Abstract:
:An expression cDNA library was constructed from the helper T cell hybridoma, A.1.1, which has been shown to produce constitutively proteins involved in the down regulation of the immune response. From this library we identified and characterized a cDNA clone, J6B7, by screening with a polyclonal antibody specific for secreted immune regulatory proteins. The mRNA for J6B7 is expressed specifically in some T cells, but not in the thymoma BW5147 or liver cells. J6B7 is 2937 nucleotides in length and contains one open reading frame encoding for a peptide of predicted Mr of 98,042. The nucleotide and deduced amino acid sequences of J6B7 did not reveal significant homology to any published sequences. Hybridization and translation experiments reveal that the J6B7 can hybrid select mRNA from total RNA isolated from either A.1.1 cells or thymic tissue which can be translated in vitro to a peptide which is bound by a monoclonal antibody (mAb) specific for antigenic determinant(s) shared by immune regulatory proteins. Furthermore, the in vitro translated proteins obtained from A.1.1 cells and thymus showed significant suppression of a mixed lymphocyte reaction (MLR) in a dose dependent manner, reaching maximum suppression of 71% and 89%, respectively. These results suggest that the cDNA, J6B7, codes for an immune regulatory protein.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Lee C,Ghoshal K,Beaman KDdoi
10.1016/0161-5890(90)90102-6subject
Has Abstractpub_date
1990-11-01 00:00:00pages
1137-44issue
11eissn
0161-5890issn
1872-9142journal_volume
27pub_type
杂志文章abstract::Previous studies showed that P2 receptors are involved in neutrophil migration via stimulation of chemokine release and by facilitating chemoattractant gradient sensing. Here, we have investigated whether these receptors are involved in LPS-induced neutrophil transendothelial migration (TEM) using a Boyden chamber whe...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.11.020
更新日期:2010-02-01 00:00:00
abstract::Discordant xenografts surviving the initial hyperacute rejection phase may be subject to cellular rejection processes mediated by infiltrating leukocytes including T cells, NK cells and monocytes. The stable adhesion of these cell types to endothelial cells is due to the molecular interaction of the integrins VLA-4 an...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(97)00042-4
更新日期:1997-04-01 00:00:00
abstract::The anti-cytokeratin (CK) 8 monoclonal antibody (mab) TS1 has been shown to efficiently bind to CK8 expressed in carcinomas in vivo. The anti-idiotypic antibody of TS1, alphaTS1, can be used to regulate the tumor:non-tumor ratio of TS1 by clearing non-tumor binding TS1 from the circulation. If the interaction of TS1 t...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.08.001
更新日期:2007-02-01 00:00:00
abstract::This paper presents the initial characterization of a mouse monoclonal antibody (RH1-38) which blocks, in the absence of complement, three different systems of cell-mediated cytotoxicity. This monoclonal antibody markedly inhibits cytotoxicity mediated by human natural killer cells, a monocyte-like cell [phorbol myris...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90140-3
更新日期:1985-07-01 00:00:00
abstract::A recent report indicated that T200 molecules interact with elements of the cytoskeleton in BW5147 T lymphoma cells. We have confirmed the cytoskeletal association of T200 by examining nonionic detergent-soluble and detergent-insoluble fractions of murine T cell tumor cell lines, cloned cytotoxic T lymphocyte lines, a...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90111-9
更新日期:1989-10-01 00:00:00
abstract::Increasing lines of evidence indicate that arsenic may be associated with immune related problems, but its detailed effects on immune organs are poorly understood. The objective of this study was to explore inflammatory responses and T cell differentiation of arsenic exposure in spleen and thymus. Female C57BL/6 mice ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.12.005
更新日期:2017-01-01 00:00:00
abstract::The human C epsilon gene produces a number of alternatively spliced heavy chain transcripts of which some encode functional IgE isoforms. We now show that differentially processed epsilon mRNA variants also exist in the mouse and are generated by differential polyadenylation and alternative splicing of primary epsilon...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(96)00110-1
更新日期:1997-02-01 00:00:00
abstract::Mouse monoclonal antibodies (MAbs) have been prepared against rat T cell blasts. One MAb called MRC OX-40 recognized an antigen that differed from any previously described in that its expression was detected only on T blasts that also expressed the CD4 antigen. The OX-40 MAb did not detect an activation determinant of...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(87)90122-2
