the activation mechanism of TLRs especially of the TLR7/8/9 subfamily. Here we cloned and characterized bovine TLR8 (bTLR8) and found t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2008.09.024
更新日期：2009-02-01 00:00:00
abstract：:Glucocorticoids down regulate the inducible nitric oxide synthase (iNOS) gene both transcriptionally and post-transcriptionally. The post-transcriptional events are suggested to involve destabilization of the iNOS transcript although the molecular mechanisms for this effect are not known. Recently, our laboratory demo...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.01.029
更新日期：2007-05-01 00:00:00
abstract：:Peptides representing the amino-terminal sequence of protamines HP1 (sequence 1-12) and HP2 (sequence 1-11), the two major nuclear proteins of human sperm, have been synthesized. Rabbits were immunized either with peptide conjugated with a carrier or with free peptide. The resulting antisera were examined for their ca...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90179-n
更新日期：1991-09-01 00:00:00
abstract：:MicroRNAs (miRNAs) are now recognized as important regulators of all cellular processes, including immune function and cancer survival. These evolutionary preserved, single-stranded, non-coding RNA molecules mediate important functional effects primarily through post-transcriptional regulation of protein expression. M...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2018.07.022
更新日期：2019-11-01 00:00:00
abstract：:Mycobacterium leprae, the causative agent of leprosy, challenges host defense mechanism by impairing the signal transduction of T cells which leads to downregulation of T cell proliferation, mainly as a consequence of interference with IL-2 production. In this study we sought to identify how soluble forms of M. leprae...
journal_title：Molecular immunology
pub_type： 临床试验,杂志文章
doi：10.1016/j.molimm.2006.09.008
更新日期：2007-03-01 00:00:00
abstract：:Monoclonal antibodies (MAbs) raised against trout immunoglobulin (Ig) recognize two independent Ig populations differing in the molecular weight of L chains. The trout Ig population recognized by MAb 2H9 represented congruent to 20% of the total Ig and contained L chains congruent to 26 kDa, whereas the trout Ig popul...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90014-b
更新日期：1991-11-01 00:00:00
abstract：:The innate immune system is the first line of defense against infection and responses are initiated by pattern recognition receptors (PRRs) that detect pathogen-associated molecular patterns (PAMPs). PRRs also detect endogenous danger-associated molecular patterns (DAMPs) that are released by damaged or dying cells. T...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2017.02.012
更新日期：2017-06-01 00:00:00
abstract：:Candida albicans is an opportunistic human-pathogenic fungus, which can cause superficial but also life-threatening invasive infections. The pH-regulated antigen 1 (Pra1) of C. albicans is a surface-associated and secreted protein highly expressed in the hyphal form. Pra1 can bind to complement receptor 3 (CD11b/CD18)...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.07.007
更新日期：2011-09-01 00:00:00
abstract：BACKGROUND:Individuals genetically deficient of properdin are more susceptible to meningococcal disease. Likewise low concentration or decreased biological activity of mannose-binding lectin (MBL) is associated with higher incidence of bacterial infections during childhood. In this study we report our findings in a Dan...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.02.017
更新日期：2006-02-01 00:00:00
abstract：:Dendritic cells (DC) survey epithelial or mucosal surfaces for antigens, take them up via their endocytic or phagocytic receptors, process the captured antigens and migrate to the lymphatic tissues. In the draining lymph nodes they present the immunogenic peptides to T cells thereby inducing antigen-specific immune re...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2004.06.019
更新日期：2005-02-01 00:00:00
abstract：:Effective use of adenovirus-5 (Ad5) in cancer therapy is heavily dependent on the degree to which the virus's natural tropism can be subverted to one that favours tumour cells. This is normally achieved through either engineering of the viral fiber knob or the use of bispecific adaptors that display both adenovirus an...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.08.014
更新日期：2015-12-01 00:00:00
abstract：:Trichosanthin (TCS) is a type I ribosome-inactivating protein with wide spectrum of pharmacological activities. It inhibits human immunodeficiency virus type 1 (HIV-1) and Herpes simplex virus type 1 (HSV-1) replication but the mechanism is not clear. From a previous study, TCS was found to be more cytotoxic to HIV-1 ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2011.06.217
更新日期：2011-09-01 00:00:00
abstract：:NKG2D receptor-ligand interaction triggers NK cell-mediated cytolysis and IFN-gamma secretion. IFN-gamma produced by NK cells has been found to promote the interaction between NK cells and monocytes; however, the underlying mechanism remains elusive. We demonstrate here that IFN-gamma exclusively induced or upregulate...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.10.007
更新日期：2008-03-01 00:00:00
abstract：:The characterization of the site on the IgE molecule which accommodates the high affinity receptor for IgE (Fc epsilon RI) should allow the design of IgE analogues which can be utilized to block allergic responses. Using chimeric human IgE molecules in which different constant region domains were exchanged with their ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90038-y
更新日期：1992-09-01 00:00:00
abstract：:The S100A12 gene belongs to the S100 family of genes, which are specific to vertebrates. It is involved in many inflammatory diseases of human and has been considered as a powerful diagnostic gene. In the present study, we identified the porcine S100A12 (pS100A12) gene, provided evidence that pS100A12 is located on ch...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.01.024
