Abstract:
:The potential biochemical mechanisms that mediate the 'shedding' of soluble IL-6 RI (80-kDa) receptor fragments in populations of adherent macrophages were explored. Stimulated macrophages displayed proportional increases in both the expression of membrane-associated IL-6 RI (80-kDa) and the release of soluble receptor fragments. The use of protease inhibitors implicated thiol/cysteine and carboxyl/aspartate proteases in this process. Cathepsin-D depleted membrane-associated IL-6 RI (80-kDa) complexes and generated soluble receptor fragments. A carboxyl/aspartate protease from activated macrophages isolated utilizing pepstatin-A affinity chromatography, was also found to affect membrane-associated IL-6 RI (80-kDa) complexes and generate soluble receptor fragments. Most likely, this fraction corresponded to cathepsin-D based upon its origin, pepstatin-A binding avidity, Hb-PAGE zymography, and hydrolysis of an enzyme-specific substrate. We conclude that cathepsin-D can generate soluble fragments of IL-6 RI (80-kDa) expressed by both macrophages and vascular endothelium.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Coyne CP,Howell T 3rd,Baravick J,Baravick E,Willetto C,Fenwick BWdoi
10.1016/s0161-5890(01)00074-8subject
Has Abstractpub_date
2001-09-01 00:00:00pages
347-57issue
5eissn
0161-5890issn
1872-9142pii
S0161589001000748journal_volume
38pub_type
杂志文章abstract::Recently, basophils have been suggested to produce inflammatory mediators in response to IgE in the absence of allergens. Monocyte chemoattractant protein-1 (MCP-1) plays an important role in the initiation of inflammatory responses by recruiting various immune cells to the site of allergic inflammation. In the presen...
journal_title:Molecular immunology
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journal_title:Molecular immunology
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pub_type: 杂志文章
doi:10.1016/j.molimm.2008.04.021
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2015-02-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2002-02-01 00:00:00
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更新日期:2010-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
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更新日期:2017-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:1996-08-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(84)90074-9
更新日期:1984-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(89)90032-1
更新日期:1989-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.molimm.2005.10.020
更新日期:2006-04-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.06.007
更新日期:2010-09-01 00:00:00
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journal_title:Molecular immunology
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