更新日期:1987-12-01 00:00:00
abstract:BACKGROUND:Published data on crustacean allergens are incomplete. The identification of tropomyosin (TM), arginine kinase (AK), sarcoplasmic Ca-binding protein (SCP) and myosin light chain (MLC) as shrimp allergens are all important contributions but additional allergens are required for the development of a complete s...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.06.216
更新日期:2011-09-01 00:00:00
abstract::Toll-like receptors (TLRs) are a family of highly conserved germline-encoded pattern-recognition receptors (PRRs) and are essential for host immune response. Little is known regarding the activation mechanism of TLRs especially of the TLR7/8/9 subfamily. Here we cloned and characterized bovine TLR8 (bTLR8) and found t...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.09.024
更新日期:2009-02-01 00:00:00
abstract::CD55 is a key regulator of complement activation, expressed on most tissues and cells in man and other mammals. In the rat, alternative splicing in the gene encoding CD55 yields GPI-anchored (GPI-CD55) and transmembrane (TM-CD55) forms. Published Northern blot analysis indicated that while GPI-CD55 was broadly express...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.08.018
更新日期:2007-03-01 00:00:00
abstract::Spondyloarthritis is the second most common form of chronic inflammatory arthritis and a unique hallmark of the disease is pathologic new bone formation. Several cytokine pathways have been genetically associated with ankylosing spondylitis (AS), the prototypic subtype of SpA, and additional evidence from human and an...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2013.07.016
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Individuals genetically deficient of properdin are more susceptible to meningococcal disease. Likewise low concentration or decreased biological activity of mannose-binding lectin (MBL) is associated with higher incidence of bacterial infections during childhood. In this study we report our findings in a Dan...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.02.017
更新日期:2006-02-01 00:00:00
abstract::The immunosuppressive cyclic undecapeptide cyclosporine (Cs) represents a useful model for studying the molecular basis of antibody-antigen interactions. The three-dimensional structure of the Cs molecule is known and a large panel of monoclonal antibodies (mAbs) to Cs has been well characterized by cross-reactivity s...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(90)90126-k
更新日期:1990-10-01 00:00:00
abstract::Interleukin-6 (IL-6) is a pleiotropic cytokine that regulates hematopoiesis, inflammation, immune responses and bone homeostasis in mammals. Fish IL-6 has been cloned in recent years but to date no functional studies have been reported. Thus, in this paper we present for the first time in fish the functional character...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.05.027
更新日期:2011-09-01 00:00:00
abstract::Mutations within the 5'-non-coding region of the bcl-6 gene can occur in lymphomas that originate from germinal centers (GCs), as well as in normal memory and GC B cells. Mutations in the p53 gene occur in 50% of human cancers. Since both bcl-6 and p53 can be mutated in certain circumstances, we investigated the accum...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(02)00117-7
更新日期:2002-11-01 00:00:00
abstract::NKG2D receptor-ligand interaction triggers NK cell-mediated cytolysis and IFN-gamma secretion. IFN-gamma produced by NK cells has been found to promote the interaction between NK cells and monocytes; however, the underlying mechanism remains elusive. We demonstrate here that IFN-gamma exclusively induced or upregulate...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.10.007
更新日期:2008-03-01 00:00:00
abstract::The molecular structure of protein and epitope mapping strategies are required to engineer the epitopes of a protein. In the present study, IgE binding regions of osmotin were identified and mutated to obtain hypoallergenic variant. The three dimensional (3-D) model of osmotin obtained by homology modeling comprised o...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.10.019
更新日期:2015-03-01 00:00:00
abstract::Peptides representing the amino-terminal sequence of protamines HP1 (sequence 1-12) and HP2 (sequence 1-11), the two major nuclear proteins of human sperm, have been synthesized. Rabbits were immunized either with peptide conjugated with a carrier or with free peptide. The resulting antisera were examined for their ca...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(91)90179-n