更新日期：2010-04-01 00:00:00
abstract：:Natural killer cells fail to lyse target cells expressing sufficient levels of self MHC class I molecules, providing one mechanism to secure self tolerance. Inhibition of lysis is mediated by inhibitory receptors expressed by NK cells, such as the murine Ly49 receptors, human KIR receptors and CD94/NKG2A, expressed by...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.07.018
更新日期：2005-02-01 00:00:00
abstract：BACKGROUND:Dendritic cells (DCs) and regulatory T (Treg) cells are crucial for inducing immune tolerance. However, the suppressive function of infused Treg cells and immature DCs (imDCs) following solid organ transplantation remains unclear. METHODS:ImDCs derived from DA-donor rats and Treg cells isolated from spleens...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2016.10.004
更新日期：2016-11-01 00:00:00
abstract：:Ectopic follicles are non-encapsulated organized lymphoid structures that form at sites of inflammation and presumably contribute to the activation and differentiation of cells with autoreactive potential within target tissues. As such, directed targeting of ectopic follicles in settings of autoimmunity may provide a ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.05.006
更新日期：2014-12-01 00:00:00
abstract：:The mechanism by which heparin inhibits the alternative complement pathway (ACP) by a fluid-phase activator, CoVF, has been studied. Results presented here indicate that heparin's major (rate-limiting) effect on the fluid-phase activation of the ACP was to potentiate Factor H activity. Such an effect results in a very...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90139-6
更新日期：1983-11-01 00:00:00
abstract：:Multilamellar lipid vesicles (MLV) composed of egg yolk lecithin (EYL) suppressed the response of bovine peripheral blood lymphocytes (BPBL) to phytohemagglutinin (PHA). EYL contains 18:1 and 18:2 as the major unsaturated phospholipids. Dioleoyllecithin (DOL; cis 9) MLV did not suppress BPBL blastogenesis. Dilinoeylle...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90195-x
更新日期：1982-11-01 00:00:00
abstract：:A transplanted organ suffers inherently from an ischaemic insult and subsequent reperfusion injury. The severity of such early events is thought to influence the success of the transplant procedure, not only in the immediate post-transplant period, but also to predispose the graft to both acute and chronic rejection. ...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(99)00118-2
更新日期：1999-09-01 00:00:00
abstract：:Mammary epithelia and the epidermis share a common embryologic origin. Like resident epidermal T cells (ETC), a significant number of murine mammary gland T cells (MTC) express Vgamma5-T-cell receptors (TCR). MTC were assessed to see if they express the same invariant Vgamma5/Vdelta1-TCRs as those of ETC. MTC-derived ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2005.05.010
更新日期：2006-03-01 00:00:00
abstract：BACKGROUND:A T cell subtype with the CD4+CD25-FoxP3+ phenotype was recently described. We aimed to investigate the frequency of these cells and their ability to produce cytokines in tumor-draining lymph nodes from patients with breast cancer (BC). MATERIALS AND METHODS:Mononuclear cells from lymph nodes of 20 patients...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.10.007
更新日期：2019-12-01 00:00:00
abstract：:Interplay between complement factors, regulatory proteins, anaphylatoxins and cytokines could be involved in tendon healing and scar formation. The expression and regulation of complement factors by cytokines or anaphylatoxins are completely unclear in tendon. Hence, the gene expression of the anaphylatoxin receptors ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2012.09.001
更新日期：2013-04-01 00:00:00
abstract：:Immature B cell lines transformed with a temperature-sensitive mutant of Abelson murine leukaemia virus undergo preferentially V gamma 2 to J gamma 2 joinings during culture at a non-permissive temperature (39 degrees C). Here we examined nucleotide addition and deletion at the V gamma 2-J gamma 2 junctions in these V...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(92)90123-f
更新日期：1992-07-01 00:00:00
abstract：:This study was undertaken to investigate the nature and microheterogeneity of the carbohydrate moiety of the Fc epsilon receptors of RBL-CA10 and RBL-CA10.7 cells. Treatment using the glycosylation processing inhibitors, castanospermine (CN), 1-deoxymannojirimycin (DMJ), and swainsonine (SW) resulted in a decrease of ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(93)90060-o
更新日期：1993-02-01 00:00:00
abstract：:Syngeneic polyclonal antibodies were elicited to an affinity labeled high affinity (2-3 X 10(10) M-1) anti-fluorescein murine IgG2a monoclonal antibody. Hyperimmune ascites fluid was tested for reactivity with homologous liganded, affinity labeled and non-liganded Fab fragments derived from the high affinity antibody....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90111-3
更新日期：1988-08-01 00:00:00
abstract：:To date, although a promising anti-inflammatory activity of oligomeric proanthocyanidins (OPCs) has been observed in asthma, the mechanism responsible for these immunomodulatory properties remains obscure. Dendritic cells (DCs) that reside in the airway have been widely perceived as an important contributor to asthma....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.09.012
更新日期：2017-11-01 00:00:00
abstract：:The complement system plays a central role in a number of human inflammatory diseases, and there is a significant need for development of complement-directed therapies. The discovery of an arsenal of anti-complement proteins secreted by the pathogen Staphylococcus aureus brought with it the potential for harnessing th...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.05.012
更新日期：2015-10-01 00:00:00
