Abstract:
:This paper describes additional structural analyses of the p24 cell surface molecule recognized by monoclonal antibody BA-2. Since BA-2 is broadly reactive with a variety of normal and malignant lymphohematopoietic and nonlymphohematopoietic cells, we examined the structure of p24 expressed on different cell types. Tryptic peptide mapping and 2-dimensional gel electrophoresis of p24 isolated from colon carcinoma cells, fresh leukemic cells, leukemic cell lines, and activated T-cells indicated that p24 exhibits no structural polymorphism within the cells examined. As has recently been demonstrated with several other cell surface molecules, p24 is shown to possess a covalently-attached fatty acid, based on the incorporation of [3H]palmitate. We have also identified an additional protein, designated p26, that is coprecipitated with p24. The p26 molecule is not disulfide-linked to p24, and can be immunoprecipitated from a variety of 125I- or [35S]methionine-labeled cells. V8 protease peptide mapping indicated that p24 and p26 are structurally homologous. Pulse-chase analysis using [35S]methionine and digestion with endoglycosidase-F indicated that p24 and p26 are probably derived from a p23 precursor, but no precursor-product relationship exists between p24 and p26. Based on this data we propose that p24 and p26 are most likely differentially-processed protein products of the same gene.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
LeBien TW,Pirruccello SJ,McCormack RT,Bradley JGdoi
10.1016/0161-5890(85)90007-0subject
Has Abstractpub_date
1985-10-01 00:00:00pages
1185-94issue
10eissn
0161-5890issn
1872-9142journal_volume
22pub_type
杂志文章abstract::Zinc finger protein regulator of apoptosis and cell cycle arrest (Zac1) is a transcription factor able to induce apoptosis or cell cycle arrest through independent pathways. In spite of the important potential functions attributed to Zac1, little is known of its physiological regulation and biological function. We dis...
journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2010-11-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2005-02-01 00:00:00
abstract::Idiotypic-like interactions between mAbs directed against cytidine (Cyd) or guanosine (Guo) nucleosides were characterized. These mAbs, Cyd-1 (IgG2b, kappa), Guo-1 (IgG1, kappa) and Guo-2 (IgG1, kappa) were derived from splenocytes of A/J mice immunized with Cyd-KLH or Guo-KLH and recognized the nucleoside base moieti...
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(84)90143-3
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abstract::Cimetidine, a histamine type-2 receptor antagonist, is known to inhibit the growth of several tumors in human and animals, however the mechanism of action underlying this effect remains largely unknown. Here, in the mice model of 3LL lung tumor, cimetidine showed significant inhibition of tumor growth. However, an in ...
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pub_type: 杂志文章
doi:10.1016/0161-5890(89)90032-1
更新日期:1989-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.07.006
更新日期:2011-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(94)90081-7
更新日期:1994-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(86)90173-2
更新日期:1986-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2004.03.001
更新日期:2004-05-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(84)90092-0
更新日期:1984-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2013.08.015
更新日期:2014-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(83)90169-4
更新日期:1983-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0161-5890(95)00148-4
更新日期:1996-03-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2009.05.005
更新日期:2009-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
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更新日期:2009-10-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90040-i
更新日期:1993-10-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2019.11.003
更新日期:2020-01-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.02.017
更新日期:2006-02-01 00:00:00
abstract::Mugwort (Artemisia vulgaris) belongs to the Compositae family, and is one of the main causes of allergy in late summer and autumn. The aim of the study was to characterize the allergen Art v 2 from mugwort pollen. Skin prick tests, performed in 19 patients allergic to mugwort and 10 control patients, showed an Art v 2...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2007.04.022
更新日期:2007-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(02)00090-1
更新日期:2002-09-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(00)00091-2
更新日期:2000-08-01 00:00:00
abstract::Human leukocyte antigens (HLA) allele determination is becoming an increasingly important aspect in the field of transplantation as well as in the area of HLA association with a number of diseases. Through Lebanon's history, this country, situated at a crossroads between Europe, Asia and Africa, has been a host for va...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(03)00073-7
更新日期:2003-07-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2006.09.031
更新日期:2007-03-01 00:00:00
abstract::A channel catfish genomic lambda library was screened with CH and JH probes which were derived from our earlier sequence analyses on different full-length heavy chain cDNA clones. One clone, designated C7, contained a genomic insert of about 18 kb and hybridized with specific probes for each of the four domains of the...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(92)90096-g
更新日期:1992-02-01 00:00:00
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journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2017.09.016
更新日期:2018-01-01 00:00:00