Abstract：
:Intercellular adhesion molecule-1 (ICAM-1) functions in leukocyte trafficking, activation, and the formation of the immunological synapse. ICAM-1 is a member of the immunoglobulin superfamily of adhesion proteins, which share a similar structure of repeating Ig-like domains. Many genes in this family, including ICAM-1, show alternative splicing leading to the production of different protein isoforms, although little functional information is available regarding the expression patterns, ligand interactions, and functions of these isoforms, especially those arising from the ICAM-1 gene. In this study, we show using different lines of mutant mice (Icam1(tm1Jcgr) and Icam1(tm1Bay)) that alterations in the expression of the alternatively spliced ICAM-1 isoforms can significantly influence the disease course during the development of EAE. Icam1(tm1Jcgr) mutant mice, unlike Icam1(tm1Bay) mutants, do not express isoforms containing the Mac-1 binding domain and had significantly attenuated of EAE. In contrast, Icam1(tm1Bay) mice developed severe EAE in both active and adoptive transfer models compared to both Icam1(tm1Jcgr) and wild type mice. We also observed that T cells from Icam1(tm1Bay) mice displayed increased proliferation kinetics and produced higher levels of IFN-gamma compared to Icam1(tm1Jcgr) and wild type mice. Thus, our investigations show that the alternatively spliced ICAM-1 isoforms are functional, and play key roles during the progression of CNS inflammation and demyelination in EAE. Furthermore, our findings suggest that these isoforms may also play key roles in controlling the development of inflammatory diseases such as multiple sclerosis, possibly through differential engagement with ICAM-1 ligands such as Mac-1.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Hu X,Barnum SR,Wohler JE,Schoeb TR,Bullard DCdoi
10.1016/j.molimm.2010.03.005subject
Has Abstractpub_date
2010-05-01 00:00:00pages
1692-1700issue
9eissn
0161-5890issn
1872-9142journal_volume
47pub_type
杂志文章abstract：:The envelope protein of the feline immunodeficiency virus (FIV) was analyzed using several epitope prediction programs based on profiles of hydrophilicity, antigenicity, and probability of residues to lie on the protein surface. Tentative homologies with the immunodominant epitope sites in simian virus (SIV) or human ...
journal_title：Molecular immunology
pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/j.molimm.2014.01.015
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2004.09.029
更新日期：2005-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：1996-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-10-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/s0161-5890(01)00042-6
更新日期：2001-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0161-5890(94)90167-8
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/0161-5890(95)00068-2
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/0161-5890(92)90001-e
更新日期：1992-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.04.023
更新日期：2008-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.02.012
更新日期：2007-07-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90043-5
更新日期：1987-06-01 00:00:00