更新日期:1991-09-01 00:00:00
abstract::MiRNAs are a class of endogenous tiny RNAs that act as inhibitors of translation or promote RNA degradation by duplex-formation within the 3'-UTR of target mRNAs. They play an important role during a wide range of cellular processes by fine-tuning of gene expression. The differentiation of monocytes to macrophages pla...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.06.014
更新日期:2013-12-01 00:00:00
abstract::Infection of ruminants and humans with Fasciola gigantica is attracting increasing attention due to its economic impact and public health significance. However, little is known of innate immune responses during F. gigantica infection. Here, we investigated the expression profiles of genes involved in Toll-like recepto...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.12.007
更新日期:2018-02-01 00:00:00
abstract::Porphyromonas gingivalis causes periodontal diseases and its lipopolysaccharide (LPS) is considered as a major virulence factor responsible for pathogenesis. Since initial recognition of P. gingivalis LPS (Pg.LPS) in the oral cavity might be crucial for the host response, we identified Pg.LPS-binding proteins (Pg.LPS-...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.06.434
更新日期:2011-09-01 00:00:00
abstract::SCID can be caused by various genetic mutations leading to distinctive phenotypes according to the presence of T, B and NK cells. Artemis is a gene encoded on chromosome 10p. The deficiency of this molecule causes an inability to repair DNA double strand breaks and is one of the causes of radiosensitive T-B-NK+ SCID. ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.05.004
更新日期:2013-12-01 00:00:00
abstract::Crotoxin (Ctx) is the main lethal component of Crotalus durissus terrificus venom. It is a neurotoxin, composed of two subunits associated by noncovalent interactions, the non-toxic acid subunit (CA), named Crotapotin, and the basic subunit (CB), with phospholipase A2 (PLA2) activity. Employing the SPOT synthesis tech...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2020.01.012
更新日期:2020-03-01 00:00:00
abstract::Lewis (LEW) and Wistar Kyoto (WKY) rats of the same major histocompatibility complex (MHC) haplotype (RT.1(l)) display differential susceptibility to adjuvant-induced arthritis (AIA). LEW are susceptible while WKY are resistant to AIA. To gain insights into the mechanistic basis of these disparate outcomes, we compare...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.05.230
更新日期:2013-12-01 00:00:00
abstract::Macrophage migration is mediated by complex cellular signaling processes and cytoskeleton re-arrangement. In particular, recent advances indicate that the innate immunity signaling process plays a key role in the regulation of macrophage migration. In this report, we have provided evidence demonstrating the involvemen...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.12.004
更新日期:2010-03-01 00:00:00
abstract::Natural killer (NK) cells express receptors that are specific for MHC class I molecules. These receptors play a crucial role in regulating the lytic and cytokine expression capabilities of NK cells. In humans, three distinct families of genes have been defined that encode for receptors of HLA class I molecules. The fi...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(01)00107-9
更新日期:2002-02-01 00:00:00
abstract::Antigens related to the carcinoembryonic antigen (CEA) were isolated from normal human plasma by perchloric acid extraction, gel permeation chromatography and immunoaffinity chromatography using a monoclonal antibody with broad specificity and high affinity. The antigens were separated by SDS-polyacrylamide gel electr...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90046-x
更新日期:1985-11-01 00:00:00
abstract::A set of 58 overlapping rod-bound peptides was used to map the antigenic reactivity pattern of a 64-residue neurotoxin (AaH II) from the venom of the scorpion Androctonus australis hector. Five anti-toxin rabbit antisera were assayed serially for their capacity to bind to each peptide in the set. Six regions of antige...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90152-2
更新日期:1993-08-01 00:00:00
abstract::Increasing evidences indicate that 2-Methoxyestradiol (2ME2) plays an essential role in protecting against inflammatory responses. However, its effect on IgG immune complex (IC)-induced acute lung injury (ALI) remains enigmatic. In the study, by using i.p. administration of 2ME2, we evaluated its influence on IgG IC-i...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.02.007
更新日期:2017-05-01 00:00:00